Objective To evaluate the clinical use of the baseline CT angiography(CTA)quantitative score(self-designed collateral circulation quantitative,SD-CCQ)in determining the collateral circulation compensation status in patients with acute ischemic stroke(AIS),as well as the reliability and accuracy of the SD-CCQ score and the Alberta Stroke Program Early CT Score(ASPECTS).Methods Retrospective analysis was made on the clinical and imaging data,including CT,CTA and DWI image data,of 84 patients who were admitted for acute ischemic stroke to the Department of Neurorehabilitation of Zhongshan Hospital of Traditional Chinese Medicine from January 2020 to December 2022.Their CTA source images were annotated using a multi-task deep learning method for vascular segmentation.The ASPECTS score and SD-CCQ score were then applied to the CTA images following vascular segmentation in order to assess the collateral circulation compensation of AIS patients.The Kappa test was used to assess the consistency of the two methods used to assess collateral circulation,and the multifactorial Logistic regression analysis was used to examine the relationship between the SD-CCQ and the prognosis of the AIS patients.Results ASPECTS score had good consistency with SD-CCQ score in evaluating collateral circulation in AIS patients(κ=0.65,P＜0.001),and the diagnostic accuracy of the latter for benign collateral circulation in AIS was 96.15％.Logistic regression analysis showed that the new collateral circulation score,baseline NIHSS,and DWI infarct volume were the main factors affecting the long-term prognosis of AIS patients.Conclusion The new scoring system SD-CCQ can be used to evaluate the compensatory status of collateral circulation in AIS patients,which may help in clinical treatment decision-making and prognosis prediction.

Objective To establish a scientifically rigorous,standardized,and comprehensive evaluation index system for the emergency response competency of'Internet+'nurses,and to provide references for the access,training and assessment of'Internet+'nurses.Methods Between November 2022 and July 2023,a draft of the evaluation index system was developed based on theoretical analysis,literature reviews and semi-structured interviews.Delphi expert consultation and analytic hierarchy process were used to determine the evaluation index system for emergency response competence of'Internet+'nurses.Results The response rates of 2 rounds were 90.48％and 100％;the opinion rates were 63.16％and 10.53％;the authority coefficients of the experts were 0.855 and 0.850;the Kendall's coefficients of concordance were 0.175 and 0.182.Finally,the evaluation index system for emergency response competency of'Internet+'nurses was formed,which consists of 4 first-level indexes(including prevention capacity,preparedness capacity,response capacity and recovery capacity),13 second-level indexes and 40 third-level indexes.Conclusion The evaluation index system for the emergency response competency of'Internet+'nurses is highly scientific and practical,which has guiding significance for the access,training and assessment of'Internet+'nurses.

Objective To compare the dose difference of intensity-modulated radiotherapy (IMRT) with different gantry angle between target volumes and surrounding normal tissues in lumbar spinal metastases therapy. Methods Ten patients with lumbar spinal metastases were enrolled in the study. Three plans with the same prescription dose of 3000 cGy/10f were designed by seven-equal beams (plan-A), five-back beams (plan-B) and seven-back beams (plan-C). All the plans were designed with the same objective function and dose limiting condition. The difference of dosimetric parameters of planning target volume (PTV), organ at risk (OAR), normal tissues, and treatment parameters in all the plans were compared with SPSS 21.0 statistical software. Results All the plans satisfied the clinical requirement. There was no significant difference in the D_mean, D_2%, D_98%, CI and HI of PTV between plan-A and plan-C (P >0.05), and these parameters of plan-A and plan-C were better than plan-B (P < 0.05). Compared with the other two plans, plan-B reduced the dosimetric parameters of bilateral kidney (P < 0.05), whereas plan-B increased the D_max of spinal cord (P < 0.05). The V₅ and V₁₀ of normal tissue of plan-B were lower than the other two plans while the V₁₅, V₂₀ and V₂₅ showed inverse relationship (P < 0.05). plan-B had certain advantages in shortening the monitor units and treatment time (P < 0.05).. Conclusions The seven-equal beams (plan-A) and seven-back beams (plan-C) IMRT plans can provide better target dose distribution, and reduce the D_max of spinal cord. Five-back beams (plan-B) IMRT plan had certain advantages in protecting bilateral kidney and shortening treatment time.

Objective:To investigate the changes of nitric oxide, endothelin-1 (ET-1), homocysteine (Hcy), C-reactive protein (CRP) levels in patients with obstructive sleep apnea hypopnea syndrome (OSAHS) combined with hypertension and the relationship between the changes and polysomnography monitoring indicators.Methods:A retrospective case-control study was conducted to analyze the clinical data of 115 patients with OSAHS complicated with hypertension, 110 patients with simple OSAHS and 136 healthy persons in Kailuan general hospital and Linxi Hospital of Kailuan general hospital from March 2016 to March 2018. The relationship between sleep related indexes and serological indexes was compared among the three groups.Results:In the control group, simple OSAHS group, OSAHS with hypertension group, the apnea hypopnea index (AHI) was (2.4±0.8), (35.7±12.1), (46.8±13.9) times/h, the lowest oxygen saturation(LSaO 2) was (87.1±6.8) %, (77.8±6.5) %, (66.9±5.1) %, and the mean oxygen saturation(MSpO 2) was (92.7±6.9) %, (80.9±6.7) %, (71.4±6.3) %, and the longest apnea time (LAT) was(4.5±0.7), (48.6±11.3), (58.2±10.4) s. The difference between the three groups was statistically significant ( F values were 637.92, 329.49, 322.96, 1 420.03, respectively, all P<0.05). Nitric oxide in control group, simple OSAHS group and OSAHS combined with hypertension group were (72.8±8.8), (53.7±7.4), (42.6±5.9) μmol/L.Endothelin-1 was(31.1±6.5), (45.4±6.7), (56.6±7.8) ng/L.Hcy was (8.7±1.9), (15.9±6.7), (31.4±6.3) μmol/L.CRP was (1.8±0.6), (18.6±4.3), (25.2±6.4) mg/L.The difference between the three groups was statistically significant ( F valves were 517.46, 419.02, 594.40, 982.14, respectively, all P<0.05). Pearson correlation analysis showed that the levels of endothelin 1, Hcy and CRP in OSAHS patients with hypertension were positively correlated with AHI and LAT (r values were 0.525, 0.704, 0.578, 0.434, 0.464 and 0.449, respectively; all P<0.05), and negatively correlated with LSaO 2 and MSpO 2 (r values were-0.477, -0.458, -0.429, -0.517, -0.534 and -0.617, respectively; all P<0.05). Conclusion:In OSAHS patients with hypertension, nitric oxide decreased, ET-1, Hcy, CRP increased, and polysomnography monitoring indicators were closely related to endothelin-1, Hcy, CRP.

Echinostoma revolutum is a zoonotic food-borne intestinal trematode that can cause intestinal bleeding, enteritis, and diarrhea in human and birds. To identify a suspected E. revolutum trematode from a red-crowned crane (Grus japonensis) and to reveal the genetic characteristics of its mitochondrial (mt) genome, the internal transcribed spacer (ITS) and complete mt genome sequence of this trematode were amplified. The results identified the trematode as E. revolutum. Its entire mt genome sequence was 15,714 bp in length, including 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and one non-coding region (NCR), with 61.73% A+T base content and a significant AT preference. The length of the 22 tRNA genes ranged from 59 bp to 70 bp, and their secondary structure showed the typical cloverleaf and D-loop structure. The length of the large subunit of rRNA (rrnL) and the small subunit of rRNA (rrnS) gene was 1,011 bp and 742 bp, respectively. Phylogenetic trees showed that E. revolutum and E. miyagawai clustered together, belonging to Echinostomatidae with Hypoderaeum conoideum. This study may enrich the mitochondrial gene database of Echinostoma trematodes and provide valuable data for studying the molecular identification and phylogeny of some digenean trematodes.

Objective:To assess the effectiveness of peer-supported diabetes self-management group intervention and explore a community-based diabetes health management model.Methods:From September to December in 2016, patients with type 2 diabetes were recruited from 6 streets (townships) in Binhu and Xishan district of Wuxi, Jiangsu Province. According to a structured diabetes self-management course, guidance was provided by community health workers and peer volunteers. Self-management group intervention was led by type 2 diabetes patients. A self-control design was used to collect information on self-management behavior, fasting blood glucose, and health care utilization at baseline and after 4-month intervention, and compare the difference of these indicators before and after the intervention.Results:Compared with the baseline, 147 participants aged from 30 to 80 years old had more increases in proportions of salt control (78.2% vs 60.5%, P<0.001) and oil control (79.6% vs 58.5%, P<0.001) in daily diet. The P50 ( P25, P75) of scores of symptom management and consultation list increased from 6 (0, 11) and 0 (0, 2) to 9 (4, 13) and 1 (0, 2) ( P<0.05) after 4-month intervention. Fasting blood glucose significantly decreased from (8.8±2.9) mmol/L at baseline to (7.6±1.9) mmol/L and the control rate of blood glucose significantly increased from 29.3% to 44.2% ( P<0.05) after 4-month intervention. The proportion of outpatient visited decreased from 42.2% to 12.9% and the proportion of hospitalized decreased from 10.2% to 4.1% ( P<0.05) after 4-month intervention. Conclusion:Peer-supported diabetes self-management group intervention could effectively improve behavior and blood glucose control of patients with type 2 diabetes.

Objective:To assess the effectiveness of peer-supported diabetes self-management group intervention and explore a community-based diabetes health management model.Methods:From September to December in 2016, patients with type 2 diabetes were recruited from 6 streets (townships) in Binhu and Xishan district of Wuxi, Jiangsu Province. According to a structured diabetes self-management course, guidance was provided by community health workers and peer volunteers. Self-management group intervention was led by type 2 diabetes patients. A self-control design was used to collect information on self-management behavior, fasting blood glucose, and health care utilization at baseline and after 4-month intervention, and compare the difference of these indicators before and after the intervention.Results:Compared with the baseline, 147 participants aged from 30 to 80 years old had more increases in proportions of salt control (78.2% vs 60.5%, P<0.001) and oil control (79.6% vs 58.5%, P<0.001) in daily diet. The P50 ( P25, P75) of scores of symptom management and consultation list increased from 6 (0, 11) and 0 (0, 2) to 9 (4, 13) and 1 (0, 2) ( P<0.05) after 4-month intervention. Fasting blood glucose significantly decreased from (8.8±2.9) mmol/L at baseline to (7.6±1.9) mmol/L and the control rate of blood glucose significantly increased from 29.3% to 44.2% ( P<0.05) after 4-month intervention. The proportion of outpatient visited decreased from 42.2% to 12.9% and the proportion of hospitalized decreased from 10.2% to 4.1% ( P<0.05) after 4-month intervention. Conclusion:Peer-supported diabetes self-management group intervention could effectively improve behavior and blood glucose control of patients with type 2 diabetes.

Recently, liposomes have been widely used in cancer therapeutics, but their anti-tumor effects are suboptimal due to limited tumor penetration. To solve this problem, researchers have made significant efforts to optimize liposomal diameters and potentials, but little attention has been paid to liposomal membrane rigidity. Herein, we sought to demonstrate the effects of cholesterol-tuned liposomal membrane rigidity on tumor penetration and anti-tumor effects. In this study, liposomes composed of hydrogenated soybean phospholipids (HSPC), 1,2-distearoyl--glycero-3-phosphoethanolamine--[methoxy(polyethylene glycol)-2000] (DSPE-PEG) and different concentrations of cholesterol were prepared. It was revealed that liposomal membrane rigidity decreased with the addition of cholesterol. Moderate cholesterol content conferred excellent diffusivity to liposomes in simulated diffusion medium, while excessive cholesterol limited the diffusion process. We concluded that the differences of the diffusion rates likely stemmed from the alterations in liposomal membrane rigidity, with moderate rigidity leading to improved diffusion. Next, the tumor penetration and the anti-tumor effects were analyzed. The results showed that liposomes with moderate rigidity gained excellent tumor penetration and enhanced anti-tumor effects. These findings illustrate a feasible and effective way to improve tumor penetration and therapeutic efficacy of liposomes by changing the cholesterol content, and highlight the importance of liposomal membrane rigidity.

Objective: To evaluate the efficacy and safety of low dose sublingual nifedipine dripping pills (5 mg) in treating moderate and severe hypertension in comparison with normal dose (10 mg) of sublingual nifedipine dripping pills. Methods: This study was designed as a randomized, double-blind, positive drug parallel controlled, multi-center, non-inferiority clinical trial. Patients with moderate and severe hypertension were enrolled by 14 clinical trial centers, randomly divided into the trial group (sublingual 5 mg nifedipine dripping pills) and the control group (sublingual 10 mg nifedipine dripping pills). The changes in blood pressure were monitored continuously within 2 hours after the initial administration, repeated the dose in 20 minutes interval after the initial administration for up to additional 3 doses (maximum 4 doses) if the antihypertensive efficacy was not satisfactory. The efficacy of antihypertensive therapy between the two groups was evaluated by repeated administration rates and blood pressure changes at 60 minutes post the initial administration, and the safety of treatment was evaluated by recording adverse event rate of the two groups. Results: The anti-hypertensive effective rates at 60 minutes after sublingual administration were 83.5% (202/242) and 86.7% (208/240) respectively between the trial group and control group (χ²=1.307, P=0.253) . On the aspect of antihypertensive effectiveness at 60 minutes after single dose of sublingual administration, the anti-hypertension effective rates of the trial group and the control group were 85.6% (154/180) and 87.2% (164/188) respectively (χ²=0.221, P=0.639). Prevalence of the repeated administration was also similar between the two groups (25.6%(62/242) in the trial group and 21.7% (52/240) in the control group, χ²=1.043, P=0.307). On the safety aspect, there was no adverse events/reactions in the trial group, but there were 15 cases of adverse events/reactions occurred in control group (6.25%, χ²=15.611, P<0.001). Conclusions In the treatment of moderate to severe hypertension, the antihypertensive efficacy of low dose nifedipine dripping pills is similar to that of conventional dosage, and the safety profile of low dose nifedipine dripping pills is better than that of the conventional dose.

Melting temperature shift (T(m)-shift) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a T(m)-shift method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5′ end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of T(m)-shift was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The T(m)-shift method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of T(m)-values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was 5.22×10⁻⁶ and 5.28×10⁻⁶ ng/μl samples of AceP and AtuP, respectively. The accuracy of T(m)-shift method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the T(m)-shift detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.
Ancylostoma ; Ancylostomatoidea ; Animals ; Cats ; DNA ; Freezing ; Methods ; Plasmids ; Polymorphism, Single Nucleotide ; Real-Time Polymerase Chain Reaction ; Tail