The key to improving the cultivation quality of socialist core values in medical colleges and universities， enhancing their dissemination efficiency， consolidating the foundation of medical students’ ideals and beliefs， and uniting the spiritual strength of medical students to serve society and dedicate themselves to the people， lies in integrating the red doctor spirit into the cultivation process of medical students’ socialist core values. Faced with the dilemmas encountered in the cultivation of socialist core values in medical colleges and universities， this paper traced the connection between the red doctor spirit and the socialist core values and reflected on the profound value connotations after the integration of the two， thereby providing theoretical references for finding appropriate cultivation methods and approaches in the future.

Objective:To prepare and screen monoclonal antibodies against Herpes simplex virus-1(HSV-1),and develop a double antibody sandwich quantitative enzyme-linked immunosorbent assay( Q-ELISA) for detection of HSV-1 particle. This method was used to control the quality of viral particle in the developing and manufacturing process of HSV-1. Methods: BALB/c mice was immunized with HSV-1 to prepare monoclonal antibodies. A double antibody sandwich Q-ELISA was developed to determine concentration of HSV-1 particle,which was based on the neutralizing monoclonal antibody 1F6 as capture antibody,and 2B1 as HRP-conjugated antibody. The performance of the reagent was evaluated,including specificity,sensitivity,precision,accuracy and linear. And the relation between the amount of virus detected by this method and the virus titer was analyzed by regression analysis method. Results: The Q-ELISA for HSV-1 particle was developed. The quantitation scope was 0. 125-2 μg/ml, the coefficient correlation was 0. 995 5, the limit of detection was 0. 125 μg/ml, the recovery was between 85. 6% and 107. 1%, the variation coefficient was lower than 10%, and the reagent does not react with other samples except HSV-1 antigen. This method has a good correlation with virus titer. Conclusion:The Q-ELISA for HSV-1 particle was successfully developed,which provide a new approach for rapid and quantitative detection of HSV-1 antigen.