Objective:To analyze the clinical characteristics and influencing factors on postoperative emotional and cognitive function for elderly versus non-elderly male patients with laryngeal carcinoma.Methods:The patients with laryngeal cancer hospitalized in the Department of Otorhinolaryngology-Head and Neck Surgery were selected for a questionnaire survey in two Grade III-A Hospital in Shanxi Province from January 2018 to December 2019.There were 105 patients with laryngeal cancer, including 60 in the elderly and 45 in the non-elderly group.Negative emotion and cognitive function were investigated by using Self-rating Anxiety Scales(SAS), Self-rating Depression Scale(SDS), and Montreal Cognitive Assessment(MOCA)before surgery, 10 days after surgery, and 1 year after surgery, respectively.The clinical characteristics of the elderly versus non-elderly groups were analyzed and compared.Results:One year after surgery, there were 52 cases(86.7%)and 27 cases(45.0%)of depression and cancer-related cognitive impairment(CRCI)in the elderly group, which were higher than 30 cases(66.7%)and 4 cases(8.9%)in the non-elderly group, with statistically significant difference( χ2 = 6.013, χ2 =16.115 and P<0.05, P<0.01). The elderly group showed much more anxiety and CRCI 10 days after surgery(60.50±4.31 vs.55.84±3.81, 27.47±1.08 vs.28.31±1.08, P<0.01)and showed much more depression and CRCI(57.20±5.66 vs.62.60±5.37, 27.36±1.37 vs.26.08±1.42, P<0.01)than did the non-elderly group 1 year after surgery.The results of multiple linear regression analysis in the elderly group showed that the mode of operation and the pronunciation one year after surgery significantly affected their anxiety; the marriage and the educated level significantly affected their depression; the marriage, the educated level and operation mode significantly affected their cognitive function.Clinical stage of tumor significantly affected anxiety and depression, the educated level significantly affected cognitive function in the non-elderly group. Conclusions:Postoperative negative emotion and cognitive function in patients with laryngeal cancer continues to deteriorate after surgery, which were affected by many factors, especially in elderly patients.It is necessary to conduct active and effective interventions as soon as possible.

Early intervention contributes to improving patient experience and doctor-patient relationship in the case of non-psychiatric outpatients with psychological problems.The authors studied the psychological assessment and hierarchical management for non-psychiatric inpatients at a general hospital. Measures taken include establishing multi-disciplinary and inter-department teams, building an intra-hospital joint-action system, and implementing the psychological assessment and hierarchical management for non-psychiatric inpatients.These efforts explored ways for a general hospital in psychological counseling, offering humanistic service, and transformation of medical pattern.

Objective? To explore the correlation between preoperative cognitive function and anxiety and depression in patients with laryngocarcinoma. Methods? Totally 42 patients with laryngocarcinoma who were hospitalized in the Department of Otorhinolaryngology Head and Neck Surgery of 2 ClassⅢ Grade A Hospitals in Shanxi Province from September 2017 to September 2018 were selected into the observation group by convenient sampling, while 40 healthy volunteers were included in the control group. They were investigated with Self-Rating Anxiety Scale (SAS),Self-Rating Depression Scale (SDS) and Montreal Cognitive Assessment (MOCA). Results? The preoperative SAS and SDS scores of the observation group were (41.48±5.46) and (43.69±6.16) respectively, both higher than those of the control group (t=4.189, 6.234; P＜ 0.01); the preoperative MOCA score of the observation group was (22.90±4.13), lower than that of the control group (t=2.646, P＜ 0.01). Correlation analysis showed that the laryngocarcinoma patients＇ anxiety and depression were negatively correlated with their cognitive function (r=-0.750, -0.660; P＜0.01). Conclusions? Compared with the healthy volunteers, the laryngocarcinoma patients are more susceptible to anxiety, depression and cognitive disorder. The severer their anxiety and depression, the poorer their overall cognitive function was.

Objective To evaluate the clinical outcomes of endoscopic and microsurgical treatments in patients with hypertensive basal ganglia hemorrhage (HBGH).Methods A retrospective analysis of clinical features of 37 patients received microsurgical treatment via transsylvian-transinsular approach or 32 patients received endoscopic treatment for evacuation of HBGH in our hospital from January 2011 to January 2015 was performed.The operation time,hematoma clearance rate,re-bleeding rate and prognoses of the patients were investigated.Results As compared with patients accepted microsurgical treatment,patients accepted endoscopic treatment had significantly shorter operation time,smaller peroperative bleeding volume,and shorter hemostatic time (P＜0.05).The preoperative Glasgow coma scale (GCS) scores in patients accepted endoscopic treatment and microsurgical treatment were 8.63±1.24 and 8.67±1.31,without significant difference (P＞0.05);24 h after operation,GCS scores in patients accepted endoscopic treatment increased to 12.79±1.20,which had significant difference as compared with those in patients accepted microsurgical treatment (11.23±1.29,P＜0.05).The cerebral edema volume in patients accepted endoscopic treatment and microsurgical treatment was (11.83±4.08) mL and (18.76±7.92) mL,with significant difference (t=6.460,P=0.000).The hematoma clearance and prognosis in patients accepted endoscopic treatment were better than those in patients accepted microsurgical treatment.Conclusion Endoscopic evacuation ofhematoma for HBGH is efficient and safe,enjoying better efficacy than microsurgery.

Objective:To investigate the recognition and injury prevention strategies of hepatic artery variations during hepatic portal lymphadenectomy. Methods:A retrospective analysis was performed, and 12 patients of hepatic arterial variation among 62 pa-tients with hepatic portal lymphadenectomy were the subjects. The study was conducted in the First Affiliated Hospital of Bengbu Medi-cal College between January 2013 and July 2014. The intraoperative treatment and postoperative complications were recorded. Results:Among 12 cases of hepatic artery variation, we found the following cases:3 cases (25.0%) of Michels' Type III, 2 cases (16.7%) of Mi-chels' Type VI, 1 case (8.3%) of Michels' Type IX, 1 case (8.3%) of Hiatt's Type 6, 2 cases (16.7%) of spatial location variation between right hepatic artery and hepatic duct, 2 cases (16.7%) of left and right hepatic artery originating from a common hepatic artery, and 1 case (8.3%) of right hepatic artery originating from the gastroduodenal artery. No injury of hepatic artery occurred. Two cases had post-operative complications, including 1 case of pancreatic leakage and 1 case of incision infection;postoperative hemorrhage, bile leakage, hepatic abscess did not occur in these two cases. Patients recovered well in general. Conclusion:Hepatic arterial injury can be signifi-cantly reduced by the following:increased familiarity with the various types of hepatic artery variations;complete imaging examina-tions for inspection and evaluation before surgery;and careful and meticulous operations in surgery.

OBJECTIVE: To select relatively specific biomarkers in serum from laryngeal carcinoma patients using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology. METHOD: The serum samples of 32 cases of laryngeal carcinoma preoperation and 15 cases of laryngeal carcinoma 10 days postoperation and 40 healthy controls on IMAC-Cu protein chip were collected. The spectrometric protein profiling was detected by SELDI-TOF-MS, the data was analyzed by Biomarker Wizard software and Biomarker Pattern software. RESULT: Twenty-six differentially expressed proteins in serum were screened by analysis of protein spectra of preoperative and postoperative patients and healthy controls. Eight proteins (4048.09, 5073.79, 5339.29, 3795.38, 9187.24, 15323.8, 6111.92, 6943.87) were obtained for making up patterns that was able to class the preoperative-team and postoperative-team. Corresponding correct ratio were 75% (30/40), 71.88% (23/32) and 66.67% (10/15). CONCLUSION SELDI-TOF-MS technology is a quick, easy, convenient and high output analyzing method that it is capable of selecting several relatively specific, potential biomarkers from the serum of laryngeal carcinoma patients and may have clinic value.
Aged ; Biomarkers, Tumor ; blood ; Case-Control Studies ; Female ; Humans ; Laryngeal Neoplasms ; blood ; Male ; Middle Aged ; Protein Array Analysis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

Objective:To screen the tumor markers of laryngeal carcinoma and to investigate their expression in preoperative and postoperative serum.Method:The distinct protein in serum was detected in 32 cases of laryngeal carcinoma and 38 healthy people by IMAC-Cu proteinchip array and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS).The distinct proteins in serum were detected in 32 cases of laryngeal carcinoma preoperation and within 10 days 15 cases of laryngeal carcinoma postoperation with the same methods.The discriminatory profiling between preoperative and postoperative patients was analyzed by Biomarker Wizard software and Biomarker Pattern software.Result:The results showed that fifteen differentially expressed proteins in serum were screened by analysis of protemic spectra of preoprative patients and normal subjects.Seventeen kinds of protein differentially expressed in serum were screened by analysis of protemic spectra of preoperative and postoperative patients.Six kinds of protein(2 958.52,3 796.89,5 148.86,6 115.57, 052.18,and7 770.76)were obtained for making up patterns that was able to class the preoperative-team and postoperativeteam.Corresponding correct ratio were 84.38%(27/32)and 73.33%(11/15).Conclusion:The preliminary results suggest that classification system will provide a highly accurate and innovative approach for the arly dioagnosis of laryngeal carcinoma and judgement of prognosis.SELDI-TOF-MS technology is a useful tool for a high throughput screening of large-sized serum samples tO discover potential biomarkers for laryngeal carcinoma.

OBJECTIVE: To screen the tumor markers of laryngeal carcinoma and to investigate their expression in preoperative and postoperative serum. METHOD: The distinct protein in serum was detected in 32 cases of laryngeal carcinoma and 38 healthy people by IMAC-Cu proteinchip array and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The distinct proteins in serum were detected in 32 cases of laryngeal carcinoma preoperation and within 10 days 15 cases of laryngeal carcinoma postoperation with the same methods. The discriminatory profiling between preoperative and postoperative patients was analyzed by Biomarker Wizard software and Biomarker Pattern software. RESULT: The results showed that fifteen differentially expressed proteins in serum were screened by analysis of proteomic spectra of preoperative patients and normal subjects. Seventeen kinds of protein differentially expressed in serum were screened by analysis of proteomic spectra of preoperative and postoperative patients. Six kinds of protein (2958.52, 3796.89, 5148.86, 6115.57, 4052.18, and 7770.76) were obtained for making up patterns that was able to class the preoperative-team and postoperative-team. Corresponding correct ratio were 84.38% (27/32) and 73.33% (11/15). CONCLUSION The preliminary results suggest that classification system will provide a highly accurate and innovative approach for the early diagnosis of laryngeal carcinoma and judgement of prognosis. SELDI-TOF-MS technology is a useful tool for a high throughput screening of large-sized serum samples to discover potential biomarkers for laryngeal carcinoma.
Aged ; Biomarkers, Tumor ; blood ; Female ; Humans ; Laryngeal Neoplasms ; blood ; pathology ; Male ; Middle Aged ; Prognosis ; Protein Array Analysis ; methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

OBJECTIVE: In order to establish a primary diagnosis model of laryngeal carcinoma, we explore the changes of proteomic spectra in serum from patients with laryngeal carcinoma and healthy controls. METHOD: Proteomic spectra of 32 serum samples from laryngeal carcinoma (18 cases of glottis and 14 of supraglottis) patients and 38 from healthy people whose age and sex was matched were generated by IMAC3-Cu protein chip array and SELDI-TOF-MS (surface was enhanced laser desorption/ionization time-of-flight mass spectrometry). The discriminatory profiling between cancer and normal samples was analyzed by Biomarker Wizard software and Biomarker Pattern software. RESULT: Fifteen differentially expressed proteins in serum were screened by analysis of proteomic spectra of preoperative patients and normal subjects. Eight proteins (3795.04, 5068.18, 5339.78, 5908.28, 6629.74, 9266.32, 13879.6 and 14037.1) were obtained for making up patterns that was able to class the patients team and normal team correctly. Corresponding correct ratio were 87.50% (28/32) and 86. 84% (33/38). CONCLUSION SEI.DI-TOF-MS Protein Chip technology is a new tool for the early diagnosis of laryngeal carcinoma.
Aged ; Biomarkers, Tumor ; analysis ; blood ; Carcinoma, Squamous Cell ; blood ; metabolism ; pathology ; Case-Control Studies ; Female ; Humans ; Laryngeal Neoplasms ; blood ; metabolism ; pathology ; Male ; Middle Aged ; Protein Array Analysis ; Proteomics ; methods ; Software ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Problem-Based Learning(PBL)is a new teaching mode.Application of PBL in Laboratory Diagnostics is a practical teaching reform.Students'learning mainly by themselves is PBL’s main feature.The status and role of teachers have a qualitative change.They are not teaching but guiding.Therefore teachers must realize the change of their roles in PBL.