Objective:To explore the effects and mechanism of Bushen Tongluo Prescription in mechanical injury of rat endometrium.Method:A total of 60 female SD rats were divided into the blank group, model group, estradiol valerate group, and Bushen Tongluo Prescription group according to the random number table method, with 15 rats in each group. Except for the blank group, the other three groups were used to establish a rat model of endometrial injury using mechanical injury method. After modeling, Bushen Tongluo Prescription group was orally administered with Bushen Tongluo Prescription decoction at a dosage of 2.1 g/kg, the estradiol group was orally administered with estradiol valerate at a dosage of 0.4 mg/kg, and the blank group and model group were orally administered with 0.5% CMC at an equal dosage, once a day, for a total of 8 days. Samples were taken on the 1st, 4th, and 8th day after gastric lavage. The thickness of the endometrium and glands were observed using HE staining, the degree of uterine tissue fibrosis was observed using Masson staining, and VEGF and TGF-β protein and mRNA expressions in uterine tissue were detected using Western blot and fluorescence quantitative PCR.Results:Compared with the Bushen Tongluo Prescription on the first day, the thickness of the endometrium and the number of glands increased on the eighth day ( P<0.05); compared with the model group, the number of glands in the Bushen Tongluo Prescription group increased on the 4th day of administration ( P<0.05), while the fibrotic area of the endometrium decreased on the 8th day of administration ( P<0.05); compared with the model group, on the 8th day of administration, the expression of VEGF protein and TGF-β1 in the Bushen Tongluo Prescription group increased ( P<0.05), and protein expression decreased ( P<0.05); compared with the model group, the group treated with Bushen Tongluo Prescription had TGFβ1 mRNA level on the first day increased ( P<0.05), while the level of TGF-β1mRNA decreased on the 8th day ( P<0.05). Conclusion:Bushen Tongluo Prescription can increase endometrial thickness and receptivity, effectively improve damaged endometrium, anti fibrotic and prevent endometrial adhesion by up-regulating VEGF protein expression and down-regulating TGF-β1 protein and mRNA expression.

Objective:To investigate the psychological state and influencing factors of clinical front-line nurses in response to public health emergencies.Methods:We used stratified cluster random sampling method to survey 1187 front-line clinical nurses from February to March, 2020, using the Psychological Questionnaire for Emergent Events of Public Health.Results:The questionnaire survey showed that the scores for dimensions of depression, neurasthenia, fear, obsessive-anxiety and hypochondria were (0.52±0.02), (0.37±0.01), (0.87±0.02), (0.24±0.01) and (0.25±0.01), respectively. There were statistically significant differences in scores of hypochondriac dimension for clinical nurses at different hospital levels ( F=4.30, P<0.05), and there were statistically significant differences in scores of depression, neurasthenia, obsessive-anxiety and hypochondriac dimension for nurses in different nursing units ( F values were 4.34-11.11, all P<0.05). Multiple linear regression analysis showed that the nursing unit factors had influence on depression, neurasthenia, obsessive-anxiety and hypochondria. Conclusions:Clinical front-line nurses experienced negative psychology such as depression, neurasthenia and fear in public health emergencies. As a manager, it is necessary to strengthen preventive psychological counseling, do a good job in the theory, operation and protection knowledge training for public health emergencies, humanistic care, establish a good organizational atmosphere to maintain the physical and mental health of clinical nurses, and ensure the quality and safety of nursing.

Lung transplantation is the only effective treatment of end-stage lung diseases. Nevertheless, shortage of donor lungs has become increasingly prominent worldwide. A large quantity of patients died while waiting for lung transplantation. Urgent lung transplantation is a prioritized allocation strategy for donor lung transplantation according to the urgency of diseases, aiming to shorten the waiting time for donor lungs and reduce the fatality of patients on the waiting list for lung transplantation. However, no consensus has been reached worldwide on the definition, criteria and application of the terminology of urgent lung transplantation. In addition, the survival and net benefits of lung transplant recipients based on this allocation system are still controversial. On the basis of previous clinical research on urgent lung transplantation, the definition criteria, risk factors, survival outcomes, limitations and optimization measures were explicitly elucidated in this article, aiming to provide theoretical reference for comprehensive evaluation of the feasibility of urgent lung transplantation and further optimizing the allocation system of donor lungs.

This article reviews the concept, classification, screening and evaluation tools of cognitive frailty, and research in chronic diseases of the elderly, with the aim of improving the awareness and understanding of cognitive frailty among medical and nursing staff. It is recommended to further strengthen the research on cognitive frailty in elderly patients with chronic diseases, optimize screening tools, and promote the construction of targeted intervention programs, so as to provide a basis for the research progress of cognitive frailty in elderly chronic diseases in China.

Objective To investigate the mechanism of white matter damage (WMD) and the neuroprotective effect of Xenon on neonates with WMD.Methods Three-day-old SD rat pups (n =96) were randomly divided into the blank control group (n =24),the WMD control group (n =24),the Xenon intervention group A (n =24) and the Xenon intervention group B (n =24) by random number method according to their birth time.WMD rat models were successfully established by giving intraperitoneal injection of lipopolysaccharide(LPS) 0.05 mg/kg combined with carotid artery ligation and hypoxia for 1 hour in the WMD control group and the Xenon intervention groups.In the control group,only 9 g/L saline (0.05 mg/kg) was injected intraperitoneally,while carotid artery ligation and hypoxia were not administered.Rats in Xenon intervention group A and group B were given inhalation of 500 mL/L Xenon for 3 hours at 0 and 2 hours respectively after establishment of the models.Six rats in each group were randomly selected and decapitated at 0,24,48 and 72 hours after the intervention.The brain white matter on the right was analyzed by using HE staining and myelin basic protein(MBP) immunofluorescence staining,and real-time quantitative polymerase chain reaction was used to detect the expressions level of CLIC4 mRNA.Results (1) Brain tissue pathology:compared with the blank control group,the brain white matter on the right of the WMD control group and the Xenon intervention group A and group B had loose and disordered structure,nuclear pyknosis and cytoplasm loosening.However,the lesions in both Xenon intervention group A and group B were significantly less than those in the WMD control group,and there was no significant difference between the Xenon intervention group A and group B.(2) MBP measurement:the number of MBP-positive cells in the brain white matter on the right of WMD control group was significantly lower than that in the blank control group,while compared with WMD control group,they were significantly higher in Xenon intervention group A and group B.(3) CLIC4 mRNA expression level:compared with blank control group,the expressions levels of CLIC4 mRNA at most time point were higher both in the WMD control group and the Xenon intervention group A and group B (all P ＜ 0.05),except the time point 24 h in the Xenon intervention group A.The expressions of CLIC4 mRNA in group A and group B were significantly decreased compared with those in the WMD control group (all P ＜ 0.05).However,there were no significant differences between Xenon intervention group A and group B (P ＞ 0.05).Conclusions The expressions of CLIC4 mRNA in brain tissues on neonatal rats with WMD significantly increased,indicating that the mitochondrial pathway could be one of the pathological processes of WMD.Early Xenon intervention may reduce neonatal WMD by reducing the expression of CLIC4 mRNA,which plays a neuroprotective role.

Objective? To explore the competitive attitude of clinical nurses of Infectious Disease Department and its influencing factors so as to provide a basis for improving nursing management strategies of infectious disease further. Methods? From April 2017 to June 2017, we selected 454 clinical nurses of Infectious Disease Department from 8 Class Ⅲ hospitals in Nantong City as subjects by convenience sampling. The Competitive Attitude Scale (CAS) was used to investigate the score of competitive attitude of clinical nurses of infectious disease department and multiple linear regression was used to analyze its influencing factors. Results? Among 454 clinical nurses of Infectious Disease Department in Nantong City, the scores of benign competition attitude and excessive competition were (45.13±8.17) and (29.57±5.51) respectively. There were statistical differences in the scores of excessive competition among clinical nurses of infectious disease department with different work lives and professional titles (F=3.147,3.556; P＜ 0.05). Multiple linear regression showed that the influencing factors of excessive competition included work lives and professional titles (P＜0.01). Conclusions? Clinical nurses of Infectious Disease Department have the low score of competitive attitude. The work lives and professional titles are the influencing factors of excessive competition. Nursing managers of infectious disease department should establish a reasonable competitive mechanism to allow full paly to benign competition, avoid excessive competition and improve whole competitiveness.

Objective To investigate the core competence, self-efficacy and correlation of geriatric clinical front-line nurses in Nantong medical institutions, and to analyze the influencing factors of the two, so as to provide reference for the formulation of training programs for geriatric clinical front-line nurses. Methods Using convenience sampling method, the geriatric clinical front-line nurses in Nantong medical institutions were investigated by Geriatric Nursing Competency Inventory (GNCI) and General Self-Efficacy Scale (GSES) from October to December 2017. Single factor analysis, Pearson correlation and multiple linear regression were used to analyze the correlation between core competence and self-efficacy of geriatric clinical nurses and the influencing factors. Results The score of GNCI was (2.51±0.64), and the score of GSES was (2.60±0.65). Pearson correlation analysis showed that core competence of geriatric nursing was positively correlated with self-efficacy (r=0.472, P＜0.01). There were significant differences in the scores of GNCI of geriatric clinical front-line nurses with different ages, working years, educational background, professional title, marriage, personnel nature, number of night shifts on duty, whether they were teaching, professional interests, whether accept advanced study in superior hospitals and family support (P＜ 0.05). There were significant differences in the scores of GSES among clinical front-line geriatric nurses with different ages, marriages, number of night shifts on duty, whether they were teaching, professional interests and family support (P＜0.05). Multivariate linear regression analysis showed that professional interest was the influencing factor of core competence of geriatric nursing (P＜0.05). Conclusions The core competence of clinical front-line nurses in Geriatrics Department is low, and their self-efficacy is moderate. There is a positive correlation between them. As a nursing administrator in Geriatrics Department, it is significant to strengthen the training of clinical front-line nurses, constantly improve the training curriculum and career planning, and improve the core competence and self-efficacy of geriatric nursing.

Objective To explore and analyze the social support and family burden in patients with chronic hepatitis B (CHB).Methods From January to December 2016, a total of 272 patients with CHB and 272 primary caregivers in a Class Ⅲ Grade A hospital in Nantong were selected by convenience sampling method, and investigated by social support rating scale (SSRS) and family burden scale of disease (FBS). The results were statistically analyzed by SPSS 17.0 software, and the correlation between social support and family burden was analyzed by Pearson correlation analysis.Results The score of social support was (44.99±7.21) and family burden was (16.59±11.03). Pearson correlation analyzsis showed a negative correlation between social support and family burden (r=-0.176,P=0.004). The differences of social support were statistically significant among CHB patients with different marital status, monthly income and medical payment method (t/F=5.499, 11.490, 23.140;P<0.05). The differences of family burden were statistically significant among CHB patients' caregivers with different gender, occupation, education level, relation with patients and time caregiving (t/F= 2.412, 2.180, 7.990, 2.714, 6.372;P<0.05).Conclusions Further interventions should be implemented to improve social support and reduce disease burden for patients with CHB.

Objective To explore the efficacy of group intervention on the medication compliance, the degree of family care, negative emotion in patients with chronic hepatitis B. Methods From July to August 2016, a total of 80 patients with chronic hepatitis B who volunteered to take part in this research were selected and divided into two groups by random table method, with 40 cases in each group. The experimental group received group intervention, while the control group were given routine intervention. The medication compliance, the degree of family care and the negative emotion of the two groups were compared before and after intervention. Results After 6 months' intervention, the medication compliance of the experimental group was (0.67±0.85), and that of the control group was (1.20±1.34). The difference was statistically significant (t=2.097, P< 0.05). Patients with good family function in the experimental group increased from 65.00% to 87.50%. The scores of SAS and SDS were (42.92±9.04) and (45.91±8.89), which were both lower than those of the control group, with statistical significance (t=2.231, 2.226;P< 0.05). Conclusions Group intervention could help increase medication compliance and family care degree, at the same time decrease negative emotion in patients with chronic hepatitis B.

Objective To explore the effects of peer support education on the compliance and quality of life in patients with liver cirrhosis complicated with upper gastrointestinal bleeding.Methods A total of 60 patients with liver cirrhosis complicated with upper gastrointestinal bleeding during January 2015 and December 2016 were selected in this study using convenient sampling. Patients who treated from January to December 2015 were included in the control group (n=30) and those treated from January to December 2016 were included in the observation group (n=30). Patients in the control group adopted routine nursing. Based on the routine nursing care, the observation group received additional peer support education. The medication adherence questionnaire and the MOS Item Short Form Health Survey (SF-36) were used to compare the compliance and quality of life before and after the intervention within two groups.Results After six months of intervention, the intervention group had significantly better compliance compared with the control group [(1.23±0.76) vs (2.23±1.45);t=3.144,P<0.05]. The scores of SF-36 (except health dimension) were significantly higher in the observation group compared with the control group (P<0.05).Conclusions Peer support education may be helpful in improving the medication compliance and quality of life in patients with liver cirrhosis complicated with upper gastrointestinal bleeding.