Objective To explore the application effect of detailed nursing on ensuring nursing safety in operation room.Methods 200 patients were divided into control group (50 pa-tients)and observation group(150 patients)according to different nursing methods in operation room.The control group was received routine nursing while the observation group was given de-tailed nursing based on the control group,and nursing quality and satisfaction were compared be-tween the two groups.Results Instrument preparation,nurse coordination skills,equipment management and quality score of disinfection and isolation and safety score of nursing quality in the observation group were higher than that in the control group,the difference was statistical signifi-cant (P <0.05).The difference was not statistical significant in operation time between two groups (P >0.05).Score of image and occupation accomplishment of nursing staff,intraoperative nursing comprehensive score and nursing satisfaction in the observation group were higher than that in the control group,the result revealed that the difference was statistically significant(P <0.05). Conclusion Detailed nursing in operation room can ensure the operation safety and increase pa-tients’satisfaction.

Objective To explore the application effect of detailed nursing on ensuring nursing safety in operation room.Methods 200 patients were divided into control group (50 pa-tients)and observation group(150 patients)according to different nursing methods in operation room.The control group was received routine nursing while the observation group was given de-tailed nursing based on the control group,and nursing quality and satisfaction were compared be-tween the two groups.Results Instrument preparation,nurse coordination skills,equipment management and quality score of disinfection and isolation and safety score of nursing quality in the observation group were higher than that in the control group,the difference was statistical signifi-cant (P <0.05).The difference was not statistical significant in operation time between two groups (P >0.05).Score of image and occupation accomplishment of nursing staff,intraoperative nursing comprehensive score and nursing satisfaction in the observation group were higher than that in the control group,the result revealed that the difference was statistically significant(P <0.05). Conclusion Detailed nursing in operation room can ensure the operation safety and increase pa-tients’satisfaction.

OBJECTIVETo investigate common chromosomal changes and the LOH frequency of microsatellite loci in primary gastric cancer samples in order to locate the deleted regions in which human gastric cancer related genes might exist.

METHODSComparative genomic hybridization (CGH) was used to define global chromosomal aberrations in 43 primary gastric tumors. Based on the results of CGH, analysis of loss of heterozygosity (LOH) was performed in chromosome 19 in which the loss was first discovered in the gastric cancers. The PCR-based approach was used to investigate 22 loci, which are spaced at 1.1 - 10.9 cM intervals throughout chromosome 19. The amplified PCR fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan trade mark and Genotyper trade mark.

RESULTSCGH analysis revealed gains in chromosome 3p (8/43), 8q (8/43), 20 [20 (9/43), 20p (7/43), 20q (4/43)], 12q (16/43), 13q (12/43) and losses in 19 [19 (15/43)], 7 [17 (8/43), 17p (10/43)], 16 (10/43) and 1p (11/43). Among the 43 evaluated samples, the most frequent LOH was detected at locus D19S571 (27.81%).

CONCLUSIONSThe tumorigenesis of gastric cancer includes several chromosomal changes. The aberration of chromosome 19 was the first common change founded in gastric cancer. The region near the D19S571 might harbor potential genes related to the tumorigenesis of gastric cancer.

Chromosomes, Human, Pair 19 ; Humans ; Loss of Heterozygosity ; Nucleic Acid Hybridization ; Peutz-Jeghers Syndrome ; genetics ; Stomach Neoplasms ; genetics