Oleanolic acid was chemically modified to improve its interaction with bovine serum albumin,thus to increase the mutual binding ability.As a lead compound,oleanolic acid reacted with Jones-reagent,then methyl iodide and finally hydroxylamine to obtain four different derivatives.Their structures were confirmed by IR and 1H NMR analysis.The interaction between these derivatives and bovine serum albumin and the effect of temperature and trace metal ions as Cu2+ and Co2+ on the interaction were investigated by fluorescence spectroscopy.All these derivatives exhibited improvement of the interaction compared with oleanolic acid with 16 times stronger for OA2.The interaction also increased with the presence of trace metal ions as Cu2+ and Co2+.These results indicate that chemical modification can improve the interaction of oleanolic acid with bovine serum albumin.

Objective To explore the cause of bile acid-induced lung injury through investigating the cell apoptosis and the expressions of Capsase-3 in A549 cell of umbilical artery serum in neonates delivered by women with intrahepatic cholestasis of pregnancy.Methods A549 cell was used as target cell.The cultural cells in orifice were divided into control group and intrahepatic cholestasis of pregnancy-serum attacking group.The cells of control group were cultivated with normal nutritive medium.The umbilical arterial blood was cowered from the placental end of pregnant women with intrahepatic cholestasis after the baby had been delivered.Then the serum was gathered,and the cells of the intrahepatic cholestasis of pregnancy-serum attacking group were attacked by intrahepatic cholestasis of pregnancyserum.After 24 hours,lactate dehydrogenase leakage rate,expression of Caspase-3 and the apoptosis rate of the cells in the 2 groups were detected,respectively.Results The expression of Caspase-3 in A549 cells was observed in the light microscope,and Caspase-3 expre-ssion in the cytoplasm was brown.The lactate dehydrogenase leakage rate [(34.68 ±0.77) ％],the integrate optical density value (981.77 ± 55.21) of the expression of Caspase-3 and the rate of apoptosis [(27.86 ± 0.53) ％] of cells in intrahepatic cholestasis of pregnancy group were significantly higher than those of the cells in control group[(17.39±0.66)％,(540.63 ±38.41),(6.99 ±0.11)％] (t =-45.70,-15.96,-134.41,all P ＜ 0.05).Conclusions Umbilical artery serum in neonates delivered by women with intrahepatic cholestasis of pregnancy can induce apoptosis of A549 cells by up-regulating the expression of Caspase-3,and this was the potential machine of bile acid-induced lung injury in newborn infants.

In order to screen the genes differentially expressed in two human prostate cancer cells with different metastasis potentials, suppression subtractive hybridization (SSH) was done twice on human prostate cancer cell line with high potential of metastasis PC3M-1E8 and its synogenetic cell line PC3M-2B4 with low metastasis potential. In the first subtraction PC3M-2B4 was used as tester and PC3M-1E8 as driver and the forward subtractive library was constructed. In the second on the tester and driver were interchanged and the reverse subtractive library was constructed. The screened clones of both libraries were sequenced and Gene Bank homology search was performed. Some clones were confirmed by quantitative real-time PCR. The results showed that two subtractive libraries containing 238 positive clones were constructed. Analysis of 16 sequenced clones randomly picked from two libraries showed that 4 differentially expressed gene fragments were identified as new EST with unknown functions. It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
Cell Line, Tumor ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Gene Library ; Neoplasm Metastasis/*genetics ; Nucleic Acid Hybridization/*methods ; Prostatic Neoplasms/*genetics ; Prostatic Neoplasms/pathology

In order to screen the genes differentially expressed in two human prostate cancer cells with different metastasis potentials, suppression subtractive hybridization (SSH) was done twice on human prostate cancer cell line with high potential of metastasis PC3M-1E8 and its synogenetic cell line PC3M-2B4 with low metastasis potential. In the first subtraction PC3M-2B4 was used as tester and PC3M-1E8 as driver and the forward subtractive library was constructed. In the second one the tester and driver were interchanged and the reverse subtractive library was constructed. The screened clones of both libraries were sequenced and Gene Bank homology search was performed. Some clones were confirmed by quantitative real-time PCR. The results showed that two subtrac-tive libraries containing 238 positive clones were constructed. Analysis of 16 sequenced clones ran-domly picked from two libraries showed that 4 differentially expressed gene fragments were identi-fied as new EST with unknown functions. It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.