Objective To explore the relevant relationship and specificity between the implicit and explicit loyalty of military cadets in order to provide a theoretical basis and objective indicators for a more comprehensive and objective assessment for individual loyalty.Methods E-Prime 2.0,a classic implicit association paradigm was employed to construct an implicit association loyalty test for 64 military cadets.Simultaneously,an explicit loyalty measurement was conducted using the Chinese Military Personnel Loyalty Scale.Results ① Significant implicit effect was observed in the loyalty assessment of military cadets,indicating a general tendency to perceive higher levels of personal loyalty and lower levels of loyalty to external entities.② Explicit loyalty assessment revealed that the participants had the highest loyalty score towards the Party,the Nation,and the People(4.79±0.34),followed by the loyalty score to their profession(4.38±0.53),and the relatively lower loyalty score towards the unit and leaders(4.03±0.83).Among the 3 dimensions of loyalty,the normative loyalty score ranked highest,while continuance loyalty score took lower.③ There were no correlations among the scores of loyalty to the Party,the Nation,and the People(r=-0.030,P=0.823),to the profession(r=-0.047,P=0.728),to the unit(r=0.050,P=0.710),or to the leaders(r=0.043,P=0.749).Conclusion The implicit effect in the loyalty assessment is significant in military cadets,and there is no significant correlation between explicit and implicit loyalty measurements.Thus,we cannot rely solely on explicit measurements to assess their loyalty attitudes.

Remazolam is a new ultra-short-acting benzodiazepine that produces sedation by acting on γ-aminobutyric acid type A(GABAA)receptors.Remazolam has characteristics of rapid onset of action,short half-life,high clearance,and no accumulation in the body.It also has a low incidence of injection pain and mild effects on hepatic and renal function,respiration,and haemodynamics.Additionally,it can be rapidly reversed by flumazenil.The clinical research progress on remazolam in the areas of procedural sedation,induction,and maintenance of general anesthesia were reviewed to provide references for its clinical application.

Objective:To investigate the changes of serum miR-210, miR-30a and miR-16 in patients with type 2 diabetes mellitus (T2DM) combined with osteoporosis (OP) and to analyze the influencing factors.Methods:Eighty patients with T2DM admitted to our hospital from May. 2019 to May. 2022 were prospectively selected for the study, and the patients were divided into 42 patients with T2DM combined with OP and 38 patients with T2DM without OP according to whether the patients were combined with OP. Another 40 cases were selected as the control group during the same period of physical examination. We compared the differences of miR-210, miR-30a and miR-16 among the groups, and analyzed the diagnostic value of miR-210, miR-30a and miR-16 on T2DM combined with OP by ROC curve, and analyzed the influencing factors of T2DM combined with OP by binary logistic regression. miR-210, miR-30a and miR-16 were analyzed by Spearman correlation. Spearman correlation analysis of miR-210, miR-30a, miR-16 and T2DM combined OP related indexes.Results:The differences of miR-210, miR-30a, and miR-16 were statistically significant between groups ( F=24.13, 62.69, 307.26, P<0.05), and the control group < T2DM uncomplicated OP group < T2DM combined OP group ( P<0.05). ROC curve analysis showed that miR-210, miR-30a, and miR-16 The AUCs for the diagnosis of T2DM combined with OP were 0.779, 0.854 and 0.973 in order, all of which had some accuracy in diagnosis. Binary logistic multi-factor regression analysis showed that the duration of T2DM disease, glycosylatedhemoglobin (HbA1c), type I collagen carboxy-terminal peptide β special series (β-CTX) were all risk factors for T2DM combined with OP ( P<0.05), body mass index (BMI. BMI, parathyroid hormone (PTH), 25-hydroxyvitamin D3monohydrate (25 (OH) D3), bone Gla protein (BGP), bone mineral density (BMD) were all risk factors for T2DM combined with OP ( P<0.05). Spearman correlation analysis showed that miR-210, miR-30a and miR-16 were negatively correlated with BGP and BMD ( r=-0.668/-0.592/-0.599, -0.671/-0.609/-0.593) and positively correlated with β-CTX ( r=0.670/0.603/0.605) . Conclusion:miR-210, miR-30a and miR-16 are highly expressed in the serum of patients with T2DM combined with OP, and the duration of T2DM, HbA1c and β-CTX were all risk factors for T2DM combined with OP, and BMI, PTH, 25 (OH) D3, BGP and BMD are all protective factors for T2DM combined with OP.

Objective:To apply CBL combined with PBL based on SMART (specific, measurable, attainable, relevant and time-based) principle in nursing practice teaching in radiotherapy.Methods:A total of 100 nurses who performed nursing practice in the Department of Radiotherapy in Guangdong Provincial Hospital of Traditional Chinese Medicine from May 2016 to May 2020 were selected as the research objects. They were divided into a control group and a study group according to their admissions, with 50 people in each group. The study group used CBL combined with PBL teaching based on SMART principle, and the control group used traditional practice teaching. After the clinical practice, the two groups were assessed on theoretical and clinical practice skills, and the two groups' teaching satisfaction and teaching effects were evaluated through seminars and questionnaire surveys. SPSS 22.0 was used for t test and chi-square test. Results:The theoretical and clinical practice performance assessment scores of the practical nurses in the study group were higher than those in the control group, with statistically significant differences ( P<0.001). The teaching satisfaction rate of the practice nurses in the control group was 62.00% (31/50), and that of the practice nurses in the study group was 96.00% (48/50), with significant differences ( P<0.001). In terms of improving independent learning ability, information acquisition and problem analysis ability, improving clinical thinking ability, mobilizing learning enthusiasm, enhancing teamwork ability, nurse-patient communication ability, and recognition of innovation ability, the teaching satisfaction of the research group was higher than that of the control group. Conclusion:The application of SMART principle in teaching has the advantages of clear goals and quantifiable evaluation. The combination of CBL and PBL based on SMART principle can help to improve the mastery of theoretical and practical skills of radiotherapy practice nurses, and achieve satisfactory teaching results.

Objective: To explore the prevalence of attention deficit hyperactivity disorder(ADHD) and its associated factors among college students in Changsha, so as to provide evidence of for effecive intervention of ADHD. Methods: A total of 8 080 students from two universities in Changsha were investigated with self-designed general situation questionnaire, Chinese version of Wende Utah adult rating scale for ADHD, World Health Organization (WHO) Adult ADHD Scale (ASRS) and Chinese Internet Scale (CIAS-R). Results: The self-report rate of ADHD was 7.2%. Single factor analysis showed that the incidence of ADHD in gender, physical and mental illness history, family history of mental illness, relationship with parents, smoking, drinking and Internet access were statistically significont (χ2=17.68, 22.25, 43.15, 20.73, 18.61, 43.05, 22.81, 53.55, 719.65, P<0.05). Multivariate Logistic analysis showed that the younger the age, mental illness, drinking, the higher the degree of Internet dependence were positively correlated with ADHD, while gender (female) and harmony with father were negatively correlated with ADHD. Conclusion ADHD in college students can t be ignored. There are many influencing factors, suffering from mental illness, drinking and Internet addiction.

L-2-aminobutyric acid (L-ABA) is an important chemical raw material and chiral pharmaceutical intermediate. The aim of this study was to develop an efficient method for L-ABA production from L-threonine using a trienzyme cascade route with Threonine deaminase (TD) from Escherichia. coli, Leucine dehydrogenase (LDH) from Bacillus thuringiensis and Formate dehydrogenase (FDH) from Candida boidinii. In order to simplify the production process, the activity ratio of TD, LDH and FDH was 1:1:0.2 after combining different activity ratios in the system in vitro. The above ratio was achieved in the recombinant strain E. coli 3FT+L. Moreover, the transformation conditions were optimized. Finally, we achieved L-ABA production of 68.5 g/L with a conversion rate of 99.0% for 12 h in a 30-L bioreactor by whole-cell catalyst. The environmentally safe and efficient process route represents a promising strategy for large-scale L-ABA production in the future.
Aminobutyrates ; chemical synthesis ; Bacillus thuringiensis ; enzymology ; Candida ; enzymology ; Escherichia coli ; enzymology ; Formate Dehydrogenases ; metabolism ; Leucine Dehydrogenase ; metabolism ; Threonine ; metabolism ; Threonine Dehydratase ; metabolism

Objective:To explore the application effect of learner-centered, simulation-based training in the clinical operation teaching of anesthesiology.Method:s A total of 20 junior students in anesthesiology from a university from 2016 to 2017 were included. In this study, the training of three simulation operations, namely endotracheal intubation, central venous puncture, and arterial catheterization was set up. The self-confidence, operational knowledge, and operational skills before and after the training were assessed through Likert-type questionnaires, multiple-choice tests, and procedural checklists, respectively. Data analyses were performed with the SPSS 16.0. The Wilcoxon signed rank test was applied to compare the self-confidence scores before and after training and the paired t-test was adopted to compare the knowledge and operation scores before and after training.Result:s The students' self-confidence, knowledge and operation scores of the three operations were significantly improved after training ( P<0.05), with the highest increase in the central venous puncture operation score [(7.55±2.40) vs. (15.55±1.84), P<0.01]. The teaching satisfaction survey showed that 95% of the students felt that the training course was conducive to the operation skills learning and were satisfied with the course of this training. Conclusion:The learner-centered, simulation-based training can help the clinical students master the operating skill points quickly and skillfully, and promote the individualized and standardized implementation of the clinical operation skills, thereby improving the clinical teaching effect.

After more than 30 years of exploration and development, undergraduate anesthesiology teaching in China has formed a relatively large teaching scale in the whole country. However, the teaching mode and cultivation mechanism are not yet fully mature, which still has room for improving relevant curriculum structures. This study has investigated the current situation of undergraduate anesthesiology teaching in 38 medical colleges, summarized characteristics of teaching programs and curriculum design, and proposed relevant improvement suggestions.

This study aimed to obtain the first national estimate of the prevalence of autism spectrum disorder (ASD) in Chinese children. We targeted the population of 6 to 12-year-old children for this prevalence study by multistage convenient cluster sampling. The Modified Chinese Autism Spectrum Rating Scale was used for the screening process. Of the target population of 142,086 children, 88.5% (n = 125,806) participated in the study. A total of 363 children were confirmed as having ASD. The observed ASD prevalence rate was 0.29% (95% CI: 0.26%-0.32%) for the overall population. After adjustment for response rates, the estimated number of ASD cases was 867 in the target population sample, thereby achieving an estimated prevalence of 0.70% (95% CI: 0.64%-0.74%). The prevalence was significantly higher in boys than in girls (0.95%; 95% CI: 0.87%-1.02% versus 0.30%; 95% CI: 0.26%-0.34%; P < 0.001). Of the 363 confirmed ASD cases, 43.3% were newly diagnosed, and most of those (90.4%) were attending regular schools, and 68.8% of the children with ASD had at least one neuropsychiatric comorbidity. Our findings provide reliable data on the estimated ASD prevalence and comorbidities in Chinese children.

OBJECTIVETo observe the kinetic change that reflects joint loading in different planes during stair climbing in knee osteoarthritis (KOA) after electroacupuncture (EA) by three-dimensional motion analysis, so as to provide reference for its biomechanical mechanism treated with acupuncture.

METHODSForty KOA patients, in accordance with the random number table, were assigned into an observation group and a control group, 20 cases in each one and finally 18 cases completed. Acupoints in the observation group were Neixiyan (EX-LE 4), Dubi (ST 35), Yanglingquan (GB 34), Yinlingquan (SP 9), Xuehai (SP 10), Liangqiu (ST 34) and Zusanli (ST 36); points in the control groups were located about 2 cm next to the above acupoints with shallow acupuncture. EA was connected at Neixiyan (EX-LE 4) and Yinlingquan (SP 9), Liangqiu (ST 34) and Yanglingquan (GB 34). The frequency was 2 Hz with continuous wave in the observation group and there was no current in the control group for the corresponding points. All the treatment was given for 3 weeks, totally 11 times. Climbing stairs gait was measured before and after treatment. Velocities and kinetic parameters during ascending and descending stairs were analyzed, including flexion and extension peak torques of hip, knee, ankle on the vertical plane, external knee adduction moment on the coronal plane.

RESULTSAfter treatment in the observation group, velocities during ascending and descending stairs significantly increased (<0.05,<0.01); maximal ankle plantar flexor moments during ascending and descending stairs and the second peak external knee adduction moment (PEKAM2) during ascending stairs significantly increased (<0.05,<0.01). After treatment in the control group, the first peak external knee adduction moment (PEKAM1) and PEKAM2 during descending stairs were less than those before treatment (<0.05,<0.01). In the observation group, the difference value (DV) of velocity before and after treatment was positively correlated to DV in the torque of ankle plantar flexors during ascending stairs in the observation group (=0.598，<0.01). Excluding the impact of velocity, the DV of the maximal torque of ankle plantar flexors during ascending stairs didn't show difference in the observation group (>0.05).

CONCLUSIONEA can increase the velocities of ascending and descending stairs of KOA patients. It improves the loading capacity of knee joint on both sagittal and coronary planes. But its effect during ascending may be correlated with the increase of velocity. The mechanism of different effects between EA and minimal acupuncture on joint moments is still unclear and warrants further study.