Objective To investigate the effects of transforming growth factor-?1 (TGF-? 1) on the synthesis of FN and ? 5? 1 integrin b y cultured human fibroblast isolated from hypertrophic scars. Methods The influence of TGF-? 1 at concentration of 0, 5, 10, 25, 50 and 100 ?g/L on the synthesis of FN in dosage was observed using ELISA, an d influence of TGF-? 1 on the synthesis of ? 5? 1 integrin was assessed b y immunohistocytochemistry and image analysis. Results TG F-? 1 could stimulate the human fibroblasts isolated from hypertrophic scars to synthesize FN and? 5? 1 integrin with dose-dependent increase at concent ration of 10～50 ?g/L and the effects TGF-? 1 on the synthesis was the stron g est at concentration of 100 ?g/L. Conclusions TGF-? 1 may exert its function on the formation of hypertrophic scars by stimulating th e synthesis of FN and ? 5? 1 integrin.