Objective Based on performance assessment indicators for tertiary public hospitals,a retrospec-tive analysis was carried out on the surgical medical quality and operational efficiency of a specific tertiary A-level public hospital for the first quarter of 2024.The analysis yielded recommendations for the advancement of hospital sur-gical departments.Methods The comprehensive evaluation of the surgical performance level of a certain tertiary A-level public hospital was conducted using the weighted TOPSIS method,the rank sum ratio(RSR)method,and a fuzzy combination of the two methods.Results For the first quarter of 2024,A12,A13,and A6 were ranked in the top three by the complete review,while A3,A22,and A1 were listed in the bottom three.The majority of depart-ments fell into the middle.The evaluation's findings mainly matched the hospital departments'real state of develop-ment,showing variations in each department's performance.Conclusion The overall performance level of the surgical department in the tertiary public general hospital needs improvement.The results of surgical performance are signifi-cantly impacted by"functional positioning".In order to raise performance levels,technical innovation and efficiency optimisation should be prioritised,along with a greater attention on different components.

ObjectiveDetection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability, health, and metabolic rate.After exposure to environmental stimuli, both the internal reference gene and target gene would be degraded. As a result, it is imperative to consider the accurate capture of nascent RNA and the detection of transcriptional levels of RNA following environmental stimulation. This study aims to create a Click Chemistry method that utilizes its property to capture nascent RNA from total RNA that was stimulated by the environment. MethodsThe new RNA was labeled with 5-ethyluridine (5-EU) instead of uracil, and the azido-biotin medium ligand was connected to the magnetic sphere using a combination of “Click Chemistry” and magnetic bead screening. Then the new RNA was captured and the transcription rate of 16S rRNA was detected by fluorescence molecular beacon (M.B.) and quantitative reverse transcription PCR (qRT-PCR). ResultsThe bacterial nascent RNA captured by “Click Chemistry” screening can be used as a reverse transcription template to form cDNA. Combined with the fluorescent molecular beacon M.B.1, the synthesis rate of rRNA at 37℃ is 1.2 times higher than that at 15℃. The 16S rRNA gene and cspI gene can be detected by fluorescent quantitative PCR,it was found that the measured relative gene expression changes were significantly enhanced at 25℃ and 16℃ when analyzed with nascent RNA rather than total RNA, enabling accurate detection of RNA transcription rates. ConclusionCompared to other article reported experimental methods that utilize screening magnetic columns, the technical scheme employed in this study is more suitable for bacteria, and the operation steps are simple and easy to implement, making it an effective RNA capture method for researchers.

Objective To evaluate the diagnostic value of histopathological examination of ultrasound-guided puncture biopsy samples in extrapulmonary tuberculosis(EPTB). Methods This study was conducted at the Shanghai Public Health Clinical Center.A total of 115 patients underwent ultrasound-guided puncture biopsy,followed by MGIT 960 culture(culture),smear,GeneXpert MTB/RIF(Xpert),and histopathological examination.These assays were performed to evaluate their effectiveness in diagnosing EPTB in comparison to two different diagnostic criteria:liquid culture and composite reference standard(CRS). Results When CRS was used as the reference standard,the sensitivity and specificity of culture,smear,Xpert,and histopathological examination were(44.83%,89.29%),(51.72%,89.29%),(70.11%,96.43%),and(85.06%,82.14%),respectively.Based on liquid culture tests,the sensitivity and specificity of smear,Xpert,and pathological examination were(66.67%,72.60%),(83.33%,63.01%),and(92.86%,45.21%),respectively.Histopathological examination showed the highest sensitivity but lowest specificity.Further,we found that the combination of Xpert and histopathological examination showed a sensitivity of 90.80%and a specificity of 89.29%. Conclusion Ultrasound-guided puncture sampling is safe and effective for the diagnosis of EPTB.Compared with culture,smear,and Xpert,histopathological examination showed higher sensitivity but lower specificity.The combination of histopathology with Xpert showed the best performance characteristics.

Objective Based on performance assessment indicators for tertiary public hospitals,a retrospec-tive analysis was carried out on the surgical medical quality and operational efficiency of a specific tertiary A-level public hospital for the first quarter of 2024.The analysis yielded recommendations for the advancement of hospital sur-gical departments.Methods The comprehensive evaluation of the surgical performance level of a certain tertiary A-level public hospital was conducted using the weighted TOPSIS method,the rank sum ratio(RSR)method,and a fuzzy combination of the two methods.Results For the first quarter of 2024,A12,A13,and A6 were ranked in the top three by the complete review,while A3,A22,and A1 were listed in the bottom three.The majority of depart-ments fell into the middle.The evaluation's findings mainly matched the hospital departments'real state of develop-ment,showing variations in each department's performance.Conclusion The overall performance level of the surgical department in the tertiary public general hospital needs improvement.The results of surgical performance are signifi-cantly impacted by"functional positioning".In order to raise performance levels,technical innovation and efficiency optimisation should be prioritised,along with a greater attention on different components.

Objective Based on performance assessment indicators for tertiary public hospitals,a retrospec-tive analysis was carried out on the surgical medical quality and operational efficiency of a specific tertiary A-level public hospital for the first quarter of 2024.The analysis yielded recommendations for the advancement of hospital sur-gical departments.Methods The comprehensive evaluation of the surgical performance level of a certain tertiary A-level public hospital was conducted using the weighted TOPSIS method,the rank sum ratio(RSR)method,and a fuzzy combination of the two methods.Results For the first quarter of 2024,A12,A13,and A6 were ranked in the top three by the complete review,while A3,A22,and A1 were listed in the bottom three.The majority of depart-ments fell into the middle.The evaluation's findings mainly matched the hospital departments'real state of develop-ment,showing variations in each department's performance.Conclusion The overall performance level of the surgical department in the tertiary public general hospital needs improvement.The results of surgical performance are signifi-cantly impacted by"functional positioning".In order to raise performance levels,technical innovation and efficiency optimisation should be prioritised,along with a greater attention on different components.

Objective Based on performance assessment indicators for tertiary public hospitals,a retrospec-tive analysis was carried out on the surgical medical quality and operational efficiency of a specific tertiary A-level public hospital for the first quarter of 2024.The analysis yielded recommendations for the advancement of hospital sur-gical departments.Methods The comprehensive evaluation of the surgical performance level of a certain tertiary A-level public hospital was conducted using the weighted TOPSIS method,the rank sum ratio(RSR)method,and a fuzzy combination of the two methods.Results For the first quarter of 2024,A12,A13,and A6 were ranked in the top three by the complete review,while A3,A22,and A1 were listed in the bottom three.The majority of depart-ments fell into the middle.The evaluation's findings mainly matched the hospital departments'real state of develop-ment,showing variations in each department's performance.Conclusion The overall performance level of the surgical department in the tertiary public general hospital needs improvement.The results of surgical performance are signifi-cantly impacted by"functional positioning".In order to raise performance levels,technical innovation and efficiency optimisation should be prioritised,along with a greater attention on different components.

Objective Based on performance assessment indicators for tertiary public hospitals,a retrospec-tive analysis was carried out on the surgical medical quality and operational efficiency of a specific tertiary A-level public hospital for the first quarter of 2024.The analysis yielded recommendations for the advancement of hospital sur-gical departments.Methods The comprehensive evaluation of the surgical performance level of a certain tertiary A-level public hospital was conducted using the weighted TOPSIS method,the rank sum ratio(RSR)method,and a fuzzy combination of the two methods.Results For the first quarter of 2024,A12,A13,and A6 were ranked in the top three by the complete review,while A3,A22,and A1 were listed in the bottom three.The majority of depart-ments fell into the middle.The evaluation's findings mainly matched the hospital departments'real state of develop-ment,showing variations in each department's performance.Conclusion The overall performance level of the surgical department in the tertiary public general hospital needs improvement.The results of surgical performance are signifi-cantly impacted by"functional positioning".In order to raise performance levels,technical innovation and efficiency optimisation should be prioritised,along with a greater attention on different components.

Objective Based on performance assessment indicators for tertiary public hospitals,a retrospec-tive analysis was carried out on the surgical medical quality and operational efficiency of a specific tertiary A-level public hospital for the first quarter of 2024.The analysis yielded recommendations for the advancement of hospital sur-gical departments.Methods The comprehensive evaluation of the surgical performance level of a certain tertiary A-level public hospital was conducted using the weighted TOPSIS method,the rank sum ratio(RSR)method,and a fuzzy combination of the two methods.Results For the first quarter of 2024,A12,A13,and A6 were ranked in the top three by the complete review,while A3,A22,and A1 were listed in the bottom three.The majority of depart-ments fell into the middle.The evaluation's findings mainly matched the hospital departments'real state of develop-ment,showing variations in each department's performance.Conclusion The overall performance level of the surgical department in the tertiary public general hospital needs improvement.The results of surgical performance are signifi-cantly impacted by"functional positioning".In order to raise performance levels,technical innovation and efficiency optimisation should be prioritised,along with a greater attention on different components.

Objective Based on performance assessment indicators for tertiary public hospitals,a retrospec-tive analysis was carried out on the surgical medical quality and operational efficiency of a specific tertiary A-level public hospital for the first quarter of 2024.The analysis yielded recommendations for the advancement of hospital sur-gical departments.Methods The comprehensive evaluation of the surgical performance level of a certain tertiary A-level public hospital was conducted using the weighted TOPSIS method,the rank sum ratio(RSR)method,and a fuzzy combination of the two methods.Results For the first quarter of 2024,A12,A13,and A6 were ranked in the top three by the complete review,while A3,A22,and A1 were listed in the bottom three.The majority of depart-ments fell into the middle.The evaluation's findings mainly matched the hospital departments'real state of develop-ment,showing variations in each department's performance.Conclusion The overall performance level of the surgical department in the tertiary public general hospital needs improvement.The results of surgical performance are signifi-cantly impacted by"functional positioning".In order to raise performance levels,technical innovation and efficiency optimisation should be prioritised,along with a greater attention on different components.

Objective:Evaluate the effects of brief mindfulness meditation training on improving negative e-motions,mindfulness attention awareness,and sleep quality in patients after percutaneous coronary intervention(PCI)for coronary heart disease.Methods:Eighty-four patients with coronary heart disease after PCI admitted to the cardiology department were selected.According to the principle of simple randomization,they were divided into an intervention group of 42 cases and a control group of 42 cases.The Self Rating Anxiety Scale/Self Rating De-pression Scale,Mindfulness Attention Awareness Scale,and Pittsburgh Sleep Quality Index Scale were used as eval-uation indicators before and after intervention.Results:The difference in total scores of anxiety,depression,and mindfulness attention awareness before and after intervention in the intervention group was higher than that in the control group(P＜0.05).The difference in total sleep score,sleep quality,time to fall asleep,sleep duration,use of hypnotic drugs,daytime dysfunction,and total sleep score before and after intervention was higher than that of the control group(P＜0.05).Conclusion:It suggests that brief mindfulness meditation training could alleviate negative emotions in patients with coronary heart disease after PCI,improve mindfulness awareness,and improve sleep quali-ty.