To investigate the expression and significance of cell surface receptor signaling lymphocyte activation molecule family member 7 (SLAMF7) in normal intestinal tissues and intestinal inflammatory tissues of mice.

Wumei Pills is mostly used to treat ulcerative colitis, irritable bowel syndrome, intestinal polyps, chronic atrophic gastritis, gastroesophageal reflux disease, gastric cancer and other digestive system diseases with its modified formula or combination with Western medicine. Wumei Pills can play a role in treating digestive system diseases through antioxidant stress, Inhibiting inflammatory response, regulating intestinal flora, anti-tumor, promoting intestinal mucosal repair, etc. Most of the related research on the mechanism of Wumei Pills in the treatment of digestive system diseases were studied in a single pathway. The relationship between other mechanisms and pathways is worthy of further exploration. In addition, the current research mostly focuses on the lower digestive system diseases with diarrhea as the main manifestation, and there is still room for research and reporting in the treatment of upper digestive system diseases.

Objective:To investigate the influences of Neferine(Nef)on inflammatory injury in nephrotic syndrome(NS)rats by regulating the MAPK/NF-κB pathway.Methods:SD rats were separated into control check group(CK group),Model group,low-dose Nef group(Nef-L group,2.5 mg/kg),high-dose Nef group(Nef-H group,5 mg/kg),prednisone acetate group(PA group,6.3 mg/kg),Anisomycin(MAPK agonist)group(5 μmol/L),Nef-H+Anisomycin group(5 mg/kg+5 μmol/L),with 12 rats in each group.Except for the CK group,all other groups were injected with doxorubicin through the tail vein to induce the NS rat model.Rats in CK group were injected with an equal volume of normal saline through the tail vein at the same time.After successful modeling,dosing treatment was performed once a day for 4 weeks.Detected 24-hour urine protein content,serum creatinine(Scr),albumin(ALB),urea nitro-gen(BUN)levels,renal tissue pathology,and levels of TNF-α,IL-6,and IL-1β in renal tissue;TUNEL staining was performed to detect cell apoptosis in rat kidney tissue;Western blot was performed to detect the expression of p-p38,p-JNK,p-ERK1/2 and p-NF-κB p65 proteins in rat kidney tissue.Results:Compared with CK group,Model group had severe renal tissue pathological damage,the 24 h urinary protein,Scr,BUN,TNF-α,IL-6,IL-1β,apoptosis rate,p-p38,p-JNK,p-ERK1/2,p-NF-κB p65 protein expressions were increased,while ALB level was decreased(P<0.05);compared with Model group,the renal tissue pathological damage of rats in Nef-L group,Nef-H group and PA group were severe,the 24 h urinary protein,Scr,BUN,TNF-α,IL-6,IL-1β,apoptosis rate,p-p38,p-JNK,p-ERK1/2,p-NF-κB P65 protein expressions were decreased,while ALB level was increased,the renal tissue pathological damage in the Anisomycin group was aggravated,the 24 h urinary protein,Scr,BUN,TNF-α,IL-6,IL-1β,apoptosis rate,p-p38,p-JNK,p-ERK1/2,p-NF-κB p65 protein expressions were increased,while ALB level was decreased(P<0.05);Anisomycin attenu-ated the effects of high doses of Nef on NS rats.Conclusion:Nef may alleviate the inflammatory injury in NS rats by inhibiting MAPK/NF-κB signaling pathway.

This article reports a case of adrenal tumor in a reproductive-aged woman with hyperandrogenism misdiagnosed as polycystic ovary syndrome. The patient presented with menstrual disorder, elevated testosterone levels, and polycystic ovaries, initially diagnosed as polycystic ovary syndrome. Menstrual regularity was achieved after intermittent use of ethinylestradiol/cyproterone acetate and progesterone. Five years later, during a physical examination, a mass was discovered in the right adrenal gland, accompanied by elevated testosterone levels, polycystic ovaries, obesity, and insulin resistance. Laparoscopic resection of the right adrenal tumor was performed, and the pathological diagnosis was adrenal cortical oncocytoma. Postoperatively, menstrual regularity was restored, and testosterone levels normalized. This case highlights the importance of considering adrenal tumors secreting androgens in patients clinically diagnosed with polycystic ovary syndrome, and emphasizes the need for regular adrenal imaging follow-up.

Objective:To explore the effect of information decision aid tool guided by decision coach in breast cancer patients.Methods:From January to December 2021, a total of 134 breast cancer patients in General Oncology Surgery Department of Xinxiang Central Hospital were enrolled using the convenient sampling method, and divided into the control group and the observation group with 67 cases each. The control group received regular decision aid intervention, and the observation group conducted information decision aid tool guided by decision coach, lasting for 1 week. The decision difficulty, decision preparation, decision regret and decision participation satisfaction were compared between the two groups.Results:After intervention, the decision difficulty scores of the two groups decreased, and the score of the observation group was lower than that of the control group, and the difference was statistically significant ( P<0.05) . The scores of decision preparation and decision participation satisfaction of the observation group were higher than those of the control group, while decision regret score was lower than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The information decision aid tool guided by decision coach can reduce decision difficulty and decision regret, promote decision preparation, and improve decision participation satisfaction.

Objective:To explore the effect of remote management based on patient-reported outcomes (PROs) monitoring in patients with breast cancer undergoing chemotherapy.Methods:From September 2020 to March 2021, a total of 134 patients with breast cancer undergoing chemotherapy in the First Affiliated Hospital of Zhengzhou University were selected as research objects by the convenient sampling method, and were divided into the control group and the observation group using the random number table method, with 67 cases in each group. The control group received routine care, while the observation group implemented remote management based on PROs monitoring on the basis of the control group, lasting for 3 months. Quality of life and psychological distress were compared between the two groups.Results:After intervention, the total scores and scores of all dimensions of quality of life of the observation group were higher than those of the control group, and the differences were statistically significant ( P<0.05). After intervention, the scores of psychological distress degree of the observation group was lower than that of the control group, and the number of patients with psychological distress score greater than 4 points was less than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Remote management based on PROs monitoring can improve quality of life of patients, alleviate their psychological distress, which is worthy of clinical promotion and application.

Objective:To analyze the risk factors of bronchopulmonary dysplasia(BPD)in very preterm infants(VPI), and to provide scientific basis for the prevention and treatment of BPD in VPI.Methods:A prospective multicenter study was designed to collect the clinical data of VPI in department of neonatology of 28 hospitals in 7 regions from September 2019 to December 2020.According to the continuous oxygen dependence at 28 days after birth, VPI were divided into non BPD group and BPD group, and the risk factors of BPD in VPI were analyzed.Results:A total of 2 514 cases of VPI including 1 364 cases without BPD and 1 150 cases with BPD were enrolled.The incidence of BPD was 45.7%.The smaller the gestational age and weight, the higher the incidence of BPD( P<0.001). Compared with non BPD group, the average birth age, weight and cesarean section rate in BPD group were lower, and the incidence of male infants, small for gestational age and 5-minute apgar score≤7 were higher( P<0.01). In BPD group, the incidences of neonatal respiratory distress syndrome(NRDS), hemodynamically significant patent ductus arteriosus, retinopathy of prematurity, feeding intolerance, extrauterine growth restriction, grade Ⅲ~Ⅳ intracranial hemorrhage, anemia, early-onset and late-onset sepsis, nosocomial infection, parenteral nutrition-associated cholestasis were higher( P<0.05), the use of pulmonary surfactant(PS), postnatal hormone exposure, anemia and blood transfusion were also higher, and the time of invasive and non-invasive mechanical ventilation, oxygen use and total hospital stay were longer( P<0.001). The time of starting enteral nutrition, cumulative fasting days, days of reaching total enteral nutrition, days of continuous parenteral nutrition, days of reaching 110 kcal/(kg·d) total calorie, days of reaching 110 kcal/(kg·d) oral calorie were longer and the breastfeeding rate was lower in BPD group than those in non BPD group( P<0.001). The cumulative doses of amino acid and fat emulsion during the first week of hospitalization were higher in BPD group( P<0.001). Multivariate Logistic regression analysis showed that NRDS, invasive mechanical ventilation, age of reaching total enteral nutrition, anemia and blood transfusion were the independent risk factors for BPD in VPI, and older gestational age was the protective factor for BPD. Conclusion:Strengthening perinatal management, avoiding premature delivery and severe NRDS, shortening the time of invasive mechanical ventilation, paying attention to enteral nutrition management, reaching whole intestinal feeding as soon as possible, and strictly mastering the indications of blood transfusion are very important to reduce the incidence of BPD in VPI.

Objective:To study the effects of total flavonoids from Baeckea frutescens on the proliferation, migration, invasion, apoptosis and cell morphology changes of cervical cancer SiHa cells.Methods:Cervical cancer SiHa cells were treated with different concentrations of total flavonoids from Baeckea frutescens. CCK8 method was used to detect the proliferation and 50% inhibiting concentration (IC 50) of SiHa cells in vitro. The control group without drug treatment and the experimental group with drug concentration IC 50 were set. Transwell migration and invasion experiments were used to detect the changes of cell migration and invasion ability in vitro in the experimental group and the control group. Laser scanning confocal microscope was adopted for observing the morphological changes of apoptosis in the experimental group and the control group. Flow cytometry was used to detect the apoptosis rates of the experimental group and the control group. Results:Total flavonoids from Baeckea frutescens inhibited the proliferation of cervical cancer SiHa cells in a concentration-dependent manner. After 48 hours of action, the IC 50 value was 110.8 mg/L. In the migration experiment, the number of transmembrane cells in the control group was 644.00±10.54 and the number of transmembrane cells in the experimental group was 266.00±5.57, with a statistically significant difference ( t=54.942, P<0.001). In the invasion experiment, the number of transmembrane cells in the control group was 298.00±14.36, and the number of transmembrane cells in the experimental group was 85.00±8.62, with a statistically significant difference ( t=38.247, P<0.001). Laser scanning confocal microscope observation showed that in the experimental group, the cell membrane crumpled and lost its original morphology, and the nucleus showed typical apoptotic morphologies such as fragments of different sizes and irregular shapes, and nuclear edge aggregation; but no apoptotic cells were observed in the control group. Flow cytometry showed that the apoptosis rate in the control group was (2.95±1.36)%, and the apoptosis rate in the experimental group was (27.54±1.94)%, with a statistically significant difference ( t=-17.949, P<0.001). Conclusion:Total flavonoids from Baeckea frutescens have obvious inhibitory effects on the proliferation, migration and invasion of cervical cancer SiHa cells cultured in vitro, and promote their apoptosis.

The role of Nod-like receptor pyrin domain 3 (NLRP3) inflammasome and pyroptosis in bronchial asthma (asthma) has attracted increasing attention in recent years.The activation of NLRP3 leads to the recruitment and activation of Cysteinyl aspartate specific proteinase-1 and the release of interleukin-1β(IL-1β) and interleukin-18(IL-18), which aggravates the airway inflammation in asthma.Meanwhile, NLRP3 further results in the clea-vage of Gasdermin D and pyroptosis.Finally, the cell damage stimulates the airway remodeling of asthma.This article focused on the mechanisms of NLRP3 inflammatory response and pyroptosis as well as its role in asthma.

Objective: To assess the prognostic influencing factors of acute hearing loss after posterior circulation ischemic stroke. Methods: The clinical data of 50 patients with acute hearing loss caused by posterior circulation ischemic stroke in Dalian Central Hospital Affiliated to Dalian Medical University from January 2016 to December 2018 were retrospectively analyzed. Patients were followed up for 2 to 37 months, with an average of 30 months. At the last follow-up, hearing recovery was assessed. Patients with complete and partial improvement of hearing were classified as hearing improvement group, and patients with no improvement of hearing were classified as hearing without improvement group. Results: There was 32 cases in hearing improvement group and 18 cases in hearing without improvement group. There were no statistical differences in gender composition, age, smoking, diabetes mellitus, hypertension, hyperlipidemia, history of stroke/temporary ischemic attack (TIA), onset to treatment time, hearing loss degree, hearing and vertigo between 2 groups (P>0.05). The proportion of ≥ 2 risk factors in hearing without improvement group was significantly higher than that in hearing improvement group: 17/18 vs. 65.6% (21/32), and there was statistical difference (P<0.05). Conclusions Acute hearing loss caused by posterior circulation ischemic stroke has a good prognosis, but the patients with multiple risk factors for stroke have poor hearing recovery.