Objective: To investigate the effects of retinoid-related orphan receptor γ (RORγ) gene on proliferation and metastasis of human colon cancer cells． Methods: RORγ knockdown cell lines were constructed and the knockdown efficiency was detected by RT-qPCR and Western blot assays ; MTT，colony formation，Transwell and wound healing assays were used to detect cell proliferation and metastasis ; the expression of epithelial-mesenchymal transition (EMT) related proteins was detected by Western blot．The relationship between RORγ gene expression and immune cell infiltration in tumor microenvironment was analyzed using TIMER 2. 0 database. Results : The knockdown of RORγ enhanced the viability (F = 157. 40，P＜0. 01) ，clonogenesis (F = 61. 35，P＜0. 01) ，migration (F = 13. 00，P＜0. 01) ，invasion (F = 21. 26，P＜0. 01) and wound healing ability (F = 877. 2，P＜0. 01) of colon cancer cells，inhibited the expression of E-Cadherin，and promoted the expression of vimentin and N-Cadherin．TIMER 2. 0 database analysis showed that RORγ expression in colon adenocarcinoma ( COAD) tissues was associated with multiple immune cell infiltrates． Conclusion Downregulation of RORγ expression promoted the proliferation and metastasis of colon cancer cells.

Objective To investigate the etiology and epidemic characteristics of infectious diarrhea in children in Suzhou, and to provide a basis for accurate prevention and control. Methods Active surveillance of infectious diarrhea pathogens was carried out in a national sentinel surveillance hospital in Suzhou from 2018 to 2020. Epidemiological investigation was completed and stool samples were collected. All samples were detected and classified for 5 viruses and 6 types of bacteria. Results A total of 999 cases of infectious diarrhea were included, the detection rate of pathogen was 36.34%, virus was 31.41%, bacteria was 6.31%, and multi-pathogen mixed infection was 4.8%. The top three pathogens were rotavirus (17.92%), norovirus (12.51%) and Salmonella (3.4%). Both rotavirus diarrhea and norovirus diarrhea occurred most frequently in children aged 7-24 months, with peak incidence in winter and early spring. There was no obvious seasonal and age distribution of bacterial diarrhea. The multi-pathogen mixed infection was mainly virus-virus. G9[P]8 was the dominant genotype of rotavirus, while type 2 was the dominant type of norovirus in the cases of infectious diarrhea. Conclusion Infectious diarrhea in children under 5 years old in Suzhou area from 2018 to 2020 is mainly caused by viruses, among which rotavirus and norovirus are the dominant pathogens. Viral diarrhea has obvious population distribution and seasonal characteristics. The dominant pathogens of bacterial diarrhea are Salmonella and diarrheagenic Escherichia coli.

AIM: To investigate the incidence of amblyopia in preschool children and correlation factors in Bao';an District of Shenzhen so that to provide possible basis for the prevention of regional amblyopia.

METHODS: Totally 818 preschoolers with visual acuity screening were selected and studied from June 2017 to February 2018 in our Hospital. The incidence of amblyopia in children was recorded with the amblyopia diagnostic criteria and they were treated as observation groups. The others were treated as controls. The single factor and multivariate Logistic regression analysis were performed for the two groups.

RESULTS:The incidence of amblyopia in preschool children in Bao';an District of Shenzhen was 5.7%(47/818). Among them, ametropic amblyopia was the most common(63.8%), the second was anisometropic amblyopia(21.3%), the third was strabismic amblyopia(12.8%)and in the end was form deprivation amblyopia(2.1%). By single factor analysis: there were statistically significant differences between two groups for the correlation factors of gestational age, gravidity and parity history, gestational weeks and nearsightedness(amblyopia)family history(P<0.05). By multivariate Logistic regression analysis: the older gestational age, more gravidity and parity, shorter gestational weeks and positive nearsightedness(amblyopia)family history were the highest risk factor for children with amblyopia(P<0.05).

CONCLUSION: The incidence of amblyopia in preschool children in Bao';an District of Shenzhen is high and it should be taken seriously. In the meantime, there are many influencing factors. But comprehensive preschool vision screening is an effective method for early discovery, early diagnosis and early treatment.