Objective:To evaluate the efficacy and safety of oral anisodine hydrobromide tablets in the treatment of nonarteritic anterior ischemic optic neuropathy (NAION).Methods:A multicenter nonrandomized controlled trial was conducted.A total of 282 acute NAION patients (282 eyes) were recruited from 16 hospitals in China from July 2020 to May 2021.Patients were divided into two groups according to treatment methods, which were control group (124 cases, 124 eyes) receiving regular treatment including citicoline sodium plus Ginkgo biloba leaf liquid extract or Ginkgo biloba leaf extract tablets plus mecobalamin, and experimental group (158 cases, 158 eyes) receiving treatment in control group plus oral anisodine hydrobromide tablets 1 mg, twice daily for 2 to 3 months.Best corrected visual acuity (BCVA), visual field index (VFI), peripapillary retinal nerve fiber layer (pRNFL) and radial peripapillary capillary vessel density (RPC) were assessed at 1, 2, 3, and 6 months after enrollment using the standard decimal visual acuity chart, 750i Humphery visual field analyzer, Cirrus HD-OCT 4000/Cirrus HD-OCT 5000, RTVue-XR optical coherence tomography respectively.The primary outcomes were BCVA and VFI, and the secondary outcomes were pRNFL, RPC, and the side effects during the follow-up.The study adhered to the Declaration of Helsinki.All patients were fully informed about the treatment and purpose of this study and voluntarily signed the informed consent form.The study protocol was approved by Chinese PLA General Hospital (No.S2020-021-01). Results:In all, 242 patients (242 eyes) completed the follow-up of BCVA, and 98 patients (98 eyes) completed the VFI follow-up.In terms of visual function, BCVA and VFI improved significantly over time in the two groups, and BCVA and VFI were better in experimental group than in control group at various follow-up time points (all at P<0.05). In terms of structure, pRNFL gradually decreased in both groups with the extension of treatment, and pRNFL was significanthy thinner in experimental group than in control group at various follow-up time points (all at P<0.05). There was no significant difference in RPC between the two groups at the last follow-up ( P>0.05). There were two cases with side effects and one case was discontinued due to side effects 25 days after enrollment. Conclusions:Oral anisodine hydrobromide can improve visual acuity and visual field in NAION and accelerate the regression of optic disc edema, with good safety.

The classification of lung tumor with the help of computer-aided diagnosis system is very important for the early diagnosis and treatment of malignant lung tumors. At present, the main research direction of lung tumor classification is the model fusion technology based on deep learning, which classifies the multiple fusion data of lung tumor with the help of radiomics. This paper summarizes the commonly used research algorithms for lung tumor classification, introduces concepts and technologies of machine learning, radiomics, deep learning and multiple data fusion, points out the existing problems and difficulties in the field of lung tumor classification, and looks forward to the development prospect and future research direction of lung tumor classification.

Objective To evaluate the effects of nutritional intervention on puerperal dietary behavior and postpartum recovery in puerperae. MethodsTotally 596 normal single uniparas who were pregnant for more than 28 weeks were selected and randomly divided into intervention (n=299) and control (n=297) groups. Patients in the intervention group received puerperal nutrition and healthcare knowledge training and individualized dietary guidance, while patients in the control group received routine puerperal nutrition and healthcare knowledge training. The intake of various food, recovery and complications during puerperium were compared between the two groups. ResultsThe intake of various food during puerperium in the intervention group was higher than that in the control group except for cereal grains and eggs (P＜0.05). The height of fundic uteri (7.2±3.2) cm, Visual Analogue Scale (VAS) score (2.64±1.10) and Body Mass Index (27.87±8.86) kg/m2 in the intervention group was lower than those in the control group 6 weeks postpartum, and there was statistically significant difference between the two groups (P＜0.05). The morbidity of puerperal infection was 1.3% in the control group, while that in the intervention group was 6.4%, and there was statistically significant difference between the two groups (χ2=10.28,P＜ 0.05). The rate of breast feeding was 84.3% in the intervention group, while that in the control group was 76.1%, and there was statistically significant difference between the two groups (χ2=6.30,P＜ 0.05). ConclusionsNutritional intervention can enable puerperae to choose an appropriate dietary structure and method during puerperium, improve their puerperal dietary behavior, accelerate rapid postpartum recovery, reduce puerperal complications, and improve the rate of breastfeeding.

Objective To explore the effects of dietary intervention on dietary behavior, dietary patterns, pregnancy weight gain control and prevention of adverse pregnancy outcome among singleton pregnant women. Methods From March 2017 to December 2017, a total of 1 000 primiparas with normal singleton pregnancy less than 13 weeks were randomly divided into intervention group and control group with the random number table, 500 cases in each group. Pregnant women of intervention group were received pregnancy diet nutrition health education and individualized nutrition guidance. Pregnant women of control group were treated with routine training on pregnancy nutrition and health care knowledge. The General Information Questionnaire, Nutrition Knowledge, Attitude, Practice (KAP) Questionnaire and the Case Report Form (CRF) were used to investigated related information in two groups, such as the population characteristics of sociology, pregnancy outcome, pregnancy complications, the score of KAP questionnaire and pregnancy weight gain. The Food Frequency Questionnaire (FFQ) was used to investigate the consumption of foods of pregnant women. Results A total of 986 pieces of valid data (494 in intervention group and 492 in control group) were collected in this study. The proportion of pregnant women with normal body mass index (BMI) and pregnancy weight gain in intervention group was higher than that in control group; the incidences of pregnancy complications, cesarean delivery, giant baby and premature baby of intervention group were lower than those of control group with significant differences (P＜0.05). There was no statistical difference in the score of KAP questionnaire of pregnant women between intervention group and control group during first-trimester (P＞0.05). The scores of KAP questionnaire of pregnant women during second-trimester and last trimester in intervention group were significantly higher than those in control group as well as those during first-trimester (P＜ 0.01). The consumption frequencies of foods of pregnant women in intervention group during last trimester were higher than those during first-trimester with significant differences (P＜ 0.05), except for the consumption frequency of "roughage and its products" (P＞0.05). Conclusions Pregnancy dietary intervention could help pregnant women to keep normal pregnancy weight gain, improve the level of KAP of pregnancy rational diet, better diet behavior and prevent pregnancy complications as well as adverse pregnancy outcome.

Objective To investigate the mental health status and related influence factors in the spouses of primipara, and to provide theoretical evidence of improving the quality of nursing care. Methods The symptom checklist 90 (SCL-90) was used to investigate the mental health status of primipara spouses (n=200) on the 42nd day after delivery. Results The participants had significantly higher scores of somatization (SOM), obsessive-compulsive(O-C), interpersonal sensitivity(I-S), depression (DEP), and anxiety (ANX) compared with the norms (t=13.87,3.34,8.75,7.57,6.49; P<0.01). Spouses without stable work had significantly higher scores of SOM, O-C, ANX, phobic anxiety (PHOB) and Psychoticism (PSY) compared with those with stable work (t=2.58,2.85,1.98,2.28,2.02; P< 0.05). Furthermore, the SCL-90 scores were significantly higher in those who had an unplanned pregnancy, an absence of prenatal training, short neonatal sleep time (<10 h), non-native residence in Beijing, and lived within stem families (P<0.05). Conclusions The mental health of the primipara spouses can be affected by the pregnancy and delivery, who should be included in the perinatal care program.

OBJECTIVETo study the expression and significance of tumor suppressor in lung cancer 1 (TSLC1) gene methylation, the expression of TSLC1 protein in cervix cancer and precancerous lesions as well as their relationship with HR-HPV DNA infection.

METHODSThe clinicopathological data of 92 cases of different cervical lesions during March 2011 to August 2012 treated in our hospital were collected. There were pathologically confirmed 10 cases of normal cervix, 26 cases of cervical intraepithelial neoplasia (CIN) I, 20 cases of CIN II, 15 cases of CIN III, and 21 cases of cervical cancer. Methylation-specific polymerase chain reaction (MSP) was used to detect the TSLC1 gene methylation status in cervical lesions, immunohistochemistry (SP) was used to detect the expressions of TSLC1 protein in cervical lesions, and the second generation hybrid capture (HC2) method was used to detect the high-risk HPV in cervical lesions.

RESULTSThe expression rate of TSLC1 gene methylation in normal cervical tissue, CIN I, CIN II, CIN III and SCC were 10.0%, 30.8%, 55.0%, 60.0%, 66.7%, respectively, showing a statistically significant difference (P = 0.004). The positive expression rate of TSLC1 protein in normal cervical tissue, CIN I, CIN II, CIN III and SCC were 100.0%, 80.8%, 65.0%, 33.3%, and 23.8%, respectively, with a significant difference (P = 0.004). In the progression from CIN to invasive cervical cancer, there was no significant correlation between TSLC1 gene methylation and HR-HPV DNA infection (P = 0.919), TSLC1 protein expression and HR-HPV DNA infection (P = 0.664). The correlation analysis showed a negative correlation between TSLC1 gene methylation and TSLC1 protein expression (r = -0.674, P < 0.001).

CONCLUSIONSTSLC1 gene promoter methylation may be an early event in the cervical carcinogenesis, become an early sensitive marker, and serve the early prevention and prognostic prediction for cervical cancer.

Cell Adhesion Molecule-1 ; Cell Adhesion Molecules ; genetics ; metabolism ; Cervical Intraepithelial Neoplasia ; genetics ; metabolism ; DNA Methylation ; Disease Progression ; Female ; Humans ; Immunoglobulins ; genetics ; metabolism ; Immunohistochemistry ; Methylation ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Uterine Cervical Neoplasms ; genetics ; metabolism

Objective To investigate the effect of plateau training on military operation ability ,evaluate the real situa-tion of combat forces on the plateau , and to provide data for effectively improving the combat effectiveness of the army stationed on the plateau .Methods According to the standards , the levels of high altitude acclimatization , physical work capacity (VO2max, PWC170) and mental work capacity (digit span, digit symbol, pursuit aiming and visual reaction time) were measured to assess the military operation ability of plateau training troops , including troops that entered the plateau from the plain(plateau-entering troops), troops stationed on the plateau (plateau troops) and troops stationed in the plain ( plain troops ) .Results The troops on the plateau all reached basic acclimatization .The aerobic capacities of these three types of troops were of the standard medium level , and the maximum oxygen uptake ( VO2max ) of the plain troops was greater than that of that of plateau-entering troops(P<0.01), and the VO2max of the plateau troops was greater than that of plateau-entering troops(P<0.01).Compared with the plain troops, the VO2 max and PWC170 of the plateau-entering troops decreased by 25.7%and 27.7%respectively.There was no significant difference in the digit span , digit symbol, pursuit aiming between these 3 kinds of troops , but the visual response of the plateau-entering troops was prolonged ( P<0.01), while the pursuit aiming of the plateau troops was lower than that of the plateau-entering troops(P <0.05). Conclusion Acclimatization-promoting measures and plateau training can contribute to high altitude acclimatization formation, but cannot overcome the objective adverse effects of the plateau on physical fitness .Two months of plateau training fails to improve physical fitness.Therefore, special attention should be paid to the research on scientific and effective measures to improve physical fitness on the plateau .

Objective To investigate and analyze the military operation ability and influence factors of soldiers alongthe border in an extremely cold environment and to provide a scientific basis for effectively improving the combat effective -ness of the army stationed the cold regions.Methods According to relevant standards, recruits(the length of service <1month) and old soldiers(the length of service >1 year) were randomly selected to investigate their military operationfactors, physical condition, sleep condition, mental health and cognitive ability.Results Cold was the main factor whichaffected military operation capability in cold regions.According to physical standards,border troops were in poor physicalcondition.Results of SCL-90 showed that the total score, the number of positive items , the score of each factor of the 130recruits were significantly lower than those of the Chinese army model and 46 veterans (P <0.05).There was nosignificant difference in these aspects between the 46 veterans and Chinese army model.Recruits were in better sleepcondition and had better instantaneous memory(P <0.05).Birthplace had no effect on these factors .Recruits from Centraland South China were in poorer sleep condition than those from cold regions (P <0.05).Results of SCL-90 showed that thetotal score,the factors of somatization, coercion, depression and anxiety of communication veterans were significantly higherthan those of the Chinese army model (P <0.05).The sleep condition of communication veterans was poorer than that ofmotorized infantry and patrol veterans (P <0.05).Conclusion Cold is the main factorthat impacts the ability of militaryoperations in cold regions.The physical work capacity of border guards in cold regions was significantly below the militarystandard, so the level of military training should be strengthened .Research on cold-protection equipment for special tasks should be strengthened.

Objective To investigate the effects of total alkaloids in Buxus microphylla leaves (ABML) on isolated rats thoracic aorta rings,and then to explore the possible mechanisms underlying the effects.MethodsThoracic aortas of Wistar rats were isolated,removed,and mounted onto an organ bath.The effects of ABML at different concentration on the contraction of isolated thoracic aorta rings (with and without endothelium) precontracted with KC1 or PE were observed with organ bath technique.Dose-effect curves of CaCl2 were recorded by organ bath technique.The concentration of intracellular Ca2+ ([Ca2+]i) increased by PE,KCI,and caffeine in the presence of ABML was determined using Ca2+ sensitive fluorescence indicator Fura-2/AM loaded thoracic aorta vascular smooth muscle (VSM) cells of rats.ResultsIn aorta rings precontracted with PE and KCI,ABML produced concentrationdependent relaxation in both intact and denuded endothelium ring groups.There was no difference in the inhibition of contraction between the intact and denuded endothelium ring groups at the same concentration.Exposure of isolated thoracic aorta rings to ABML led to a significant reduction in the contracting response induced by CaCI2,and shifted the cumulative concentration-response curves to right.ABML could significantly inhibit the extracellular Ca2+ influx induced by PE and KCI under [Ca2+]0 of 1.5 mmol/L,with inhibitory ratios of 40.2％ and 49.9％,respectively.In the case of Ca2+-free,ABML could significantly inhibit the intracellular Ca2+ release induced by PE,with inhibitory ratio of 72.4％.ConclusionABML relaxes thoracic aorta VSM cells by suppressing influx of extracellular Ca2+ via voltage-dependent Ca2+ channel and receptor-operated Ca2+ channel.

Aim To investigate the cardioprotective effects of Cyclovirobuxine D on pure cultared neonatal rat cardiomyoeytes exposed to hypoxia-reoxygenation (H/R) injury and the mechanisms. Methods The hypoxia/reoxygenation(H/R) injury model of pure cultured neonatal rat cardiomyocytes was developed.The concentration of lactate dehydrogenase (LDH) and malonialdehvde (MDA), the activity of super-oxide dismutes (SOD) and caspase-3, the apoptosis rate of cardiomyocyte were measured.Results Compared with the control group, the model group showed lower activity of SOD and higher concentrations of LDH , MDA, the apoptosis rate and activity of caspase-3(P