1.Health benefit utilization and out-of-pocket expenses in outpatient care and hospitalizations: Baseline surveys of three primary care sites in the Philippines
Leonila F. Dans ; Jose Rafael A. Marfori ; Regine Ynez H. De Mesa ; Cara Lois T. Galingana ; Noleen Marie C. Fabian ; Mia P. Rey ; Josephine T. Sanchez ; Jesusa T. Catabui ; Nannette B. Sundiang ; Ramon Pedro P. Paterno ; Edna Estifania A. Co ; Carol Stephanie C. Tan-Lim ; Antonio Miguel L. Dans
Acta Medica Philippina 2024;58(Early Access 2024):1-8
		                        		
		                        			Background:
		                        			The Philippine Primary Care Studies (PPCS) is a network of pilot studies that developed, implemented, and tested strategies to strengthen primary care in the country. These pilot studies were implemented in an urban, rural, and remote setting. The aim is to use the findings to guide the policies of the national health insurance program (PhilHealth), the main payor for individualized healthcare services in the country.
		                        		
		                        			Objective:
		                        			The objective of this report is to compare baseline outpatient benefit utilization, hospitalization, and health spending, including out-of-pocket (OOP) expenses, in three health settings (urban, rural, and remote). These findings were used to contextualize strategies to strengthen primary care in these three settings.
		                        		
		                        			Methods:
		                        			Cross-sectional surveys were carried out using an interviewer-assisted questionnaire on a random sample of families in the urban site, and a stratified random sample of households in the rural and remote sites. The questionnaire asked for out-patient and hospitalization utilization and spending, including the OOP expenses.
		                        		
		                        			Results:
		                        			A total of 787 families/households were sampled across the three sites. For outpatient benefits, utilization was low in all sites. The remote site had the lowest utilization at only 15%. Unexpectedly, the average annual OOP expenses for outpatient consults in the remote site was PhP 571.92/per capita. This is 40% higher than expenses shouldered by families in the rural area, but similar with the urban site.
 For hospital benefits, utilization was lowest in the remote site (55.7%) compared to 75.0% and 78.1% for the urban and rural sites, respectively. OOP expenses per year were highest in the remote site at PhP 2204.44 per capita, probably because of delay in access to healthcare and consequently more severe conditions. Surprisingly, annual expenses per year for families in the rural sites (PhP 672.03 per capita) were less than half of what families in the urban sites spent (PhP 1783.38 per capita).
		                        		
		                        			Conclusions
		                        			Compared to families in the urban site and households in the rural sites, households in remote areas have higher disease rates and consequently, increased need for outpatient and inpatient health services. When they do get sick, access to care is more difficult. This leads to lower rates of benefit utilization and higher out-of-pocket expenses. Thus, provision of “equal” benefits can inadvertently lead to “inequitable” healthcare, pushing disadvantaged populations into a greater disadvantage. These results imply that health benefits need to be allocated according to need. Families in poorer and more remote areas may require greater subsidies.
		                        		
		                        		
		                        		
		                        	
2.Health benefit utilization and out-of-pocket expenses in outpatient care and hospitalizations: Baseline surveys of three primary care sites in the Philippines.
Leonila F. Dans ; Jose Rafael A. Marfori ; Regine Ynez H. De Mesa ; Cara Lois T. Galingana ; Noleen Marie C. Fabian ; Mia P. Rey ; Josephine T. Sanchez ; Jesusa T. Catabui ; Nannette B. Sundiang ; Ramon Pedro P. Paterno ; Edna Estifania A. Co ; Carol Stephanie C. Tan-Lim ; Antonio Miguel L. Dans
Acta Medica Philippina 2024;58(16):133-140
BACKGROUND
The Philippine Primary Care Studies (PPCS) is a network of pilot studies that developed, implemented, and tested strategies to strengthen primary care in the country. These pilot studies were implemented in an urban, rural, and remote setting. The aim is to use the findings to guide the policies of the national health insurance program (PhilHealth), the main payor for individualized healthcare services in the country.
OBJECTIVEThe objective of this report is to compare baseline outpatient benefit utilization, hospitalization, and health spending, including out-of-pocket (OOP) expenses, in three health settings (urban, rural, and remote). These findings were used to contextualize strategies to strengthen primary care in these three settings.
METHODSCross-sectional surveys were carried out using an interviewer-assisted questionnaire on a random sample of families in the urban site, and a stratified random sample of households in the rural and remote sites. The questionnaire asked for out-patient and hospitalization utilization and spending, including the OOP expenses.
RESULTSA total of 787 families/households were sampled across the three sites. For outpatient benefits, utilization was low in all sites. The remote site had the lowest utilization at only 15%. Unexpectedly, the average annual OOP expenses for outpatient consults in the remote site was PhP 571.92/per capita. This is 40% higher than expenses shouldered by families in the rural area, but similar with the urban site. For hospital benefits, utilization was lowest in the remote site (55.7%) compared to 75.0% and 78.1% for the urban and rural sites, respectively. OOP expenses per year were highest in the remote site at PhP 2204.44 per capita, probably because of delay in access to healthcare and consequently more severe conditions. Surprisingly, annual expenses per year for families in the rural sites (PhP 672.03 per capita) were less than half of what families in the urban sites spent (PhP 1783.38 per capita).
CONCLUSIONSCompared to families in the urban site and households in the rural sites, households in remote areas have higher disease rates and consequently, increased need for outpatient and inpatient health services. When they do get sick, access to care is more difficult. This leads to lower rates of benefit utilization and higher out-of-pocket expenses. Thus, provision of “equal” benefits can inadvertently lead to “inequitable” healthcare, pushing disadvantaged populations into a greater disadvantage. These results imply that health benefits need to be allocated according to need. Families in poorer and more remote areas may require greater subsidies.
Primary Health Care ; Insurance, Health
3.Turnaround time of consults in a primary care system in rural Philippines: A descriptive retrospective cohort study
April Faye P. Barbadillo ; Leonila F. Dans ; Carol Stephanie C. Tan-Lim ; Cara Lois T. Galingana ; Josephine T. Sanchez ; Maria Rhodora N. Aquino ; Arianna Maever L. Amit ; Regine Ynez H. De Mesa ; Mia P. Rey ; Janelle Micaela S. Panganiban ; Karl Engelene E. Poblete ; Nanette B. Sundiang ; Antonio L. Dans
Acta Medica Philippina 2024;58(18):20-26
		                        		
		                        			Background:
		                        			Turnaround time is an integral component of primary healthcare and is a key performance indicator of healthcare delivery. It is defined as the time patients spend during a healthcare facility visit. In this study, turnaround time is defined as the time elapsed from registration to the end of consultation.
		                        		
		                        			Objectives:
		                        			This study aimed to determine the turnaround time of consults in the primary care system in a rural site in the Philippines, and compare turnaround time during the pre-pandemic and COVID-19 pandemic periods.
		                        		
		                        			Methods:
		                        			This is a retrospective cohort study of patients seen at the primary care facility under the Philippine Primary Care Studies (PPCS) rural site from April 2019 to March 2021. Patients included in this study were chosen through random sampling. Electronic medical records (EMR) of these patients were reviewed. Turnaround time was computed electronically from time of registration to end of consultation. Descriptive statistics was used to summarize data and report turnaround time. The turnaround time before and during the pandemic was compared using an independent sample t-test (if normally distributed) or Mann Whitney U test (if not normally distributed). A p-value of <0.05 was considered statistically significant.
		                        		
		                        			Results:
		                        			A random sample of 342 patients out of the total 45,501 patient consults seen at the rural primary healthcare facility from April 2019 to March 2021 were included in this study. The median turnaround time was 29.0 minutes (interquartile range [IQR] 68.3), with range of 0.9 to 437.2 minutes. During the pre-pandemic period, the median turnaround time of consults is 29.3 minutes (IQR 70.4) which is 1.8 minutes longer than the pandemic period which showed median turnaround time of 27.5 minutes (IQR 72.7). The difference between the two time periods was not statistically significant (P = 0.39).
		                        		
		                        			Conclusion
		                        			The study showed that the median turnaround time of medical consults was 29.0 minutes, which was shorter by 80 minutes compared to other published Philippine studies. The turnaround time did not differ significantly in the pandemic and prepandemic period, despite new policies and systems that were implemented during the pandemic.
		                        		
		                        		
		                        		
		                        			primary care
		                        			;
		                        		
		                        			electronic medical records
		                        			;
		                        		
		                        			pandemic
		                        			;
		                        		
		                        			Philippines
		                        			
		                        		
		                        	
4.2021 Asian Pacific Society of Cardiology Consensus Recommendations on the use of P2Y12 receptor antagonists in the Asia-Pacific Region: Special populations.
W E I C H I E H T A N TAN ; P C H E W CHEW ; L A M T S U I TSUI ; T A N TAN ; D U P L Y A K O V DUPLYAKOV ; H A M M O U D E H HAMMOUDEH ; Bo ZHANG ; Yi LI ; Kai XU ; J O N G ONG ; Doni FIRMAN ; G A M R A GAMRA ; A L M A H M E E D ALMAHMEED ; D A L A L DALAL ; T A N TAN ; S T E G STEG ; N N G U Y E N NGUYEN ; A K O AKO ; A L S U W A I D I SUWAIDI ; C H A N CHAN ; S O B H Y SOBHY ; S H E H A B SHEHAB ; B U D D H A R I BUDDHARI ; Zu Lv WANG ; Y E A N Y I P F O N G FONG ; K A R A D A G KARADAG ; K I M KIM ; B A B E R BABER ; T A N G C H I N CHIN ; Ya Ling HAN
Chinese Journal of Cardiology 2023;51(1):19-31
5.Long term follow-up evaluation of combined surgery for congenital tibial pseudarthrosis in children.
Y X LIU ; G YANG ; X K HU ; Q TAN ; H PAN ; K LIU ; Y Y HUANG ; A YAN ; G H ZHU ; H B MEI
Chinese Journal of Surgery 2023;61(8):675-680
		                        		
		                        			
		                        			<b>Objective:b> To explore the long-term effect of combined surgery for the treatment of congenital tibial pseudarthrosis in children. <b>Methods:b> The clinical data of 44 children with congenital tibial pseudarthrosis who underwent combined surgery (tibial pseudarthrosis tissue resection, intramedullary rod fixation, Ilizarov external fixator fixation, wrapped autologous iliac bone graft) from August 2007 to October 2011 at the Department of Pediatric Orthopedics, Hunan Children's Hospital were collected retrospectively. There were 33 males and 11 females. The age at the time of surgery was (3.7±2.2)years (range:0.6 to 12.4 years), including 25 cases under 3 years old and 19 cases above 3 years old.Among them, 37 cases were complicated with neurofibromatosis type 1.The operation status, postoperative complications and follow-up results were recorded. <b>Results:b> The follow-up time after surgery was (10.9±0.7)years (range:10 to 11 years).Thirty-nine out of 44 patients (88.6%) achieved initial healing of tibial pseudarthrosis, with an average healing time of (4.3±1.1)months (range:3 to 10months).In the last follow-up, 36 cases (81.8%) had unequal tibial length, 20 cases (45.4%) had refractures, 18 cases (40.9%) had ankle valgus, 9 cases (20.4%) had proximal tibial valgus, and 11 cases (25.0%) had high arched feet.Nine cases (20.4%) developed distal tibial epiphyseal plate bridging.17 cases (38.6%) had abnormal tibial mechanical axis.Seven cases (15.9%) developed needle infection, and one case (2.3%) developed tibial osteomyelitis. 21 patients (47.7%) had excessive growth of the affected femur.Five patients (11.3%) had ankle stiffness, and 34 patients (77.2%) had intramedullary rod displacement that was not in the center of the tibial medullary cavity.Among them, 8 cases (18.1%) protruded the tibial bone cortex and underwent intramedullary rod removal.18 children have reached skeletal maturity, while 26 children have not been followed up until skeletal maturity. <b>Conclusion:b> Combined surgery for the treatment of congenital pseudarthrosis of the tibia in children has a high initial healing rate, but complications such as unequal tibia length, refracture, and ankle valgus occur during long-term follow-up, requiring multiple surgical treatments.
		                        		
		                        		
		                        		
		                        			Male
		                        			;
		                        		
		                        			Female
		                        			;
		                        		
		                        			Humans
		                        			;
		                        		
		                        			Child
		                        			;
		                        		
		                        			Child, Preschool
		                        			;
		                        		
		                        			Pseudarthrosis/congenital*
		                        			;
		                        		
		                        			Follow-Up Studies
		                        			;
		                        		
		                        			Retrospective Studies
		                        			;
		                        		
		                        			Tibia/surgery*
		                        			;
		                        		
		                        			Neurofibromatosis 1
		                        			;
		                        		
		                        			Tibial Fractures/surgery*
		                        			
		                        		
		                        	
6.Validation of snort-spit saliva in detecting COVID-19 using RT-PCR and Rapid Antigen Detection Test
Ryner Jose D. Carrillo ; Abigail D. Sarmiento ; Mark Anthony C. Ang ; Michelle H. Diwa ; Cecille C. Dungog ; Daniel I. Tan ; Jan Alexis C. Lacuata ; Jacob Ephraim D. Salud ; Ramon Antonio B. Lopa ; John Mark S. Velasco ; Regina P. Berba ; Charlotte M. Chiong
Acta Medica Philippina 2021;55(2):211-215
		                        		
		                        			
		                        			Objective. To determine the diagnostic accuracy of self-collected snorted and spit saliva in detecting COVID-19 using RT-PCR (ssRT-PCR) and lateral flow antigen test (ssLFA) versus nasopharyngeal swab RT-PCR (npRT-PCR).
Methods. One hundred ninety-seven symptomatic subjects for COVID-19 testing in a tertiary hospital underwent snort-spit saliva self-collection for RT-PCR and antigen testing and nasopharyngeal swab for RT-PCR as reference. Positivity rates, agreement, sensitivity, specificity, and likelihood ratios were estimated.
Results. Estimated prevalence of COVID-19 using npRT-PCR was 9% (exact 95% CI of 5.5% - 14.1%). A higher positivity rate of 13% in the ssRT-PCR assay suggested possible higher viral RNA in the snort-spit samples. There was 92.9% agreement between ssRT-PCR and npRT-PCR (exact 95% CI of 88.4% to 96.1%; Cohen’s Kappa of 0.6435). If npRT-PCR will be assumed as reference standard, the estimated Sensitivity was 83.3% (exact 95% CI of 60.8% to 94.2%), Specificity 93.9% (exact 95% CI of 89.3% to 96.5%), Positive predictive value of 57.7% (exact 95% CI of 38.9% to 74.5%), Negative predictive value of 98.2% (exact 95% CI of 95% to 99.4%), positive likelihood ratio of 3.65 (95% CI of 7.37 to 24.9), negative likelihood ratio of 0.178 (95% CI of 0.063 to 0.499). There was 84.84% agreement (95% exact CI of 79.1% to 89.5%; Cohen’s Kappa of 0.2356) between ssLFAvs npRT-PCR, sensitivity of 38.9% (exact 95% CI of 20.3% to 61.4%), specificity of 89.4% (exact 95% CI of 84.1% to 93.1%), PPV of 26.9% (95% CI of 13.7% to 46.1%), NPV of 93.6% (exact 95% CI of 88.8% to 96.4%), LR+ of 3.67 (95% CI of 1.79 - 7.51), LR – of 0.68 (95% CI of 0.47 - 0.99).
Conclusion. Our data showed that snort-spit saliva RT-PCR testing had acceptable diagnostic performance characteristics and can potentially be used as an alternative to the standard nasopharyngeal/oropharyngeal swab RT-PCR test for COVID-19 in certain situations. However, our data also showed that snort-spit saliva antigen testing using lateral flow assay did not offer acceptable performance.
		                        		
		                        		
		                        		
		                        			Saliva
		                        			;
		                        		
		                        			  SARS-CoV-2
		                        			;
		                        		
		                        			  Reverse Transcription
		                        			;
		                        		
		                        			  Reverse Transcriptase Polymerase Chain Reaction
		                        			
		                        		
		                        	
7.Community-oriented health care during a COVID-19 epidemic: A consensus statement by the PAFP task force on COVID-19
Maria Victoria Concepcion P. Cruz ; Karin Estepa-Garcia ; Lynne Marcia H. Bautista ; Jane Eflyn Lardizabal-Bunyi ; Policarpio B. Joves, Jr. ; Limuel Anthony B. Abrogena ; Ferdinand S. De Guzman ; Noel L. Espallardo ; Aileen T. Riel-Espina ; Anna Guia O. Limpoco ; Leilanie Apostol-Nicodemus ; Ma. Rosario Bernardo-Lazaro ; Ma. Louricha Opina-Tan
The Filipino Family Physician 2020;58(1):15-21
		                        		
		                        			Initial Planning:
		                        			Statement 1: A Community-oriented Health Care Plan that contains tasks and activities related to the community organization, environment, health care and social processes in order to mitigate the effect of the COVID-19 epidemic on the community should be developed. 
Statement 2: The plan should also include adjustments needed to continue the delivery of other health services i.e. maternal and child health, immunization, treatment of other communicable and non-communicable disease but with strict COVID-19 transmission precautions.
		                        		
		                        			Adjustment in the Community Organization and Environment:
		                        			Statement 3: A local task force should be organized to develop and implement the community health plan. The task force should be recognized and supported by the whole community.  
Statement 4: A facility in the barangay that can be used for isolation in case that a member will be diagnosed to have mild COVID-19. A hospital facility for referral of high-risk cases should also be identified and an emergency referral and transport plan should be established. 
Statement 5: All community health workers should wear appropriate personal protective equipment in the process of performing their community health work. 
Statement 6: Households in the community who have members at high-risk i.e. more than 60 years old, with existing chronic illness or other life-threatening condition should be identified and advised to take extra precautions i.e. personal hygiene, wearing mask and physical distancing. 
Statement 7: During the declared community quarantine period by the community or higher-level authority, all community members and household should be advised to stay at home, limit celebrations and community gatherings
		                        		
		                        			Performance of Routine Tasks and Activities:
		                        			Statement 8: A community-directed information, education and communication (IEC) plan should be developed and implemented for the following: a) Informing every household in the community on the basic and accurate information about COVID-19 and the community plan. b) Encouraging everyone to practice personal hygiene that includes regular and appropriate hand washing, daily bath, coughing and sneezing etiquette, wearing of mask, minimizing hand contact with eyes, nose and mouth and strict personal use of eating utensils, bath towels, etc. c) Encouraging everyone to clean everyday frequently touched surface like doorknobs, light and appliance control switch, gadgets, armchairs and tabletops. Cleaning agents can be ordinary detergents and water or 70% alcohol. d) Encouraging everyone to report and seek help to the community health worker if a household member is exposed and developed mild symptoms of COVID-19
		                        		
		                        			What to Do When a Member or Household is Exposed or Diagnosed COVID-19:
		                        			Statement 9: If there is a household whose member is exposed to a COVID-19, the person should be encouraged to stay home preferably in a room or area adequate for isolation, wear mask and maintain at least 2 meters physical distance from other family members. Statement 10: Other household members should be advised to watch out and monitor for the appearance of symptoms like fever, colds and cough. If the person is low risk but there is difficulty of breathing or worsening of symptoms or if the person is high risk i.e. elderly or with existing chronic disease and symptoms appear, they encouraged to inform the community health worker and facilitate the necessary referral and transport arrangement to the hospital. Call first before going. Statement 11: If the symptoms are mild, continue home isolation or in the isolation facility identified by the community, take over-thecounter medications like paracetamol for fever, increase water intake and ensure adequate nutrition, sleep and rest. Family members and community health workers are encouraged to provide psychological and social support to isolated patients. Discontinuation of isolation can be done if symptoms resolve within 14-21 days
		                        		
		                        			Epidemiology and Surveillance
		                        			Statement 12: The municipal or city health office should be provided daily with a situation report of the implementation of communityoriented health care for COVID-19. Situation report should include: a) The number of exposed, number of diagnosed cases, number of mild cases, number of cases referred to the hospital and number of cases recovered or died in the community. b) Brief description of best practices
		                        		
		                        		
		                        		
		                        			COVID-19
		                        			;
		                        		
		                        			 Noncommunicable Diseases
		                        			;
		                        		
		                        			 Quarantine
		                        			
		                        		
		                        	
8.Management of urethral atrophy after implantation of artificial urinary sphincter: what are the weaknesses?
Nathaniel H HEAH ; Ronny B W TAN
Asian Journal of Andrology 2020;22(1):60-63
		                        		
		                        			
		                        			The use of artificial urinary sphincter (AUS) for the treatment of stress urinary incontinence has become more prevalent, especially in the "prostate-specific antigen (PSA)-era", when more patients are treated for localized prostate cancer. The first widely accepted device was the AMS 800, but since then, other devices have also entered the market. While efficacy has increased with improvements in technology and technique, and patient satisfaction is high, AUS implantation still has inherent risks and complications of any implant surgery, in addition to the unique challenges of urethral complications that may be associated with the cuff. Furthermore, the unique nature of the AUS, with a control pump, reservoir, balloon cuff, and connecting tubing, means that mechanical complications can also arise from these individual parts. This article aims to present and summarize the current literature on the management of complications of AUS, especially urethral atrophy. We conducted a literature search on PubMed from January 1990 to December 2018 on AUS complications and their management. We review the various potential complications and their management. AUS complications are either mechanical or nonmechanical complications. Mechanical complications usually involve malfunction of the AUS. Nonmechanical complications include infection, urethral atrophy, cuff erosion, and stricture. Challenges exist especially in the management of urethral atrophy, with both tandem implants, transcorporal cuffs, and cuff downsizing all postulated as potential remedies. Although complications from AUS implants are not common, knowledge of the management of these issues are crucial to ensure care for patients with these implants. Further studies are needed to further evaluate these techniques.
		                        		
		                        		
		                        		
		                        			Atrophy
		                        			;
		                        		
		                        			Humans
		                        			;
		                        		
		                        			Postoperative Complications/therapy*
		                        			;
		                        		
		                        			Prosthesis Failure
		                        			;
		                        		
		                        			Prosthesis Implantation
		                        			;
		                        		
		                        			Prosthesis-Related Infections/therapy*
		                        			;
		                        		
		                        			Urethra/pathology*
		                        			;
		                        		
		                        			Urethral Diseases/therapy*
		                        			;
		                        		
		                        			Urethral Stricture/surgery*
		                        			;
		                        		
		                        			Urinary Incontinence, Stress/surgery*
		                        			;
		                        		
		                        			Urinary Sphincter, Artificial
		                        			
		                        		
		                        	
9.Ministry of Health Clinical Practice Guidelines: Chronic Obstructive Pulmonary Disease.
Tow Keang LIM ; Cynthia B CHEE ; Patsy CHOW ; Gerald Sw CHUA ; Soo Kiang ENG ; Soon Keng GOH ; Kwee Keng KNG ; Wai Hing LIM ; Tze Pin NG ; Thun How ONG ; S T Angeline SEAH ; Hsien Yung TAN ; K H TEE ; Vimal PALANICHAMY ; Meredith T YEUNG
Singapore medical journal 2018;59(2):76-86
		                        		
		                        			
		                        			The Ministry of Health (MOH) has updated the Clinical Practice Guidelines on Chronic Obstructive Pulmonary Disease (COPD) to provide doctors and patients in Singapore with evidence-based treatment for COPD. This article reproduces the introduction and executive summary (with recommendations from the guidelines) from the MOH Clinical Practice Guidelines on COPD, for the information of SMJ readers. Chapters and page numbers mentioned in the reproduced extract refer to the full text of the guidelines, which are available from the Ministry of Health website: https://www.moh.gov.sg/content/moh_web/healthprofessionalsportal/doctors/guidelines/cpg_medical.html. The recommendations should be used with reference to the full text of the guidelines. Following this article are multiple choice questions based on the full text of the guidelines.
		                        		
		                        		
		                        		
		                        			Adult
		                        			;
		                        		
		                        			Aged
		                        			;
		                        		
		                        			Evidence-Based Medicine
		                        			;
		                        		
		                        			Humans
		                        			;
		                        		
		                        			Middle Aged
		                        			;
		                        		
		                        			Palliative Care
		                        			;
		                        		
		                        			Prevalence
		                        			;
		                        		
		                        			Pulmonary Disease, Chronic Obstructive
		                        			;
		                        		
		                        			diagnosis
		                        			;
		                        		
		                        			therapy
		                        			;
		                        		
		                        			Pulmonary Medicine
		                        			;
		                        		
		                        			standards
		                        			;
		                        		
		                        			Quality Improvement
		                        			;
		                        		
		                        			Radiography, Thoracic
		                        			;
		                        		
		                        			Risk Factors
		                        			;
		                        		
		                        			Singapore
		                        			;
		                        		
		                        			Steroids
		                        			;
		                        		
		                        			therapeutic use
		                        			
		                        		
		                        	
10.Circulation and etiological characterization of Salmonella enterica serotype in human in Guangdong province, 2007-2016.
B X KE ; H H ZENG ; D M HE ; H L TAN ; B S LI ; Y H LIANG ; C W KE
Chinese Journal of Epidemiology 2018;39(1):63-66
		                        		
		                        			
		                        			<b>Objective:b> To understand the circulation, drug resistance and molecular characteristics of Salmonella1, 4, [5], 12: i:- in human in Guangdong province. <b>Methods:b>Salmonella1, 4, [5], 12: i:- isolated from diarrhea patients in Guangdong during 2007-2016 were detected for drug resistance, genes and PFGE characteristics. <b>Results:b> A total of 2 960 strains Salmonella1, 4, [5], 12: i: - were isolated from human diarrhea cases during this period. The positive rates of the isolation increased year by year. The male to female ratio of the infection cases was 1.58∶1, and the infection mainly occurred in infants and young children. Except imipenem, Salmonella1, 4, [5], 12: i: - was resistant to other 17 antibiotics to some extent. The drug resistant rates to ceftazidime, cefotaxime and ciprofloxacin increased from 2011 to 2016. Multi-drug resistance was serious, for example, the multi-drug resistant strains with ASSuT accounted for 70.62% (435/616) and the multi-drug resistant strains with ACSuGSTTm accounted for 27.11% (167/616). The lack of fljA, fljB and hin genes, as well as the retaining of iroB, STM2740, STM2757 genes, resulted in the unable expression of FljBenx gene with 8 different defection profiles. There were 934 different PFGE patterns observed in 2 347 strains, which displayed a relatively large fingerprint polymorphism. The major PFGE pattern was JPXX01. GD0226, which was found in 97 strains, accounting for 4.13% (97/2 347). The PFGE patterns in 168 Salmonella1, 4, [5], 12: i: - strains were consistent with that of Salmonella typhimurium. <b>Conclusions:b>Salmonella1,4,[5], 12: i: - strains has become the major serotype of Salmonella that cause diarrhea in human in Guangdong. The multi-drug resistance of Salmonella1,4, [5], 12: i: - was serious, and since the defection of fljA, fljB and hin genes, the expression of FljBenx protein failed. The PFGE results were diverse, which displayed polymorphism in inheritance.
		                        		
		                        		
		                        		
		                        			Adolescent
		                        			;
		                        		
		                        			Adult
		                        			;
		                        		
		                        			Aged
		                        			;
		                        		
		                        			Aged, 80 and over
		                        			;
		                        		
		                        			Anti-Bacterial Agents/pharmacology*
		                        			;
		                        		
		                        			Child
		                        			;
		                        		
		                        			Child, Preschool
		                        			;
		                        		
		                        			China/epidemiology*
		                        			;
		                        		
		                        			Diarrhea/microbiology*
		                        			;
		                        		
		                        			Drug Resistance, Bacterial
		                        			;
		                        		
		                        			Electrophoresis, Gel, Pulsed-Field
		                        			;
		                        		
		                        			Female
		                        			;
		                        		
		                        			Humans
		                        			;
		                        		
		                        			Infant
		                        			;
		                        		
		                        			Male
		                        			;
		                        		
		                        			Microbial Sensitivity Tests
		                        			;
		                        		
		                        			Middle Aged
		                        			;
		                        		
		                        			Salmonella Infections/prevention & control*
		                        			;
		                        		
		                        			Salmonella enterica/isolation & purification*
		                        			;
		                        		
		                        			Salmonella typhimurium
		                        			;
		                        		
		                        			Serogroup
		                        			;
		                        		
		                        			Serotyping
		                        			;
		                        		
		                        			Young Adult
		                        			
		                        		
		                        	
            

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