Starting with the concepts of basic dataset and basic dataset of nursing management information of hospital of Tradtional Chinese Medicine (TCM),the paper introduces the research purpose,construction principle,construction method,construction contents and system framework of the basic dataset standard system of nursing management information of hospital of TCM,in order to lay a foundation for achieving nursing management data resource sharing and exchange of hospital of TCM.

10.3969/j.issn.2095-4344.2013.25.012

BACKGROUND:A novel galactosylated alginate-chitosan oligomer microcapsule has been prepared successful y. There is no report on hepatocytes encapsulated into the microcapsule prepared with chitosan oligomer and alginate sodium.
OBJECTIVE:To study the structure and properties of the previous novel galactosylated alginate-chitosan oligomer microcapsule, and then explore the morphology and function expression of the hepatocytes encapsulated.
METHODS:The membrane structure and thickness of the microcapsule (containing 50%or 30%galactosylated alginate) were observed using a laser confocal microscopy. Mechanical property was determined by mechanical rupture rate. Permeability was evaluated by release profile of fluoresceine isothiocyanate-labeled human serum albumin and immunoglobulin G. The morphology of hepatocytes in the microcapsule was observed using an inverted phase contrast microscopy. Function expression of the hepatocytes included albumin secretion and urea synthesis.
RESULTS AND CONCLUSION:The microcapsule had an asymmetry structure, with dense inner and loosened outer surfaces. With the increase of the galactosylated alginate, the membrane became loose, which indicated the negative charge on the alginate molecular chains was weakened after introduction of galactose, and thus electrostatic complex was affected. Mechanical property was correlated with both membrane structure and thickness. With the increase of the galactosylated alginate, the membrane structure became loose and the thickness was decreased, resulting in poor mechanical properties. The permeability was dependent mainly on the pore size of the skin layer of the membrane other than the loose sublayer. The prepared microcapsule can selectively pass through the human serum albumin and cut off immunoglobulin G, indicating skin pore size between human serum albumin and immunoglobulin G. The hepatocytes can form sphere assemble in the galactosylated alginate-chitosan oligomer microcapsule and exhibit improved albumin secretion and urea synthesis compared to the control in the alginate-chitosan oligomer microcapsule.

BACKGROUND: Ions doping is an important method for the modification of bioceramic.OBJECTIVE: To evaluate a novel co-substituted bioceramic scaffolds as bone repair material.METHODS: The microstructure and crystallization of the scaffolds were detected by scanning electron microscope and X-ray diffraction. Compression strength test,degradation test and cell culture experiment were assumed to evaluate the properties of KSCPP in vitro. After a short period of muscle pouches implantation,the performance of KSCPP in vivo was evaluated.RESULTS AND CONCLUSION: The results show that KSCPP scaffold has a higher compressive strength and degradation rate. Moreover,the MTT assay and implantation test reveal that the KSCPP scaffold exhibits lower cytotoxicity and better tissue biocompatibility than CPP and HA. The study proved the great potential of KSCPP in bone repair applications.

Calcium polyphosphate (CPP) is a new type of degradable material for bone repair, yet it is fragile and is not so controllable in regard to degradation. For increasing biological activity and close proximity to natural bone structure, in this experiment, we chose chitosan (CS) and its derivative carboxymethyl chitosan (CMC) as the extracellular matrix structure for the organic phase. Aldehyde sodium alginate (ADA) was used as natural cross-linker. The binary (CPP/CMC) and ternary (CPP/CMC/CS) composite scaffolds were prepared by the "multiple composite-cross-linking method". The degradation laws of the two materials were investigated through the weight loss of scaffolds, the pH value of degradation solution, the compressive strength and the surface morphology characterization. The results showed that the composite scaffolds had good interface and the compressive strength increased greatly, but the organic phase of dual-phase composite scaffolds degraded quickly, while degradation controllability and mechanical properties of ternary composite scaffold were significantly improved. All the above findings show that the method of ternary complex scaffold preparation is useful for the design and preparation of bone tissue engineering materials.
Absorbable Implants ; Biocompatible Materials ; chemical synthesis ; chemistry ; Bone Cements ; chemical synthesis ; chemistry ; Calcium Phosphates ; chemistry ; Chitosan ; chemistry ; Humans ; Tissue Engineering ; Tissue Scaffolds ; chemistry

Tissue engineered meniscus provides a novel approach for the treatment of severe meniscus injury. The researches on scaffold for tissue engineered meniscus is an essential element. The researches on scaffolds for tissue engineered meniscus, including natural biomaterials and synthetic polymer, have made great advances in recent years. At present, collagen meniscus implants have been used in clinical practice. As hydrogel is characterized by having high water content and good biocompatibility, being similar to extracellular matrix in structure, and being able to provide access to microsurgery, it has become the hotspot field in recent years. Meanwhile, the combinatorial scaffold material advantages of biological composite tissue engineered scaffolds also illuminate the key point of research on meniscal scaffolds. This paper reviews the recent progress in betterment of the scaffolds so as to provide a theoretical and practical basis for further researches of tissue engineered meniscus.
Animals ; Biocompatible Materials ; Cells, Cultured ; Chondrocytes ; cytology ; Humans ; Hydrogels ; Menisci, Tibial ; cytology ; surgery ; Regeneration ; physiology ; Tibial Meniscus Injuries ; Tissue Engineering ; Tissue Scaffolds

BACKGROUND: Strontium-doped calcium polyphosphate (SCPP) is a new type of bone repair materials with good biocompatibility and controlled degradation. The preliminary studies of our group indicate their role in promoting angiogenesis,but its mechanism is unclear.OBJECTIVE: By co-culturing osteoblasts ROS17/2.8 with SCPP in vitro to observe cell proliferation and the secretion of vascular endothelial growth factor (VEGF).DESIGN, TIME AND SETTING: A contrast study was performed at the Laboratory of Tissue Engineering of Sichuan University from October 2008 to June 2009.MATERIALS: A series of calcium polyphosphate (CPP) respectively containing 0%, 1 %, 2%, 5%, 8%, and 10% Sr~(2+) were prepared. ROS17/2.8 osteoblastic cell strain was provided by Laboratory of Transplantation Immunity and Transplantation Engineering, West China Hospital, Sichuan University.METHODS: ①Preparation of cell scaffold complexes: The materials were placed in 24-well plates, then 300 μL cell suspension with a concentration of 2×10~7 cells/Lwas inoculated into each hole. These complexes were cultured for 14 days and the liquid was changed every two days. ②These complexes were measured by MTT assay to observe the proliferation of osteoblasts on the 1~(st), 3~(rd), 5~(th), 7~(th), 10~(th) and 14~(th) days, respectively. ③ The centrifugal supernatant of the complex cultured for seven days was measured by ELISA assay to check the secretion of VEGF.MAIN OUTCOME MEASURES: The proliferation of osteoblastic cells on SCPP and CPP was observed. The amount of VEGF protein secreting from osteoblastic cells was detected.RESULTS: The results of MTT showed that, compared with the CPP group, SCPP groups could promote the proliferation of osteoblasts, and 8% SCPP group was the best; ELISA results showed that, compared with the CPP group, SCPP groups could increase the amount of VEGF protein secretion, of which the promoting role of 8% SCPP was the most obvious (P < 0.05).CONCLUSION: When cultured with osteoblasts, SCPP can promote cell proliferation, and can significantly increase the secretion of VEGF; moreover, 8% SCPP is the best, which reveals a certain mechanism of its promoting angiogenesis.

BACKGROUND: Strontium-doped calcium polyphosphate (SCPP), as a new repairing material, has been demonstrated to have favorable biocompatibility and biodegradability and some effects on promoting self-angiogenesis. However, the mechanism remains still unknown. OBJECTIVE: Endothelial cells were cultured with SCPP scaffolds in vitro, as well as the cell proliferation and angiogenic factor matrix metalloproteinase-2 (MMP-2) secretion were observed. DESIGN,TIME AND SETTING: A contrast study was performed at the Laboratory of Tissue Engineering of Sichuan University from September 2008 to April 2009. MATERIALS: A series of calcium polyphosphate (CPP) respectively containing 1 %, 2%, 5%, 8%, and 10% Sr~(2+) were prepared.METHODS: ① Materials were plated on 24-well culture plate,and endothelial cell suspension (300 μL) were seeded on 24-well culture plate at the concentration of 3×10~7/L and cultured with 200 uL RPMI1640 culture media. Endothelial cell proliferation was observed using MTT method at days 1,3,5, and 7 after culture. ② CPP and 8% SCPP were plated on 24-well culture plate, and endothelial cell suspension (300 uL) was then incubated in 24-well culture plate at the concentration of 1x10~8/L and cultured with 600 uL RPMI1640 culture media. The morphology of endothelial cells was observed by scanning electron microscopy (SEM) at day 5 after culture.③ Endothelial cells were co-cultured with SCPP of various Sr~(2+) contents for 5 days. After confluence, cells were centrifuged to obtain supernatant. Angiogenic factor MMP-2 secretion was evaluated by ELISA assay.MAIN OUTCOME MEASURES: The proliferation and morphology of endothelial cells on SCPP and CPP were observed. The amount of endothelial cells-derived MMP-2 protein secretion was detected. RESULTS: MTT method demonstrated that the proliferation of endothelial cells on the 8% SCPP scaffold showed a higher level compared to CPP, and other SCPP groups. Scanning electron microscope results suggested that endothelial cells on 8% SCPP had a better growth status and biological activity. ELISA results indicated that angiogenic factor MMP-2 expression on the SCPP was promoted compared with that of CPP, and 8% SCPP showed the highest expression (P < 0.05). CONCLUSION: SCPP has good compatibility with endothelial cells,promoting angiogenesis and enhancing the angiogenic factor MMP-2 expression.

A series of novel biodegradable and bionic functional polymers, PLLA-PC-PLLA, were synthesized using L-lactide ring-opening polymerization by L-a-Glycerophosphorylcholine (PC) from nature source. The hydrophilicity of the polymers was investigated. The results made known that, as PC group was brought into the backbone of PLLA, PLLA-PC-PLLA gained much better hydrophilicity than did PLLA, and polar phosphatidylcholines probably transferred to the sample surface in aqueous environment. The relative growth ratios of ECV304 cells to the lixivium of all PLLA-PC-PLLA were higher than 84% in 5 d culture. The cells adhesion of ECV304 on the films of PLLA-PC-PLLA lagged as compared to that on PLLA, but they could proliferate and cover the films in total. The difference between PLLA-PC-PLLA and PLLA was due to the existence of PC group. Thus, PLLA-PC-PLLA, the same as PLLA, are not cytotoxic, and ECV304 can attach and proliferate on them. PLLA-PC-PLLA have potential applications in the fields of tissue engineering and drug delivery system.
Cell Adhesion ; physiology ; Drug Carriers ; Endothelial Cells ; cytology ; Humans ; Hydrophobic and Hydrophilic Interactions ; Lactic Acid ; chemistry ; pharmacology ; Phosphorylcholine ; chemistry ; pharmacology ; Polyesters ; Polymers ; chemistry ; pharmacology ; Surface Properties ; Tissue Scaffolds ; Umbilical Veins ; cytology

This study was undertaken to elucidate the degradation regularity of calcium polyphosphate (CPP) scaffolds with different preparation parameters. CPP scaffolds with different main crystalline phases were prepared by controlling the particle size of the calcining stuff and the calcining heat. Specimens were soaked into Tris-buffer solution and simulated body fluid (SBF) for 60 days. Results show: alpha-CPP degrades faster than does beta-CPP, and beta-CPP degrades faster than does gamma-CPP; the lower the sinter temperature, the better the degradation of CPP morever, the degradation rate of CPP is inversely proportional to the original particle size. These data suggest that crystal type, sinter temperature and particle size influence the degradation rate of CPP markedly.
Absorbable Implants ; Biocompatible Materials ; chemistry ; Bone Substitutes ; chemistry ; Calcium ; chemistry ; Calcium Phosphates ; chemistry ; Ceramics ; chemistry ; Polymers ; chemistry ; Polyphosphates ; chemistry ; Tissue Scaffolds ; chemistry