Objective:To study the short-term clinical outcomes of different courses of antenatal corticosteroids (ACS) for preterm twins.Methods:From January 2017 to December 2021, preterm twins with gestational age (GA) 24-34 weeks admitted to the neonatal ward of our hospital and received ACS were retrospectively studied. The infants were assigned into single-course group, partial-course group and multiple-course group according to ACS courses. The short-term clinical outcomes were compared among the groups. SPSS software version 25.0 was used for statistical analysis.Results:A total of 286 infants were enrolled in this study, including 128 in single-course group, 89 in partial-course group and 69 in multiple-course group. Compared with single-course group, the risks of neonatal respiratory distress syndrome (RDS) in both partial-course group ( OR=2.332, 95% CI 1.028-5.293, P=0.043) and multiple-course group ( OR=3.872, 95% CI 1.104-13.584, P=0.034) were higher. The birth length in multiple-course group ( β=-0.016, 95% CI -0.029 - -0.002, P=0.024) was lower than single-course group. Conclusions:The risks of neonatal RDS in preterm twins are higher in partial-course and multiple-course of ACS. A full course of ACS should be used to prevent neonatal RDS until further evidence of effectiveness is available.

Objective To compare the effects of sufentanil and fentanyl anesthesia on blood glucose and insulin levels in patients undergoing laparoscopic cholecystectomy,and explore the mechanism using metabonomics technique.Methods Total 60 patients,37 males and 23 females,ASA physical status Ⅰ or Ⅱ undergoing laparoscopic cholecystectomy were randomly divided into sufentanil and fentanyl groups,and 30 cases in each group.Venous blood was collected before anesthesia and 5 min,40 min and 2 h after anesthesia,which was used to detect blood glucose,insulin levels and metabonomics analysis.Results Blood glucose were significantly lower in sufentanil group than in fentanyl group at 5 min after intubation,40 min in surgery,and postoperative 2 h (P＜0.05).After 2 h,insulin in sufentanil group was significantly lower than that in fentanyl group (P＜0.05).Metabolic analysis showed that in the positive ion mode,using orthogonal partial least squares discriminant analysis,the patients in the two groups were classified according to the serum metabolites at postoperative 2 h,which showed that the serum metabolites in patients of different groups were significantly different,and 5 biomarkers closely related to blood glucose and insulin were found.Conclusion Sufentanil showed slighter effects on blood glucose and insulin in patients,and more suitable for cholecystectomy patients.The mechanism may be related with the glucose and insulin metabolism.

Objective Acquiring genetic information of Y-SNPs and Y-STRs genetic makers from samples with the surname of Kong, which is useful for exploring the correlation between surname and Y chromosome in forensic applications studies.Methods Two multiplex genotyping assays and SNaPshot assay were used to analyze 255 unrelated male blood samples who share the same surname Kong and 330 unrelated male blood samples obtained randomly. 17 Y-STRs were typed for the surname Kong population samples. The software Arlequin 3.5.1.2 and the program Network 4.6.1.1 were used for data statistical analysis.Results 13 haplogroups were observed. The highest haplogroup frequency in the two populations were O3a2c1a-M117 (21.57%, 14.85%). 196 haplotypes in Kong population deifned by 17 Y-STRs locus were obtained and the haplotype diversity was 0.9939. 14-12-25-28-19-15-12-19-12-11-12-22-12-11-14-10-19 is the typical haplotype. Median Joining algorithm and Mismatch Distribution were adopted to analyze the Y-STR haplotye under haplogroup O3-M122, and the result shows that there are two “central star” distribution. Conclusion Combined with Y-SNP and Y-STR analysis showed that the Kong population had experienced complicated exchanges and expansion or continued growth, which has more than one surname origin. Hence, its population genetic structure and historical differences have potential applications in forensic science.