Objective To evaluate the molluscicidal effect of suspension concentrate of niclosamide ethanolamine salt(SC-NE)against Oncomelania hupensis snails in laboratory and field. Methods The experiment of SCNE against the snails by using the immersing and spraying methods was performed in laboratory and field,with control groups of wettable powder of ni-closamide ethanolamine salt(WPN). Results In the laboratory,LC50(s) of SCNE for 24,48 h and 72 h by using the immersion method were 0.0926,0.0629 mg/L and 0.0549 mg/L,respectively. The mortality rates of snails for 24,48 h and 72 h by using the immersion method were all 100％ with the concentrations of 0.25 mg/L. The mortality rates of snails were all 100％ while spraying SCNE for 3 d in the laboratory with the concentrations of 0.25 g/m2. In Jiangling County,except 0.5 g/m3 SCNE immers-ing the snails for 24 h,the mortality rates of snails by using SCNE with the immersing method were all 100％. While the concen-tration of SCNE was 0.5 g/m3 or above,the mortality rates were all 100％after the use of it with the immersion method for 2 d in Gong'an County. In Jiangling County,the mortality rates of snails by using SCNE 0.5 g/m3 for 1 d,3 d,and 7 d with the spray-ing method were 87.5％,92.82％and 97.40％respectively. While the concentration of SCNE was 0.5 g/m3,the mortality rates were 85.94％,86.78％and 94.21％respectively after the use of it with the spraying method for 1 d,3 d,7 d in Gong'an Coun-ty,and the molluscicidal effect of SCNE(1.0 g/m2)was higher than that of WPN. Conclusion SCNE has a high molluscicidal effect in the laboratory and field,and it is a novel and simple formulation of niclosamide.

Objective To study the expression of Gult-1 in cervical adenocarcinoma and prognosis analysis.Methods Gult-1 was detected by immunohistochemistry (EnVision) in cervical adenocarcinoma and cancer adjacent tissue.Results In cancer adjacent tissue and cervical adenocarcinoma,the positive rates of Gult-1 were 2.22 ％ (1/45),58.67 ％ (44/75),respectively,and there was statistical significance between them (x2 =38.23,P =0.00).The expression of Gult-1 was not correlated to age,histological classification and infiltrated depth (all P ＞ 0.05).However,it was correlated to tumor size,histological grade and lymphatic nodes metastasis (all P ＜ 0.05).The survival rate of positive Gult-1 patients was lower than negative Gult-1 patients (x 2 =4.27,P =0.04).Conclusion The over-expression of Guh-1 in cervical adenocarcinoma indicates poorly differentiated cancer,the possibility of lymphatic nodes metastasis and unfavourable prognosis.

Objective To understand the schistosomiasis endemic situation at a national surveillance site in Gong an Coun-ty Hubei Province. Methods According to The Scheme of Schistosomiasis Surveillance in China the schistosome infection rates of residents and cattle and the Oncomelaniahupensissnail status were investigated yearly in Zhangjiahu Village a nation-al surveillance site in Gong an County from 2004 to 2013. Results From 2004 to 2013 the human schistosome infection rates decreased from 10.66%to 0.58% and the cattle schistosome infection rates from 12.75%to 0. Meanwhile the snail areas and densities reduced and the schistosome infected snails were eliminated. Conclusion The schistosomiasis endemic situa-tion declined dramatically at the surveillance site and schistosomiasis is well-controlled.

Objective To investigate the expressions of Wnt1 and β-catenin proteins in mammary cancer and its relationship with clinical pathological characteristics,and explore its role in the pathogenesis of breast cancer.Methods Immunoshistochemisty (SP method) was used to detect the expressions of Wnt1 and β-catenin proteins in adjacent tissue of breast cancer (n =15),breast hyperplasia tissues (n =25),and breast in vasive ductal carcinoma (n =80).SPSS 17.0 statistical software was used for analysis,such as count data with x2 test and correlation analysis with Spearman test,with significance level of α =0.05.Results The positive rate of Wnt1 and β-catenin in breast cancer group [62.5％ (50/80) and 68.8 (55/80)] was significantly higher than that in hyperplasia group [28％ (7/25) and 20％ (5/25)]and in borderline group [13.33 (2/15) and 13.33 (2/15)] with a significant difference (P ＜ 0.01).Positive expression of Wnt1 and abnormal expression of β-catenin in breast invasive ductal carcinoma were related to lymphatic metastasis (x2 =5.10,4.87,P ＜ 0.05).Abnormal expression of β-catenin was also related to the histological grading of breast invasive ductal carcinoma (x2 =5.61,P ＜ 0.05).The expressions of Wnt1 and β-catenin proteins in invasive ductal carcinoma showed a positive correlation (r =0.313,P ＜ 0.01).Conclusions The high level expression of Wnt1 protein and the abnormal expression of β-catenin protein might play an important role in breast carcinogenesis,and was related to lymph node metastasis.

ObjectiveTo investigate the expression of Twist protein in adenocarcinoma of prostate and clinicopathological features and explore its roles in the carcinogenesis and progression of adenocarcinoma of prostate and its relationship with prognosis.MethodsThe expression of Twist was detected in 70 cases with adenocarcinoma of prostate and 30 cases with side cancer tissue by immunohistochemistry (EnlivionTM).ResultsThe expression of Twist protein in adenocarcinoma of prostate was higher than side cancer tissue.The expression of twist was related to Gleason grade,clinical stage and bony metastasis (P＜0.05),but it was not related to the age (P＞0.05).The survival rate of Twist protein positive patients was significant lower than negative patients(x2=9.52,P＜0.01).ConclusionsTwist might be an important biological marker for malignant transformation and invasion and metastasis adenocarcinoma of prostate,and the up-regulation of the expression of Twist reflects more invasion and poor prognosis.

ObjectiveTo observe the expression of Twist protein, vascular endothelial growth factor (VEGF) mRNA and protein in laryngeal squamous cell carcinoma(LSCC) and their relation to clinical pathological characteristics, and aslo debate their effect and clinical application in carcinogenesis, developing of LSCC. MethodMolecular hybridization in situ was carried to detect VEGF mRNA, and immunohistochemistry S-P method was carried to detect the expression of Twist protein and VEGF protein in 80 cases of LSCC, 30 cases of paraneoplastic tissue. ResultsThe expression of Twist protein, VEGF mRNA and protein was related to TNM stage, T stage, thyroid cartilage involvement, lymph node metastasis and distance metastasis (P ＜ 0.05 or ＜ 0.01 ). The expression of Twist protein and VEGF protein was related to differentiated degree (P ＜ 0.01 or ＜ 0.05), however, the expression of VEGF mRNA was not related to differentiated degree (P ＞ 0.05 ). The expression of Twist protein was positively related to the expression of VEGF protein in LSCC (r =0.334, P ＜ 0.01 ) ; and the expression of VEGF mRNA was positive correlation to the expression of VEGF protein (r =0.484,P ＜ 0.01 ). ConclusionsOverexpression of Twist protein and VEGF mRNA and protein might play a joint action in the carcinogenesis, development of LSCC. Combined detection of Twist protein and VEGF mRNA and protein has vital significance in predicting the invasion and metastasis of LSCC, which might be an index to judge the biological behavior of LSCC.

Objective To investigate the expressions of Snail mRNA and E-cad mRNA in invasive ductal carcinoma and their clinical significance. Methods The expression of Snail mRNA and E-cad mRNA were detected on mammary gland hyperplasia (30cases), intraductal breast carcinoma(30cases) and invasive ductal carcinoma (70cases)by in situ hybridization. Results The positive expression rate of Snail mRNA and E-cad mRNA in mammary gland hyperplasia, intraductal breast carcinoma and invasive ductal carcinoma were 23. 3% ,46. 7% ,81.4% and 96. 7% ,66. 7% ,35.7% ,respectively. There was significant difference among them(P ＜ 0. 01). There was difference between Snail mRNA and E-cad mRNA in the same group(P ＜0. 05). Snail mRNA was not related to age, tumor size or histopathological grade (P ＞0. 05) ,but it was related to lymphatic metastasis (P ＜0. 01). E-cad mRNA was not related to age, tumor size(P ＞0. 05),but it was related to lymphatic metastasis and histopathological grade(P ＜0. 01). There were positive relationship between Snail mRNA and E-cad mRNA(r = -0. 56, P =0. 00). Conclusions The overexpression of Snail mRNA and low expression of E-cad mRNA were involved in the infiltration and metastasis of breast carcinoma, and they were related to lymphatic metastasis. Therefore, the test of the expression of them were valuable in predicting the prognostic and metastasis of breast carcinoma.

0 05). CD 34 , CD 31 and F VIII of the average counts of microvessels was 69 5?15 7, 65 3?14 5 and 58 3?16 3 in the NSCLC with metastasis and 44 3?14 3, 40 5?12 6 and 36 5?15 8 in those without metastasis showing a significant difference (P

Objective To investigate the effect of antigen retrieval by microwave and the expression of CD 44 s and CD 44v6 in human intracranial metastastic tumor and human glioblastomas, and the relationship between the expression of CD 44 adhesion molecules and the invasion or metastasis of these tumors.Methods After antigen retrieval by microwave-0.1%Triton X-100(MW-T) and buffer solution of microwave-citrate salts(MW-CA), CD 44 s and CD 44v6 were used to detect the expression of CD 44 adhesion molecules in 20 normal human brain tissues, 35 human glioblastomas, and 30 intracranial metastastic tumor by microwave-LSAB immunohistochemical staining technique.Results Integral optical density(IOD) value of CD 44 s and CD 44v6 positive cells restored by MW-T and MW-CA was higher significantly than that of without antigen retrieval (P

0 05).CD 34 , CD 31 and F VIII of the average counts of microvessels were (69 5?15 7), (65 3?14 5) and(58 3?16 3) respectively in the NSCLC with metastasis and (44 3?14 3), (40 5?12 6) and (36 5?15 8) respectively in those without metastasis,there was a significant difference (P