Objective:To explore the impact of sleep quality on depression symptoms in perimenopausal women in urban communities.Methods:Using the convenient sampling method, a total of 2 300 perimenopausal women were selected as the research subjects from communities in four cities of Ya'an, Chengdu, Leshan and Deyang in Sichuan Province in March 2019. General Information Questionnaire, Patients Health Questionnaire-9 Items (PHQ-9) and Pittsburgh Sleep Quality Index (PSQI) were used to investigate the perimenopausal women. A total of 2 300 questionnaires were sent out in this study, and 2 300 were effectively received, with the effective recovery of 100% (2 300/2 300) .Results:The detection rate of depressive symptoms in perimenopausal women was 12.5% (288/2 300). The dominance analysis showed that sleep quality was the most important factor affecting depressive symptoms, followed by life events, marital status, chronic illness, per capita monthly household income and education level.Conclusions:Sleep quality is the most important factor affecting the depressive symptoms of perimenopausal women. It should be emphasized to improve the level of depression in perimenopausal women.

Objective:To analyze the clinical characteristics, diagnosis and treatment strategies of cryptococcus neoformans infection in children, and to explore the application value of PCR and high-throughput gene analysis in the diagnosis and treatment of cryptococcus neoformans infection.Methods:The clinical data of children with cryptococcus neoformans infection were retrospectively analyzed, who were hospitalized in Shanghai Children′s Medical Center from July 1998 to December 2018, including epidemiological characteristics (age, gender, underlying disease, and contact history, etc.), clinical manifestations, laboratory tests, imaging studies, pathogen detection methods, and treatment and prognosis.Results:A total of ten children were enrolled in the survey, including five males and five females, and the median age was 6.28(4.08, 12.02) years; four cases had a history of poultry/soil/corrosion exposure; seven cases were diagnosed as cryptococcus neoformans meningitis, three cases were diagnosed as disseminated cryptococcal disease; five cases had underlying disease, including two cases of which were found to have primary immunodeficiency through high-throughput genetic analysis; headache with fever was the most common clinical symptom of cryptococcus neoformans meningitis.All three cases of disseminated cryptococcosis had primary or secondary immunodeficiency.All children were treated with 5-fluorocytosine + amphotericin B/amphotericin B liposome in induction therapy, and fluconazole in consolidation therapy.Notably, two cases showed hypokalemia during induction therapy, and one case showed mild renal dysfunction during consolidation treatment; five cases were cured, three cases were abandoned, one case was relapsed, and one case died.Conclusion:Children with cryptococcosis neoformans infection who are considered to have normal immune function may have primary immunodeficiency caused by gene mutation.PCR can improve the detection rate of cryptococcus neoformans and shorten the detection time.A significant increase in eosinophils may indicate the spread of infection.Hydration and regular potassium supplementation may reduce the toxicity of amphotericin B. Control of intracranial hypertension is the key to improve the success rate of treatment.

Objective:To explore the clinical characteristics of influenza A virus infection in children, and provide evidence for early identification of severe patients.Methods:A total of 114 patients with influenza like symptoms admitted to Shanghai Children′s Medical Center from October 2017 to May 2019 were enrolled in our study.All the patients were confirmed influenza A infection by Xpert-Xpress influenza/respiratory syncytial virus detection platform.The patients were divided into mild influenza A group (47 cases) and severe influenza A group (67 cases with pneumonia). The clinical data of these patients were analyzed and compared.Results:The male to female ratio of 114 cases was 1.28∶1.The age of included patients ranged from 1 month 12 days to 12 years old, and the median age was 3.00 (4.27) years old.The most common clinical manifestations were fever, cough and wheezing, accounting for 79.82%, 68.42%, and 43.00%, respectively.The rate of mixed infection was 24.56%, and adenovirus(5.26%), respiratory syncytial virus(4.39%) as well as mycoplasma(3.51%) accounted for the top three, and the rate of mixed bacterial infection was 12.28%.The median age of the patients in the severe influenza A group was 1.00 (3.58) years, and that in the mild influenza A group was 4.00 (5.00) years, with statistical difference ( Z=-3.81, P<0.001). The mixed infection rate was 38.80% (26/67) in severe influenza A group and 4.26% (2/47) in mild influenza A group, with statistical difference ( χ2= 17.8, P<0.001). The neutrophil/lymphocyte ratio in peripheral blood was 2.64 (3.37) in severe influenza A group and 1.17 (2.02) in mild influenza A group, with statistical difference ( χ2=-2.46, P=0.01). Conclusion:Children with smaller age, mixed infection and higher neutrophil/lymphocyte ratio are easy to develop into severe cases.The detection system of Xpert-Xpress influenza / respiratory syncytial virus is a simple, rapid and accurate method for influenza detection, which provides a good basis for diagnosis and treatment.

Objective:To explore the application value of FilmArray detection in children with acute lower respiratory tract infection and conduct economics analysis.Methods:From December 1, 2016 to November 30, 2017, 1 380 patients were enrolled in our study.Some children(FilmArray group) were tested for respiratory pathogens with FilmArray, while others (control group) were tested by 7-box antigen test of respiratory virus, gold colloid test of influenza and mycoplasma antibody.Those with underlying diseases were excluded.A total of 160 cases in the FilmArray group and 160 cases in the control group were obtained with tendency score matching method.The physical examination of pathogens, clinical indicators, usage of anti-infective drugs and hospitalization related costs were compared.Results:The positive rate of FilmArray test was significantly higher than that in control group (86.88% vs. 45.91%). The most common pathogens detected by FilmArray were adenovirus(39 cases), rhinovirus(34 cases), and parainfluenza virus(30 cases). In the FilmArray group, nine cases were positive for botulinum pertussis, accounting for 5.6% of the total.The hospitalization time of FilmArray group was shorter than that in control group [(8.89±6.23 days vs.(11.51±14.43)days]. In FilmArray group, the antibiotics were used for a shorter time, and 18 children did not use antibiotics during hospitalization.Compared with the control group, the hospitalization cost had no significant difference in the FilmArray group, but the antibiotic cost was less, as well as hospitalization time was shorter.The average hospitalization cost saved by using the FilmArray test was nearly 2 000 yuan per person. Conclusion:The application of FilmArray detection in children with acute lower respiratory tract infection can quickly and accurately identify a various infections of virus, bacteria and atypical pathogen, which guides using anti-infective drugs more reasonably.The application of FilmArray detection shortens the average hospitalization days of children, increases the utilization efficiency of medical resources, and reduces the medical cost and indirect economic loss of children’s families, which has certain economics significance.

Objective: To explore the clinical characteristics prevalence of respiratory adenovirus infections in children, and to provide reference for diagnosis, monitoring and timely treatment. Methods: A total of 775 clinical specimens were collected from Shanghai Children′s Medical Center during November 2016 to November 2017, and 84 cases of adenovirus infections were detected by FilmArray. Results: Among 775 samples, 84 samples(10.8%) were adenovirus positive.The epidemic peak of adenovirus infection was winter, spring and summer, especially in winter(20.8%), and the highest detection rate was in January(32.2%). The age ranged from 6 months to 2 years(47.7%). The common clinical manifestations are fever(91.7%), cough(96.4%), wheezing(48.8%), and shortness of breath(14.2%). A total of 15 cases(17.9%) had underlying diseases, the most of them were congenital heart diseases(9 cases, 10.7%). A total of 53 cases(63.1%) were mixed infection, and 21 cases(25.0%) were complicated with human rhinovirus/enterovirus.There were 5 cases of upper respiratory tract infection, 3 cases of bronchitis and 76 cases of pneumonia, including 55 cases of mild pneumonia and 21 cases of severe pneumonia.The average length of hospital stay was 8.3 days.The length of hospital stay, cases of wheezing and severe cases in the mixed infection group were significantly higher than those in the single infection group(P<0.05). Hospitalization days, wheezing symptoms, extrapulmonary symptoms, basic diseases and mixed infections in severe group were significantly higher than those in mild group(P<0.05). Conclusion Adenovirus infection occurs in children aged 6 months to 2 years, with a high incidence in winter, spring and summer.The peak month is January.The main clinical manifestations are high fever, cough and wheezing.Children with underlying diseases, wheezing symptoms, extrapulmonary symptoms and mixed infections are more likely to develop severe diseases.FilmArray detection system can quickly and accurately detect pathogens, provide a good basis for early diagnosis, monitoring and treatment of clinicians.

OBJECTIVE: To study the risk factors for gestational diabetes mellitus (GDM). METHODS: This retrospective case-control study was conducted among women registered at the Department of Obstetrics of West China Second University Hospital between March, 2016 and May, 2018. The women were divided into case group (GDM) and control group (Non- GDM) according to the diagnosis of GDM based on the International Association of Diabetes Pregnancy Study Groups (IADPSG) criteria. The data including age, education level, ethnicity and other socio-demographic data, as well as the gestational week, parity, polycystic ovary syndrome, family history of diabetes, fat mass, total body water, minerals and other clinical characteristics were collected. Univariate Logistic regression analysis was performed and the variables with statistical difference and clinical significance were included in multivariate Logistic regression analysis to identify the risk factors of GDM. RESULTS: A total of 3608 pregnant women were included in the study. Univariate logistic analysis revealed that age, previous GDM, intracellular/extracellular water, fat mass, arm circumference, skeletal muscle mass were risk factors for GDM. Multivariate logistic analysis showed that age, previous GDM, family history of DM, and an arm circumference ≥28.5 cm were independent risk factors for GDM after controlling pre-pregnancy BMI. The risk of GDM in pregnant women aged 30-35 years and 36 years or older was 1.883 ( < 0.001) and 2.883 ( < 0.001) times of that in women aged 20-29 years, respectively. Women with a history of GDM had a 6.604 ( < 0.001) greater risk of developing GDM than women without a history of GDM. Compared with those without a family history of diabetes, those with a family history of diabetes were 2.518 times more likely to develop GDM ( < 0.001). Compared with those with an arm circumference no greater than 25.5 cm, pregnant women with an arm circumference over 28.5 cm had an increased risk of GDM by 2.815 times ( < 0.001). High fat free mass was a protective factor for GDM, and compared with a fat free mass below 35.1 kg, a fat free mass over 40.1 kg was associated with a lowered risk of GDM by 0.515 times ( < 0.001). CONCLUSIONS The onset of GDM was affected by multiple factors. Age, history of GDM, family history of diabetes, and a large arm circumference are all independent risk factors of GDM and should be controlled to reduce the incidence of GDM.
Adult ; Body Mass Index ; Case-Control Studies ; China ; Diabetes, Gestational ; Female ; Humans ; Pregnancy ; Retrospective Studies ; Risk Factors ; Young Adult

ObjectiveTo explore the mastery of disease-related knowledge among tuberculosis children's parents so as to provide a basis for carrying out health education for children's parents. MethodsFrom November to June 2016, we selected tuberculosis children's parents at West China Second University Hospital of Sichuan University as subjects by convenience sampling. A total of 112 children's parents were investigated with the self-designed questionnaire by interview. A total of 112 questionnaire were sent out and all of them were collected with 100% for the recovery rate. ResultsAmong 112 children's parents, they mastered well on key points in the treatment, whether could be cured, medicine taking time and requirements for review of tuberculosis with above 90% for the accuracy rate. There were statistical differences in the mastery of disease-related knowledge among children's parents with different education levels and nations (P＜ 0.05). ConclusionsWe should focus on strengthening health education of tuberculosis knowledge for parents with low educational levels, in rural areas and minority nationalities, and health education of drug-related knowledge for all parents. We should develop targeted health education to improve the compliance of tuberculosis treatment in children.

Objective To investigate the efficacy and safety of leukoreduction therapy in severe per-tussis in infants. Methods Therapeutic processes of 3 cases of severe pertussis in PICU of Shanghai Children′s Medical Center were retrospectively studied from October 2017 to May 2018. We reviewed the related literatures and summarized the time and effectiveness of leukoreduction therapy in severe pertussis. Results All 3 cases had leukocytosis,respiratory faliure,pulmonary hypertension and right heart failure. One case had multiple organ failure before undergoing exchange transfusion therapy and eventually died. Two cases that had pulmonary hypertension during the period of WBC′s rising accepted leukopheresis therapy before multiple organ failure,and eventually survived. We reviewed the foreign literatures which was almost case reports,leukoreduction therapy might improve the prognosis of severe pertussis in infants,but the time of using it had no conclusion. Conclusion This is the first report of leukoreduction therapy for the severe per-tussis in infants in China. It provides a new method for the treatment of severe pertussis in infants. It is worth looking forward to use this method combined with continuous renal replacement therapy and extracorporeal membrane oxygenation technology. In the future,multicenter clinical research should be done to explore the effectiveness and safety of leukoreduction therapy in the severe pertussis in infants.

Through interviews with 30 pregnant nurses, this paper found that pregnant nurses needed humanistic care in four aspects of worrying about their own health and their fetus' health, psychological pressure, career planning and interpersonal relationships. And this paper put forward humanistic care countermeasures, including that nursing administrators should remind pregnancy nurses to pay attention to the health of themselves and their fetal, and pay attention to the psychological health of the pregnancy nurses, increasing the humanistic care to the pregnancy nurses can reduce the turnover rate of nurses, and family and society should provide humanistic care support for pregnant nurses.

Breastfeeding provides many health benefits for infants and mothers, and is the most ideal way of feeding infants. At present, domestic and foreign studies have shown that maternal and family members of the breastfeeding awareness and behavior has improved, but the overall feeding rate is still low. There are many factors influencing maternal breastfeeding, in which gestational breastfeeding willingness is one of the important predictors of postnatal breastfeeding. The first step to achieve successful breastfeeding is to establish a prenatal breastfeeding willingness, so determining the factors influencing breastfeeding willingness during pregnancy can provide a theoretical basis for further improvement of breastfeeding rate. This article reviewed the willingness and influencing factors of breastfeeding among pregnant women in China and abroad, in order to provide the basis for formulating prenatal care strategies and health education, so as to improve the breastfeeding rate.