Objective To investigate the effects of citicoline sodium and Ureicrin on the expression of miR-17-5p and miR-29b in patients with ischemic stroke.Methods A total of 100 patients with ischemic stroke treated in the hospital were divided into control group and combination group by random table method.From February 2023 to February 2024,they were respectively given Eurecrine monotherapy and citicoline sodium and eurecrine combination therapy.Cerebral blood perfusion indexes,inflammatory factors,oxidative stress,miR-17-5p,miR-29b levels and clinical efficacy were detected in the two groups,and adverse reactions were statistically recorded.Results There was no significant difference in CBF,CBV,hs-CRP,miR-29b,IL-6,TNF-α and miR-17-5p between the two groups before treatment(P>0.05).After treatment,the levels of CBF,CBV,miR-29b,SOD and GSH-Px increased,while the levels of hs-CRP,IL-6,TNF-α,miR-17-5p and MDA decreased,and the levels of CBF,CBV,miR-29b,SOD and GSH-Px in the combined group were higher than those in the control group.The levels of hs-CRP,TNF-α,IL-6,miR-17-5p and MDA were significantly different from those of the control group(P<0.05).The incidence of adverse reactions and the total effective rate of treatment in combination group were higher than those in control group(P<0.05),and the adverse reactions were mild and tolerable.Conclusion The combination of citicoline sodium and Ureicrin can improve the clinical efficacy and prognosis of patients.

【Objective】 To investigate the practice and benefit of national standardized management of type 2 diabetes in Yulin City. 【Methods】 We recruited the adult type 2 diabetes patients who sought medical help at our hospital from May 2020 to October 2022 as subjects. We collected their basic information (sex and age); measured height, weight, waist and hip circumference, and blood pressure; calculated body mass index (BMI); and detected blood glucose, c-peptide, HbA1c, biomarkers, urinary microalbumin, sensory nerve conduction velocity of lower limbs, ABI, and subcutaneous and visceral fat at the time of MMC recruited and the end of six months. T test and Mann-Whitney U rank sum test were used for measurement data and χ² test or Fisher’s exact probability method for counting data to analyze the data. 【Results】 After 6 months, the levels of fasting blood glucose, postprandial blood glucose, HbA1c, and visceral and subcutaneous fat in all the patients decreased, but the level of fasting c-peptide increased compared with the baseline (all P<0.05). Secondly, compared with the baseline, the control rate of HbA1c (35.21% vs. 13.71% ) and the comprehensive control rate (13.97% vs. 7.26% ) were both significantly increased at six months (P<0.05). Thirdly, after 6 months, the levels of fasting blood glucose, postprandial blood glucose, HbA1c, TG, TC, and UA were decreased more, while the fasting c-peptide and postprandial c-peptide were increased more in the patients of the HbA1c standard group (HbA1c<7% ) than those of the non-standard group. 【Conclusion】 The multiple benefits of blood glucose, blood lipid, uric acid and islet function can be achieved by taking type 2 diabetes patients into MMC. Meanwhile, the rates of HbA1c control and comprehensively reaching the standard are significantly increased. Therefore, MMC can explore a new way for the management of type 2 diabetic patients in this area.

ObjectiveTo analyze the polarized light microscopic characteristics， the composition of physical phases and their relative contents of Maifanitum from different origins， and to establish the Fourier characteristic fingerprint of Maifanitum powder crystals by X-ray diffraction（XRD）. MethodA total of 26 batches of Maifanitum samples were selected， and the microscopic characteristics of the sample powders and grinding flakes were observed by polarized light microscopy under single polarized light and orthogonal polarized light， and the main phase compositions and their relative contents were analyzed by powder crystal XRD technique， and the XRD Fourier characteristic fingerprint of Maifanitum was established. The incident light source of XRD was Cu target Kβ radiation， the light tube voltage and light tube current were 40 kV and 40 mA， respectively， the divergence slit was 1°， the scattering slit was 1°， the receiving slit was 0.2 mm， the scanning speed was 5°·min^-1 with continuous scanning and scanning range of 5-90°（2θ）， and the step length was 0.02°. ResultThe polarized light micrographs of powders and grinding flakes of Maifanitum were obtained， and the main phases were plagioclase， potassium feldspar and quartz， and a few samples also contained illite， pyrite， iron dolomite， calcite， iron amphibole and chlorite， etc. The relative total content of feldspar phases was 61.9%-82.4%， and the relative content of quartz was 12.6%-33.6%. The XRD Fourier fingerprint analysis method of Maifanitum with 13 common peaks as the characteristic fingerprint information was established， and the similarity calculated by the mean correlation coefficient method was 0.920 9-0.997 7， the similarity calculated by the mean angle cosine method was 0.940 5-0.998 4， the similarity calculated by the median correlation coefficient method was 0.921 1-0.997 5， and the similarity calculated by the median angle cosine method was 0.947 5-0.998 2. ConclusionThe polarized light microscopic identification characteristics of Maifanitum are mainly plagioclase， quartz and potassium feldspar， and the technique of powder crystal XRD Fourier fingerprint analysis can be used for the identification of Maifanitum.

To analyze the methodological and reporting quality of systematic reviews of prediction models published in Chinese journals, with the aim of providing reference for enhancing the overall quality of Chinese systematic reviews of prediction models.

Objective:To construct a recombinant herpes simplex virus 2 (HSV-2) expressing enhanced green fluorescent protein (EGFP) using clustered, regularly interspaced, short palindromic repeat/CRISPR-associated nuclease 9 (CRISPR/Cas9) technology.Methods:Four strategies for inserting exogenous EGFP gene into HSV-2 genome using CRISPR/Cas9 technology were designed: (1) conventional homology-directed repair: circular two homology arm donor-mediated gene knock-in; (2) linearized single homology arm donor-mediated gene knock-in; (3) homology-independent targeted integration; (4) conventional homology-directed repair-mediated by cell lines stably expressing Cas9 and sgRNA.Results:The recombinant virus HSV-2-EGFP was successfully constructed based on the second, the third and the fourth strategies. The second strategy was the most efficient, followed by the third and the fourth strategies. The purified recombinant virus could stably express green fluorescent protein in seven passages and shared similar growth characteristics in Vero cells to the parental virus.Conclusions:Linearized single homology arm donor could increase the efficiency of gene knock-in, and cell lines stably expressing Cas9 and sgRNA could increase the efficiency of gene knock-in mediated by homology-directed repair.

Objective:To investigate the effects of genomic location of a foreign gene in Shanghai-191 strain measles virus (MV) vector on gene expression and virus replication.Methods:The nucleotide sequence encoding S1 protein of SARS-CoV-2 was inserted at different positions in MV antigenome (the upstream of the N gene, between P and M genes, between H and L genes), and co-transfected into 293T cells with helper plasmids coding T7 RNA polymerase and N, P, and L proteins, respectively. The transfected cells were lysed and the supernatants were used to infected Vero cells to harvest recombinant viruses. S1 proteins expressed by the recombinant viruses were identified by RT-PCR, indirect immunofluorescence assay, Western blot and ELISA. Growth kinetics of the recombinant viruses were analyzed.Results:Recombinant viruses were failed to be rescued when the S1 protein-coding sequence was cloned into the upstream of N gene. Two recombinant viruses, MV-M-S1 and MV-L-S1, were successfully rescued when cloning the S1 protein-coding sequence into the intergenic region between P and M genes, or H and L genes, and could express S1 protein. MV-M-S1 expressed more S1 protein than MV-L-S1, but the titer of MV-M-S1 was lower.Conclusions:Inserting a foreign gene at different positions in the MV genome might have different effects on gene expression and virus replication. This study provided reference for the subsequent construction of MV vector.

Objective:To analyze the thalassemia screening and genotyping in Southwest Guizhou Autonomous Prefecture (referred it as Qianxinan Prefecture), this essay provides the theoretical reference for clinical diagnosis of thalassemia and suspicious cases.Methods:The pregnant women, spouses and neonates who were screened for thalassemia gene in Qian Xi Nan People's Hospital from January 2016 to December 2020 were selected as the research subjects, and peripheral blood or umbilical cord blood samples were collected to extract DNA. The gap-polymerase chain reaction (Gap-PCR) and next-generation sequencing (NGS) technology were used to screen thalassemia, and ArcMap 10.8 software was adopted to map the local spatial distribution of thalassemia based on the screening data.Results:A total of 67 185 cases of people from various regions in Qianxinan Prefecture were screened, and 8 202 cases of thalassemia gene carriers were detected, with a total detection rate of 12.21%. Among them, 5 660 cases of α-thalassemia, with a detection rate of 8.42%; 2 132 cases of β-thalassemia, with a detection rate of 3.17%; 410 cases of αβ complex thalassemia, with a detection rate of 0.61%. In the detection of thalassemia genes, 27 genotypes of α-thalassemia were detected, mainly αα/-α 3.7, accounting for 41.13% (2 328/5 660); 33 genotypes of β-thalassemia were detected, mainly β CD17(A>T)/β A, accounting for 44.09% (940/2 132); 55 genotypes of αβ complex thalassemia were detected, and αα/-α 3.7 complexed β CD17(A>T)/β A dominated, accounting for 21.22% (87/410). There were high incidence areas in the spatial distribution of thalassemia, which were Wangmo County and Ceheng County, and the detection rate was 26.76% (1 438/5 374), 24.39% (1 314/5 387), respectively. Conclusions:The detection rate of thalassemia gene in Qianxinan Prefecture is relatively high, mainly αα/-α 3.7 genotype of α-thalassemia. Wangmo County and Ceheng County are high-incidence areas of thalassemia, and screening efforts should be continued.

Objective:To study the impact of excessive iodide intake during pregnancy and lactation on lipid metabolism in offspring male rats.Methods:Forty-eight six-week-old Wistar rats (half male and half female) were fed adaptively for one week. The cage was closed according to the ratio of male and female 1∶1. The pregnant rats were divided into two groups according to their weight (220-240 g) by random number table. (1) 10 times high iodine (10 HI) intake during pregnancy and lactation until the postnatal day 21 (PN21) of their offspring: pregnant rats were divided into normal iodine group (NI group, drinking deionized water), 10 HI group (drinking potassium iodide solution with iodine content of 2 250 μg/L). Breast milk was used to feed the offspring rats to PN21, and the offspring male rats were taken as the research subjects, with 6 rats in each group. (2) 100 times high iodine (100 HI) intake during pregnancy and lactation to the offspring postnatal day 120 (PN120): pregnant rats were divided into NI group (drinking deionized water) and 100 HI group (drinking potassium iodide solution with iodine content of 24 750 μg/L). After feeding the offspring rats with breast milk until PN21, the offspring were continued to drink potassium iodide solution with the same iodine content as the mother's to PN120. The offspring male rats were taken as the research subjects, with 6 rats in each group. The levels of free triiodothyronine (FT 3), free thyroxine (FT 4), thyrotropin (TSH) in serum, low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and total cholesterol (TC) in serum and liver tissue homogenates were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of cholesterol 7α-hydroxylase (CYP7A), low-density lipoprotein receptors (LDLR), sterol regulatory element-binding protein (SREBP)-1c, malic enzyme (ME) and thyroid hormone receptor β (TRβ) in the liver tissue were measured by real-time quantitative PCR. Results:(1) Effects of 10 HI intake during pregnancy and lactation on PN21 offspring male rats: compared with NI and 10 HI groups, the serum FT 3 [(7.53 ± 0.74), (8.88 ± 0.99) pmol/L], FT 4 [(5.58 ± 0.56), (7.68 ± 0.30) pmol/L], TSH levels [(16.69 ± 1.05), (14.49 ± 0.16) ng/ml] of offspring male rats were statistically significant ( t=- 2.91,-8.76, 3.59, P < 0.05). The levels of LDL-C, TG, TC in serum and liver of offspring male rats of 10 HI group were significantly lower than those of NI group ( t=3.28, 8.71, 3.44, 3.70, 3.49, 2.74, P < 0.05). The differences of mRNA expression levels of LDLR, ME, SREBP-1c in the liver of PN21 offspring male rats of 10 HI and NI groups were statistically significant ( t=- 3.50,-3.92, 5.58, P < 0.05). Among them, the levels of LDLR and ME in 10 HI group were higher than those in NI group, while the level of SREBP-1c in 10 HI group was lower than that in NI group. There no significant difference in CYP7A and TRβ mRNA levels between the two groups ( t=- 2.44, 3.20, P > 0.05). (2) Effects of 100 HI intake during pregnancy and lactation on PN120 offspring male rats: there were significant differences in serum FT 3, FT 4 and TSH levels of offspring male rats between 100 HI and NI groups ( t=- 4.39,-3.19, 4.72, P < 0.05). The levels of serum FT 3 and FT 4 in 100 HI group were lower than those in NI group, and the level of TSH in 100 HI group was higher than that in NI group ( P < 0.05). Compared with NI group, the serum and liver LDL-C, TG and TC levels in the offspring male rats of 100 HI group were significantly higher ( t=4.49, 12.85, 16.62, 4.35, 11.04, 16.01, P < 0.05). The differences of CYP7A, LDLR, ME, TRβ and SREBP-1c mRNA levels in liver of PN120 offspring male rats of 100 HI and NI groups were statistically significant ( t=26.40, 54.85,-10.98, 32.52, 10.50, P < 0.05). Among them, the CYP7A, LDLR, ME and TRβ mRNA levels in 100 HI group were lower than those of NI group, while the SREBP-1c mRNA level was higher than that of NI group ( P < 0.05). Conclusions:10 HI intake during pregnancy and lactation to the offspring male rats PN21 showed the serological changes of hyperthyroidism, the levels of blood lipids and liver lipids decreased, the levels of LDLR and ME mRNA increased, and SREBP-1c mRNA decreased in liver. However, 100 HI intake during pregnancy and lactation to the offspring male rats PN120 showed serological changes of hypothyroidism, the levels of blood lipids and liver lipids increased, the levels of CYP7A, LDLR, ME mRNA decreased, and SREBP-1c mRNA increased in liver.

Objective:To explore the effects of the "Internet +" hospital-family standardized feeding management model in premature infants.Methods:From May 2019 to November 2020, 240 moderately-risk and low-risk premature infants hospitalized at the Department of Neonatal Medicine in Henan Children's Hospital were selected by convenience sampling and divided into a control group and an experimental group according to the random number table and order of admission, with 120 infants in each group. Infants in the control group received standardized nursing, while infants in the experimental group underwent "Internet +" hospital-family standardized feeding management on this basis. The weight, body length and head circumference of the two groups of premature infants were measured at the age of 40 weeks, 6 months, and 12 months after correction; the hemoglobin and serum ferritin levels were detected, and the incidence of anemia and iron deficiency were observed at the age of 6 months and 12 months after correction; the readmission rate, neurological function, and parental satisfaction with care were investigated at the age of 12 months after correction.Results:At the age of 40 weeks after correction, there was no significant difference in weight, body length, and head circumference between the experimental and control groups ( P>0.05) . At the age of 6 and 12 months after correction, the weight, body length, and head circumference were larger in the experimental group than in the control group, and the difference was statistically significant ( P<0.01) . At the age of 6 months after correction, there was no significant difference in the hemoglobin and serum ferritin levels between the two groups of premature infants ( P>0.05) . At the age of 12 months after correction, the hemoglobin and serum ferritin levels in the experimental group was improved compared with the control group; The incidences of anemia and iron deficiency in the experimental group were lower than those in the control group; the readmission rate in the experimental group was lower than that in the control group, and the neurological function score was higher than that in the control group, with statistically significant difference ( P<0.05) ; parents of premature infants in the experimental group had better nursing satisfaction than the control group, and the difference was statistically significant ( P<0.05) . Conclusions:The "Internet +" hospital-family standardized feeding management model can improve the nutritional status of premature infants, promote their catch-up growth, improve prognostic outcomes, and elevate the satisfaction of parents of premature infants with continuous care.

Objective:To investigate the relationship between gene polymorphism of osteoprotegerin (OPG) and coal-burning endemic fluorosis in Guizhou Province.Methods:In 2018 and 2019, a case-control study was conducted in Bijie City, a typical coal-burning endemic fluorosis area in Guizhou Province, and 260 cases of coal-burning endemic fluorosis patients were selected as case group. According to the "Diagnostic Criteria for Endemic Skeletal Fluorosis" (WS 192-2008), the case group was divided into severe group (130 cases) and mild group (130 cases). At the same time, 130 cases without dental fluorosis and skeletal fluorosis symptoms were chosen as control group in Changshun County, a non-coal-burning endemic fluorosis area in Guizhou Province. Whole blood genomic DNA was extracted, and real-time fluorescence quantitative PCR with TaqMan-MGB probe was utilized to type the OPG gene rs2460985, rs2073618, rs6469804 and rs6993813 four single nucleotide polymorphism (SNP) loci of all samples, and genetic model analysis was performed to compare the frequency distribution of its alleles, genotypes and constructed haplotypes in control, mild and severe groups. Results:By Hardy-Weinberg equilibrium test, the genotype frequencies of the four SNP loci all reached genetic balance in control, mild and severe groups ( P > 0.05). There was a statistically significant difference in the genotype frequency of OPG gene rs6469804 locus among the three groups (χ 2 = 10.615, P < 0.05), and the difference in the genotype frequency of this locus between the control group and the severe group was statistically significant (χ 2 = 6.784, P < 0.05). The results of genetic model analysis showed that in comparison between the control group and the severe group, the optimal genetic model of rs6469804 locus was the overdominant genetic model, the frequency distribution of genotypes AA + GG and AG in the control group and the severe group was statistically significant [odds ratio ( OR) = 1.94, 95% confidence interval ( CI): 1.16 - 3.23, P < 0.05], genotype AG was a risk factor for coal-burning endemic fluorosis. In comparison between the control group and the mild group, the optimal genetic model of rs2073618 locus was the recessive genetic model, the frequency distribution of genotypes GG + GC and CC in the control group and the mild group was statistically significant ( OR = 3.17, 95% CI: 1.08 - 9.30, P < 0.05), genotype CC was a risk factor for coal-burning endemic fluorosis. In comparison between the control group and the mild group, haplotypes C-C-G-T and T-G-A-C were risk factors for coal-burning endemic fluorosis (adjusted OR = 2.41, 1.98, 95% CI: 1.29 - 4.50, 1.22 - 3.23, P < 0.05); in comparison between the control group and the severe group, haplotype T-G-A-C was a risk factor for coal-burning endemic fluorosis (adjusted OR = 1.87, 95% CI: 1.14 - 3.07, P < 0.05). Conclusion:OPG gene rs6469804 locus genotype AG and rs2073618 locus genotype CC may be risk factors for coal-burning endemic fluorosis.