Objective To investigate the correlation between Yes-associated protein(YAP)nuclear expression and tumor size with prognosis of patients with epithelial ovarian cancer(EOC)and to study the role of YAP in EOC.Methods 120 patients with EOC were selected as the experimental group,including 38 patients with early stage(Ⅰ+Ⅱ)EOC and 8 2 patients with advanced stage(Ⅲ+Ⅳ)EOC.3 0 normal ovarian tissues obtained from patients with uterine leiomyoma were enrolled as the control group.Immunohistochemical(IHC)assay was em-ployed to determine YAP expression and sub-location.The relationship between YAP expression and the pathologi-cal parameters of the 120 patients with EOC was analyzed,so as to the prognosis of these patients.EOC cells(C13K and OV2008)were cultured with varying initial cell volumes.Ki67 expression and cell proliferation were tested by immunofluorescence and cloning assay respectively.YAP expression at mRNA and protein levels were de-tected by q-PCR and Western blot respectively when the cell conference of EOC cells reached to low(60％)and high(90％)cell density.Results The YAP nuclear expression was significantly higher in the EOC group com-pared to the control group(P＜0.05).The average diameter of stage Ⅰ+Ⅱ EOC was larger than that of stage Ⅲ+Ⅳ EOC(P＜0.01).The high nuclear expression of YAP was positively associated with pathological grade,clinical stage and the level of Ca125＞1 000 IU/ml,while negatively correlated with tumor size(all P＜0.05).Survival analyses showed that smaller tumor size(＜10 cm)and higher YAP nuclear expression were negatively as-sociated with the 3-year overall survival rate of EOC patients(P＜0.01).C13K and OV2008 cells cultured in the low density group exhibited a high number of clone formation,high Ki67 and YAP expression(P＜0.01).The down-regulation of YAP expression could decrease the cell viability of EOC cells in the low-and high-density groups(P＜0.05).Conclusion Higher level of YAP nuclear expression and smaller tumour size are inversely associated with the clinical prognosis of patients with EOC.Inhibiting YAP nuclear expression leads to a decrease in the prolif-eration capacity of EOC cells.

Objective To analyze the current status of medication therapy management(MTM)against the background of"Internet+"in China,to reveal its research hotspots and development trend through visual methods,and to provide a firm reference for promoting innovative pharmaceutical development and the transformation of pharmacists.Methods Using CiteSpace 6.2 R2,368 Chinese studies from the CNKI,CBM,and VIP databases were collected and analyzed.Relevant graphs were drawn,and the results were analyzed through post-trend,cooccurrence,cluster,and burst analysis.Results The number of articles issued in China's"Internet+"MTM field is on the rise.However,the cooperation network between authors and research institutions is relatively scattered.The research team led by tertiary hospitals has played an essential role in this field,but the medical consortium has not fully utilized its advantages.In addition,informatization and pharmacists are the research objects of continuous concern,while quality of life and diabetes are recent research hotspots.Conclusion"Internet+"MTM is a new medical service model involving multiple disciplines and fields.In this paper,CiteSpace 6.2 R2 performed a visual analysis of the literature on"Internet+"medication therapy management in China,revealing the research status,concerns,and development trends in this field,which has specific reference value for relevant policy formulation and research.

Objective:To explore the interleukin-31 protein expression in the hypertrophic scar of incision tissue after surgery and its underlying pathological impact.Methods:From February 2022 to February 2023, three HS patients scar tissue (HS) and their normal skin tissue (Control, NS) were obtained. Two patients were female and one patient was male. The tissues were fixed in 4% formalin and embedded in paraffin. Haematoxylin-eosin (HE) stain and immunohistochemical stain were used to evaluate the epidermal thickness, myofibroblasts of dermis and the expression level of IL-31 between HS and NS.Results:The epidermis thickness was (303.88±46.03) μm in HS group, while (133.02±17.40) μm in NS group ( t=12.60, P<0.001). The expression level of IL-31 protein was measured by IRS score and positive cell density. The IRS score was 9.89±2.03 of the basal layer in HS group and was 4.33±1.66 of the basal layer in NS group. The positive cell density was 786 343.83±159 627.97 of the basal layer in HS group ( P<0.001) and was 555 457.61±128 097.21 of the basal layer in NS group ( P=0.014). In the dermis layer, the IRS score was 7.11±1.05 in HS group and was 4.33±0.71 in NS group, the positive cell density was 156 760.97±26 046.10 in HS group ( P<0.001) and was 49 576.01±52 369.33 in NS group ( P<0.001). In the dermis layer, the count of myofibroblasts was 120.44±15.75 in HS group while was 27.39±14.89 in NS group ( t=23.79, P<0.001). Conclusions:Our study demonstrates that both myofibroblast count and IL-31 protein expression level are notably increased in HS patients. The expression of IL-31 protein is prominent in the cytoplasm of myofibroblasts, basal cells, macrophages and mast cells which could implicate that IL-31 may be a potential therapeutic target to enhance the resolution of HS.

Objective To evaluate the clinical value of ultrasonography in the diagnosis of carotid plaque neovascularization .Methods 32 patients with carotid artery plaques diagnosed by conventional ultrasonography were enrolled.The patients were followed up for contrast-enhanced ultrasonography (CEUS) and ultrasonography micro-vascular imaging(SMI),and the results of the two-dimensional detection of carotid plaque and plaque neovasculariza-tion were compared .Results A total of 63 plaques were detected by SMI , including 39 hypoechoic plaques , 19 episodes of echo patches ,58 of plaques ,and 17 hypoechoic plaques .The diagnostic rate of neovascularization was 10.34％.Among the 113 carotid plaques,48 carotid plaques were detected by SMI ,46 carotid plaques were detected by CEUS.The distribution of neovascularization was similar to that of CEUS ,and the neovascularization of SMI and CEUS was better(Kappa=0.669,P<0.001).Conclusion For the diagnosis of carotid plaques ,ultrasonography can accurately detect the number , location and neovascularization of plaques , and the accuracy of carotid plaques is effective.It provides a radiographic basis for the diagnosis and early intervention of patients ,which has great signifi-cance to the prevention of stroke and cardiovascular and cerebrovascular adverse events .It is worthy of popularizing and application .

Objective: To analyze the compatibility taboo of polyene phosphatidyl choline injection with several kinds of commonly used clinical drugs.Methods: The medical literatures on the compatibility taboos of polyene phosphatidyl choline injection with several commonly used drugs were retrieved and statistically analyzed.Results: After the compatibility of polyene phosphatidyl choline injection with vitamin C, raceanisodamine injection, doxofylline and sodium chloride injection,ambroxol hydrochloride injection,ondansetron hydrochloride injection and the other commonly used drugs, physical and chemical reactions happened in varying degrees.Conclusion: Polyene phosphatidyl choline injection has compatibility taboos with a variety of commonly used drugs.

As a main cellular organelle for bioenergy production , the mitochondrion plays a pivotal role in aerobic respiration , substance metabolism , oxidative stress , apoptosis and calcium homeostasis .Increasingly studies have shown a close relationship between mitochondrial dysfunction and cancer .Mitochondrial metabolic disturbance , reactive oxygen species ( ROS ) increase, mitochondrial gene mutation , calcium overload and abnormal apoptosis can influence tumorigenesis , growth, invasiveness and metastasis of multiple tumors .We aimed to summarize the mechanisms and influences of mitochondrial dysfunction on cancer .

Objective To explore the effect of simvastatin on the proliferation,apoptosis and protein expressions of keloid fibroblasts under normoxia,hypoxia or TGF-β1 treatment.Methods Keloid fibroblasts (KFs) were isolated by explants culture method.KFs were treated with different concentrations of simvastatin under normoxia or hypoxia (2％ O2) for 24 h and 48 h.The effects of simvastatin on cell proliferation were detected by CCK-8.Flow cytometer was used to detect the apoptosis of KFs treated with 10 μ mol/L simvastatin for 24 h or 48 h under normoxia,hypoxia or 10 ng/ml TGF-β1 treatment.Then the expressions of keloid-related proteins were analyzed by Western Blot.Results It showed that simvastatin could inhibit the proliferation of KFs in a concentration-and time-dependent manner with the concentration range of 10-500 μ mol/L for 24 h and 0.1-500 μ mol/L for 48 h.This inhibitory effect could be significantly enhanced when cells were incubated under hypoxia for 48h with 10-500 μ mol/L simvastatin.10 μ mol/L simvastatin could not influence the apoptosis of KFs under normoxia or TGF-β1 treatment,neither incubated for 24 h nor 48 h.When incubated under hypoxia,10 μ mol/L simvastatin could significantly induce the apoptosis of KFs,with the rate of 155.6％ for 24 h and 478.8％ for 48 h,compared with no-drug control.There are no significant influences on the expression of type Ⅰ collagen,CTGF or TIMP-1 when KFs were treated with 10 μ mol/L simvastatin under normoxia for 48 h.When incubated with 10 ng/ml TGF-β1 together with 10 μmol/L simvastatin for 48 h,the expression of CTGF was significantly inhibited.KFs treated with 10 μ mol/L simvastatin under hypoxia for 48 h showed a significant decrease of type Ⅰ collagen and CTGF,and a significant increase of TIMP-1.Conclusions Simvastatin has different effects on the proliferation,apoptosis and protein expressions of KFs in a dosedependent manner under different conditions.The effects are enhanced under hypoxia.

Objective To compare the differences of mitochondrial functions between keloid fibroblasts and normal skin fibroblasts and explore its relationship with cell proliferation.Methods Keloid fibroblasts (KFb) and normal skin fibroblasts (NFb) were isolated by explants culture method.KFb and NFb were cultured under normoxia or hypoxia (2％ O2).Differences of cell proliferation were detected by CCK-8.Flow cytometer was used to detect the content of mitochondria and reactive oxygen species (ROS) in KFb and NFb.Ultra-structures of mitochondria in KFb and NFb were observed by transmission electron microscope (TEM).Mitochondria fusion/fission related genes MFN1,MFN2 and FIS1 were detected by RT-PCR.Oxygen consumption rate,lactate production and ATP contents were determined by spectrophotometry.Results KFb showed a higher proliferation rate compared with NFb,especially under hypoxia.The oxygen consumption rate,ATP content,lactate production and ROS of KFb were lower than NFb under normoxia.After incubated under hypoxia,there was a significant increase in oxygen consumption,ATP content,lactate production and ROS in KFb,while NFb showed less increase compared with KFb.KFb had 15.33％ more mitochondrion than NFb,and expressions of MFN1,MFN2,FIS1 in KFb were 33.27％,113.39％ and 20.34％ higher compared with NFb.Under TEM,KFb showed an increase of enlarged mitochondrion,with disrupted inner membrane and loss of cristae.Conclusions KFb may have dysfunctions of mitochondrion which lead to changes of cell metabolism and continuous proliferation of KFb.

Objective To explore the effect of simvastatin on the proliferation,apoptosis and protein expressions of keloid fibroblasts under normoxia,hypoxia or TGF-β1 treatment.Methods Keloid fibroblasts (KFs) were isolated by explants culture method.KFs were treated with different concentrations of simvastatin under normoxia or hypoxia (2％ O2) for 24 h and 48 h.The effects of simvastatin on cell proliferation were detected by CCK-8.Flow cytometer was used to detect the apoptosis of KFs treated with 10 μ mol/L simvastatin for 24 h or 48 h under normoxia,hypoxia or 10 ng/ml TGF-β1 treatment.Then the expressions of keloid-related proteins were analyzed by Western Blot.Results It showed that simvastatin could inhibit the proliferation of KFs in a concentration-and time-dependent manner with the concentration range of 10-500 μ mol/L for 24 h and 0.1-500 μ mol/L for 48 h.This inhibitory effect could be significantly enhanced when cells were incubated under hypoxia for 48h with 10-500 μ mol/L simvastatin.10 μ mol/L simvastatin could not influence the apoptosis of KFs under normoxia or TGF-β1 treatment,neither incubated for 24 h nor 48 h.When incubated under hypoxia,10 μ mol/L simvastatin could significantly induce the apoptosis of KFs,with the rate of 155.6％ for 24 h and 478.8％ for 48 h,compared with no-drug control.There are no significant influences on the expression of type Ⅰ collagen,CTGF or TIMP-1 when KFs were treated with 10 μ mol/L simvastatin under normoxia for 48 h.When incubated with 10 ng/ml TGF-β1 together with 10 μmol/L simvastatin for 48 h,the expression of CTGF was significantly inhibited.KFs treated with 10 μ mol/L simvastatin under hypoxia for 48 h showed a significant decrease of type Ⅰ collagen and CTGF,and a significant increase of TIMP-1.Conclusions Simvastatin has different effects on the proliferation,apoptosis and protein expressions of KFs in a dosedependent manner under different conditions.The effects are enhanced under hypoxia.

Objective To compare the differences of mitochondrial functions between keloid fibroblasts and normal skin fibroblasts and explore its relationship with cell proliferation.Methods Keloid fibroblasts (KFb) and normal skin fibroblasts (NFb) were isolated by explants culture method.KFb and NFb were cultured under normoxia or hypoxia (2％ O2).Differences of cell proliferation were detected by CCK-8.Flow cytometer was used to detect the content of mitochondria and reactive oxygen species (ROS) in KFb and NFb.Ultra-structures of mitochondria in KFb and NFb were observed by transmission electron microscope (TEM).Mitochondria fusion/fission related genes MFN1,MFN2 and FIS1 were detected by RT-PCR.Oxygen consumption rate,lactate production and ATP contents were determined by spectrophotometry.Results KFb showed a higher proliferation rate compared with NFb,especially under hypoxia.The oxygen consumption rate,ATP content,lactate production and ROS of KFb were lower than NFb under normoxia.After incubated under hypoxia,there was a significant increase in oxygen consumption,ATP content,lactate production and ROS in KFb,while NFb showed less increase compared with KFb.KFb had 15.33％ more mitochondrion than NFb,and expressions of MFN1,MFN2,FIS1 in KFb were 33.27％,113.39％ and 20.34％ higher compared with NFb.Under TEM,KFb showed an increase of enlarged mitochondrion,with disrupted inner membrane and loss of cristae.Conclusions KFb may have dysfunctions of mitochondrion which lead to changes of cell metabolism and continuous proliferation of KFb.