Objective To explore the expression of nestin-1(NID1)in clear cell renal cell carcinoma(ccRCC)and its effect on angiogenesis.Methods The expression of NID1 in ccRCC was analyzed using data from the TCGA database,cell lines and clinical samples.Additionally,the relationship between NID1 and the angiogenesis marker CD31 was investigated.A stable knockdown 786-O cell line with reduced NID1 expression was generated through lentiviral transfection.The conditioned medium obtained from these cells was used to treat human umbilical vein endothelial cells(HUVECs).HUVEC cells were divided into four groups:the DMEM group,the blank group,the NID1 empty vector group and the NID1 knockdown group.Cell proliferation ability was assessed using CCK-8 method,while tube formation ability and migration ability were evaluated through tube formation test and scratch test respectively.A mouse model of dextran hydrogel unloaded/NID1 knockdown tumor cell complex culture system was established and divided into the unloaded NID1 group and the NID1 knockdown group.Immunohistochemical staining was performed to detect the expression of CD31.Results Bioinformatics analysis revealed an increased expression of NID1 in ccRCC tissue.Furthermore,500 differentially expressed genes positively correlated with elevated levels of NID1 were primarily enriched in angiogenesis-related processes.The results showed a positive correlation between the expression of NID1 and CD31 in cancer tissue.After treatment with conditioned medium derived from the knockdown group,HUVEC cells exhibited weakened migration and tube formation abilities in the empty vector control group(P＜0.05),while their proliferation ability remained unchanges compared to the empty vector control group(P＞0.05).In vivo experiments using a nude mouse model,the expression of CD31 demonstrated significantly lower levels in the NID1 knockdown group(P＜0.05).Conclusion The expression of NID1 is increased in ccRCC,and it can promote angiogenesis.

Objective:To explore the application effect of PBL teaching guided by anesthesia plan in clinical teaching of anesthesia undergraduate practice.Methods:A total of 34 anesthesiology undergraduates who practiced in Daqing Oilfield General Hospital/The Tenth Affiliated Hospital of Qiqihar Medical University from January 2020 to September 2020 were selected and randomly divided into experimental group ( n=17) and control group ( n=17). The experimental group adopted PBL teaching guided by anesthesia plan, while the control group adopted traditional teaching. The theoretical assessment results, case analysis assessment results, and clinical skills assessment results of the two groups of undergraduate interns were compared and analyzed. Additionally, a questionnaire survey was conducted to evaluate the students' comprehensive ability. SPSS 22.0 was used for t test. Results:The scores and total scores of theoretical knowledge and anesthesia skills in the experimental group were higher than those in the control group, and the difference was statistically significant ( P<0.05). Before learning, there was no significant difference in scores and total scores of comprehensive ability between the two groups ( P>0.05); after learning, the comprehensive ability of the two groups of students were improved, and those of the experimental group were higher than those of the control group ( P<0.05). Conclusion:PBL teaching guided by anesthesia plan can effectively improve the mastery of theoretical knowledge, clinical operation skills and case analysis ability of anesthesia undergraduate interns, and the teaching effect is good, which is worthy of further promotion.