Objective To summarize the diagnosis and treatment of 25 cases of colonic cancer occurred after cholecystectomy ,and to explore the correlation between colonic cancer and cholecystectomy. Methods The clinical data of the 25 cases were retrospectively analysed. Results The onset time of the cancer was 10 years after cholecystectomy in 3 cases(12%) and over 12 years in 22 cases(88%). Six cases had family history of canceration(24%), 5 had colonic polyps(20%) and 2 had colonic adenoma (8%). Tumor located in appendix in 10 cases (40%),in ascending colonic in 7 cases (28%),in transverse colonic in 3 cases(12%),in descending colonic in 3 cases (12%) and in sigmoid colonic in 2 cases (8%). Eighteen patients underwent radical operation (72%),and 7 had palliative operation (28%). All patients recovered successfully after the operation. Conclusions Periodical postoperative follow up of the patients after cholecystectomy is an effective method to discover colonic cancer in early stage.