Objective:To investigate the application value of ultrasound-guided selective pudendal nerve block during vestibular gland resection.Methods:Ninety patients who underwent vestibular gland resection in Lishui Maternal and Child Health Hospital from January 2017 to January 2020 were included in this study. These patients were divided into a control group and an observation group ( n = 45/group) according to the order of admission. Patients in the control group underwent blind nerve block and those in the observation group underwent ultrasound-guided selective pudendal nerve block. Hemodynamics before and after anesthesia, anesthetic effects, pain severity, and the incidence of complications were compared between the control and observation groups. Results:There were no significant differences in hemodynamics and pain severity between the control and observation groups (both P > 0.05). Systolic blood pressure [(124.37 ± 8.12) mmHg], diastolic blood pressure [(91.68 ± 5.44) mmHg] and heart rate [(74.62 ± 3.56) beats/min] were significantly lower than those in the control group [(128.62 ± 9.27) mmHg, (95.24 ± 6.17) mmHg, (76.12 ± 3.32) beats/min, t = 2.248, 2.903, 2.067, all P < 0.05]. In the observation group, pain scores at 2 and 24 hours after surgery were (3.06 ± 0.25) points and (4.21 ± 0.52) points, respectively, which were significantly lower than those in the control group [(3.28 ± 0.24) points and (4.54 ± 0.48) points, t = 2.618, 3.128, both P < 0.05). Anesthetic effect in the observation group was superior to that in the control group, and incidence of complications in the observation group was significantly lower than that in the control group ( χ2 = 12.869, 4.285, 4.864, 3.920, all P < 0.05). Conclusion:Ultrasound-guided selective pudendal nerve block in vestibular gland resection exhibits better anesthetic effect, has no obvious effect on hemodynamics, and has good analgesic effect with low incidence of complications. Therefore, it deserves clinical popularization.

Objective:To determine the dose-effect relationship of norepinephrine in the treatment of hypotension after spinal anesthesia in cesarean section.Methods:Ninety American Society of Anesthesiologists physical status Ⅰ or Ⅱ parturients who were at full term with a singleton fetus, body mass index of 20-35 kg/m 2, blood pressure 100-140 mmHg, heart rate 60-100 beats/min, scheduled for elective cesarean section, were divided into 6 different doses of norepinephrine groups (NE2, NE4, NE6, NE8, NE10 and NE12 groups) using a random number table method, with 15 cases in each group.The maternal basal systolic blood pressure was measured after entering the operating room.Anesthesia was performed by injecting hyperbaric bupivacaine 9 mg into the subarachnoid space over 45 s. When hypotension occurred for the first time after anesthesia (systolic blood pressure was lower than 80% of the baseline value), norepinephrine 2, 4, 6, 8, 10 and 12 μg (diluted to 5 ml in normal saline) were intravenously injected in NE2, NE4, NE6, NE8, NE10 and NE12 groups, respectively.Systolic blood pressure was measured at 60 s after completion of injection.The effective treatment of hypotension was defined as the recovery of systolic blood pressure to more than 80% of the baseline value.The logistic regression analysis method was used to draw the dose-effect curve of norepinephrine in treating hypotension after spinal anesthesia in cesarean section.The median effective dose (ED 50), 95% effective dose (ED 95) and 95% confidence interval (CI) were calculated.The time to first hypotension, effective treatment of hypotension, and occurrence of bradycardia and nausea and vomiting after intravenous injection of norepinephrine were recorded.The Apgar scores of the neonates at 1 and 5 min after birth were recorded.The umbilical artery blood samples of neonates were collected immediately after cutting the cord for blood gas analysis. Results:There was no significant difference in the incidence of maternal basal systolic blood pressure, time to first hypotension, bradycardia, and nausea and vomiting among the six groups ( P>0.05). The rate of effective treatment of hypotension increased with the increase of the dose in the six groups ( P<0.05). There was no significant difference in Apgar score and indexes of umbilical artery blood gas analysis at 1 and 5 min after birth among the six groups ( P>0.05). The ED 50 (95% CI) of norepinephrine in the treatment of hypotension after spinal anesthesia in cesarean section was 4.0 (3.0 to 5.0) μg, and the ED 95 (95% CI) was 11.8 (8.9-20.4) μg. Conclusion:The ED 50 and ED 95 of norepinephrine are 4.0 and 11.8 μg, respectively, when used for treating hypotension after spinal anesthesia in cesarean section.

Objective To analyze the influence of psychological interview combined with nostalgic guidance on mental health of patients with Alzheimer's disease.Methods A total of 50 patients with Alzheimer's disease in our hospital were divided into observation group and control group according to different nursing measures,with 25 cases in each group.The control group was given routine nursing care,while the observation group was given psychological interview combined with nostalgic guidance.The mental health level of two groups after intervention were compared.Results Observation group had lower Hamilton Depression scale (HAMD) scores,and higher Mini-mental State Examination (MMSE) scores than the control group (P ＜ 0.05).Conclusion The combination of psychological interview and nostalgic guidance is helpful to improve the depression of patients with Alzheimer's disease and improve their mental health.

Objective To analyze the influence of psychological interview combined with nostalgic guidance on mental health of patients with Alzheimer's disease.Methods A total of 50 patients with Alzheimer's disease in our hospital were divided into observation group and control group according to different nursing measures,with 25 cases in each group.The control group was given routine nursing care,while the observation group was given psychological interview combined with nostalgic guidance.The mental health level of two groups after intervention were compared.Results Observation group had lower Hamilton Depression scale (HAMD) scores,and higher Mini-mental State Examination (MMSE) scores than the control group (P ＜ 0.05).Conclusion The combination of psychological interview and nostalgic guidance is helpful to improve the depression of patients with Alzheimer's disease and improve their mental health.

Objective To study the sensitivity and specificity of the Chinese eating assessment tool (EAT-10) in screening acute stroke patients for oropharyngeal dysphagia (OD).Methods A total of 130 inpatients with acute stroke were screened using the Chinese EAT-10.On the same day they were also screened using the gold standard technique for diagnosing dysphasia-videofluoroscopy.A receiver operating characteristics (ROC) curve was developed to study EAT-10's sensitivity and specificity.A Youden index,positive predictive value (PPV),negative predictive value (NPV),and positive and negative likelihood ratios (LHR+ and LHR) were quantified.Results According to the ROC curve,a cut-off point of 1 (EAT-10 score≥ 1) gave the best sensitivity (77.9％),the highest NPV (73.2％),with 66.1％ specificity,71.6％ PPV,2.30 LHR+ and 0.33 LHR in screening for OD.The test-retest reliability was above 0.7.An investigator consistency reliability test showed good repeatability,and the consistency between each item and the mean total score was high.Conclusion The Chinese EAT-10 has good test-retest reliability and investigator consistency.The optimal cut-off point is 1,with good sensitivity and NPV at scores ≥ 1.The test can be recommended as a screening tool for OD in acute stroke patients.

Objective To investigate the expressions of metastasis suppressor 1 (MTSS1 )and calcium activated protein 43 (Cap43)in esophageal squamous cell carcinoma (ESCC)tissue,and to clarify the relationship between the expressions of MTSS1,Cap43 and the clinicopathological features of ESCC.Methods 80 cases of ESCC tissue and 30 cases of normal adjacent-cancer tissue were collected,and the protein and mRNA expressions of MTSS1 and Cap43 in ESCC tissue and normal tissue were detected by streptavidin-perosidase (SP)immunohistochemistry and RT-PCR;their relationships with the clinicopathological features of ESCC were analyzed.Results The positive expression rate of MTSS1 in normal esophagus tissue was significantly higher than that in ESCC tissue detected by SP (83.3%vs 21.3%,P<0.01)and RT-PCR (0.703±0.085 vs 0.295±0.065,P<0.01),However,the positive expression rate of Cap43 in normal esophagus tissue was significantly lower than that in ESCC tissue by SP method (16.7%vs 76.3%,P<0.01)and by RT-PCR (0.236±0.052 vs 0.693±0.078,P<0.01).The mRNA expression levels of MTSS1 and Cap43 in ESCC tissue were significantly related with the invasive extent, histological differentiation,TNM stage,and lymphatic metastases (P<0.05)of ESCC,but not related with the age,sex,tumor size and pathological type (P>0.05). The mRNA expression of MTSS1 was negatively correlated with the expression of Cap43 (r=-0.457,P<0.05).Conclusion The low-expression of MTSS1 and over-expression of Cap43 in ESCC tissue may contribute to tumor invasion and metastasis;the imbalance of MTSS1 and Cap43 may be one of the mechanisms of tumor invasion and metastases.

Purpose To discuss the feasibility of evaluating left ventricular systolic function in patients with acute anterior myocardial infarction (AAMI) with three-dimensional speckle tracking imaging (3D-STI). Materials and Methods Thirty AAMI patients were examined with 3D-STI technique in terms of left ventricular longitudinal strain (LS), radial strain (RS), circumferential strain (CS), area strain (AS), global longitudinal strain (GLS), global riadial strain (GRS), global circumferential strain (GCS) and global area strain (GAS). The results were compared with those of 30 healthy persons and the strain values in normal segments of those AAMI patients. The threshold, sensibility and specificity of those parameters in diagnosing myocardial infarction were analyzed. Results Compared with healthy group, LS reduced in 12 out of 17 segments (12/17), RS reduced in 10/17, CS in 9/17 and AS in 11/17;they mainly focused in the base segment, middle and apex of anterior wall, the base segment and the middle of anterior septal, the apex of aboral septal, and the middle segment, apex of the lateral wall. The GLS, GRS, GCS and GAS were all lower than those in healthy group (differences with statistic signiifcance:P<0.05). In AAMI patient, LS, RS, CS and AS in the infarcted segments were lower compared with those in the normal segments (differences with statistic signiifcance:P<0.05). ROC curve analysis showed that the sensitivity of LS, RS, CS and AS were 89.2%, 79.0%, 77.9%, 85.3%, respectively in the diagnosis of myocardial infarction, and the speciifcity were 65.8%, 71.0%, 66.9%and 92.3%, respectively. The sensitivity of the GLS, GRS, GCS and GAS were 91.2%, 74.0%, 68.9%, 85.3%, respectively in the diagnosis of myocardial infarction, and the speciifcity were 69.8%, 71.0%, 76.9%and 93.3%, respectively. Conclusion 3D-STI can detect the strain values of LV segments efficiently and accurately so as to discriminate normal and infracted segments, which provides a reliable measurement to evaluate the extent of the infarction of the patients with acute anterior wall myocardial infarction.

Obj ective To explore changes of plasma NT-proBNP and inflamatory cytokines levels before and after treatment for preeclampsia.Methods The levels of NT-proBNP(with fluore-scent immunoassay)and infammatory cytokines(IL-6 ,hs-CRP and TNF-αwith high-sensitive en-zyme immunoassay and radio immunoassay)in plasma were detected in 93 patients with preeclamp-sia (including 45 cases of light pre-eclampsia and 48 cases of severe pre-eclampsia),and 42 normal people were collected and detected for contrastive analysis.Results Before treatment,the plasma NT-proBNP and inflammatory cytokines (IL-6 ,hs-CRP and TNF-α)levels in 93 patients with preeclampsia were significantly higher than those in 42 normal people (P<0 .0 1 ).The plasma NT-proBNP,IL-6,hs-CRP and TNF-αlevels increased with increasing degree of preeclampsia.After treatment,the levels of NT-proBNP,IL-6,hs-CRP and TNF-αin 45 patients with light pre-eclampsia reached to normal level (P>0 .05 ).Though the plasma IL-6 level reached to normal lev-el,the levels of NT-proBNP,hs-CRP and TNF-αin 48 patients with severe pre-eclampsia were sig-nificantly higher than those in 42 normal people (P<0 .05 ).Conclusion The plasma NT-proBNP and inflammatory cytokines participate in pathogenesis process of preeclampsia,and these two indi-cators are effective monitoring index for the early treatment of preeclampsia.

Obj ective To explore changes of plasma NT-proBNP and inflamatory cytokines levels before and after treatment for preeclampsia.Methods The levels of NT-proBNP(with fluore-scent immunoassay)and infammatory cytokines(IL-6 ,hs-CRP and TNF-αwith high-sensitive en-zyme immunoassay and radio immunoassay)in plasma were detected in 93 patients with preeclamp-sia (including 45 cases of light pre-eclampsia and 48 cases of severe pre-eclampsia),and 42 normal people were collected and detected for contrastive analysis.Results Before treatment,the plasma NT-proBNP and inflammatory cytokines (IL-6 ,hs-CRP and TNF-α)levels in 93 patients with preeclampsia were significantly higher than those in 42 normal people (P<0 .0 1 ).The plasma NT-proBNP,IL-6,hs-CRP and TNF-αlevels increased with increasing degree of preeclampsia.After treatment,the levels of NT-proBNP,IL-6,hs-CRP and TNF-αin 45 patients with light pre-eclampsia reached to normal level (P>0 .05 ).Though the plasma IL-6 level reached to normal lev-el,the levels of NT-proBNP,hs-CRP and TNF-αin 48 patients with severe pre-eclampsia were sig-nificantly higher than those in 42 normal people (P<0 .05 ).Conclusion The plasma NT-proBNP and inflammatory cytokines participate in pathogenesis process of preeclampsia,and these two indi-cators are effective monitoring index for the early treatment of preeclampsia.

This article describes the development,components and function of the laboratory automation system (LAS) and laboratory information system(LIS). And it also discusses the construction and application laboratory automation,and the operation of the automated laboratory information system.