【Objective】 To investigate the clinical effects of electrophysiological technique combined with pelvic floor muscle training on urinary incontinence after electroresection of benign prostatic hyperplasia (BPH) based on the concept of enhanced recovery after surgery (ERAS). 【Methods】 A total of 86 patients with urinary incontinence treated with electroresection of BPH during Oct.2019 and Feb.2022 were randomly divided into control group and observation group, with 43 cases in either group. All patients received health education plus pelvic floor muscle training, and the observation group also received electrophysiological treatment. The differences in the indexes before and after treatment were compared between the two groups. 【Results】 There were no significant differences in the 1 h pad test, ICIQ-SF score, maximum urinary flow rate (Qmax), post void residual (PVR), International Prostate Symptom Score (IPSS), 24 h urinary incontinence frequency, and Urinary Incontinence Quality of Life Questionnaire Score (I-QoL) before treatment between the two groups (P>0.05). However, after treatment, Qmax and I-QoL score were significantly higher in the observation group than in the control group, while the other 5 indicators were significantly lower in the observation group than in the control group (all P<0.05). The total clinical effective rate was significantly higher in the observation group than in the control group (P<0.05). 【Conclusion】 Electrophysiological technique combined with pelvic floor muscle training can effectively improve the symptoms of urinary incontinence and promote the recovery of urinary control function, which is in line with the concept of ERAS and is worthy of further research and promotion.

AIM: To compare the early response to the new and traditional antiepileptic drugs (AEDs) in the treatment of partial epilepsy. METHODS: Patients from neurology Department of Huzhou Central Hospital between January 2013 and June 2018 were included; outcomes included time to first seizure, time to treatment failure and 6-month, 1- and 2-year seizure-free rates were compared. RESULTS: A total of 250 patients with partial epilepsy were divided into carbamazepine (CBZ) group (n =62), levetiracetam (LEV) group (n = 67), oxcarbazepine (OXC) group (n = 63), and lamotrigine (LTG) group (n = 58). In terms of time to first seizure after monotherapy, CBZ and OXC were equivalent (P = 0.635), while CBZ was superi- or to LTG (P < 0.001) and LEV (P = 0.005); regarding time to treatment failure, CBZ and LTG had the same response (P = 0.721), while CBZ was superior to OXC and LEV (P = 0.008 and P = 0.018, respectively). For the "6-month seizure-free" rate, differences were not statistically significant. For the " 1 - and 2-year seizure-free" rates, CBZ > LTG > OXC > LEV, and CBZ was superior to OXC and LEV (all P < 0.05), but not LTG (P > 0.05). A total of 25 patients had adverse reactions; with CBZ (19.3%) more often than LTG (8.6%), OXC (7.9%), or LEV (4.5%). CONCLUSION: Treatment response to CBZ is superior compared to that of OXC and LEV, especially in the early stages of treatment, and equivalent to that of LTG, but the incidence of side effects is higher as well.

Objective To establish and optimize a loop-mediated isothermal amplification (LAMP) method for the rapid detection of Escherichia coli and its microbial toxin.Methods The LAMP reaction system and reaction conditions were determined by optimizing LAMP reaction,and the optimized LAMP system was used for the detection.Results Primers targeting shiga toxin (stx) gene and O157 antigen gene rfbe were designed.The established and optimized LAMP amplification system contained 1.2 mmol/L dNTPs,10 mmol/L MgSO4,0.4 mol/L betaine,1 μl 10 × Bst DNA polymerase Buffer,8 U Bst DNA polymerase fragment,2 μl DNA template,and the ratio of inner-primer (FIP and BIP) and outerprimer (F3 and B3) were 8∶ 1.Time and temperature for LAMP was 60 min,60 ℃.The sensitivity was 103 times higher than polymerase chain reaction (PCR),reached 5 × 101 CFU/ml.When LAMP was applied to 19 reference strains,102 EHEC strains,the specification was 100％ while identification rate of rfbe,stx1 and stx2 gene reached 100％,95.2％,92.9％.Conclusions The LAMP method showed a promising prospect for the rapid detection of common nosocomial pathogens microbial toxin.

Objective To investigate the effect of pramipexole in the treatment of Parkinson after stroke .Methods 94 cases with Parkinson after stroke in our hospital from June 2013 to November 2015 were randomly selected and divided into two groups,47 cases respectively.Control group received routine etiological and symptomatic treatment, the study group received conventional therapy and symptomatic treatment and pramipexole treatment,two groups were treated for eight weeks.Parkinson’s disease scores,inflammatory cytokines and oxidative stress index,and the clinical effect and complications of contrast were compared after the treatment.Results Compared with before treatment,scores of daily activities of emotion integral integral,the motor function in two groups decreased,levels of serum IL-1β,IL-6,TNF-αand Hs-CRP decreased,levels of CAT,T-SOD,T-GSH in plasma increased (P<0.05),and compared with the control group,scores of daily activities of emotion integral integral,the motor function in the study group were lower,levels of serum IL-1β,IL-6,TNF-αand Hs-CRP were lower,levels of CAT,T-SOD,T-GSH in plasma were higher (P<0.05),and the total effective rate in the study group 91.49% was higher than the control group 74.47% (P<0.05).Conclusion Pramipexole in the treatment of Parkinson after stroke was effective with high safety,and it can reduce Inflammatory factors and increase oxidative stress.

To retrospectively analyze the clinical data of 176 patients of recurrent cerebral infarction at our hospital.Based upon the treatment of acute stroke trial (TOAST) classification,the types of recurrent ischemic stroke with initial brainstem infarction were classified.And univariate and multivariate logistic analyses of risk factors were performed.The major types in a decreasing order were atherosclerotic cerebral infarction (AT,n =24,70.6％),small arterial occlusive stroke (SA,n =8,23.5％) and cardiogenic cerebral embolism (CE,n =2,5.9％).Brainstem infarction was more likely to be affected by poor life habits and a history of diabetes mellitus (DM).And atrial fibrillation was a major independent risk factor for non-brainstem infarction.Multivariate logistic analysis showed that poor living habits (P =0.03),a history of DM (P =0.004),vulnerable plaque (P =0.01) and poor compliance of secondary prevention medication (P =0.02) were independent risk factors for recurrent ischemic stroke with initial brainstem infarction.Health education should be strengthened for preventing recurrent ischemic stroke with brainstem infarction.

Objective To analyze the abortion mental state investigation and related factors of negative emotions in unin tended pregnancy. Methods A total of 343 cases of unintended pregnancy by abortion (study group) and 100 patients had normal pregnancy pregnant maternity needs (control group) for the study, used the SCL-90 scale and DSQ scale evaluation of two emotional states and psychological defense way. Results There were significant differences of anxiety, depression, phobia, hostility and interpersonal sensitivity factor scores showed between two groups(P<0.05). There were significant differences of 4 psychological defense mechanism between two groups (P<0.05), the study group was more inclined to immature defense style , middle defense mode and hide. Unmarried, immature defense style , hide non-pregnant women, unwanted pregnancy abortion independent risk factors for negative emotions (P<0.05), education level, knowledge of the extent of fertility awareness were protective factor (P<0.05). Conclusion Unwanted pregnancy by abortion obvious bad mood , low educational level, lack of knowledge about fertility, premarital sex, negative psy-chological defense style and unintended adverse pregnancy outcomes and emotions.

Objective To explore the influence of different treatment time on the relapse and prognosis of patients with newly diagnosed epilepsy. Methods To prospectively studied the clinical characteristics of 155 patients with newly diagnosed epilepsy. Patients were separated into immediate ( n =84, seizures ≤3 times) or deferred (n =71, seizures ＞ 3 times) treatment groups according to number of seizures before treatment with appropriate antiepileptic drugs (AEDs). The patients were followed up for at least one year (median, 29 months). Kaplan-Meier survival statistics was used to analyze time to first seizure or time to treatment failure (inadequate seizure control and (or) intolerable side-effects ). The proportions of patients with treatment failure and seizure free during follow up were also compared. Results There was no significant difference in time to first seizure or time to treatment failure between immediate ( 1484 days and 2992 days) and deferred treatment ( 1104 days and 1964 days; Log-Rank test x2 =0. 571 and 0. 018 respectively, P = 0. 450 and 0. 893 ). Subgroup analyses according to etiology ( primary and cryptogenic/symptomatic epilepsy) and age (children ≤ 16 years; adult ＞ 16 years) did not reveal any difference between immediate and deferred treatment. During follow up, there were 20 treatment failure patients ( 23. 8% ) in immediate treatment group and 16 ( 22.5% ) in deferred treatment group ( no statistical difference, x2 =0. 035 ,P =0. 852). There were 40 seizure free patients (47. 6% ) in immediate treatment group and 30 (42. 3% ) in deferred treatment group ( no statistical difference, x2 = 0. 447, P =0. 504 ). Conclusions For newly diagnosed epilepsy patients with a few seizures ( seizures ≤ 3 ), immediate AEDs treatment does not affect the relapse and prognosis.

Objective To improve the diagnosis level of solitary bone plasmacytoma (SBP) through analysing the clinical characteristics and therapy of this disease. Methods The clinical data of 18 cases were retrospectively analysis since 1999 in five hospitals, the clinical characteristics and therapeutic effect was summarized. Results The average age of 18 cases is 54.2 year (35-78), male were 1.6 times than female. 8 of 10 patients survived for 1-10 year after chemoradiotherapy, 2 of them progressed to multiple myeloma (MM)and died. 5 of 8 patients survived after only chemotherapy or radiotherapy. There were 11 cases of all patients in continue complete remission (CCS), the average CCS time was 47.3 months. Conclusion SBP is a type low-grade malignant tumor. Chemotherapy and radiotherapy is the main therapy. The patients can gain satisfactory prognosis with chemoradiotherapy, part of them can progress to MM.

Objective To summarize the experience in hepatic artery reconstruction in adult-to-adult living donor liver transplantation(ALDLT).Methods Fifty patients underwent ALDLT in our hospital from January 2002 to July 2006.All the hepatic a~ery reconstructions were done under surgical microscope.ResultsTwo patients(4%)presented with hepatic artery thrombosis.All the patients were followed up for 2 to 52 months (median,9 months),and no hepatic artery stenosis nor hepatic artery pseudoaneurysm occurred.The 1-year survival rate was 92%(46/50).Conclusions Systematic evaluation of hepatic artery reconstruction and use of microsurgical technique are key to the reduction of complications of hepatic artery reconstruction in ALDLT.

Objective To explore the indications for liver transplantation among patients with hepatolithiasis.Methods Data from 1 431 consecutive patients with hepatolithiasis who underwent surgical treatment from January 2000 to December 2006 were retrospectively collected for analysis.Surgical procedures included T-tube insertion combined with intraoperative cholangioscopic removal of intrahepatic stones,hepatectomy,cholangiojejunostomy,and liver transplantation.Results Nine hundred and sixty-one patients who had a stone located in the left or right intrahepatic duct underwent hepatectomy or T-tube insertion combined with intraoperative cholangioscopic removal of intrahepatic stones.The rate of residual stones was 7.5%(72/961).Four hundred and seventy patients who had a stone located in the bilateral intrahepatic ducts underwent surgical procedures other than liver transplantation;the rate of residual stones was 21.7%(102/470).Only 15 patients with hepatolithiasis underwent liver transplantation;they all survived.According to the degree of biliary cirrhosis,recipients were divided into 2 groups: a group with biliary decompensated cirrhosis(n=7),or group with biliary compensated cirrhosis or noncirrhosis group(n=8).There were significant differences in operative times,transfusion volumes and blood losses between 2 groups(P