Objective:To investigate the effects of bracket-free invisible orthodontic technique versus traditional fixed orthodontic technique on the levels of inflammatory factors in the gingival crevicular fluid of orthodontic adolescents. Methods:A total of 67 orthodontic patients who received their first orthodontic treatment in Huzhou Central Hospital from June to September 2020 were included in this study. They were randomly divided into an observation group ( n = 34) and a control group ( n = 33). The control group was treated with traditional fixed orthodontic treatment. The observation group was treated with invisible orthodontic treatment without brackets. At 0, 1, 2, 4, and 6 months of treatment, the level of inflammatory factors and periodontal index were compared. Results:At 0 and 1 month of treatment, there were no significant differences in the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in the gingival crevicular fluid between the observation and control groups (all P > 0.05). At 2, 4, and 6 months of treatment, the levels of IL-1β, IL-6, and TNF-α in the observation group were significantly lower than those in the control group (IL-1β: t2 months = 5.56, P = 0.042; t4 months = 8.14, P = 0.019; t6 months = 9.87, P = 0.002. IL-6: t2 months = 7.15, P = 0.029; t4 months = 7.94, P = 0.021; t6 months = 9.16, P = 0.007. TNF-α: t2 months = 6.87, P = 0.039; t4 months = 7.65, P = 0.026: t6 months = 9.89, P = 0.001). In each group, gingival index (GI), sulcus bleeding index (SBI), probing depth (PD), and plaque index (PLI) increased significantly after treatment. At 0 and 1 month of treatment, there were no significant differences in GI, SBI, PD, and PLI between the two groups (all P > 0.05). At 2, 4, and 6 months of treatment, GI, SBI, PD, and PLI in the observation group were significantly lower than those in the control group (GI: t2 months = 3.62, P = 0.073; t4 months = 8.16, P = 0.018; t6 months = 8.54, P = 0.016. SBI: t2 months = 5.65, P = 0.042; t4 months = 7.56, P = 0.027; t6 months = 8.15, P = 0.019. PD: t2 months = 5.652, P = 0.042; t4 months = 7.56, P = 0.027, t6 months = 8.15, P = 0.019. PLI: t2 months = 9.57, P = 0.006; t4 months = 9.98, P = 0.002. t6 months = 9.94, P = 0.010). In addition, at 1 month of treatment, PLI in the observation group was significantly lower than that in the control group ( t = 9.99, P = 0.001). Conclusion:Bracket-free invisible orthodontic treatment provides better protection on periodontal tissue and has less impact on periodontal health than traditional fixed orthodontic treatment.

Objective:To investigate the expression of miR-146a and miR-155 in gingival crevicular fluid (GCF) of patients with peri-implantitis and their relationships with bone resorption.Methods:80 implants of 80 patients with peri-implantitis from October 2015 to December 2018 were selected as the disease group and 80 implants of 80 peri-implant healthy persons checked at the same time were selected as the control group. The GCF of the examined teeth was collected with moisture absorbent paper tips; the expression levels of miR-146a and miR-155 in GCF were detected by quantitative real-time polymerase chain reaction (qRT-PCR); the alveolar bone absorption around the implant was measured by X-ray; the levels of inflammatory factors such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), osteopontin (OPN) in GCF were detected by enzyme-linked immunosorbent assay (ELISA); Pearson method was used to analyze the expression levels of miR-146a and miR-155 in GCF and their correlations with bone resorption, IL-1β, TNF-α and OPN levels.Results:Compared with control group, probe depth (PD) (4.56±0.73)mm, plaque index (PLI) (2.21±0.44), sulcus bleeding index (SBI) (2.69±0.32), bone resorption (1.86±0.34), and miR-146a (2.46±0.43), miR-155 (4.20±0.57), IL-1β (75.48±10.29)ng/ml, TNF-α (54.16±9.23)ng/ml and OPN levels (252.41±42.03)pg/L in GCF were significantly higher than those in the disease group [(1.47±0.26)mm, (1.02±0.19), (0.94±0.21), (0.47±0.09), (0.97±0.16), (1.03±0.21), (44.52±8.14)ng/ml, (21.45±4.27)ng/ml, (181.91±36.28)pg/L] (all P<0.05). Pearson analysis showed that the levels of miR-146a and miR-155 in GCF of patients with peri-implantitis were positively correlated ( r=0.558, P<0.05), and they were correlated with bone resorption, IL-1β, TNF-α and OPN levels respectively ( r=0.554, 0.606, 0.521, 0.499; 0.515, 0.653, 0.585, 0.542; all P<0.05). Conclusions:MiR-146a and miR-155 are highly expressed in gingival crevicular fluid, which are positively correlated, and may affect bone resorption in patients with periimplantitis through inflammatory reaction.

Objective To investigate the photoregulation of transient receptor potential ankyrin 1 (TRPA1) in HaCaT cells,and to explore its mechanisms.Methods Cultured HaCaT cells were divided into 225-mJ/cm2 UVA radiation groups and 25-mJ/cm2 UVB radiation groups.HaCaT cells in the UVA radiation groups were further classified into 6 groups:blank control group 1 receiving no treatment,retinal group 1 treated with 12 μmol/L retinal alone,UVA group treated with 225 mJ/cm2 UVA radiation alone,retinal + UVA group (UVA-TRPA1 control group),retinal + UVA + 500 μmol/L cinnamaldehyde group (UVA-TRPA1 agonist group) and retinal + UVA + 1 mmol/L camphor group (UVA-TRPA1 antagonist group).Additionally,HaCaT cells in the UVB radiation groups were also further classified into 6 groups:blank control group 2 receiving no treatment,retinal group 2 treated with 12 μmol/L retinal alone,UVB group treated with 25-mJ/cm2 UVB radiation,retinal + UVB group (UVB-TRPA1 control group),retinal + UVB + 500 μmol/L cinnamaldehyde group (UVB-TRPA1 agonist group) and retinal + UVB + 1 mmol/L camphor group (UVB-TRPA1 antagonist group).Real-time fluorescence-based quantitative PCR (qPCR) and Western blot analysis were performed to determine the mRNA and protein expression of TRPA 1 respectively.Flow cytometry was conducted to investigate changes of calcium influx in HaCaT cells in the above groups.Results qPCR and Western blot analysis showed that TRPA1 mRNA and protein were expressed in HaCaT cells.The fluorescence intensity of calcium influx significantly differed among the blank control group 1,retinal group 1,UVA group and retinal + UVA group (155.06 ± 7.62,148.37 ± 18.77,166.92 ± 3.71 and 331.333 ± 40.563;F =44.509,P ＜ 0.01),as well as among the blank control group 2,retinal group 2,UVB group and retinal + UVB group (150.20 ± 1.73,171.66 ± 56.23,147.56 ± 6.60 and 250.44 ± 9.13;F =85.261,P ＜ 0.01).Additionally,retinal + UVA/UVB groups showed significantly higher fluorescence intensity of calcium influx compared with the blank control groups (q =18.442,6.052,P ＜ 0.01).The TRPA1 agonist cinnamaldehyde and its antagonist camphor could regulate the UVA-and UVB-induced calcium influx (P ＜ 0.001).Compared with the blank control group 1 and 2 respectively,the fluorescence intensity of retinal-dependent calcium influx was significantly higher in the UVA/UVB-TRPA1 agonist group (q =14.934,32.770,P ＜ 0.001),and significantly lower in the UVA/UVB-TRPA1 antagonist group (q =7.986,14.596,P ＜ 0.001).Conclusion TRPA1 is expressed in HaCaT cells,and UVA or UVB can regulate the calcium influx in HaCaT cells by adjusting the activity of TRPA 1.

OBJECTIVE: To evaluate the efficacy of intratympanic dexamethasone injection for the moderate and severe sudden deafness with BPPV. METHOD: A total of 63 patients diagnosed with sudden sensorineural hearing loss with BPPV were treated through OPD. Patients were divided into three groups: 20 cases in intratympanic dexamethasone injection as initial treatment (group A); 18 cases in systemic hormone therapy group (group B); 25 cases in intratympanic dexamethasone injection as salvage treatment (group C). In addition, routine drugs were used to all patients. RESULT: The overall effective rate of group A, B and C in hearing recovery was 60.0%, 38.9% and 48.0%, respectively: (1) No significant difference of hearing recovery was observed among three groups (P > 0.05); (2) A significant difference of hearing recovery was evidenced between group A and C (P < 0.05); (3) A significant difference of hearing recovery was evidenced between group A and C (P < 0.05); (4) No statistically significant difference was found in the hearing recovery between group B and C (P > 0.05). CONCLUSION Our data showed that intratympanic dexamethasone should be used as initial therapy for treating the moderate and severe sudden deafness with BPPV.
Benign Paroxysmal Positional Vertigo ; complications ; Dexamethasone ; administration & dosage ; therapeutic use ; Hearing Loss, Sensorineural ; complications ; drug therapy ; Hearing Loss, Sudden ; complications ; drug therapy ; Hearing Tests ; Humans ; Injection, Intratympanic ; Salvage Therapy ; Treatment Outcome

OBJECTIVE: To investigate the prognosis of sudden deafness patients with benign paroxysmal positional vertigo (BPPV). METHOD: The clinical data of 24 sudden deafness patients with BPPV was analyzed. The outcome of 125 sudden deafness patients without BPPV at the same time was compared. RESULT: Hearing improvement after three months treatment was 41.67% and 72.80% in sudden deafness patients with BPPV and sudden deafness patients without BPPV, respectively. The difference was statistically significant (P<0.05). CONCLUSION The prognosis of hearing in sudden deafness patients with BPPV is worse than that in sudden deafness patients without BPPV. BPPV may predict a poor hearing outcome in sudden deafness.
Adolescent ; Adult ; Aged ; Benign Paroxysmal Positional Vertigo ; complications ; diagnosis ; Female ; Hearing Loss, Sudden ; complications ; diagnosis ; Humans ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Young Adult

Objective To analyze the laboratory index characteristics of pulmonary hypertention (PH ) patients with different function states and pressure stages .Methods 2 752 patients diagnosed with PH in the outpatient department ,emergency depart-ment and inpatient department of this hospital from January 2006 to December 2011 were retrospectively analyzed .The clinical as-sessment of the disease condition was done according to the function state and pressure stage of PH recommended by WHO .The characteristics of hematological indexes ,inflammatory indicators and blood gas analysis were observed as well .Results The most common hematological abnormal indexes were the serum brain natriuretic peptide (BNP) elevation(89 .12% ) ,abnormal liver func-tion(58 .32% ) and abnormal hemoglobin(58 .32% ) .The proportion of the PH patients with the elevation of erythrocyte sedimenta-tion rate(ESR) and high-sensitivity C-reactive protein (hs-CRP) were 78 .52% and 73 .59% respectively .The constituent ratio of the BNP increase ,liver function abnormality ,abnormal hemoglobin ,elevation of UA and ESR had statistical differences among the PH patients with different function states and pressure stages (P<0 .05) .The most commonly blood gas analysis abnormality was hypoxemia(83 .08% ) .Respiratory alkalosis had the highest incidence rate in the acid-base imbalance(24 .58% ) .Conclusion The most common hematological abnormal indexes among PH patients are the elevation of serum BNP ,abnormal liver function and he-moglobin abnormality .The laboratory abnormal indexes of above 3 items and the increase of UA and ESR are always related with the severity of disease ,which should to be followed-up .

Objective The analysis healthy crowd skull magnetic resonance image formation(MRI) inspection result,understood healthy crowd skull common disease the type,favors the healthy physical examination project the reasonable establishment.Methods Medical Center sent medical examination of 380 cases of brain MRI data and retrospective analysis.Results 380 cases of brain MRI results show healthy physical examination:there are 9 kinds of disease,MRI exception occurred there were 109 cases,abnormal rate of 28.7％ ; by age is divided into three groups:30-40 sui,41-50 sui; 51-60 sui; 51-60 sui exception rate was highest in the age group,to 56.9％ ; the discovery of the disease,the highest incidence of disease with multiple lacunar cerebral infarction,73.4％ per cent of total incidence.Conclusion MRI no radiation,brain MRI examination should as a general project of middle and old aged healthy,early detection of brain tumor lesions and cerebral vascular lesion,disease prevention and healthy living for guidance.

To explore the diagnostic value of susceptibility weighted imaging (SWI) for intracranial cavernous hemangioma.The magnetic resonance sequences of SWI,conventional imaging (MRI) and diffusion-weighted imaging (DWI) were performed in 37 patients of intracranial cavernous hemangioma to compare their different imaging capacities.Among them,the number of lesions found on T1WI,T2WI,DWI and SWI sequences were 40,41,42 and 50 respectively.Thus SWI may detect regular MRI negative or smaller lesions of intracranial cavernous hemangioma.

Objective To investigate the roles of glutamate signaling pathway in the activation of human peripheral blood lymphocytes(PBLs) and pathogenesis of vitiligo.Methods Peripheral blood lymphocytes (PBLs) isolated from 5 patients with generalized vitiligo and 5 healthy controls were cultured in vitro.Flow cytometry was performed to quantify the expression of CD25 and interferon-γ on PBLs derived from healthy controls and treated with MK801 (a non-competitive antagonist of N-methyl-D-aspartic acid receptor,NMDAR) at 100 μmol/L or phosphate buffered saline (PBS) for 48 hours,as well as the level of reactive oxygen species (ROS) in the controlderived PBLs treated with MK801 at 100 μmol/L,NMDA (an agonist of N-methyl-D-aspartic acid receptor) at 0.5 mmol/L or PBS for 48 hours.The protein and mRNA expressions of NMDAR1 and NMDAR2A were measured by flow cytometry and real-time PCR,respectively,in PBLs from the healthy controls and vitiligo patients.Immunohistochemistry was used to observe the expressions of NMDAR1 and NMDAR2A in tissue specimens from depigmented and postinflammatory hyperpigmentation lesions of the patients with vitiligo and from normal skin of the healthy controls.Results Compared with the PBS-treated PBLs from the healthy controls,the MK801-treated PBLs showed a downregulated expression of CD25 (7.28％ ± 0.18％ vs.16.02％ ± 0.42％,P ＜ 0.01),but an upregulated proportion of CD25+IFN-γ+ lymphocytes (1.79％ ± 0.09％ vs.0.78％ ± 0.06％,P ＜ 0.01),and the NMDA-treated PBLs displayed a higher ROS level (101.1 ± 3.50 vs.69.80 ± 2.08,P＜ 0.01 ).The protein expression of NMDAR1 in PBLs was significantly higher in vitiligo patients than in the healthy controls (3.85 ± 2.17 vs.0.97 ±0.55,P ＜ 0.05).Conclusion Glutamate signaling pathway may be involved in the immunopathogenesis of vitiligo via affecting the secretion of interferon-γ by,and ROS level in,activated lymphocytes.

Objective To investigate the diagnosis value of immunophenotype and karyotypes in newly diagnosed chronic lymphocytic leukemia (CLL).Methods To retrospect the flow cytometry (FCM) immunophenotype and karyotypes characteristics in newly diagnosed 70 CLL cases.Results In all cases,the positive rates of CD19,CD20,CD5,CD23,CD22 were 100 ％,88.5 ％ (54/61),77.1％ (54/70),67.6 ％ (23/34)and 51.9 ％ (27/52),respectively.And 6 were misdiagnosed,2 was CD+5CD+19(+),but CD20,CD22 were strongly positive,final diagnosed as mantle cell lymphoma (MCL) by FISH t(11;14) examination and CyclinDl; CD+5CD+19(-) CLL were 16 cases (22.9 ％),but 4 were misdiagnosed,the misdiagnosis rate was 25 ％,significantly higher than that of CD+5CD+19(-) CLL (P =0.030).59 cases were examined by conventional cytogcnetic (CC),and 13 cases were with abnormal karyotypes,positive rate was 22.0 ％,with complex karyotypes in 5 cases (8.5 ％); 10 cases combined with FISH abnormalities karyotype examination rate was 60 ％.Conclusion Typical CLL immunophenotypic characteristics were CD5,CD19,CD23 co-expression,and CD－5 CLL with higher misdiagnosis rate,combined with CD20 (,) CD22 expression and karyotype analysis helps to CLL and other B lymphoid proliferative diseases (B-LPD) identification.Conventional cytogenetic detection combined with FISH scan can improve the recognition ability of abnormal chromosome.