Objective To record and analyze the infrared spectra of rubber substrate and film in common pharmaceutical packaging materials,and to provide technical support and reference for the infrared spectra identification of rubber pharmaceutical packaging materials in the national standards system.Methods The infrared spectra of the rubber substrate and film were recorded by the Fourier transform infrared(FT-IR)-attenuated total reflection(ATR)method.The main absorption peaks in the spectra were assigned,the vibration forms were analyzed,and the influencing factors of infrared spectra were discussed.Results The infrared spectra of silicone rubber,polyisoprene rubber,halogenated butyl rubber,ethylene-tetrafluoroethylene copolymer(ETFE),tetrafluoroethylene-hexafluoropropylene copolymer(FEP)and p-xylene were analyzed.Conclusion The production formula of rubber pharmaceutical packaging materials is complex and diverse.Factors such as test methods,rubber product production formula,ATR crystal materials,and silicone oil will affect the results of infrared spectra.

Objective To investigate the differential expression of miR-124-3p in peripheral blood and clinical sig-nificance of patients with β-thalassemia.Methods Peripheral blood samples were collected from 33 patients with β-thalassemia and 30 healthy controls in Ningde Municipal Hospital Affiliated to Ningde Normal University from June 2021 to August 2022.The expression level of miR-124-3p was detected.Luciferase reporter gene was used to verify the interaction between miR-124-3p and ERF 3'UTR.The correlation between differential expression of miR-124-3p and β-thalassemia was analyzed and the clinical diagnostic value of miR-124-3p for β-thalassemia was eval-uated.Results Compared with healthy control individuals,the expression of miR-124-3p was significantly up-reg-ulated in patients with β-thalassemia(P＜0.001).The genotype of miR-124-3p high expression group was 84.2%β0(16/19),the genotype of low expression group was 55.6%β+(10/18).ROC curve analysis showed that miR-124-3p had predictive efficacy for β-thalassemia(AUC:0.842).Luciferase reporter gene analysis showed that ERF gene was the regulatory target of miR-124-3p.Conclusions The differential expression of miR-124-3p in patients with β-thalassemia is closely related to the genotype and clinical severity of thalassemia,and ERF is negatively reg-ulated by miR-124-3p.miR-124-3p may be an effective diagnostic biomarker for β-thalassemia.

[Objective] The aim of this study was to investigate the protective role of programmed cell death ligand-1 (PD-L1) in a mouse model of spinal cord injury (SCI) by regulating T cell immunity and the PI3K/Akt/mTOR signaling pathway. [Methods] C57BL/6 mice used to establish SCI models were divided into the sham operation group (Sham), SCI group, SCI+ PD-L1 antibody group (SCI+ Ab), and SCI+ PD-L1 protein group (SCI+ PRO). c57BL/6 mice and PD-L1 knockout mice were used for SCI mapping, and they were divided into the sham operation group (Sham WT), PD-L1 knockout sham operation group (Sham PD-L1 KO), SCI model group (SCI WT), and PD-L1 knockout SCI model group (SCI PD-L1 KO). Western blotting and qRT-PCR were applied to detect the expression of PD-L1 in spinal cord tissues at different time points after SCI; mouse motor function was assessed by the Basso Mouse Motor Scale (BMS); changes in the levels of inflammatory factors and T-cell subpopulations after SCI were analyzed using qRT-PCR and flow cytometry; and Western blotting was used to detect changes in the PI3K/Akt/mTOR signaling pathway activation. [Results] PD-L1 expression was upregulated in spinal cord tissues of mice subjected to SCI palliation, peaking on day 7. Compared with the SCI PD-L1 KO group, mice in the SCI WT group had significantly higher BMS scores at 7, 14, and 28 days after SCI (P<0.05), and the levels of inflammatory factors IL-1α, IL-2, IFN-γ and TNF-α were significantly lower on day 7 after palpation (P<0.05). Compared with the SCI+ PBS group, mice in the SCI+ PRO group had significantly higher Foxp3 levels and significantly lower Th1 and Th17 levels. Foxp3 levels were significantly higher, but Th1 and Th17 cell levels were significantly lower, and Th2 and Treg cell levels were significantly higher (P<0.05). The phosphorylation of the PI3K/Akt/mTOR signaling pathway was significantly higher in the SCI WT group mice than the SCI PD-L1 KO group ones (P<0.05). In contrast, the phosphorylation of PI3K/Akt/mTOR signaling pathway was significantly lower in the SCI+ PRO group than in the SCI+ PBS group and the SCI+ Ab group (P<0.05). [Conclusion] PD-L1 plays a neuroprotective role by regulating the balance of Th1, Th17, Th2 and Treg cells and inhibiting the PI3K/Akt/mTOR signaling pathway, thereby reducing the inflammatory response after SCI. PD-L1 is expected to be a new target for the treatment of SCI.

OBJECTIVETo detect potential mutation of EXT1 gene in a pedigree affected with multiple osteochondroma and explore its pathogenic mechanism.

METHODSThe coding regions and their flanking sequences of the EXT1/EXT2 genes were subjected to PCR amplification and Sanger sequencing. Suspected mutations were verified by excluding possible single nucleotide polymorphisms and bioinformatics analysis. Transcripts of the EXT1 gene in the proband were analyzed by TA clone-sequencing, with its abundance compared with that of healthy controls.

RESULTSDNA sequencing has identified in the proband a novel heterozygous point mutation (c.1164+1G to A) at the 5'splice sites of intron 3 of the EXT1 gene. The same mutation was not found in the healthy controls. Bioinformatics analysis indicated that the mutation is highly conserved and can lead to skipping of exon 3 or aberrant splicing. TA clone-sequencing indicated that the numbers of transcripts with skipping of exon 3 has significantly increased in the proband (< 0.05) compared with the controls.

CONCLUSIONThe c.1164+1G to A mutation has resulted in skipping of exon 3 in a proportion of EXT1 gene transcripts. As the result, the number of transcripts with tumor suppressing function is relatively reduced and has ultimately led to the tumors.

Adult ; Base Sequence ; Child ; Exostoses, Multiple Hereditary ; genetics ; Female ; Humans ; Male ; Molecular Sequence Data ; N-Acetylglucosaminyltransferases ; genetics ; Point Mutation ; RNA Splice Sites ; RNA Splicing

Objective To investigate the changes and correlation of mitral valve coaptation length index CLI and coaptation area index CAI after mitral valvuloplasty MVP Methods A total of 30 subjects undergoing MVP for mitral regurgitation MR were studied Coaptation length CL CLI coaptation area CA and CAI were determined before and after surgery by 2-dimensional transoesophageal echocardiography 2D-TEE and 3-dimensional transoesophageal echocardiography 3D-TEE Results Compared with preoperative measurements CL CLI CA and CAI were significantly increased in postoperative studies CL 4 7±0 7 mm vs 9 4± 1 1 mm CLI 9 1 ±3 3 vs 38 5 ±4 1 CA 148 9 ± 65 3 mm 2 vs 371 9 ± 144 3 mm 2 CAI 9 3 ±3 1 vs 35 9 ± 7 5 all P < 0 05 CLI was significantly correlated with CAI both preoperatively r = 0 770 P < 0 01 and postoperatively r = 0 771 P <0 01 Furthermore CLI and CAI were significantly negative correlated with the degree of MR r =-0 897 P <0 01 r =-0 886 P <0 01 Conclusions Coaptation variables increased significantly in subjects after MVP CLI by 2D-TEE was related to CAI by 3D-TEE and both were useful for the assessment of mitral valve coaptation But CLI by 2D-TEE was more simple and feasible in clinic.

Objective:Some medical liquid needs to be heated to the human body temperature, so the constant temperature case is a necessary equipment. But the common constant temperature case is often bulky, inconvenient application and the price is very high, so this paper is mainly to develop a convenient and cheap thermostat device for medical liquid heating.Methods:The system used single-chip microcomputer for temperature control. The temperature sensor collected and displayed the temperature of the box when the temperature was set up. Then the heating module started when the temperature was lower than the set point, and stopped heating when reaching to the set point.Results: The temperature control effect of constant temperature case tested by calibrated mercury thermometer, the temperature of constant temperature case was not significantly different from that of mercury thermometer.Conclusion: The portable constant temperature case for medical liquid have the characteristics of precise temperature control, high temperature resolution, and small temperature fluctuations. It can meet the clinical needs and be worth to popularizing in clinical applications.

To introducethe high frequency electro-surgical unit safety operation, reduce the pain of the patients, to maximize the effect of high frequency electro-surgical unit.

ObjectiveThe purpose of this study is to discuss the diagnostic accuracy of nornalized liver ADC using the spleen and renal cortex as reference organs for the diagnosis of liver fibrosis.Methods Forty three patients with liver disease (chronic liver disease group) at compensated stage and 10 healthy volunteers (control group) were retrospectively assessed with diffusion-weighted imaging at a 3.0 T MR unit.Liver ADC,spleen ADC,renal ADC and normalized ADC (defined as the ratio of liver ADC to spleen ADC or renal cortex ADC,S-ADC and R-ADC for short) were measured in patients stratified by fibrosis stage.Spearman analysis was used to see the correlation between fibrosis stages and ADC,one-way ANOVA was used to compare the ADCs in different fibrosis stages.Logistic regression analysis was used to determine the performance of ADC for prediction of liver fibrosis,and show the area under the curve ( AUC),sensitivity and specificity choosing the optimal cutoff value that maximized the Youden index.ResultsTen volunteers belonged to SO stage.From SO to S4 stage,there were 2,5,9,12 and 15 patients,correspondingly,liver ADCwere (1.37±0.13) ×10-3,(1.33±0.16) ×10 -3,(1.17±0.16) ×10-3,(1.23±0.14) ×10-3and ( 1.12 ±0.11 ) × 10-3mm2/s,S-ADC were 1.86 ±0.18,1.68 ±0.12,1.34 ±0.14,1.48 ±0.15 and 1.34±0.10,R-ADC were 0.71 ±0.08,0.68 ±0.12,0.61 ±0.09,0.64 ±0.11 and 0.60 ±0.08respectively,and the differences among them were significant ( F =6.48,18.70 and 3.04,P ＜0.05 ).The correlation between fibrosis stage and S-ADC was stronger than between fibrosis stage and liver ADC,R-ADC (r =- 0.71,- 0.51,- 0.41 ;P ＜ 0.01 ).S-ADC was superior to liver ADC and R-ADC for detection of S2,S3 and S4 fibrosis stage (Youden index:0.91,0.58,and 0.59).ConclusionSpleen normalized liver ADC improves diagnostic accuracy for detection of liver fibrosis than liver ADC and renal normalized liver ADC.

Objective To explore the manifestations of chest multi-slice spiral CT in patients with initial infection of swine-origin influenza A (H1N1) virus (S-OIV). Methods The chest multi-slices spirals CT images of 19 firstly diagnosed patients with swine-origin influenza A (H1N1) in our institution were retrospectively studied. CT manifestations were evaluated by three experienced radiologists. Location, appearance of lung abnormalities, abnormal distribution, pleural effusion and others (pericadiaum, lymphadenopathy and pleural thickening) were observed and quantitatively analyzed. The correlation of ground-glass and consolidation CT scores with the fever time was studied. Results The abnormal CT findings were observed bilaterally in 18 of 19 subjects including ground-glass (n= 3), consolidation (n=3 ), consolidation accompanied with ground-glass (n=12). Most of these lesions were distributed diffusively (n=14) while the others located in the middle and low lobes (n= 4). Unilateral (n=3) or bilateral (n=2) pleural effusion were observed. Lymphadenopathy (n=2), effusion of pericadium (n=1), pleural thickening (n=1) and cardiac enlargement (n=2) were also found in patients with H1N1. CT scores of ground-glass were 4. 25(n=2),3.75 (n=1),2.25(n=1),1.75(n=1),1.00(n=6),0.75(n=2), 0.50(n=2),0(n=4).CT scores of consolidation were4.25(n=1),4.00(n=1),3.75 (n=1), 2.75(n=1),1.25(n=3),1.00(n=2),0.75(n=2),0.50(n=1),0.25(n=3),0(n=4). CT scores of ground-glass were significantly correlated with the fever time (r= 0.776, P < 0.01), CT scores of consolidation had no correlation with the fever time(r=0.322,P > 0.01). Conclusions The most common CT findings in patients with S-OIV infection are diffuse distribution of bilateral ground-glass opacities with or without associated focal or multifocal areas of consolidation. The increasing of ground-glass's range could be the marker of progression of H1N1 pulmonary infection at initial stage.

Objective The study was to evaluate DWI for quantifying liver fibrosis. Methods A total of 12 volunteers, 47 patients who had chronic HBV or HCV hepatitis and underwent liver biopsy [Scheuer score for fibrosis(S) and inflammation(G)] were enrolled in this study. They were scanned using a 1.5 T MR unit with b value of 0,250,500,750, 1000 s/mm~2. ADCs at b_(250-1000) and b_(500-1000) were the average ADCs of b=250, 500, 750, 1000 s/mm~2 and b=500, 750, 1000 s/mm~2. The studied the correlation between Scbeuer scores and ADC values, and conducted Mann-Whitney U test and Logistic regression to evaluate ADC for prediction of fibrosis scores. Results The average ADCs were (1.41± 0.11),(1.37±0.09), (1.27±0.05), (1.26±0.04), (1.22±0.06) mm~2/s respectively from SO to S4, stage at b=750 s/mm~2 (F=18.31, P<0.01). With the increase of fibrosis score, the average ADC decreased gradually, the two were better negatively correlated at b_(250-1000)(r=-0.727, P<0.01) than other b values. Using b_(750) and the two combined b values, the found significantly lower ADCs in S2 or greater versus S1 or less and in S3 or greater versus S2 or less fibrosis (P<0.01). The best predictor for S2 or greater was b_(750) with the largest AUC of 0.909, sensitivity of 85.7%, and specificity of 100.0% (ADC ≤1.35×10~(-3) mm~2/s). The best predictor for S3 or greater was b_(250-1000) with the largest AUC of 0.864, sensitivity of 69.6%, and specificity of 95.8% (ADC≤1.53×10~(-3) mm~2/s). Conclusion DWI can be a good predictor for scoring liver fibrosis for S2 or S3 stage above, while b_(750) and the combined b values are suitable for evaluation.