Objective:To explore the role of constructivism teaching theory combined with problem-based learning (PBL) teaching mode in the training of new nurses in the department of gastroenterology.Methods:A total of 27 newly recruited nurses in the Department of Gastroenterology in West China Hospital of Sichuan University from August 2018 to August 2019 were selected as the control group, and traditional teaching was adopted. In addition, 24 newly recruited nurses from September 2019 to September 2020 were selected as the research group, using constructivism teaching theory combined with PBL teaching mode. After the training, the teaching effect was compared between the two groups through the assessment results and the training method scores. SPSS 19.0 was used for t test. Results:The research group's newly recruited nurses' theoretical evaluation scores [(95.08±5.61) vs. (91.63±5.27)] and practical skills operation evaluation scores [(94.30±7.22) vs. (87.13±6.84)] were both higher than those of the control group, and the difference was statistically significant ( P<0.05). Compared with the control group, the newly recruited nurses of the research group believed that the combination of constructivism teaching theory and PBL teaching could improve clinical thinking ability, analysis and problem-solving ability, and the degree of integration of theory and practice, mobilize the enthusiasm of autonomous learning, enhance autonomous learning ability, and improve communication ability, cultivate teamwork ability, stimulate interest in learning, improve work confidence and training satisfaction, with statistical significance ( P<0.05). Conclusion:The combination of constructivism teaching theory and PBL teaching mode is beneficial to the training of newly recruited nurses in the department of gastroenterology to independently construct a theoretical framework of knowledge, improve comprehensive ability, and make a useful exploration for the training of newly recruited nurses.

In recent years,the application of humanities curricula has gained increasing attention gradually in Chinese medical education.For graduate students in oncology who have just been exposed to the actual clinical work,it is extremely important to develop the comprehensive abilities of physician-patient communication,humanistic care and self psychological dredging.Since 2012,we have conducted humanities curriculum by narrative-based instruction for the clinical intern graduate students in oncology at the department of surgical oncology in the First Affiliated Hospital of Xi'an Jiaotong University,and a good teaching result has been gained.This kind of humanities curricula based on open discussion could be an effective way to improve physician-patient communication ability and reduce the clinical psychology pressure for the graduate students in oncology.

OBJECTIVETo screen the genes related to cell cycle under regulation by KIAA0101 in gastric cancer.

METHODSRT-PCR was used to detect the expression level of KIAA0101 gene in gastric cancer tissue and paired adjacent tissues. GO function enrichment analysis and KEGG pathway enrichment analysis were carried out using DAVID database. KEGG was used to map the pathways and the corresponding genes were analyzed. The list of genes associated with the KIAA0101 expression pattern was imported into TCGA cBioPortal to analyze the relationship between the interacting genes and generate a genetic topology map. The candidate genes were screened by RT-PCR.

RESULTSThe expression level of KIAA0101 mRNA was significantly higher in cancer tissues than in paired adjacent tissues (1.104 ± 0.379 0.421 ± 0.172; =0.0179). The system screened genes related with KIAA0101 from 478 tissues by pooled analysis of the expression intensity of all the gene probes. GO function analysis showed that the differential genes were mainly enriched in protein phosphorylation, RNA processing, cell cycle, DNA metabolism, protein transport, acetylation, apoptosis, proteolysis, and redox. The changes in the expression level of KIAA0101 mainly affect the gastric cancer-related pathways including cell cycle, spliceosome, DNA replication, and p53 signal transduction pathway. KEGG pathway maps and gene topology maps showed that the genes related to KIAA0101 (such as BUB1B, MAD2L1, CDC45, CDK1, CCNE1 and CCNB2) were also related to cell cycle. RT-PCR results confirmed significant increments of the expression levels of BUB1B, MAD2L, CDK1, CCNE1, and CCNB2 mRNA in gastric cancer tissues as compared with the paired adjacent gastric tissues ( < 0.05), but CDC45 mRNA did not show significant differential expression in gastric cancer tissues ( > 0.05).

CONCLUSIONSKIAA0101 may affect cell cycle by regulating the expression of BUB1B, MAD2L1, CDK1, CCNE1 and CCNB2, and this finding may provide evidence for understanding how KIAA0101 affects cell cycle and for screening of tumor markers and selection of drug targets.

OBJECTIVETo investigate and improve the diagnosis and management of small-cell neuroendocrine carcinoma of the stomach (SCNECS).

METHODSThe clinicopathological information and survival data of 21 cases of SCNECS treated in our hospital from January 2003 to December 2012 were analyzed retrospectively.

RESULTSThe median survival time of the 21 cases was (12.1±1.6) months. The 1-year overall survival rate of the patients was 33.3%. Univariate analysis showed that the risk factors of survival were tumor size, lymph node status, tumor stage, treatment and radical operation or not (P<0.05 for all). Multivariate analysis indicated that independent risk factors were tumor size ≥4.6 cm, lymph node metastasis and tumor stage III/IV (P<0.05 for all). Radical operation and comprehensive treatment (surgery + postoperative chemotherapy) were independent protective factors (all P<0.05).

CONCLUSIONSSCNECS is a rare malignant tumor with early metastasis and poor prognosis. Tumor size, stage, lymph node status, and treatment have potential impact on the prognosis. Comprehensive treatment based on radical operation may improve the survival of SCNECS patients.

Carcinoma, Neuroendocrine ; diagnosis ; Carcinoma, Small Cell ; diagnosis ; Humans ; Lymph Node Excision ; Lymphatic Metastasis ; Prognosis ; Retrospective Studies ; Stomach ; Stomach Neoplasms ; diagnosis ; Survival Rate

Objective To investigate changes in the firing activities of nucleus accumbens (NAc)neurons and their response to 5-HT7 receptor stimulation in a rat model of Parkinson’s disease (PD).Methods The firing activities and response of NAc neurons to 5-HT7 receptor agonist in PD rats were recorded by in vivo electroneurophysiology and neuropharmacology and then were compared with those in the sham group.Results The mean firing rate of NAc neurons was (5.46 ±0.88)Hz in the sham rats and (3.77 ±0.48)Hz in the PD rats. The firing rate of NAc neurons increased significantly compared with that in the sham rats (P <0.05).In PD rats, 65% of NAc neurons fired in bursts and 35% fired irregularly.However,in the sham rats,57.5% of NAc neurons fired in bursts and 42.5% fired irregularly.There was no significant difference in the firing pattern of NAc neurons between the PD and sham rats (P >0.05 ).Systemic administration of 5-HT7 receptor agonist AS1 9 increased the firing rate of NAc neurons in the sham and PD rats.This excitation was significant at a high dose of 1 60 μg/kg for NAc neurons in the sham rats (P <0.05).However,the excitation produced by AS1 9 was significant at a high dose of 80 μg/kg in PD rats (P <0.05).The cumulative dose-produced excitation in the PD rats was lower than that in the sham rats.The effects induced by AS1 9 were reversed by the 5-HT7 receptor antagonist SB269970 in both groups.Conclusion The reinforced firing activity of NAc neurons might be mediated by 5-HT7 receptor in the neurons of PD rats.

Objective To investigate and improve the diagnosis and management of small-cell neuroendocrine carcinoma of the stomach (SCNECS).Methods The clinicopathological information and survival data of 21 cases of SCNECS treated in our hospital from January 2003 to December 2012 were analyzed retrospectively.Results The median survival time of the 21 cases was (12.1 ±1.6) months.The 1-year overall survival rate of the patients was 33.3%.Univariate analysis showed that the risk factors of survival were tumor size, lymph node status, tumor stage, treatment and radical operation or not (P<0.05 for all).Multivariate analysis indicated that independent risk factors were tumor size≥4.6 cm, lymph node metastasis and tumor stage Ⅲ/Ⅳ ( P <0.05 for all).Radical operation and comprehensive treatment ( surgery +postoperative chemotherapy) were independent protective factors ( all P<0.05) .Conclusions SCNECS is a rare malignant tumor with early metastasis and poor prognosis.Tumor size, stage, lymph node status, and treatment have potential impact on the prognosis.Comprehensive treatment based on radical operation may improve the survival of SCNECS patients.

Objective To investigate and improve the diagnosis and management of small-cell neuroendocrine carcinoma of the stomach (SCNECS).Methods The clinicopathological information and survival data of 21 cases of SCNECS treated in our hospital from January 2003 to December 2012 were analyzed retrospectively.Results The median survival time of the 21 cases was (12.1 ±1.6) months.The 1-year overall survival rate of the patients was 33.3%.Univariate analysis showed that the risk factors of survival were tumor size, lymph node status, tumor stage, treatment and radical operation or not (P<0.05 for all).Multivariate analysis indicated that independent risk factors were tumor size≥4.6 cm, lymph node metastasis and tumor stage Ⅲ/Ⅳ ( P <0.05 for all).Radical operation and comprehensive treatment ( surgery +postoperative chemotherapy) were independent protective factors ( all P<0.05) .Conclusions SCNECS is a rare malignant tumor with early metastasis and poor prognosis.Tumor size, stage, lymph node status, and treatment have potential impact on the prognosis.Comprehensive treatment based on radical operation may improve the survival of SCNECS patients.