Objective To explore the level of inflammatory factors and their correlation with prognosis in chronic cervicitis patients with human papillomavirus(HPV)infection.Methods Totally 100 patients from January 2022 to December 2023 were selected as the observation group.Among them,67 patients were 100 patients with low-risk HPV and 33 patients with high-risk HPV.At the same time,100 female personnel with physical examination in our hospital were selected as the control group.All of the patients were treated routinely.The C-reactive protein(CRP),interleukin(IL)-6,and serum amyloid A(SAA)levels were measured,and the patients were followed up for 6 months after treatment to observe the prognosis.Results Compared with the control group,The expression of CRP,IL-6 and SAA level increased in the observation group(P<0.05),Compared with the low-risk HPV group,The expression of CRP,IL-6 and SAA level increased in high-risk HPV group(P<0.05).Compared with patients with a good prognosis,Higher expression of CRP,IL-6 and SAA in patients with poor prognosis(P<0.05).The positive correlation between CRP,IL-6,SAA and low-risk HPV and high-risk HPV,Statistical difference(P<0.05).Negative correlation between CRP,IL-6,SAA and good prognosis,All showed a positive correlation between CRP,IL-6,and SAA and poor prognosis,There were statistical differences(P<0.05).Receiver operating characteristic(ROC)curve analysis showed that the diagnostic value of CRP,IL-6 and SAA(P<0.05).Conclusion Patients with chronic cervicitis have increased CRP,IL-6 and SAA in HPV infection,and the abnormal increased or decreased expression level is the risk predictor of chronic cervicitis with HPV infection,which may be closely related to the diagnosis of the disease,pathological characteristics,disease development and prognosis.

Objective: To elucidate the anti-inflammatory mechanism of Reduning Injection (RDN) by analyzing the potential biomarkers and metabolic pathways of the carrageenan-induced inflammatory model from the overall metabolic level. Methods: Rat inflammatory model was established by carrageenan. UPLC-Q-TOF/MS was used to detect and analyze changes of endogenous metabolites in the serum and urine of carrageenan-induced inflammatory rats. Combined with multivariate analysis and databases analysis, inflammatory-related potential biomarkers were screened and identified to analyze possible metabolic pathways. The reliability and biological significance of these biomarkers was verified by metabolic network analysis and correlation analysis with pharmacodynamic indicators. Results: A total of 16 potential biomarkers were screened and identified by multivariate analysis and metabolite databases, among which 13 species could be adjusted by RDN. The metabolism pathway analysis revealed that histidine metabolism, sphingolipid metabolism, and tyrosine metabolism were greatly disturbed. Their biomarkers involved urocanic acid, sphingosine, and norepinephrine, all of which showed a callback trend after RDN treatment. The three biomarkers had a certain correlation with some known inflammatory-related small molecules (histamine, arachidonic acid, Leukotriene B4, and PGE

Objective    To evaluate whether long frozen elephant trunk (FET) increases the risk of spinal cord injury in patients with acute type A aortic dissection. Methods    From 2018 to 2019, 172 patients with acute type A aortic dissection were treated in Guangdong Provincial People’s Hospital. They were divided into two groups according to the length of FET: patients treated with stents of 100 mm in length were enrolled into a short FET group, and those with stents of 150 mm in length into a long FET group. There were 124 patients in the short FET group, including 108 (87.1%) males and 16 (12.9%) females with a mean age of 51.8±7.9 years. There were 48 patients in the long FET group, including 44 (91.7%) males and 4 (8.3%) females with a mean age of 50.6±9.7 years. The clinical data and prognosis of the patients were analyzed. Results    The mean distal stent graft was at the level of T 8.5±0.7 in the long FET group, and at the level of T 6.8±0.6 in the short FET group (P=0.001). Sixteen patients died after operation in the two groups, including 13 (10.5%) in the short FET group and 3 (6.2%) in the long FET group (P=0.561). There were 7 patients of spinal cord injury in the two groups, including 6 (4.8%) in the short FET group and 1 (2.2%) in the long FET group (P=0.675). There was no statistical difference in other complications between the two groups. The follow-up time was 16.7 (1-30) months. During the follow-up, 2 patients died in the long FET group and 5 died in the short FET group. No new spinal cord injury or distal reintervention occurred during the follow-up. Conclusion    Long FET does not increase the incidence of spinal cord injury in patients with acute type A aortic dissection.

Objective:To investigate the kinetic metrics of 68Ga-fibroblast activation protein inhibitor (FAPI)-04 in pancreatic cancers and normal organs by using total-body PET dynamic imaging. Methods:From December 2020 to December 2021, 68Ga-FAPI-04 total-body PET/CT dynamic imaging were performed on 6 pancreatic cancer patients (3 males, 3 females, median age 55.5 years) in Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University. Images were respectively analyzed. Manual delineations of volume of interests (VOIs) on multiple normal organs and pathological lesions were performed and time-to-activity curves (TACs) were generated. A reversible two-tissue compartment model (2TCM) was fitted for each tissue TAC. Rate constants including K1, k2, k3 and k4, and the total volume of distribution ( Vt) were obtained and compared by tissue types. Wilcoxon rank sum test and Spearman correlation analysis were used for data analysis. Results:Kinetic metrics varied significantly among normal organs and pancreatic cancer lesions ( z values: 2.00-1 240.00, all P<0.05). The highest K1 among lesions was observed in primary tumor (0.30 min -1), which was observed in the spleen (1.42 min -1) among normal organs. The highest k2 among lesions was observed in peritoneal metastases (0.24 min -1), which was observed in the spleen (2.59 min -1) among normal organs. Primary tumor showed the highest k3 of 0.17 min -1 among lesions, and the pancreas had the highest k3 of 0.16 min -1 among normal organs. Primary tumor had the highest k4 of 0.03 min -1 among lesions, and the heart, lungs, parotid glands had high k4(0.06 min -1) among normal organs. Vt were higher in pathological lesions compared to normal organs, with the highest in primary tumor (13.78 ml/cm 3). There were correlations between Vt in lesions and SUV mean( rs=0.86, P<0.001) or SUV max ( rs=0.77, P<0.001). Conclusion:The rate constants including K1, k2, k3 and k4, and Vt of 68Ga-FAPI-04 vary among normal organs and lesions.

Objective:To investigate the clinical effect of nanofat transplantation combined with CO 2 fractional laser in the treatment of depressed facial scars. Methods:Through a retrospective study, a total of 27 patients with depressed facial scars who got admitted to the plastic surgery department of our hospital from January 2016 to June 2018 were recruited for autologous nanofat transplantation and carbon dioxide fractional laser treatment in combination to treat moderate to severe depressed facial scars. The treatment was carried out as follows: autologous nanofat transplantation was performed, the depressed scar was subcutaneously separated with a 26 G needle, and injected with the autologous nanofat 0.5-1.0 ml/cm 2. After 3 months, the fractional CO 2 laser treatment was performed for a total of 2 times, with an interval of 3 months each time. Before and six months after treatment, patients and surgeons used the Patient and Observer Scar Assessment Scale (POSAS) to evaluate each aspect of scar. The total score for each item was 1-10 points. According to the total POSAS scores of patients and doctors, the degree of improvement of depressed scars was classified and evaluated. Grade 1: invalid, total score reduction ≤ 5 points; grade 2: mildly effective, total score reduction> 5 points and ≤ 10 points; grade 3, effective, total score reduction> 10 points and ≤ 20 points; grade 4, markedly effective, total score reduction> 20 points. Using SPSS 23.0 to do t-test on the data before and after treatment. P<0.05 indicates that the difference is statistically significant. Results:A total of 27 patients were enrolled, including 16 males and 11 females, aged (29.1±7.9) years old. Before and after six months of treatment, the difference between the scores of the patients on all indicators of scar was statistically significant ( P<0.05); the difference between the doctor’s scores was statistically significant ( P<0.01). The total scores of post-total POSAS were (43.7±10.8) and (23.3±3.2) points respectively, the difference was statistically significant ( P<0.01). After six months of treatment, the degree of pain, itching, color, hardness, thickness (depression), elasticity, tension of the depressed scar were significantly improved. Among them, there was 1 case of second-level improvement, 14 cases of third-level improvement, and 12 cases of fourth-level improvement. The total effective rate reached 96.3% (26/27). None of the 27 patients had lumps or cord-like indurations on the filling parts of the face. No embolism, infection, hematoma and other complications occurred after nanofat transplantation in all patients. Skin flushing, burning, edema, pain and other symptoms occurred early after the fractional CO 2 laser treatment, and no hyperpigmentation occurred in the 6-month follow-up after a treatment cycle. Conclusions:The combination of nanofat transplantation technology and carbon dioxide fractional CO 2 laser can produce a significant effect on improving depressed scar, reducing treatment period, rendering a simple operation with few complications.

Objective:To investigate the clinical effect of nanofat transplantation combined with CO 2 fractional laser in the treatment of depressed facial scars. Methods:Through a retrospective study, a total of 27 patients with depressed facial scars who got admitted to the plastic surgery department of our hospital from January 2016 to June 2018 were recruited for autologous nanofat transplantation and carbon dioxide fractional laser treatment in combination to treat moderate to severe depressed facial scars. The treatment was carried out as follows: autologous nanofat transplantation was performed, the depressed scar was subcutaneously separated with a 26 G needle, and injected with the autologous nanofat 0.5-1.0 ml/cm 2. After 3 months, the fractional CO 2 laser treatment was performed for a total of 2 times, with an interval of 3 months each time. Before and six months after treatment, patients and surgeons used the Patient and Observer Scar Assessment Scale (POSAS) to evaluate each aspect of scar. The total score for each item was 1-10 points. According to the total POSAS scores of patients and doctors, the degree of improvement of depressed scars was classified and evaluated. Grade 1: invalid, total score reduction ≤ 5 points; grade 2: mildly effective, total score reduction> 5 points and ≤ 10 points; grade 3, effective, total score reduction> 10 points and ≤ 20 points; grade 4, markedly effective, total score reduction> 20 points. Using SPSS 23.0 to do t-test on the data before and after treatment. P<0.05 indicates that the difference is statistically significant. Results:A total of 27 patients were enrolled, including 16 males and 11 females, aged (29.1±7.9) years old. Before and after six months of treatment, the difference between the scores of the patients on all indicators of scar was statistically significant ( P<0.05); the difference between the doctor’s scores was statistically significant ( P<0.01). The total scores of post-total POSAS were (43.7±10.8) and (23.3±3.2) points respectively, the difference was statistically significant ( P<0.01). After six months of treatment, the degree of pain, itching, color, hardness, thickness (depression), elasticity, tension of the depressed scar were significantly improved. Among them, there was 1 case of second-level improvement, 14 cases of third-level improvement, and 12 cases of fourth-level improvement. The total effective rate reached 96.3% (26/27). None of the 27 patients had lumps or cord-like indurations on the filling parts of the face. No embolism, infection, hematoma and other complications occurred after nanofat transplantation in all patients. Skin flushing, burning, edema, pain and other symptoms occurred early after the fractional CO 2 laser treatment, and no hyperpigmentation occurred in the 6-month follow-up after a treatment cycle. Conclusions:The combination of nanofat transplantation technology and carbon dioxide fractional CO 2 laser can produce a significant effect on improving depressed scar, reducing treatment period, rendering a simple operation with few complications.