PURPOSE: The purpose of this study was to evaluate the reproducibility and validity of a short food frequency questionnaire (FFQ) for food group intake in Japan, the reproducibility and partial validity of which were previously confirmed for nutrients. METHODS: A total of 288 middle-aged healthy volunteers from 11 different areas of Japan provided nonconsecutive 3-day weighed dietary records (DRs) at 3-month intervals over four seasons. We evaluated reproducibility based on the first (FFQ1) and second (FFQ2) questionnaires and their validity against the DRs by comparing the intake of 20 food groups. Spearman's rank correlation coefficients (SRs) were calculated between energy-adjusted intake from the FFQs and that from the DRs. RESULTS: The intake of 20 food groups estimated from the two FFQs was mostly equivalent. The median energy-adjusted SRs between the FFQ1 and FFQ2 were 0.61 (range 0.38-0.86) for men and 0.66 (0.45-0.84) for women. For validity, the median de-attenuated SRs between DRs and the FFQ1 were 0.51 (0.17-0.76) for men and 0.47 (0.23-0.77) for women. Compared with the DRs, the proportion of cross-classification into exact plus adjacent quintiles with the FFQ1 ranged from 58 to 86% in men and from 57 to 86% in women. According to the robust Z scores and the Bland-Altman plot graphs, the underestimation errors in the FFQ1 tended to be greater in individuals with high mean levels of consumption for meat for men and for other vegetables for both men and women. CONCLUSION The FFQ demonstrated high reproducibility and reasonable validity for food group intake. This questionnaire is short and remains appropriate for identifying associations between diet and health/disease among adults in Japan.
Adult ; Aged ; Diet/statistics & numerical data* ; Diet Surveys ; Energy Intake ; Female ; Food/statistics & numerical data* ; Healthy Volunteers ; Humans ; Japan ; Male ; Middle Aged ; Reproducibility of Results

The scientific basis of moxibustion using moxa was investigated, focusing on the characteristics and thermal stimulation properties of moxa, its biochemical characteristics and actions, and morphological changes in skin tissue treated with moxibustion. The presentations by and viewpoints of the symposists are outlined below:
1) Characteristics and thermal stimulation properties of moxa:The thermal stimulation properties of Artemisia princes Pamp., moxa, materials other than Artemisia princes Pamp., and materials similar to moxa were presented. Re-investigation of the results of common views of Artemisia princes Pamp. and moxa by experiments and a survey were also presented.
2) Biochemical characteristics and actions of moxa:Chemical substances contained in Artemisia princes Pamp. and moxa, and diverse physiological actions of moxa, such as an anti-oxidative stress action, signal transduction, activation of energy metabolism, and anti-inflammatory action, were presented with viewpoints. In addition, biochemical actions were reviewed based on the extraction of new chemical components.
3) Morphological changes in skin tissue treated with moxibustion:Moxibustion-induced morphological changes in the epidermis, connective tissue, blood vessels, and nerve tissue were presented. Regarding skin tissue regeneration, viewpoints were presented, emphasizing that actions of corneocytes and fibroblasts are particularly important, in addition to revascularization, nerve fibers are not regenerated unless the skin tissue is repaired to a certain degree.

A symposium was held to focus attention on the position of the meridian point GV 14, which has been con-troversial over the centuries.
To summarize the symposium opinions : (1) Since the positions of meridian points have changed with time and culture and according to the therapeutic target, objective, and instruments, the changes of the position of GV 14 from above the spinous process of the second cervical vertebra to between the spinous processes of the 6 th and 7 th cervical vertebrae and to between the spinous processes of the 7 th cervical and the 1 st thoracic vertebrae are not exceptional. (2) If the position of GV 14, which is a standard for the determination of meridian points on the back, changes, its clinical value is nullified. It should be, therefore, standardized as being between the spinous processes of the 6 th and 7 th cervical vertebrae. (3) The 6 th cervical vertebra is important in body surface clinical science, and the 7 th cervical vertebra is important in the mobility and anatomical segmentation of the spine. However, in consideration of the development processes of acupuncture, it is more reasonable to determine the position of GV 14 on the basis of clinical effects. (4) The proposed international standardization of the position of GV 14 as between the spinous processes of the 7 th cervical and 1 st thoracic vertebrae is acceptable, because there has been no objection from China or Korea.
No agreement was reached in this symposium, but the position of GV 14, which bears great clinical significance, must be evaluated further.

A seminar was held with “the development of research on auricular acupuncture” as the main theme. In this seminar, the recent development of auricular acupuncture in Japan and abroad, its action mechanism, and clinical effects were reviewed, and the findings were comprehensively evaluated.
Concerning basic research on the effects of auricular acupuncture on obesity, the relationship between the auricle and hypothalamo-autonomic system and individual variation associated with differences in the condition of patients receiving the treatment were reported. As for the action mechanism, leptin expressed in white adi-pose tissue (WAT) due to acupuncture stimulation of the auricle was reported to bind to leptin receptors (Ob-R) in both peripheries and the center and suppress food intake.
Concerning clinical effects of auricular acupuncture, the results reported in Japan indicating its effectiveness for the treatment of obesity were presented. However, no overall conclusion was reached, because papers published abroad were not consistent in the therapeutic procedure or evaluation parameters. Auricular acupuncture appeared to be clinically effective for analgesia and the treatment of drug addiction but ineffective as an anti-smoking treatment.

This symposium was held as the second in a three part series at Kagawa Conference (June 6-8, 2003). During the Tsukuba conference (2002), distal area treatment was the topic of focus. The criteria and evidence for the effectiveness of distal area treatments were presented. Three symposists and one minor speaker reported on the effectiveness of distal area treatments from clinical and basic viewpoints. However, another symposist showed the superiority of local area treatments and denied the effectiveness of distal area treatments. There fore, we decided that a succinct and definitive discussion on distal area treatments (etc) should be presented during the second symposium. To this end, long time members of JSAM were given a questionnaire survey prior to the conference. Respondents were asked to define their own use of local and distal treatment, to hypothesize on the curing mechanism involved and to state which practice methods they use most.
Mr. Ogawa reported that, as a result of analyzing the questionnaire survey no distinct differences were found in the definitions stated by respondents from several different schools. The standard images of “the local area” were the area where pains were felt when pressure is applied, the areas which transmit special sensitivity or the area where a disorder is found. Also, the images of “the distal area” were the areas which have some relationship to the local area, such as meridians and collaterals, nerves, reflex points, tender points, or related points which induce a clearly recognizable physical reaction.
Mr.Shinohara showed the superiority of distal area treatments according to the muscle meridian theory by utilizing the 3 arm crossover examination. Furthermore, he established the delayed myalgia as a model of the illness on a specific muscle meridian region. He investigated the effect of intradermal needling. He observed that after a weight bearing exercise on the biceps brachii, the threshold of tenderness on the Lung meridian decreases. He proposed the possibility of using the points in extremities which had selectively responded by manifesting of the delayed myalgia.
Mr. Moriyama, one of the minor speakes, explained, using the theory of biomechanics, the possibility of in-fluences of distal area disharmony on the local area. He introduced the Meridian Test as one clinically useful method for finding disharmony which is typically difficult to discern and may contribute to the disorder. He proposed that a microscopic “eye” to be used to see the local area and the macroscopic “eye” b used to see the whole body in medical examination and treatment. Using the “eyes” in synthesis and not separately, he concluded, must be the basic state for treatment.
Another minor speaker, Mr.Shiraishi reported on analyzed results of the experiments on lumbago patients. Applying Yuandaoci (distant needling) reduced a response on points of the trunk (BL 23, Shenshu, Jinyu) in stimulating points, i.e, BL 40 (Weizhong, Ichu), BL 57 (Chengshan, Shozan) BL 58 (Feiyang, Hiyo) BL 60 (Kunlun, Konron) GB 34 (Yanglingquan, Yoryosen) varying in many ways and confirming the peculiarity of each point, meridian and collateral. Based on these findings he supports the effectiveness of distal meridian area treatments.
Mr. Ozaki, also, another minor speaker proposed, from the outcome of animal experiments. Stimulation by acupuncture at either adjacent or distal areas can affect motor reflex. So from this point of view, insertion at adjacent and distal area has some recordable effect. He added that stimulation given either at adjacent or distal areas would converge to the same neuron and the same modification would occur. From this point of view, the difference in processing information at the neuron level would control the manifestation of the effect and thus investigations on these treatments should be discussed as a synthesis, including, not

[Purpose] This study investigated the safety of acupuncture and moxibustion in Japan. A questionnaire about infection control was developed based on the “Guidelines on basic training and safety in acupuncture” published by the World Health Organization (WHO) in 1999.
[Method] The questionnaire was sent to the members of the Japan Society of Acupuncture and Moxibustion (JSAM) and the Japan Acupuncture and Moxibustion Association (JAMA) in August 2000 and in September 2001, respectively. The items on the questionnaire were “a clean working environment”, “clean hands of the practitioner”, “preparation of the needling sites”, “sterile needles and equipment, and appropriate storage”, “aseptic technique” and “careful management and disposal of used needles and swabs”.
[Result&Conclusion] The questionnaire response rate was 49.6% (443/894). The results suggest that approaches to infection control are better than those in our previous report in 1996. However, the rate of using disposable needles was still low and most acupucturists held the needle with bare hands during inserting. It is necessary to improve pre-and postgraduate education regarding safety in performing acupuncture and moxibustion, including the infection control.

Moxibustion causes localized dermal vasodilation and increased capillary permeability, and this is expected to provide the clinical basis for the concept that Oriental medical herb therapies promote physiological activity in general. Hence, moxibustion has been carried out as one of the basic therapeutic methods in Oriental medicine. The aim of this study is to investigate the modulation effect of moxibustion on the local metabolism which was usually experienced as telangiectasia. This effect was evaluated by the hair growth rate and associated with histological findings of the hair root.
CH3/HeNCrJ mice with telogen hairs were used. The dorsal hair was shaved in an area approximately 8cm² at 6 weeks of age, and moxibustion stimulation was carried out at the center of the shaved area. The growth of the hair was examined by the hair length and the HE-stained subcutis preparation up to 11 weeks.
It was concluded that the vasodilation effect of moxibustion lasted for a fairly long period, and the number of hair follicle cells were increased by moxibustion.

Microstructure and elemental composition of dried Artemisa princeps Pamp. (Yomogi) leaves produced in Japan and China were investigated by scanning electron microscopy and X-ray microanalysis. Trichomes and T-foem hairs were found in higher density on the reverse side of the leaf of Yomogi. Cilium hairs were observed in Japanese Yomogi, while those in Chinese Yomogi, were distributed. Concentrations of K, Ca, Si and Cl were determined in plant fibers of the trichome in both Yomogi. In the head-style hairs of Japanese Yomogi, K, Ca, and Cl, S, P, Si were found, while K, Ca, Si, S, Mg and Fe were observed in the Chinese Yomogi.
These results suggest that there were differences in morphological and elemental composition between Japanese and Chinese dried Yomogi leaves. These differences will affect the moxa quality even after the purifying process.