Objective: To analyze the occurrence of unintentional injuries among college students and their association with sexual orientation and gender identity, so as to provide a targeted scientific basis for injury prevention measures and intervention strategies. Methods: From October 24 to November 18, 2023, a sample of 1 629 college students from two general universities in Beijing was selected using convenience sampling method. A questionnaire survey was conducted to collect information on the gender identity, sexual orientation and occurrence of unintentional injuries among college students in the past year. The Symptom Checklist-90 (SCL-90), Pittsburgh Sleep Quality Index (PSQI), Childhood Trauma Questionnaire (CTQ), and Delaware Bullying Victimization Scale-Student (DBVS-S) were used to assess mental health, sleep quality, childhood trauma, and dysfunctional impulsivity status. Analyses of sexual orientation and gender identity were conducted. The t-test and Chi square test were used for intergroup comparison,and multivariate Logistic regression analysis was used to examine risk factors for unintentional injuries among college students of different gender identities. Results: The incidence rate of unintentional injuries among college students was 16.94%, with boys (17.08%) being higher than girls (16.90%). Compared with those who did not experience unintentional injuries (5.28± 3.60 , 118.68±41.38), college students who experienced unintentional injuries had poorer sleep quality and mental health status ( 6.38 ±3.93, 135.59±50.96)( t =-3.92, -4.26); the differences in the incidence of unintentional injury among college students with non suicidal self injury, interpersonal violence, childhood trauma, and different sexual orientations and gender identities were all statistically significant ( χ 2=28.75, 75.18, 9.83, 16.20, 4.13) (all P <0.05). Multivariate Logistic regression analysis showed that after adjusting for age, gender and body mass index, non heterosexual orientation increased the risk of unintentional injuries ( OR=1.61, 95%CI =1.09-2.38), whereas existing non suicidal self injury behaviors ( OR=2.10, 95%CI =1.02-4.37) and poorer mental health status ( OR=1.54, 95%CI =1.05-2.27) increased the risk of unintentional injuries among non heterosexual college students (all P <0.05). Conclusions The incidence rate of unintentional injuries among college students is relatively high, with non heterosexual groups having increased risk of unintentional injuries. Mental health status and non suicidal self injury behaviors are important factors related to unintentional injuries among non heterosexual college students.

Monoclonal antibody-based therapy has achieved great success and is now one of the most crucial therapeutic modalities for cancer therapy. The first monoclonal antibody authorized for treating human epidermal growth receptor 2 (HER2)-positive breast cancer is trastuzumab. However, resistance to trastuzumab therapy is frequently encountered and thus significantly restricts the therapeutic outcomes. To address this issue, tumor microenvironment (TME) pH-responsive nanoparticles (NPs) were herein developed for systemic mRNA delivery to reverse the trastuzumab resistance of breast cancer (BCa). This nanoplatform is comprised of a methoxyl-poly (ethylene glycol)-b-poly (lactic-co-glycolic acid) copolymer with a TME pH-liable linker (Meo-PEG-Dlink _m -PLGA) and an amphiphilic cationic lipid that can complex PTEN mRNA via electrostatic interaction. When the long-circulating mRNA-loaded NPs build up in the tumor after being delivered intravenously, they could be efficiently internalized by tumor cells due to the TME pH-triggered PEG detachment from the NP surface. With the intracellular mRNA release to up-regulate PTEN expression, the constantly activated PI3K/Akt signaling pathway could be blocked in the trastuzumab-resistant BCa cells, thereby resulting in the reversal of trastuzumab resistance and effectively suppress the development of BCa.

Objective To study the structures of the plasmids of Klebsiella pneumoniae KF3 at the genome metagenome level througth with whole plasmid DNA sequencing, to analyze the functional genes carried by plasmid and to identify the correlation of resistance and pathogenicity between the plasmids and the host strains. Methods The alkaline lysis method was used to extract plasmids. We constructed the small insert pUC18 library and the large insert Forsmid library, sequenced and used the Phred / Phrap / Consed package to assemble these sequences and gained a complete sequence. The open reading frame(ORFs) were predicted by the Glimmer software and annotated, analyzed the functions of these genes. Results We successfully constructed the pUC18 library and the Fosmid libraries for the plasmid DNA and obtained three circular double-stranded DNA plasmids: pKF3-70 (69 477 bp), pKF3-90 (91 327 bp) and pKF3-147 ( 147 416 bp). There were drug resistant genes, conjugative transfer genes and mobile DNA elements identified on three plasmids. Conclusion The three plasmids of KF3 could be transferred among different strains. It would lead to the dissemination of the resistant genes.

Objective To explore the effect of early pelvis control training on the balance function in patients with hemiplegia. Methods 60 early stroke patients were divided into the treatment group and the control group. The patients in the treatment group accepted the early pelvis control training based on the normal early rehabilitation which was given to the control. They were evaluated with Berg Balance Scale and the Modified Bathel Index before and 2 months after treatment. Results The scores of Berg Balance Scale and the Modified Bathel Index of the treatment group improved more than that of the control group (P<0.05). Conclusion The early pelvis control training can improve the balance dysfunction after hemiplegia effectively.

Objective:To compare the anti tumor activity and immune enhancing effect of crude(PGF) and purified (PGF 1) polysaccharide from Grifola frondosa. Methods:S 180 bearing mice were used as animal model. The effects on tumor weight and immune function were investigated. Results:PGF and PGF 1 showed inhibitory activity on S 180 growth. They could also increase weight of immune organs and improved the phagocytic function, DTH response, lymphocyte proliferation, antibody formation in splenic cells and content of serum HC IgM markedly. PGF was better than PGF 1. Conclusion:Both PGF and PGF 1 can inhibit S 180 tumor growth and enhance immune function in S 180 bearing mice.

Objective: To study the inhibition effect on Sarcoma 180 (S 180 ) and immune enhancing effect of rapeseed peptides (RSP) from enzymolysis of rapeseed protein. Methods: S 180 -bearing mice were used as animal model. The changes of tumor weight, lymphocyte proliferation, antibody in splenic cells and hemolysin were investigated. Results: Compared with cyclophosphamide (Cy), RSP inhibited S 180 growth markedly and enhanced immune function .Conclusion: RSP has potential in enhancing immune function and inhibiting tumor growth, but it deserves for further study.

Objective: To study the effect of rapeseed peptides (RSP) from enzymolysis of rapeseed protein on growth of Sarcoma 180 (S180) cell incubated in vitro and its cell membrane. Methods: S180 cell incubated in vitro was used as model. The change of proliferation of S180 cell, fatty acid and sialic acid in membrane and membrane fluidity of S180 cell were investigated. Results: RSP had inhibitiory ability to S180 cell proliferation and could influence its membrane. Conclusion: RSP had potential in inhibiting tumor growth, so was needed to study further.