PURPOSE: Advanced hepatocellular carcinoma (HCC) is associated with various clinical conditions including major vessel invasion, metastasis, and poor performance status. The aim of this study was to establish a prognostic scoring system and to propose a sub-classification of the Barcelona-Clinic Liver Cancer (BCLC) stage C. MATERIALS AND METHODS: This retrospective study included consecutive patients who received sorafenib for BCLC stage C HCC at a single tertiary hospital in Korea. A Cox proportional hazard model was used to develop a scoring system, and internal validationwas performed by a 5-fold cross-validation. The performance of the model in predicting risk was assessed by the area under the curve and the Hosmer-Lemeshow test. RESULTS: A total of 612 BCLC stage C HCC patients were sub- classified into strata depending on their performance status. Five independent prognostic factors (Child-Pugh score, α-fetoprotein, tumor type, extrahepatic metastasis, and portal vein invasion) were identified and used in the prognostic scoring system. This scoring system showed good discrimination (area under the receiver operating characteristic curve, 0.734 to 0.818) and calibration functions (both p < 0.05 by the Hosmer-Lemeshow test at 1 month and 12 months, respectively). The differences in survival among the different risk groups classified by the total score were significant (p < 0.001 by the log-rank test in both the Eastern Cooperative Oncology Group 0 and 1 strata). CONCLUSION: The heterogeneity of patientswith BCLC stage C HCC requires sub-classification of advanced HCC. A prognostic scoring system with five independent factors is useful in predicting the survival of patients with BCLC stage C HCC.
Calibration ; Carcinoma, Hepatocellular* ; Cohort Studies* ; Discrimination (Psychology) ; Humans ; Korea ; Liver Neoplasms ; Neoplasm Metastasis ; Population Characteristics ; Portal Vein ; Prognosis ; Proportional Hazards Models ; Retrospective Studies ; ROC Curve ; Tertiary Care Centers

This study investigated changes in certain blood parameters in calves and pigs after foot-and-mouth disease (FMD) vaccination. In this study, five calves and five pigs were selected from groups of 10 calves and pigs, respectively, and were vaccinated with an FMD vaccine. The remaining animals formed two non-treatment control groups. Blood samples were collected from all animals on the 1st, 3rd, 5th, and 7th days post-vaccination. In the FMD-vaccinated calves and pigs on day 7 post-vaccination, white blood cell counts, blood urea nitrogen levels, and alanine aminotransferase and aspartate aminotransferase activities were higher than those in the respective controls. The present data suggested that the certain hemato-biochemical parameters on cattle and pigs were meaningfully changed between before and after FMD vaccination.
Alanine Transaminase ; Animals ; Aspartate Aminotransferases ; Blood Urea Nitrogen ; Cattle ; Foot-and-Mouth Disease* ; Hematologic Tests ; Leukocyte Count ; Swine* ; Vaccination*

This study was conducted to analyze penicillin G (PEG), streptomycin (STR) and neomycin (NEO) residues in milk of healthy lactating cows. Milk samples were collected from all four quarters of 12 dairy cows 2–7 days after intramammary infusions of an ointment containing PEG, STR and NEO once (n = 4; group I) or twice (n = 4, group II) daily. Ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry was used to determine the antibiotic residues in the samples. The correlation coefficient (r 2) of the calibration curves for all antibiotics was > 0.999 and the limits of detection and quantification were 0.002–0.005 µg/mL and 0.007–0.02 µg/mL, respectively. Recovery rates were ranged from 75.5 to 92.3%. In group I, PEG, STR and NEO residues were detected in milk at 2, 3 and 2 days post-treatment, respectively, which were below the maximum residue limit (MRL). In group II, PEG, STR and NEO residues were detected in milk at 2, 3 and 3 days post-treatment, respectively, which were bellow the MRL. These results suggest that a 3-day for milk withdrawal period after the ointment treatment might be sufficient for reduction of the antibiotic residues below the MRL.

This study investigated the effects of LactoPlanta(R) (Lactobacillus plantarum (L. plantarum), 2.0 x 10(9) colony forming units (CFU)/kg) on reduction of noxious gas emission in pig houses as well as improvement of carcass weight and quality in finishing pigs. A total of 850 finishing pigs were assigned to four treatment groups: control (CON, basal diet) (n=190), LP-0.1, 0.1% LactoPlanta(R) (n=210), LP-0.2, 0.2% LactoPlanta(R) (n=230), and LP-0.4, 0.4% LactoPlanta(R) (n=220). Ammonia and hydrogen sulfide concentrations were significantly reduced in all treatment groups compared to CON. Mercaptan contents and carcass weights of LP-0.2 and LP-0.4 were significantly decreased compared to CON, whereas there were no significant differences between LP-0.1 and CON. Carcass weight of LP-0.1 was slightly higher than that of CON, but there was no significant difference. However, carcass weights of LP-0.2 and LP-0.4 were significantly higher than that of CON (P<0.05). The prevalence of grade A carcasses in groups administered with L. plantarum (46.7~63.3%) was higher than that in CON (43.3%) and increased in a dose-dependent manner. Based on the results of this study, L. plantarum could be an effective candidate to reduce noxious gas emissions in finishing pig houses as well as improve carcass weight and quality in finishing pigs.
Ammonia ; Hydrogen Sulfide ; Lactobacillus plantarum* ; Prevalence ; Stem Cells ; Swine* ; Weights and Measures

Partial or complete absence of the posterior arch of the atlas is a well-documented anomaly but a relatively rare condition. This condition is usually asymptomatic so most are diagnosed incidentally. There have been a few documented cases of congenital defects of the posterior arch of the atlas combined with atlantoaxial subluxation. We report a very rare case of congenital anomaly of the atlas combined with atlantoaxial subluxation, that can be misdiagnosed as posterior arch fracture.
Adult ; Cervical Atlas/*abnormalities ; Diagnosis, Differential ; Female ; Humans ; Spinal Diseases/*diagnosis

This study evaluated the antibacterial effects of a mixture of Sophorae radix and Glycyrrhiza uralensis Fischer (1 : 1) ethanol extracts (SGE) on mice infected with Streptococcus (S.) pyogenes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of SGE necessary for antibacterial effects against S. pyogenes were 20 microg/mL. Based on the time-kill curves for S. pyogenes, SGE was effective at 4x MIC after 16 h. On Day 12 after challenge, the survival rate of mice treated with 2.0 mg/kg SGE was 60%. In conclusion, SGE had potent in vitro and in vivo antibacterial activities against S. pyogenes.
Animals ; Complex Mixtures ; Ethanol* ; Glycyrrhiza uralensis* ; Mice* ; Microbial Sensitivity Tests ; Plants, Medicinal ; Sophora* ; Streptococcus ; Streptococcus pyogenes* ; Survival Rate ; Treatment Outcome

This study evaluated the antibacterial effects of a mixture of Sophorae radix and Glycyrrhiza uralensis Fischer (1 : 1) ethanol extracts (SGE) on mice infected with Streptococcus (S.) pyogenes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of SGE necessary for antibacterial effects against S. pyogenes were 20 microg/mL. Based on the time-kill curves for S. pyogenes, SGE was effective at 4x MIC after 16 h. On Day 12 after challenge, the survival rate of mice treated with 2.0 mg/kg SGE was 60%. In conclusion, SGE had potent in vitro and in vivo antibacterial activities against S. pyogenes.
Animals ; Complex Mixtures ; Ethanol* ; Glycyrrhiza uralensis* ; Mice* ; Microbial Sensitivity Tests ; Plants, Medicinal ; Sophora* ; Streptococcus ; Streptococcus pyogenes* ; Survival Rate ; Treatment Outcome

Angular deformities of the distal femur occur in congenital diseases or due to acquired causes, such as malunion after a fracture of the distal femur. Angular deformities of the lower extremities affect the mechanical axis, causing changes in the weight pressure on the articular surface. As a result, angular deformities quicken the progression of osteoarthritis. Therefore, correction of deformities should be performed to prevent the progression of osteoarthritis. Distal femoral osteotomy is one of the methods to correct angular deformities in unicompartmental osteoarthritis. However, femoral supracondylar dome osteotomy with retrograde intramedullary nailing in the distal femur with a varus deformity has been rarely reported. Herein, we describe a technique for femoral supracondylar dome osteotomy with retrograde intramedullary nailing in a varus deformity after a pathologic fracture of giant cell tumor in the distal femur with a review of the relevant literature.
Axis, Cervical Vertebra ; Congenital Abnormalities ; Femur ; Fracture Fixation, Intramedullary ; Fractures, Spontaneous ; Giant Cell Tumors ; Giant Cells ; Lower Extremity ; Methods ; Osteoarthritis ; Osteotomy

OBJECTIVE: This study examined the prevalence and correlates of depressive symptoms in North Korean defectors who have been living in South Korea for more than one year. METHODS: We used questionnaires developed by the authors to collect sociodemographic data in addition to the Center for Epidemiologic Studies Depression Scale (CES-D), the Psychosocial Well-being Index to measure stress, and a social support scale. A total of 367 subjects were included in this study. RESULTS: The results showed that 30.5% of the men and 34.7% of the women reported depressive symptoms, and 33.1% of the men and 36.1% of the women exhibited signs of severe distress. Correlates of depressive symptoms were lack of occupation [odds ratio (OR)=2.198, 95% confidence interval (CI), 1.247-3.873], having escaped without family (OR=1.725, 95% CI, 1.006-2.959), and a poor subjective sense of health status (OR=3.111, 95% CI, 1.591-6.085). CONCLUSION: Continuing vocational training and career management, psychological support programs, and intensive physical health services are needed to improve the mental health of this population.
Depression ; Epidemiologic Studies ; Female ; Health Services ; Humans ; Male ; Mental Health ; Occupations ; Prevalence ; Surveys and Questionnaires ; Republic of Korea ; United Nations

Human embryonic stem (ES) cells can be induced to differentiate into hematopoietic precursor cells via two methods: the formation of embryoid bodies (EBs) and co-culture with mouse bone marrow (BM) stromal cells. In this study, the above two methods have been combined by co-culture of human ES-cell-derived EBs with human BM stromal cells. The efficacy of this method was compared with that using EB formation alone. The undifferentiated human ES cell line SNUhES3 was allowed to form EBs for two days, then EBs were induced to differentiate in the presence of a different serum concentration (EB and EB/high FBS group), or co- cultured with human BM stromal cells (EB/BM co-culture group). Flow cytometry and hematopoietic colony-forming assays were used to assess hematopoietic differentiation in the three groups. While no significant increase of CD34+/CD45- or CD34+/CD38- cells was noted in the three groups on days 3 and 5, the percentage of CD34+/CD45- cells and CD34+/ CD38- cells was significantly higher in the EB/BM co-culture group than in the EB and EB/high FBS groups on day 10. The number of colony-forming cells (CFCs) was increased in the EB/BM co-culture group on days 7 and 10, implying a possible role for human BM stromal cells in supporting hematopoietic differentiation from human ES cell-derived EBs. These results demonstrate that co-culture of human ES-cell-derived EBs with human BM stromal cells might lead to more efficient hematopoietic differentiation from human ES cells cultured alone. Further study is warranted to evaluate the underlying mechanism.
Stromal Cells/physiology ; Stem Cells/*cytology ; Humans ; Hematopoietic Stem Cells/*cytology ; Embryo/*cytology ; Coculture Techniques ; Cells, Cultured ; *Cell Differentiation ; Bone Marrow Cells/*cytology ; Antigens, CD45/analysis ; Antigens, CD38/analysis ; Antigens, CD34/analysis