Objective To systematically evaluate and sequentially analyze the clinical efficacy and safety of Baogong Zhixue Granules for the treatment of abnormal uterine bleeding due to ovulation disorder.Methods The randomized controlled trials(RCTs)of Baogong Zhixue Granules for the treatment of abnormal uterine bleeding due to ovulation disorder were retrieved from the databases of CNKI,Wanfang Data,VIP,China Biology Medicine,PubMed,Embase,and Cochrane Library from the establishment of the databases to January 5th,2023.Literature screening and data extraction were conducted according to Cochrane Handbook for Systematic Reviews of Interventions,and Meta-analysis was performed by RevMan 5.4.Results Totally 15 articles were included,involving 1 425 patients.Meta-analysis results showed that Baogong Zhixue Granules could effectively improve the clinical efficacy of abnormal uterine bleeding due to ovulatory disorder(RR=1.17,95%CI[1.13,1.23],P<0.000 01),shorten hemostasis time[bleeding control time(MD=-6.35,95%CI[-8.89,-3.81],P<0.000 01),complete hemostasis time(MD=-12.56,95%CI[-16.22,-8.89],P<0.000 01)],reduce endometrial thickness(MD=-1.26,95%CI[-1.71,-0.80],P<0.000 01),reduce hemoglobin loss(MD=13.35,95%CI[11.41,15.29],P<0.000 01),improve the score of uterine bleeding(MD=-2.22,95%CI[-2.43,-2.01],P<0.000 01),and reduce the recurrence of disease(RR=0.37,95%CI[0.19,0.72],P=0.004).There was no statistical significance between the test group and the control group in terms of adverse reactions(P=0.82).The trial sequential analysis of the total efficiency was carried out,and the cumulative included research passed the traditional threshold and trail sequential analysis threshold.Conclusion Baogong Zhixue Granules have a certain clinical efficacy in the treatment of abnormal uterine bleeding due to ovulation disorder,but there may be publication bias in the study,and higher quality clinical RCTs need to be carried out.

Objective:To establish a HPLC characteristic spectrum method of Centipeda minima based on standard decoction; To evaluate the correlation of materia medica, decoction pieces, standard decoction, intermediates and formula granule; To provide a reference for the quality standard research of Centipeda minima formula granules.Methods:The materia medica, decoction pieces, standard decoction, intermediates and formula granules of Centipeda minima were analyzed by a HPLC characteristic spectrum method, and the software "Similarity Evaluation System for Chromatographic Fingerprint of TCM" was employed to conform the common peaks. The clustering analysis (CA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were adopted to find out the correlation of materia medica, decoction pieces, standard decoction, intermediates and formula granules of Centipeda minima. The Iconic components which can make differences was screened at the same time.Results:The characteristic chromatogram of materia medica, decoction pieces, standard decoction, intermediates and formula granules of Centipeda minima shared high similarity with the respective control chromatogram. Fourteen common peaks were confirmed, and eleven chromatographic peaks were identified. The results of CA and PCA were consistent, and the standard decoction, intermediates and formula granules of Centipeda minima were closer. Six differential components with VIP values greater than 1 were screened by OPLS-DA.Conclusions:The method is stable, accurate and characteristic. The study can provide a reference for the quality standard research of Centipeda minima Formula Granules by analyzing the correlation of characteristic spectrum of materia medica, decoction pieces, standard decoction, intermediates and formula granules.

Objective:To explore the teaching reform effect of the integrated "theory virtual reality" course in Fundamentals of Nursing in vocational colleges, providing reference for related research in other courses. Methods:From January to June 2023, a convenience sampling method was used to select vocational nursing students from 11 classes of the Nursing College of Guangxi Health Vocational and Technical College in 2022 as the research objects. Through class experimental research methods, a smart simulation virtual classroom was used to carry out the integrated teaching of Fundamentals of Nursing (referred to as "theory virtual reality") in theory virtual practice. The teaching resources and pre class, in class, and post class tasks were published on the Chaoxing Learning Platform. Theory practical teaching was conducted in the simulated virtual smart classroom, and virtual training scenes were constructed using virtual simulation software. Operations evaluation, planning, implementation, and evaluation were completed by forming groups and using training related molds. In class, live broadcasting was used to display the details of student operations, and Chaoxing Learning Platform was used for teaching. The platform conducts interactive activities such as group discussions, answering questions, and testing to achieve integrated teaching of "theory virtual reality". Before and after intervention, the Chinese version of the Critical Thinking Ability Scale and Nurse Competency Scale were selected to evaluate the critical thinking ability and nursing competency of nursing students. The Simulated Teaching Design Scale (SDS) was used to evaluate student satisfaction with teaching. Results:A total of 650 nursing students were included in this study, including 89 males and 561 females, aged (18.00 ± 1.20) years old. The total score of critical thinking before intervention was (247.17 ± 33.63) points, and compared with the score after intervention (294.16 ± 33.63) points, the difference was statistically significant ( t=-10 182.33, P<0.05). The total score of nursing competence before intervention was (485.09 ± 147.68) points, and compared with the score after intervention (507.09 ± 147.68) points, the difference was statistically significant ( t=-7 154.72, P<0.05) points. The approval rate of SDS was over 87%, and the total approval rate of the five dimensions was over 95%. Conclusions:The teaching reform of the Fundamentals of Nursing course in vocational colleges based on the integration of "theory virtual reality" can help improve students′ experience and interactivity, enhance their critical thinking and nursing competence, and achieve high satisfaction. It is worth promoting.

The Omicron family of SARS-CoV-2 variants are currently driving the COVID-19 pandemic. Here we analyzed the clinical laboratory test results of 9911 Omicron BA.2.2 sublineages-infected symptomatic patients without earlier infection histories during a SARS-CoV-2 outbreak in Shanghai in spring 2022. Compared to an earlier patient cohort infected by SARS-CoV-2 prototype strains in 2020, BA.2.2 infection led to distinct fluctuations of pathophysiological markers in the peripheral blood. In particular, severe/critical cases of COVID-19 post BA.2.2 infection were associated with less pro-inflammatory macrophage activation and stronger interferon alpha response in the bronchoalveolar microenvironment. Importantly, the abnormal biomarkers were significantly subdued in individuals who had been immunized by 2 or 3 doses of SARS-CoV-2 prototype-inactivated vaccines, supporting the estimation of an overall 96.02% of protection rate against severe/critical disease in the 4854 cases in our BA.2.2 patient cohort with traceable vaccination records. Furthermore, even though age was a critical risk factor of the severity of COVID-19 post BA.2.2 infection, vaccination-elicited protection against severe/critical COVID-19 reached 90.15% in patients aged ≽ 60 years old. Together, our study delineates the pathophysiological features of Omicron BA.2.2 sublineages and demonstrates significant protection conferred by prior prototype-based inactivated vaccines.
Humans ; Aged ; Middle Aged ; COVID-19/prevention & control* ; SARS-CoV-2 ; Pandemics/prevention & control* ; China/epidemiology* ; Disease Outbreaks/prevention & control* ; Vaccination

Objective:To explore a fast method to identify and confirm suspected clonorchis sinensis infection.Methods:For suspected clonorchis sinensis infection, the clonorchiasis serum antibody was detected first with ELISA. If the antibody was positive, the fecal examination for eggs was performed. If the fecal examination was negative, duodenal drainage under gastroscopy was recommended to detect eggs from the drainage fluid.Results:A total of 126 patients met the requirements and aged 54.14±13.33 (24- 87). There were 83 cases (65.87%, 83/126) with eggs positive in the drainage fluid, of which 53 cases were male, aged 55.91±11.47 (30-86), and 30 cases female, aged 55.87± 13.85(30-87). There was no significant difference in age between males and females( P>0.05). The time of catheterization (T1) of 126 cases was 3.79 ±1.45 min. The time of drainage (T2) of 126 cases was 31.39 ±14.29 min. There was no significant difference in T1 or T2 between the positive group and the negative group( P>0.05). The detection rates of eggs were 91.57% (76 cases) in intrahepatic bile duct drainage, 81.93% (68 cases) in the bile-cyst juice and 75.90% (63 cases) in the common bile duct fluid. No serious adverse reactions occurred during or after the operation. Conclusion:The detection rate of clonorchiosis sinensis can be effectively improved by the combination of clonorchiasis serum antibody test and gastroscopy-guided duodenal drainage.

Objective: To explore the current situation of college students’ delivery food consumption and the related factors, and to provide reference for healthy eating behaviors among college students. Methods: A random cluster sampling was used to select 2 597 student sample of various majors and grades of Wuhan University of Science and Technology. The self-made questionnaire was used to investigate the frequency and causes of college students ordering delivery food, as well as the status of nutrition knowledge, attitude and behavior. The Zung Depression Self-rating Scale (SDS) was adopted to measure the mental health status of the students, and height and weight were measured by a unified height and weight scale. Finally, the influencing factors mentioned above and consumption frequency of delivery food was analyzed. Results: Among the college students,60.0% reported the habit of ordering delivery food; As for the frequency of delivery food consumption, boys were singnificantly higher than girls(OR=1.38), students of first(OR=0.48) and third grade(OR=0.61) were lower than that of fourth grade,the medicine major’s frequency was lower than the science and engineering major’s(OR=0.57), and the art major’s was higher than the science and engineering’s(OR=1.94); The frequency of students who had received relevant health education was lower than the unreceived students’(OR=0.70); Students who had better performance in the nutrition learning attitude, and those who had healthier eatting habits (OR=0.62, 0.49); Students who saving reckoned delivery food as convenient, time and tasty (OR=4.77, 4.82, 2.85); Howver, students’ BMI, the score of depression scale, nutritional knowledge score, knowledge and practical attitude,diet structure score, food packaging, price and nutritional factors showed no significant effect on the frequency of delivery food consumption (P>0.05). Conclusion It is common for college students to order delivery food. gender, grade, major, nutrition and health education background, nutritional attitude, eating habits and convenient, time-saving, tasty nature of fast food are the factors influencing the frequency of delivery food consumption.

OBJECTIVE： To establish a method for simultaneous determination of α-pinene， β-pinene， limonene and α-terpineol in volatile oil of Forsythia suspensa. METHODS： GC method was adopted. The determination was performed on HP-5 capillary column through temperature-programmed route. The inlet temperature was 230 ℃， and detector temperature was 250 ℃； split sampling was applied （split ratio of 8 ∶ 1）； the air flow rate was 300 mL/min， the hydrogen flow rate was 30   mL/min， the tail gas flow rate was 30 mL/min， and the injection volume was 1 μL. Using limonene as internal reference， relative correction factors of α-pinene， β-pinene and α-terpineol were established， and the reproducibility of relative correction factors were investigated by using different chromatographs and columns， and chromatographic peak location of components was measured. The contents of above components were calculated with QAMS， and then compared with the results of external standard method. RESULTS： The linear range of α-pinene， β-pinene， limonene and α-terpineol were 16.5-990.0， 38.1-2 287.5， 8.2-491.2， 2.4-142.5  μg/mL， respectively （r≥0.999 1）. RSDs of precision， reproducibility and stability tests were all lower than 3％ （n＝6）. Average recoveries were 99.7％-105.5％（RSD＜4％，n＝9）. Compared with limonene （1.00），the average relative correction factors of α-pinene， β-pinene and α-terpineol were 0.91，0.86 and 1.11（n＝3）； relative retention time were 0.69-0.74， 0.81-0.86， 1.25-1.35（RSD＜3％，n＝3）. By using different chromatographs and columns， RSDs of relative correction factors were 0.21％-4.65％（n＝6）. Compared with external standard method， determination results of above 4 components were consistent （the absolute value of relative error were all less than 7％）. CONCLUSIONS： QAMS can be used for simultaneous determination of 4 kinds of effective components in volatile oil from F. suspensa.

Objective To investigate the risk factors for malignant middle cerebral artery infarction (MMI) in different age groups.Methods Patients with middle cerebral artery infarction were analyzed retrospectively.They were divided into a young and middle-aged group (＜ 60 years) and an elderly group (≥60 years).The demography and vascular risk factors,stroke etiologies,baseline blood pressure,drug use before onset and common blood test results in patients with MMI and non-MMI were compared in general cases and each age group.Multivariate logistic regression analysis was used to determine the independent risk factors for MMI.ResultsA total of 912 patients with middle cerebral artery infarction were enrolled,including 299 females (32.79％) and 613 males (67.21％);401 young and middle-aged patients (43.97％) and 511 elderly patients (56.03％);159 patients in the MMI group (17.43％),and 753 (82.57％) in the non-MMI group.Multivariable logistic regression analysis showed that hypertension (odds ratio [OR]6.962,95％ confidence interval [CI] 1.349-35.934;P=0.021) and NIHSS score (OR 1.551,95％ CI 1.384-1.737;P ＜0.001) were the independent risk factors for MMI.Subgroup analysis showed that NIHSS score (OR 1.402,95％ CI 1.239-1.588;P＜0.001) was an independent risk factor for MMI in young and middle-aged patients;hypertension (OR 10.752,95％ CI 1.213-95.295;P =0.033),diastolic blood pressure (OR 1.080,95％ CI 1.002-1.164;P=0.044),and NIHSS score (OR 1.504,95％ CI 1.281-1.765;P＜ 0.001) were the independent risk factor for MMI in the elderly patients,while systolic blood pressure (OR 0.938,95％ CI 0.893-0.986;P =0.011) was an independent protective factor.Conclusions The baseline NIHSS score was an independent risk factor for MMI in each age group.Hypertension and diastolic blood pressure were the independent risk factors for MMI in the elderly patients,while systolic pressure was an independent protective factor.Therefore,timely NIHSS assessment and appropriate hypertension management had important significance for the prevention and treatment of MMI.

Animals ; Antibodies, Monoclonal ; immunology ; Cell Line ; Cercopithecus aethiops ; Cricetinae ; Culicidae ; Dengue Virus ; immunology ; Encephalitis Virus, Japanese ; immunology ; Encephalitis, Japanese ; immunology ; Mice ; Mice, Inbred Strains ; Vero Cells ; Viral Vaccines ; immunology

Objective Based on the theory of Service Quality to develop a scale for measuring personal digital assistant satisfaction by nurses and to detect the reliability and validity of this scale. Methods Through the literature research, cross-sectional study and present satisfaction evaluation tool for using personal digital assistant by nurse to build the item pool. The items were selected by 15 experts consultation and the pilot survey of 666 nurses. Results The satisfaction evaluation tool for using personal digital assistant by nurse scale consisted of 41 items;the exploratory factor analysis identified 8 principal factors and explained for 65.22%. Pearson correlation coefficient between each dimension was 0.213-0.684(P<0.01). Pearson correlation coefficient between each dimension and total scale was 0.574-0.798(P<0.01). The Cronbach α coefficient of the scale was 0.928 and test-retest reliability was 0.934. Conclusions The satisfaction evaluation tool for using personal digital assistant by nurse scale has good validity and reliability. It can be used as a tool to measure the satisfaction for using personal digital assistant by nurse.