Objective To study the content difference of avicularin,quercitrin and quercetin in Herba Taxilli from different host plants. Methods The contents of avicularin,quercitrin and quercetin in Herba Taxilli were determined by RP-HPLC and the samples were prepared by ultrasonic extraction with methanol. The analytical column was UltImate XB-C18(250 mm×4.6 mm,5μm), the mobile phase was acetonitrile-methanol-0.1%phosphate with gradient elution,at a flow rate of 1.0 ml/min. The column tempera-ture was set at 25℃,the detection wavelength was 254 nm for avicularin and quercitrin,and 365 nm for quercetin. Results The lin-ear range of the above three ingrediets were 0.0992-1.9840(r=0.9999),0.2254-4.4580(r=0.9999)and 0.1258-2.5160μg(r=0.9997) and the average recovery rates(n=5)were 98.39%,97.08%and 98.159%,respectively. Their contents from different host plants were 0.0000-0.0398,0.3977-0.7639 and 0.0068-0.0231 mg/g in branches and 0.0167-0.1704,1.8626-11.0041,and 0.0185-0.1841 mg/g in leaves. Conclusion The method is accurate,simple and reproducible,and can be used for the quality control of Herba Taxilli.