Objective To explore the protective effects of Acacetin on mouse microglial cells injured by oxygen-glucose deprivation/reperfusion (OGD/R) based on autophagy/ROS/NLRP3 inflammasomes signaling pathway.Methods Mouse microglial cells (BV2) were divided into normal control group,OGD model group and OGD+Acacetin group (10 μmol).After hypoxia for 6 hours and reoxygenation for 24 hours,the viability of BV2 cells was detected by 4-methylazolazolium blue (MTT) method.The death rate of cell was measured by lactate dehydrogenase (LDH) method.Reactive oxygen species (ROS) assay kit was used to measure the intracellular ROS level.The expression of LC3-Ⅱ,LC3-Ⅱ/LC3-Ⅰ,beclin-1,NLRP3,caspase-1 and IL-1β were analyzed by western blot.Results Acacetin increased the survival rate of microglia cells,and reduced the release of LDH and production of ROS.Acacetin also increased the expression of LC3-Ⅱ,and further to decrease the expression of NLRP3,caspase-1 and IL-1 β.Conclusions Acacetin protected against cerebral ischemia-reperfusion injury by attenuating the injury of microglia after OGD/R.Its protective mechanism may be related to inhibiting the production of ROS,activating autophagy,and inhibiting the NLRP3 inflammasome.

Objective To explore the effect of coronavirus disease 2019 (COVID-19) on the frequency of chest CT scan. Methods A retrospective study was conducted to extract information on the number of outpatient, emergency, and inpatient visits and patients who had chest CT imaging examination from January 1 to December 31, 2020 and in the same period in 2019 through the hospital’s medical data platform for analysis, and the chi-square test was used to analyze whether the difference in the proportion of patients who had chest CT imaging examination between 2019 and 2020 was statistically significant. Results The proportion of outpatients and emergency patients with chest CT examination was significantly higher in 2020 than in 2019 (2.48% vs 1.47%, χ² = 581.7, P < 0.000). The proportion of inpatients who underwent chest CT examination was significantly higher in 2020 than in 2019 (35.47% vs 28.01%, χ² = 182.0, P < 0.000). Conclusion Under the COVID-19 epidemic, the proportion of chest CT examination in this hospital in 2020 shows a significant upward trend compared with the same period in 2019, which will increase the collective dose due to medical exposure, and the hospital should pay attention to the determination of the legitimacy of chest CT scan.