OBJECTIVE：To establish the method for content determination of 6 active ingredients in Paeonia lactiflora during different harvest periods ，and to investigate its variation rules so as to determine the optimal harvesting period. METHODS ：HPLC method was adopted to determine the contents of gallic acid ， catechin， alibiflorin， paeoniflorin， benzoic acid and benzoylpaeoniflorin，and principal component analysis was conducted . The determination was performed on Thermo C 18 column with mobile phase consisted of acetonitrile- 0.1％ phosphoric acid aqueous solution （gradient elution ）at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm，and column temperature was 35 ℃. The sample size was 10 µL. RESULTS ：The linear range of the above 6 ingredients were 0.013 2-0.25 mg/mL（r＝0.999 2），0.013 2-0.25 mg/mL（r＝0.999 9），0.026 8-0.51 mg/mL（r＝0.999 7）， 0.42-8.01 mg/mL（r＝0.999 2），0.016-0.31 mg/mL（r＝0.999 4），0.02-0.38 mg/mL（r＝0.999 8），respectively. The limits of quantification were 0.009 3，0.008 5，0.016 3，0.021 7，0.011 3，0.017 4 mg/mL，and the limits of detection were 0.003 3，0.002 7， 0.005 4，0.007 3，0.003 8，0.005 9 mg/mL. RSDs of precision ，stability and repeatability tests were less than 2％. The recoveries were 96.01％-99.43％（RSD＝1.23％，n＝9），97.95％-100.45％（RSD＝0.79％，n＝9），97.98％-100.11％（RSD＝0.68％，n＝ 9），98.83％ -100.09％ （RSD＝0.65％ ，n＝9），98.58％ - 100.95％（RSD＝1.35％，n＝9），96.28％-103.26％（RSD＝ 1.76％，n＝9）. The contents of above 6 ingredients in Radix Paeoniae Rubra （roots） were 0.016％ -0.057％ ，0-0.067％ ， 0.207％-0.640％，2.350％-5.887％，0.030％- 0.245％，0.054％- 0.381％，respectively. On May 30th，the drying rate of Radix 0451-87266873。E-mail：wangzhen_yue@163.com Paeoniae Rubra was the lowest （about 33％），and on Sept. 15th，the drying rate was the highest （about 49％）. The contents of gallic acid and paeoniflorin in the leaves of P. lactiflora were higher than the root during Jul.-Oct. Results of principal component analysis showed that the variance contribution rates of the first two principal components were 71.845％ and 18.170％，respectively；cumulative variance contribution rate was 90.015％. The months with higher comprehensive scores were May to Jun. and Sept. to Oct. CONCLUSIONS ：Established method is simple ， accurate，reproducible and precise. It can be used to determine the contents of 6 active ingredients in Radix Paeoniae Rubra during different harvest periods. Sept. 30th to Oct. 15th is the optimum harvesting periods for Radix Paeoniae Rubra ，and leaves can be harvested around Jul. 15th.

OBJECTIVE： To establish a determination method for content of polysaccharide in Acanthopanax senticosus， and to conduct cluster analysis and ultrasonic extraction technology optimization. METHODS： The content of polysaccharide in A. senticosus was determined by phenol-sulfuric acid method. Cluster analysis was conducted by using SPSS 23.0 software of the polysaccharide in A. senticosus from 17 habitats. The ultrasonic extraction technology was optimized with L9（34）orthogonal test using extraction temperature， the ratio of material to liquid， extraction time as factors， the content of polysaccharides as index， and then validated. RESULTS： The linear range of glucose ranged from 0.007 75 to 0.151 mg/mL （r＝0.999 1）； the limits of quantification and detection were 2.854， 0.856 μg/mL； RSDs of precision， stability， and repeatability tests were less than 2％； recovery rates of the sample were 98.41％-101.58％（RSD＝1.23％，n＝6）. Cluster analysis results showed that 17 batches of samples could be clustered into three classes； S1， S6， S10， S12 and S13 were clustered into one class； S3-S5 and S7 were clustered into one class； and the rest samples were clustered into one class. The optimal ultrasonic extraction technology was extraction temperature 55 ℃， ratio of material to liquid 1 ∶ 10 （g/mL）， extraction time 35 min. Results of validation tests showed the content of the polysaccharide under the best process condition was 4.36％（RSD＝0.920％，n＝3）. CONCLUSIONS： Established method is simple，reproducible and suitable for determination of polysaccharide in A. senticosus； the optimized ultrasonic extraction technology is stable and feasible.

Objective To explore the preliminary results of application of reserving the left subclavian artery by fenestration of domestic stent graft in the thoracic endovascular aortic repair (TEVAR) of type B dissection with unfavourable proximal landing zone.Methods From October 2015 to January 2018,a total of 13 patients with type B aortic dissection without healthy proximal landing zones underwent TEVAR using fenestration of domestic stent graft in our hospital.Computed tomography angiography (CTA) data were measured before surgery.Digital subtraction angiography (DSA) was conducted after surgery.All patients underwent CTA or ultrasound examination during follow-up.Operation success rate,survive rate,patency of left subclavian artery and complications were analyzed.Results All the patients were deployed fenestrated endografts successfully.No proximal endoleak happened during perioperative period.Delirium occurred in 1 case during 7 postoperative days and fully recovered with the medical treatment.The mean follow-up period was (9.8 ± 9.5) months (range,1-29 months).11 patients underwent CTA and 2 patients underwent ultrasound examination during follow-up.During period of following up,no patients died,no proximal endoleak appeared,and all the left subclavian arteries reserving by fenestration.Conclusions Reserving the left subclavian artery by fenestration of domestic stent graft in TEVAR of type B dissection with hostile proximal sealing zone is economical,safe and feasible,which expand the indication of TEVAR for aortic dissection,and the long-term outcomes of this technique need to be assessed in the future.

Objective To analyse the initial resucts in different treatment methods for the treatment of aortic arch injury.Methods A total of 23 patients (18 males and 5 females) with injuries of superior arch arteries underwent our treatment in Shandong Provincial Hospital Affiliated to Shandong University from January 2008 to May 2017.Nine patients underwent surgical procedures(surgical group),13 patients underwent endovascular procedures (endovascular group),1 patient underwent conservative treatment.The conditions of all survived patients were followed up by telephone or outpatient from one to sixty months postoperative.Mortality and complications such as rehaemorrhagia,cerebralinfarction,stenosis or blockage during the follow-up period was assessed.Operation time,hospitalization time after treatment,mortality and complications within 30 days of surgical group and endovascular group were compared by statistical methods.The data were analyzed by SPSS 16.0 software.Measurement data were expressed as ((x) ± s).The count data were expressed as frequency and percentage (％),and comparison between groups were evaluated with t test,Chi-square.Results All patients had achieved technical success.In patients treated by surgical procedures,one case of death and 2 cases of cerebralinfarction were reported within 30 days.One patient died of rehaemorrhagia during the 2nd day of open operation.Meanwhile,another two patients suffered from cerebral infarction,they recovered within 30 days.All patients treated by endovascular procedures survived without cerebralinfarction.However,blockage occurred in one patient of these patients.The operation time [(2.4 ± 1.1) hours and (1.0 ± 0.4) hours] (P ＜ 0.01) and the time of postoperative hospitalization [(8.3 ± 3.0) days and(2.5 ± 1.0) days] (P ＜ 0.05) were statistically different of the two groups.The postoperative mortality rate of the two groups undergoing open surgery and endovascular treatment within 30 days was 11.1％ and 0.0％ (P =0.41),and complication rates with 33.3％ and 7.7％ respectively (P =0.26),there has no significant difference.Overall,66.7％ (6/9) patients treated by surgical procedures and 92.3％ (12/13) patients treated by endovascular procedures and one patient treated by consewative treatment survived without any complication during the follow up period.Conclusions Initial results suggest that endovascular procedures can significantly shorten the time of operation and the time of postoperative hospitalization,and the mortality and complication rate are also slightly lower than surgical procedures.Endovascular procedures is an important method for treatment for injuries that involve superior arch arteries.It plays a special role in rescuing injuries of superior arch arteries.

Objective:To obtain AP2/ERF genes from Brassica oleracea by using in silico cloning. Methods: AP2/ERF genes were cloned by retnieving the EST database and using the bioinformatics software with the Arabidopsis thaliana AP2/ERF as a querying probe. Results:Two AP2/ERF family transcriptional regulators (BoAP2/ERF1 and BoAP2/ERF2) were isolated from Brassica olera-cea by the in silico cloning method. Some characters of AP2/ERF protein were analyzed and predicted by the tools of bioinformatics in the following aspects including the composition of amino acid sequence, hydrophilicity and hydrophobicity, subcellular localization, secondary and tertiary structure of protein and function. Conclusion:Bioinformatical analysis shows BoAP2/ERF1 and BoAP2/ERF2 gene encode 40. 85kDa and 39. 44kDa protein with 371 and 352 amino acids. The domain is predicted to locate on nucleus. Sequence analysis indicates the protein may be involved in signaling transducer and stressing response roles in plantbiotic stresses.

OBJECTIVE:To determine the content of 17 amino acid in sarcocarp of northeast Empetrum nigrum simultaneous-ly. METHODS:HPLC-ELSD method was adopted. The determination was performed on Waters Symmetry C18 column with mobile phase consisted of 5.0 mmol/L heptafluorobutyric acid solution(containing 0.7% trifluoroacetic acid)-acetonitrile(gradient elution) at the flow rate of 2.0 L/min,the column temperature was 37 ℃,the drift tube temperature was set at 60 ℃. RESULTS:The line-ar ranges of glycine,serine,aminosuccinic acid,aminopropionic acid,threonine,glutamic acid,cystinol,diaminocaproic acid, histidine,arginine,proline,valine,methionine,oxyphenylaminopropionic acid,isoleucine,aminocaproic acid,phenylalanine were 0.0059-0.1877,0.0082-0.2628,0.0104-0.3328,0.0070-0.2227,0.0093-0.2978,0.0115-0.3678,0.0094-0.3004,0.0114-0.3655,0.0121-0.3880,0.0136-0.4355,0.0090-0.2878,0.0092-0.2930,0.0117-0.3730,0.0142-0.4530,0.0103-0.3280, 0.0103-0.3280 and 0.0129-0.4130 mg/mL(all r>0.9990). RSDs of precision,stability and repeatability tests were not all less than 2.0%. Recoveries were 98.0%-101.2%(RSD<2.0%,n=6). CONCLUSIONS:This method is simple,precision,stable and repeatable,and can be used for simultaneous determination of 17 amino acid in sarcocarp of northeast E. nigrum.

The bacterium Streptomyces peucetius produces doxorubicin and daunorubicin,and the two widely used anticancer antibiotics.Two open reading frames,drrA and drrB,were proposed to encode for an ABC (ATP-binding cassette) type of permease that carries out export of the antibiotic ABC (ATP binding cassette)-type transporter for the exportation of these two antibiotics.In this paper,the structure,expression and interaction of DrrA and DrrB protein and the research progress of the assembly and multi drug efflux function of DrrAB are briefly reviewed.

Objective To investigate the effects of spine region of interest (ROI),tracking range,and real-time image contrast ratio on the positioning errors of cyberknife.Methods The LTT dynamic phantom was used to develop a spine tracking plan and perform treatment,and the target positioning system was used to preset the phantom and obtain real-time preset image and positioning error.Based on the realtime preset image,spine ROI,tracking range,and real-time image contrast ratio were changed to observe the changes in positioning error and related parameters.Pearson correlation analysis was performed.Results The change in tracking range did not change the positioning error in spine tracking,and tracking range was not correlated with positioning error (R =0,P =1).The changes in ROI and image contrast ratio did not affect the translation error,but affected the rotation error,especially the rotation error in left-right direction (r =0.533 and 0.693,P=0.002 and 0.026).The image contrast ratio had the most obvious effect,with an amplitude of variation up to 2.2°.Conclusions The change in tracking range does not affect the positioning errors in spinal tracking,but the changes in ROI and image contrast ratio can cause varying degrees of changes in positioning errors,which should be taken seriously in cyberknife treatment.

The numerous noxious reactions caused by misusing medicinal plant ingredients have been widely concerned at home and abroad. The problems of safe drug use needed to be solved. DNA barcode is a powerful molecular identification method, which can cover the shortage of tradditional morphological and chemical identification. In recent years, DNA barcode provided new aspects for the identification of Chinese herbal medicines and have obtained outstanding achievement. Several popular DNA regions ( ITS, ITS2, ps-bA-trnH, rbcL and matK) have been used for the identification of Chinese herbs.

Objective To analyze the thin-section CT features of fissure-attached nodules in asymptomatic patients.Methods The baseline and follow-up CT images of 120 asymptomatic patients with fissure-attached nodules were retrospectively analyzed.The thin-section CT findings include size,shape,density,interface,and the relationship with pulmonary fissure.Results There were 1 67 fissure-attached nodules of the 120 patients.The mean length of nodules was 5.46 mm±2.276 mm (2 - 1 5 mm).104(104/1 67,61.1%)nodules were less than 5 mm in length.1 63 (1 63/1 67,97.6%)nodules were typical and 4 (4/1 67,2.4%)nodules were atypical.All these nodules manifested as homogenous,solid,smooth interface,and widely adjacent to the fissure on CT ima-ges.Among those nodules,1 64(1 64/1 67,98.2%)nodules were triangular(88/1 67,52.7%)or oval(76/1 67,45.5%).126(126/1 67, 75.4%)nodules were abutting the major fissure.The angle between nodule and fissure was obtuse in 1 64(1 64/1 67,98.2%)fissure-attached nodules.During follow-up,4(4/1 67,2.4%)nodules increased in size and the corresponding volume doubling time (VDT) range was 863-4 228 days.However,no obvious malignant signs were found.Conclusion The thin-section CT images of fissure-attached nodules in asymptomatic patients showed the benign characteristics,most of which less than 5 mm and demonstrated trian-gle or oval,smooth interface and homogenous solid density.