Evoked Potentials ; Social Isolation ; Attention ; Humans

Objective:To investigate the correlation between the changes of oral bacterial flora during head and neck radiotherapy and radiation-induced oral mucositis (ROM).Methods:The oral bacterial samples of patients with nasopharyngeal carcinoma and accompanying family members were obtained before and at the end of radiotherapy and subjected to high-throughput sequencing. C57BL/6 mice were used to establish the ROM models. On the 9 th day after radiotherapy, oral bacterial samples were collected in the radiotherapy group and the negative control group. On the 3 rd, 5 th, 7 th, and 9 th days post-radiotherapy, the tongue tissues were obtained from another batch of mice in the negative control and radiotherapy groups. Inflammatory factors were detected with PCR and HE staining was performed. Results:The oral bacterial diversity of patients after radiotherapy significantly differed from that of patients before radiotherapy and their accompanying family members before and after radiotherapy in Observed species, Chao1, Simpson index (all P<0.05). There was a significant difference in Shannon index between the severe and mild ROM patients ( P=0.036). LEfSe analysis showed that patients with severe ROM had higher levels of g_ Streptococcus and f_ Streptococcus, and lower levels of f_ Familyxl, g_ Gemini and o_ Bacillus. The Simpson index and PCoA results in the oral bacterial samples significantly differed between the negative control and radiotherapy groups (all P<0.05). Conclusions:Radiotherapy can disrupt the balance of bacterial flora. The dysregulated oral bacterial flora is closely associated with the aggravation of ROM.

Objective:To evaluate the effectiveness of interventional treatment for neonatal critical pulmonary stenosis(NCPS).Methods:Clinical data of 12 neonates with NCPS who received percutaneous balloon pulmonary valvuloplasty (PBPV) from January 2016 to December 2019 in Department of Cardiology, Shenzhen Children′s Hospital were summarized and analyzed.The collected data included transthoracic echocardiography (TTE), percutaneous oxygen saturation (SPO 2), relevant data on interventional surgery, and follow-up results. Results:All 12 neonates with NCPS received PBPV successfully.The postoperative pressure difference between the right ventricle and the pulmonary artery ranged from 8 to 35 mmHg[(20±7) mmHg, 1 mmHg=0.133 kPa]. The postoperative SPO 2 ranged from 74%-100%[(93.0±5.9)%]. Three neonates with NCPS received Blalock-Taussig (B-T) shunt.One neonate with NCPS developed supraventricular tachycardia during the operation.There was no death for these 12 neonates with NCPS. Conclusions:Interventional treatment of neonates with NCPS could achieve a better effect and be employed as the first treatment option.Some neonates with NCPS would require cardiac B-T shunt or patent ductus arteriosus stent implantation.

Objective To design and apply the nursing quality management system of our hospital in order to strengthen nursing informationization construction, and improve management efficiency of nursing quality. Methods Based on the original design of nursing management system, the quality management system which take nursing as the main part was researched and developed by us and applied to the clinical. Our nursing management department combined PDCA cycle with our nursing quality management, and developed nursing unit quality evaluation criteria, each ward were checked by evaluating group of nursing quality management, the analysis and corrective measures should be asked to put forward. Results From July to December in 2016, nursing accidents, pressure ulcer, tumbling, pipe slippage, medication, errors and all adverse events incidence compared with 2015 year dropped 60.00％(0.03/0.05), 43.33％ (0.13/0.30), 50.00％ (0.04/0.08), 40.00％ (0.04/0.10), 33.33％ (0.01/0.03),43.85％(0.25/0.57), and all adverse events in 2016 compared to the period of 2015 had statistically difference (χ2=7.266, P<0.05), and the problem rectification rate was significantly higher than before(χ2=-3.212--2.637,P<0.05). Conclusion The construction and application of nursing quality management system realized the informationization and resource sharing of nursing quality management, it will help the quality of management to upgrade continuously and improve the efficiency of nursing quality management.

Objective? To investigate the feasibility of Modified Early Warning Score (MEWS) in evaluating the condition of emergency patients and as a basis for emergency triage. Methods? A total of 1 643 patients in the Rescue Room of Emergency Department of Suzhou Integrated Traditional Chinese and Western Medicine Hospital were selected by convenience sampling method from 11th April 2016 to 2nd August 2017. All patients were followed up, and MEWS of patients with different directions were compared. The area under the curve of MEWS and Yoden index were calculated by ROC curve to predict the mortality of patients. Comparing the MEWS of patients in general wards and ICU, the area under the curve of the MEWS was predicted by ROC curve, and the critical value was selected by Yoden index. Results? There were 475 cases discharged, 326 cases observed in hospital, 768 hospitalized (101 cases were admitted to ICU, 667 cases were admitted to general wards) and 74 cases died. There was significant difference in MEWS among the four groups (H=236.45, P＜0.001). When the best critical point was 3.5 of MEWS, the area under ROC curve for predicting death was 0.910, 95%CI (0.87-0.95), and Yoden index was 0.72. There was significant difference in MEWS between patients admitted to general wards and ICU (Z=-9.005, P＜ 0.001). When picking 2.5 of MEWS as the best critical point, the area under the ROC curve for predicting ICU admission was 0.786, 95%CI (0.73-0.85), and the Youden index was 0.50. Conclusions? The MEWS has clinical significance in predicting the death of emergency patients and assessing the severity of patients. It provides an objective basis for the correct shunt of emergency patients and the correct identification of potentially critically ill patients.

The measurement of portal vein pressure (PVP) is important for the evaluation of therapeutic efficacy and prognosis in patients with liver cirrhosis.Aims: To investigate a non-invasive method for evaluating PVP in model of liver cirrhosis in rats.Methods: Liver cirrhosis model in rats was induced by intraperitoneal injection with thioacetamide.Magnetic resonance imaging with TOF sequence was used to measure portal vein diameter (PVD).PVP was detected directly by transvenous catheterization of portal vein.Body weight, liver weight, spleen weight, liver volume and spleen volume were determined.The hydroxyproline content in liver was determined by alkaline hydrolysis assay, proportion of collagen area in liver was detected by Sirius red staining.Results: Liver cirrhosis model in rats was successfully established after intraperitoneal injection for 20 weeks.Compared with control group, mean PVP, liver weight, liver volume, spleen weight, PVD, liver volume/body weight (LV/BW) ratio, spleen volume/body weight (SV/BW) ratio, hydroxyproline content and proportion of collagen area were significantly increased in model group (P<0.05), and body weight was significantly decreased (P<0.001).PVP was positively correlated with LV/BW ratio and proportion of collagen area (P<0.05).Conclusions: LV/BW and proportion of collagen area can indirectly reflect the PVP, and may provide a non-invasive approach for evaluation of portal hypertension.

Objective To investigate whether FTO gene SNP polymorphisms were associated with metabolic traits in Chinese children. Methods A total of 595 children aged between 6 and 17 years were enrolled. The five common SNPs in the FTO gene(rs1421085,rs17817449,rs8050136,rs3751812 and rs9939609)were genotyped. Metabolic compo-nents and insulin were measured. Results (1)The obese patients showed the components of the metabolic traits were significant difference compared to control group.(2)The allele frequency and the genotype distribution of the five SNPs were significant differences between two groups. Minor allele carriers of the five SNPs were associated with increased risks of childhood obesity compared with the wild-type genotype. (3)After adjustment of age, sex and BMI-z score,all of the five SNPs showed a trend towards higher SBP. In addition, rs3751812 and rs8050136 showed significant as-sociation with LDL-C and HOMA-IR levels. Conclusion The five common SNPs in the FTO gene contributes to the e-tiology of obesity and hypertension. The FTO gene polymorphisms (rs3751812 and rs8050136)are found to be associ-ated with increased insulin resistance and plasma LDL levels.

OBJECTIVETo compare and evaluate clinical applications of two definitions of metabolic syndrome in children and adolescents, which was developed by Pediatric Academy of Chinese Medical Association in 2012 (Chinese definition) and by International Diabetes Federation in 2007 (IDF definition), respectively.

METHODS593 obese children and adolescents aged 10 ≊16 y from July 2006 to December 2012 were enrolled in the study. The diagnostic concordance of two definitions for metabolic syndrome and individual components was estimated, and their sensitivity and specificity for detecting insulin resistance and early macrovascular complications were compared.

RESULTSThe concordance between two definitions for diagnosing metabolic syndrome was good (kappa=0.626); as for detecting the individual components, the Kappa concordance index were 1.000, 0.803, 0.780, 0.734 and 0.594 for hypertriglyceridemia, hyperglycemia, cholesterol abnormality and hypertension, respectively. The incidence of insulin resistance and early macrovascular complications, detected by the two definitions, were both increased with increasing number of abnormal components. The sensitivity and specificity for detecting insulin resistance in children with metabolic syndrome were 54.5% and 65.7% by Chinese definition, and 36.1% and 83.1% by IDF definition; while the sensitivity and specificity for detecting early macrovascular complications were 58.3% and 55.8% by Chinese definition, and 37.3% and 70.8% by IDF definition. After adjusting for age and sex, compared to the obese children and adolescents without metabolic syndrome, the odds ratios of insulin resistance and early macrovascular complications were 2.166 (P<0.001) and 1.771(P=0.008) for children with metabolic syndrome diagnosed by Chinese definition, and the odds ratio of insulin resistance and early macrovascular complications were 2.618 (P<0.001) and 1.357 (P=0.190) by IDF definition.

CONCLUSIONThe concordance between Chinese and IDF definitions for diagnosing metabolic syndrome in Chinese obese children and adolescents is good. Compared to IDF definition, Chinese definition is more sensitive for hypertension, hyperglycemia and hypercholesterolemia, thus it can more effectively detect insulin resistance and early macrovascular complication.

Adolescent ; Child ; Female ; Humans ; Insulin Resistance ; Male ; Metabolic Syndrome ; classification ; complications ; diagnosis ; Obesity ; complications ; Sensitivity and Specificity

To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect melamine residues in dairy products and feed sample. Colloidal gold particles labeled with purified monoclonal antibody against anti-melamine were used as the detector reagent. The MEL-OVA (the conjugate of melamine and ovalbumin) and goat anti-mouse melamine IgG were blotted on the test and control regions of nitrocellulose membrane. The strip was then assembled with sample pad, absorbing pad, and dorsal shield. The limit of detection (LOD) is 50 microg/L. The test trip was applied to detect melamine in milk, milk powder, and animal feeds, with detection limits of 100 microg/L for milk, 100 ng/g for milk powder, 200 ng/g for feeds. Compared to LC-MS/MS, the ICA could be used to screen a large number of dairy products and feed samples for melamine residue.
Animals ; Antibodies, Monoclonal ; chemistry ; Cattle ; Dairy Products ; analysis ; Food Contamination ; analysis ; Gold Colloid ; chemistry ; Immunochromatography ; methods ; Milk ; chemistry ; Reagent Strips ; chemical synthesis ; chemistry ; Sensitivity and Specificity ; Triazines ; analysis

Objective To assess the value of quantitative color Doppler sonography in patients with systemic lupus erythematosus (SLE). Methods The right renal in 21 patients with SLE and 22 normal subjects were examined with Philips IU22 ultrasonic diagnostic device. Resistence index (RI) of interlobar artery and interlobular artery were measured with color Doppler sonography, vascularity index (VI), flow index (FI) and vascularization flow index (VFI) which reflecting the renal cortical blood flow were calculated with quantitated analysis software. All the parameters between two groups were compared and analyzed. Results The color signals of renal cortical blood flow in patients with SLE were inferior to those of the normal group, especially in the area near the edge of renal. No statistical difference of RI was found between the two groups (P>0.05), while VI, FI and VFI in SLE group were significantly lower than those of the normal group (VI, VFI, P<0.01, FI, P=0.01). Conclusion Color Doppler sonography combined with blood flow quantitative technique provide a helpful, sensitive and convenient tool to detecte the renal changes in SLE patients.