Objective To observe the clinical efficacy of Thunder-fire moxibustion combined with traditional Chinese and western medical treatments and nursing interventions for peripheral facial paralysis.Methods Fifty inpatients and outpatients diagnosed with peripheral facial paralysis at Shanxi University of Chinese Medicine Acupuncture Hospital from January 2017 to October 2018 were selected as the research subjects and randomly divided into a control group and a treatment group,with 25 cases in each group.The control group received conventional treatment and nursing for facial paralysis,while the treatment group received Thunder-fire moxibustion combined with effective nursing interventions on the basis of the treatment and nursing in the control group.The treatment and nursing were conducted for 5 consecutive days as one course,with a total of 2 courses:①Thunder-fire moxibustion:ignite the Thunder-fire moxibustion,keeping the moxa stick 2-3 cm away from the affected skin,and apply both transverse and longitudinal moxibustion methods.Press the skin at the moxibustion site with your finger every 10 times the moxa stick is shaken,and then use the circular method,selecting the acupoints Dicang,Jiache,Quanliao,and Yangbai,with a distance of 1.5 cm from the acupoints,moxibustion for each point for 8-10 minutes;②Acupuncture:select the affected side acupoints Yangbai,Quanliao,Taiyang,Dicang,Jiache,Jingming,Yingxiang,Sizhukong,Yuyao,and Touwei,and the contralateral Hegu and bilateral Zusanli.Retain the needle for 30 minutes after obtaining Qi,once daily;③Traditional Chinese medicine steaming:use the hospital's self-prescribed formula(composed of Cinnamomum cassia,Poria cocos,Carthamus tinctorius,Carica papaya,Angelica sinensis,Paeonia lactiflora,Spatholobus suberectus,and Saposhnikovia divaricata,each 15 g),decoct and pour the juice into the medicine addition area.The steaming temperature is 40-45℃,with the steaming nozzle 25-30 cm away from the affected side of the face,steaming for 20 minutes,once daily;④ Medication treatment:in the early stage,antiviral drugs such as acyclovir were given,and traditional Chinese medicine was mainly used to eliminate wind and phlegm,and to dredge meridians;⑤ Nursing intervention:during thunder-fire moxibustion,the flame should not touch the skin to prevent burns;combined acupoint pressing with massage techniques;paid attention to observation and inquire about the patient's feelings during moxibustion;after moxibustion,informed the patient that the blood vessels in the head and face were in a state of dilation,and that it was normal for the facial skin to turn red and itchy.Instructed patients to rest,exercised appropriately,avoided wind and cold,refrain from raw,cold,greasy foods,and avoid tobacco,alcohol,and spices,and taught them self-training exercises.The differences in efficacy scores,clinical efficacy,patient compliance rates,and satisfaction between the two groups were compared,and any adverse reactions were observed.Results After 2 courses of treatment and nursing,the clinical efficacy scores of both groups were significantly lower than those after 1 course of treatment and nursing,and the degree of reduction in the treatment group was more significant than that in the control group(1.12±0.88 vs.1.79±0.76,P<0.05).The total effective rate in the treatment group was higher than that in the control group[100.0%(25/25)vs.96.0%(24/25)],but the difference was not statistically significant(P>0.05),and the compliance rate was significantly higher than that in the control group[100.0%(25/25)vs.20.0%(5/25),P<0.05],with a satisfaction rate of 100.0%for both groups.No serious adverse events occurred in either group.Conclusions Thunder-fire moxibustion has sufficient medicinal strength,strong efficacy,and significant permeation effects,with simple operation.Combined with traditional Chinese and western medical treatments and nursing interventions,it shows satisfactory efficacy in the treatment of peripheral facial paralysis,with a short course of treatment,high patient compliance,satisfaction,and no adverse reactions.

[Abstract] Objective: To investigate the effect of lncRNA SNHG15 targeting miR-153 on cell viability and apoptosis of breast cancer cells and its apoptotic mechanism. Methods:The expression of SNHG15 in breast cancer cell lines(MDA-MB-231, BT-549 and MCF-7) were detected by Real-time fluorescent quantitative PCR (qPCR). MDA-MB-231 cells were divided into control (Ctrl) group, si-NC group, si-SNHG15 group, si-SNHG15+anti-NC group and si-SNHG15+anti-miR-153 group. Cell viability and apoptosis rate were detected by MTT and Flow cytometry, respectively. The targeting relationship between SNHG15 and miR-153 was verified by Dual luciferase report gene system. Mitochondrial membrane potential fluorescent probe (JC-1) staining method was used to detect cell mitochondrial membrane potential. The expressions of mitochondrial apoptosis-related proteins (Bcl-2, Bax, caspase3, cleaved caspase3 [c-caspase3] and Cyt-C）were detected by Western blotting. Results: The expression of SNHG15 in breast cancer cells was significantly higher than that in human normal mammary epithelial MCF10A cells (P<0.01). There was a targeting relationship between SNHG15 and miR-153. Compared with the control group, the cell viability and mitochondrial membrane potential of MDA-MB-231 cells in si-SNHG15 group were decreased, while apoptosis rate was increased (all P<0.01); the expressions of Bcl-2 and caspase3 were decreased while expressions of Bax, c-caspase3 and Cyt-C were increased (all P<0.01). However, co-transfection of si-SNHG15 and anti-miR-153 significantly attenuated the effects of si-SNHG15 on cell viability, apoptosis, mitochondrial membrane potential and expressions of Bcl-2, Bax, caspase3, c-caspase3 and Cyt-C (all P<0.01). Conclusion: lncRNA SNHG15 can target miR-153 to induce apoptosis of MDA-MB-231 cells, and the mechanism may be related to the regulation of apoptosis of mitochondrial pathway.

ObjectiveTo evaluate therapeutic effect and the possible mechanism of smecta on paraqual plasma concentrations and multiorgans injury induced by paraquat intoxication in rats. Methods A total of 76 healthy adult SD rats were randomly ( random number) divided into group A (control group n =6),group B ( poisoned group n =30 ),group C (smecta-treated group n=30).Rats in groups B and C were treated intragastrically with PQ at 50 mg/kg,the rats in the group C were given with smecta at 50 mg/kg,while the rats in the other two groups were only intragastrically adminstered with saline.Live rats in groups B and C were sacrificed at 2,6,24,48,72 h after administration of PQ for the determination of paraquat plasma concentrations and for HE staining of lung,stomach and jejunum.The rats were executed at the end of trial by the same way in group A.All measurement data were expressed as means + standard deviation ((x) ±s).The data of pathological score were compared with Independent-samples T test and the data of PQ concentration compared with analysis of variance (ANOVA) followed by LSD-t multiple comparison test.P-values of less than 0.05 were considered statistically significant.ResultsThe paraquat plasma concentration ( ng/ml ) was 440.314 ± 49.776 to 4320.6150 ± 413.947.There were different pathological changes of lung,stomach and jejunum in group B. Lung injuries gradually deteriorated,congestion,edema,leukocyte infiltration,incrassated septa and lung consolidation were observed.The pathological changes were obvious such as abruption of mucosa,hyperemic gastric mucosa and leukocyte infiltration in stomach.Haemorrhage of jejunum mucosa,abruption of villus,gland damage and inflammatory cell infiltration were found. Compared with group B,all the pathological changes mentioned above were obviously alleviated in group C ( P ＜ 0.05 ),and the concentrations reduced ( P ＜ 0.01 ).Conclusions Smecta reduced paraquat plasma concentrations and alleviated pathologic injury of rats with PQ poisoning.

Objective To explore the relationship between the somatosensory evoked potential (SEP) diversification during operation and the short term outcome of ossification of posterior longitudinal ligament (OPLL) of cervical spine. Methods 70 OPLL inpatients from February 2008 to February 2011were included in this study. All patients received anterior cervical operation. According to the diversification of the SEP during surgery, the patients were divided into 2 groups. The preoperative and postoperative JOA scores of the OPLL patients were collected. Results There were 35 patients in the SEP no change group and 30 in SEP improved group. There was no difference in the preoperative JOA scores between them (P>0.05), but was significantly different in the postoperative JOA scores (P>0.05). Conclusion There is some relationship between the SEP diversification during operation and the short term outcome of OPLL of cervical spine.

Objective To investigate the balance function of the knee osteoarthritis (KOA) patients and the clinical value about static fallindex test and dynamic fall index test for the patients with knee osteoarthritis. Methods The osteoarthritis patients without knee pain (controlgroup, n=30) were tested with postural stability (PS), limits of stability (LOS), dynamic fall index (DFI) and static fall index (SFI). Theosteoarthritis patients with knee pain (treatment group, n=30) accepted 10 times of diclofenac diethylamine emulge imported by pulsed ultrasoundand 6 times of massage therapy. They were also tested with above four balance tests and Western Ontario and McMaster UniversitiesOsteoarthritis Index (WOMAC) before and after treatments. Results There was significant difference in overall of PS, anterior/posterior indexand medial lateral index (P<0.05) between the control group and the treatment group before treatments, and there was very significantdifference in time to complete LOS, overall of LOS and DFI (P<0.01); but there was no significant difference in SFI (P<0.05). There wassignificant difference in WOMAC scores, overall of PS, anterior/posterior index, time to complete LOS, overall of LOS and DFI (P<0.05) inthe treatment group before and after treatments, but there was no significant difference in medial lateral index and SFI (P<0.05). ConclusionCompared with osteoarthritis patients without knee pain, KOA patients with knee pain are in poorer balance function and greater fall risk.After effective treatments, the balance function and fall risk of the KOA patients may significantly improve. Compared with SFI test, theDFI test is more sensitive to the improvement of balance function of the KOA patients.

ObjectiveTo examine the test-retest reliability and measurement error in using a Jamar dynamometer to determine grip strength for elder men. Methods40 elder men with a mean age of 82 years old volunteered to participate in this study. The grip strength was administered twice using a Jamar dynamometer by specially trained testers; subjects were re-tested at the same time of day a week later by the same testers. ResultsThe infraclass correlation coefficient（ICC） was 0.99. The standard error of measurement (SEM and SEM%) were 1.18～1.69 and 4.11%～6.41%. The smallest real difference (SRD and SRD%) were 3.27～4.68 and 11.39%～17.75%. The Bland-Altman analysis revealed no systematic errors between test and retest. ConclusionThe Jamar dynamometer is reliable and with acceptable measurement errors to determine grip strength for elder men.

ObjectiveTo investigate the age-related changes in grip strength of the elderly men and compare with the norms of overseas. Methods112 subjects were divided into six groups: 65～69 years old, 70～74 years old, 75～79 years old, 80～84 years old, 85～89 years old, and 90～95 years old. The grip strength was measured with Jamar dynamometer. ResultsThe grip strength of both sides were significantly moderate negative correlated to age (r=-0.681 and -0.68, P<0.001) and stepwise regression analysis showed the age was important clinical factor to consider in determining grip strength in the elderly men （R2=0.468, F=47.951, P＜0.001). The grip strength was declined significantly during the 75～79 and 85～89 years old. ConclusionGrip strength had declined in the elderly men and these losses were significantly related to age. The highest loss in men was in the age over 75. The norms of grip strength overseas were not applicable to Chinese older adults.

ObjectiveTo determine the test-retest reliability of isometric muscle strength measurement for the lower limb with functional squat system （FSS） in elderly people. Methods20 elderly participants were measured the isometric muscle strength in 5 s of each lower limb with FSS, and retested a week later. The maximal voluntary contraction and the average strength of each lower limb were recorded. ResultsThe intraclass correlation coefficient were 0.77～0.88 (P<0.01). ConclusionIsometric muscle strength measurement of the lower limb with FSS shows acceptable test-retest reliability in elderly.

ObjectiveTo investigate the relationship of lower extremity muscle strength and the pain or physical function in patients with knee osteoarthritis. Methods25 patients with knee osteoarthritis diagnosed with have radiological and clinical data were assessed with Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and isokinetic muscle strength testing. ResultsThe lower extremity extensor muscle strength correlated with WOMAC pain scores, WOMAC activity of daily life scores, and WOMAC total scores （P<0.05）. ConclusionThe lower extremity extensor muscle strength correlated with the pain and physical function in patients with knee osteoarthritis.

ObjectiveTo determine the value of the five-times sit-to-stand test (FTSST) on assessment of physical performance for older people.Methods65 volunteers participated in this study. 21 of them were evaluated the FTSST twice by the same raters within one week. Test-retest reliability assessed using intraclass correlation coefficients (ICC). 65 volunteers were tested for the FTSST, the Timed "up & go" test(TUGT) and the strength of lower limbs. The Pearson's correlation (r) and stepwise regression analysis was used to examine the relationship between these variables and the FTSST time.ResultsThe FTSST showed good test-retest reliability(ICC=0-91, 95%CI:0-79～0-96). The TUGT, the strength of lower limbs and age were significantly related to the FTSST time (P<0-05). The strength of lower limbs was important clinical factor to consider in determining the FTSST time.ConclusionThe FTSST showed good test-retest reliability and can reflect the physical functional status of older people.