Objective To study anti-aging mechanism of skin with water extract of Codonopsis Radix by applying computational biology and animal experiments.Methods A total of 50 SPF 8-week-old C57BL/6 mice were selected and then randomly divided into five groups.D-galactose-induced aging mice model was constructed,and different doses of water extract of Codonopsis Radix were used for intervention.Hematoxylin-eosin staining(HE)and Masson staining were used to observe the pathological changes of mouse skin tissue.The content of hydroxyproline(HYP),superoxide dismutase(SOD),and malondialdehyde(MDA)in mouse skin were measured by biochemical detection.Transmission electron microscopy was used to observe the ultrastructural changes of mouse skin tissue.The chip data of skin aging from GEO database was obtained to screen skin-aging differential genes.TCMSP and UniProt databases were used to search for active ingredients and targets of Codonopsis Radix.The intersection of targets for Codonopsis Radix-skin aging was obtained by integrating the above data.A protein interaction network of all core gene proteins for Codonopsis Radix intervention in skin aging was constructed through the STRING database.Then,quantitative real-time PCR(RT-qPCR)and Western Blotting were used to verify target genes expression and pathway-related protein expression after the intervention of Codonopsis Radix in the aging model.Results Compared with the normal control group,the skin tissue structure of mice in senile model group were damaged significantly,the damage of skin tissue structure was improved significantly after the intervention of Codonopsis Radix.Compared with the normal control group,the content of HYP and SOD in the skin tissue of mice in senile model group were significantly reduced(P＜0.05),while MDA was significantly increased(P＜0.05).After the intervention of Codonopsis Radix,the content of HYP and SOD were increased,while MDA was decreased(P＜0.05)compared with senile model group.It was found that matrix metalloproteinase 9(MMP9)was the core target for the intervention of Codonopsis Radix on skin aging in computational biology.Experiments have shown that the expression of MMP9 was significantly increased in the skin of aging model mice compared to normal control group(P＜0.05).After the intervention of Codonopsis Radix,the expression of MMP9 is significantly reduced(P＜0.05),the expression of the key protein including inhibitory subunit of NF-kappa B alpha(IκBα)、IκB kinase-alpha(IKKα)、nuclear factor kappa-B(NF-κB)P65 of NF-κB signaling pathway were significantly changed(P＜0.05).Conclusion Codonopsis Radix water extract can effectively alleviate skin aging in aging model mice by inhibiting the protein expression of IκBα、IKKα、NF-κB P65 of NF-κB signaling pathway,reducing the expression of downstream gene MMP9,and ultimately alleviate skin collagen damage and resist skin aging.

Objective To explore the experiences and needs of patients making health behavior decisions after percutaneous coronary intervention(PCI),and to provide evidence for formulating targeted decision support strategies.Methods Based on the Ottawa decision support framework and descriptive qualitative study,16 patients with unhealthy behaviors 1 year after PCI were selected as research subjects in a tertiary A general hospital in Harbin from July to December 2022 by purpose sampling method,and their decisional experience and needs were deeply interviewed by semi-structured interview method.The data were sorted out and analyzed by content analysis method.Results After the analysis of the interview data,the 2 themes and 6 sub-themes were extracted,including the experience of health behavior decision(uncertainty about the benefits of health behavior,low recognition of health behavior,and worry about the negative impact of behavior change);health behavior decision-making needs(health behavior knowledge needs,medical resources needs,emotional support needs).Conclusion PCI patients have a variety of conflicting experiences and multi-dimensional health behavior decision-making needs.Clinical nurses should start from the experience and needs of patients,pay attention to and identify the conflict experience of patients early,meet the individual needs of patients from the aspects of knowledge,medical resources,emotional support,etc.,and promote the transformation and long-term maintenance of PCI patients'health behaviors.

0n December 31,2021,the Chinese Nursing Association released the group standard"nursing care of patients with infusion of vasoactive agents(T/CNAS 22-2021)",which outlines the fundamental requirements for intravenous infusion of vasoactive drugs and standardizes the evaluation,administration,and monitoring.This article provides an interpretation of the key parts and sections of the standard to ensure nursing safety during the administration of vasoactive drugs,aiming to reduce complications.Additionally,it serves as a crucial reference for nurses during the administration of the medication.

Objective:To investigate the status quo and influencing factors of the somatization of depressive emotions among college students, in order to provide a theoretical basis for the reform of mental health education and the formulation of operable mental health counseling for college students.Methods:Totally 297 college students from two institutions of higher education in Heilongjiang Province were selected by convenience sampling and investigated with the College Student Depressive Emotion Somatization Questionnaire, Attitudes Toward Seeking Professional Psychological Help Scale-Short Form (ATSPPH-SF), and the Type D Personality Scale (DS14) .Results:The total score for the College Student Depressive Emotion Somatization Questionnaire among the 297 students was (28.49±8.07), and the total score for the ATSPPH-SF was (21.98±2.73). Multiple linear regression analysis showed that the influencing factors for the somatization of depressive emotions in college students were professional psychological help-seeking attitudes and whether they had a Type D personality ( P < 0.05) . Conclusions:During university education, there should be a focus on students' personality traits and the positivity of their attitudes toward seeking professional psychological help in order to mitigate the occurrence of adverse psychological issues such as symptoms of depressive somatization.

ObjectiveTo systematically evaluate the efficacy and safety of Tianmeng Oral Liquid in the treatment of hyperprolactinemia caused by antipsychotics in female patients with schizophrenia， and to provide references for intervention of antipsychotic-induced hyperprolactinemia patients. MethodsEnglish databases （PubMed， Cochrane Library， PsycINFO and Embase） and Chinese databases （CNKI and Wanfang） were systematically searched， with a search time frame of the period from database creation to September 16， 2022. Randomized controlled studies （RCTs） on adjunctive Tianmeng Oral Liquid in the treatment of hyperprolactinemia caused by antipsychotics in female patients with schizophrenia were included. Three investigators independently screened the literatures and extracted data according to PICOS principles and evaluated the quality of including literatures， and the Meta-analysis was performed by using RevMan 5.3. ResultsA total of 3 articles including 256 female schizophrenic patients with hyperprolactinemia were included. Meta-analysis results showed that at the treatment endpoint， the improvement of hyperprolactinemia symptoms reported a significant superiority of the intervention group than the control group （RR=1.73， 95% CI： 1.07~2.79， P˂0.05）. Similarly， the significant superiority of the intervention group than the control group were also found in reducing serum prolactin levels （WMD=-55.17， 95% CI： -68.16~-42.18， P<0.01） and Positive and Negative Syndrome Scale （PANSS） total scores were lower in the intervention group than in the control group （WMD=-7.36， 95% CI： -8.94~-5.77， P<0.01）. ConclusionTianmeng Oral Liquid may help improve clinical efficacy and reduce serum prolactin levels in female schizophrenia patients with hyperprolactinemia. It may improve psychiatric symptoms in female patients with schizophrenia.

Objective:To explore the effect of network learning environment on critical thinking ability of nursing undergraduates.Methods:From September 2021 to January 2022, a total of 84 nursing undergraduates in the third grade of Nursing School of Harbin Medical University were selected as the research objects. The Online Learning Environment Scale and the Chinese version of Critical Thinking Ability Scale were used to investigate.Results:The total score of Online Learning Environment Scale of 84 nursing undergraduates was (63.21±15.29) , and the Chinese version of Crital Thinking Ability Scale was (248.71±36.35) . The total score of Online Learning Environment Scale of nursing undergraduates was positively correlated with the total score of Chinese version of Critical Thinking Ability Scale of nursing undergraduates ( P<0.01) . The results of multiple linear regression analysis showed that the influencing factors of critical thinking ability of nursing undergraduates included family economic situation, whether they could receive free online database resources from schools, and online learning environment ( P<0.05) . Conclusions:The online learning environment for nursing undergraduates is at a moderate level. It is suggested that nursing undergraduates should optimize the content and form of online learning and provide targeted learning support resources so as to improve the critical thinking ability of nursing undergraduates.

Objective:To explore the effect of online and offline blended scenario simulation teaching in the practice of nursing undergraduates.Methods:From August 2020 to May 2021, convenience sampling was used to select 76 nursing undergraduates in the class of 2017 who practiced in the Cardiology Department of the Second Affiliated Hospital of Harbin Medical University as the research object. The undergraduates were randomly divided into the experimental group and the control group, 38 in each group. The control group received the traditional graduation practice training, and the experimental group received online and offline blended scenario simulation teaching on this basis. After the practice, the clinical thinking ability of the two groups of students was evaluated by the Clinical Thinking Ability Evaluation Scale, the clinical practice ability of the two groups of students was evaluated by the Objective Structured Clinical Examination (OSCE) , and a teaching satisfaction survey was conducted.Results:The total score of clinical thinking ability, critical thinking ability, systematic thinking ability, evidence-based thinking ability, OSCE total score of the students in the experimental group were higher than those in the control group, and the differences were statistically significant ( P<0.05) . The students in the experimental group thought that the teaching method was helpful for in-depth understanding and application of theoretical knowledge, improving nursing assessment skills, clinical coping skills and communication skills, and at the same time helping to cultivate critical thinking and teamwork awareness, and arouse learning enthusiasm, and the differences were statistically significant (all P<0.05) . Conclusions:The online and offline blended scenario simulation teaching can effectively improve the clinical thinking ability, clinical practice ability and teaching satisfaction of nursing undergraduates, and it has good effect in the graduation practice of undergraduate nursing students.

Objective:To explore the predictive effect of acute stress disorder (ASD) in patients with acute myocardial infarction (AMI) on subsequent post-traumatic stress disorder (PTSD) , and to clarify the potential mediating role and pathway of type D personality and cognitive evaluation in the evolution of ASD to PTSD in AMI patients.Methods:Using the convenient sampling method, patients with newly diagnosed AMI in Department of Cardiology of the 2nd Affiliated Hospital of Harbin Medical University from September to December 2019 were selected as the research subjects. General Information Questionnaire, Acute Stress Disorder Scale (ASDS) , Type D Personality Scale-14 (DS-14) and Cognitive Appraisal of Health Scale (CAHS) were used for survey 1 to 2 days after hospitalization.One month after discharge from the hospital, a follow-up call was used to complete Post-traumatic Stress Disorder Checklist-Civilian Version (PCL-C) .Results:A total of 494 questionnaires were distributed in this study, and 457 valid questionnaires were returned.The incidence of ASD in AMI patients was 31.29% (143/457) , and the ASDS score was (40.28±12.74) . The incidence of PTSD was 33.5% (153/457) , and the PCL-C score was (34.49±14.00) .The moderated mediating effect analysis showed that ASD could directly and positively predict PTSD ( P<0.01) , and injury cognition and irrelevant cognition played a mediating role in the transition from ASD to PTSD ( P<0.01) .At the same time, type D personality can influence the process of ASD to PTSD transformation by regulating injury cognition and irrelevant cognition ( P<0.05) . Conclusions:The occurrence of ASD in AMI patients can effectively predict the occurrence of PTSD one month after discharge.At the same time, the cognitive evaluation of type D personality regulation can play an important mediating role in the process of ASD transforming into PTSD.

Objective:To explore the effects of mindfulness-based stress reduction training (MBSRT) on anxiety and depression levels and sleep quality of clinical nurses.Methods:Totally 90 clinical nurses from the Second Hospital of Harbin Medical University were selected by convenient sampling from April 2018 to June 2019, and were divided into experimental and control groups according to the order of enrollment, with 45 cases in each group. Nurses in the control group received routine sleep and emotional guidance, while nurses in the experimental group underwent MBSR training on this basis. Self-Anxiety Scale (SAS) , Self-Depression Scale (SDS) , and Pittsburgh Sleep Quality Index (PSQI) were used to evaluate the intervention effect in both groups 1 day before the intervention and the day when the intervention was completed. Finally, 44 nurses in the experiment group and 45 in the control group completed the study.Results:Before the MBSRT intervention, there was no statistically significant difference in the SAS, SDS, and PSQI scores between the two groups of nurses ( P>0.05) . After the intervention, the SAS and SDS scores of nurses in the experimental group were lower than those of the control group, and the differences were statistically significant ( t=-3.117, -3.498; P<0.05) ; the total PSQI score and scores in such dimensions as subjective sleep quality, sleep latency, sleep continuity, sleep efficiency, sleep disorder, hypnotic drug use, and daytime dysfunction in the experimental group were lower than those of the control group, and all differences were statistically significant ( P<0.05) . Conclusions:MBSRT can effectively reduce the level of anxiety and depression and improve the sleep quality of clinical nurses.

Objective:To investigate the acute stress disorder (ASD) , post-traumatic stress disorder (PTSD) , anxiety, and depression in patients with acute myocardial infarction (AMI) , and analyze the predictive effect of ASD, anxiety, and depression during hospitalization on the PTSD, anxiety and depression in the following month.Methods:From September to December 2019, convenience sampling was used to select 457 AMI patients from a ClassⅢ Grade A hospital in Harbin as the research object. The Acute Stress Disorder Scale (ASDS) , Posttraumatic Stress Disorder Checklist-Civilian Version (PCL-C) , Beck Anxiety Inventory (BAI) and Beck Depression Inventory-Ⅱ (BDI-Ⅱ) was used to investigate the patients during hospitalization (within one week of admission) and the following month.Results:Among 457 AMI patients, the incidence of ASD, anxiety and depression during hospitalization were 31.29% (143/457) , 42.23% (193/457) and 29.98% (137/457) , respectively. The incidence of PTSD, anxiety, and depression in AMI patients at one month was 30.20% (138/457) , 10.50% (48/457) , and 26.48% (121/457) , respectively. The results of the cross-lagged analysis showed that ASD (β=0.11, P<0.05) , anxiety (β=0.16, P<0.01) , depression (β=0.25, P<0.01) during hospitalization had statistical significance in predicting PTSD at one month. Besides, anxiety during hospitalization had statistical significance in predicting anxiety (β=0.34, P<0.01) and PTSD (β=0.16, P<0.01) at one month, and depression during hospitalization had statistical significance in predicting depression (β =0.59, P<0.01) and PTSD (β=0.25, P<0.01) at one month. Multiple linear regression analysis showed that the high alertness, re-experience and separation of symptoms of ASD had statistical significance on the predictive effect of PTSD ( P<0.01) . Conclusions:ASD, anxiety, and depression during hospitalization of AMI patients have a positive predictive effect on PTSD, anxiety, and depression in the following month. Psychological intervention during hospitalization of AMI patients should be strengthened to relieve their anxiety, depression, fear and other negative emotions in time, so as to effectively reduce the occurrence of later traumatic stress disorder.