Objective: To screen out the long non-coding RNAs (LncRNAs) related to bone morphogenetic protein-9 (BMP-9) expression, and to investigate the role of BMP-9 in the growth, differentiation, migration and apoptosis of breast cancer cells by regulating the expression of LncRNA. Methods: MDA-MB-231 cells were infected with the recombinant adenovirus Ad-BMP-9 carrying whole BMP -9 gene (named as MDA-MB-231/BMP-9 cells) or the empty vehicle adenovirus Ad-GFP carrying green fluorescent protein (GFP) gene (named as MDA-MB-231/GFP cells), respectively. Microarray technology was used to detect the difference in LncRNAs expression between MDAMB- 231/BMP-9 and MDA-MB-231/GFP cells. The changes of LncRNA LINC00443, LINC00638, LINC00486, RHNO1, SERHL, HOXA11-AS, IQCA1, LINC00461, LOC440173, LHFPL, ANKRD36BP2, BVES-AS1, LINC00937 and LINC00608 were validated by real-time fluorescent quantitative PCR. The biological funcation and related pathways of the above LncRNAs were analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Results: The expression level of BMP-9 mRNA in MDA-MB-231/BMP-9 cells was significantly higher than that in MDA-MB-231/GFP cells (as the control). Compared with the control group, the expression levels of LncRNAs in MDA-MB-231/BMP-9 cells changed significantly. The expression levels of LINC00443, LINC00638 and LINC00486 were up-regulated, while the expression levels of IQCA1, LINC00461, LOC440173, LHFPL and ANKRD36BP2 were down-regulated. The altered LncRNAs participated in the formation of cytoskeleton, cell membrane and so on, or played roles as signal molecules in intercellular signal transduction. Conclusion: The expression profile of LncRNAs in MDA-MB-231 cells with BMP-9 overexpression is significantly changed, indicating that LncRNAs may play key roles in the proliferation and invasion of breast cancer MDA-MB-231 cells regulated by BMP-9.

Objective: To summarize the clinical characteristics and therapeutic experiences of the middle ear teratoma in infants. Methods: Three cases of middle ear teratoma, from 2012-2015 in Beijing Friendship Hospital were analyzed. Results: The three cases all developed slowly and presented unilateral otorrhea and hearing loss. Otoscopy showed the granulation tissue in the external ear canal. Audiological changes varied according to the degree of severity. Imaging features showed the pocket-like occupancy lesions in the Eustachian tube area. The temporal bone CT showed mass with soft tissue density usually involved in the mastoid and tympanic cavity. MRI showed mixed signal intense on both T1 and T2 weighted imaging. All the three cases received neoplasm resection of the middle ear. Only one case received tympanoplasty surgery at the same time. And all the pathology results displayed mature teratoma. The follow-up time was 17 to 54 months. MRI showed complete removal of the tumor. Conclusions Teratoma are rare in the head and neck neoplasm. When the infants suffer from the unilateral otorrhea, hearing loss, and granulation tissue formed in the external ear canal, it should be vigilant for teratoma. The differential diagnosis is middle ear cholesteatoma, congenital first branchial cyst or fistula, and middle ear carcinoma. Temporal bone CT combined with MRI could improve the accuracy of diagnosis. It should be totally resection as soon as possible if there is no contraindication. Postoperative follow-up and imaging examination are necessary to eliminate tumor recurrence.

Objective To investigate the value of high resolution CT (HRCT) in displaying the anatomic relationship between labyrinth segment of facial canal and cochlea.Methods Totally 110 patients (220 ears) who underwent HRCT were collected.The original images were transferred to workstation for image processing.MPR images were acquired.The anatomic relationship between labyrinth segment of facial canal and cochlea was observed in oblique coronal MPR images.The bony septum between labyrinth segment of facial canal and cochlea was assessed as definite defect (Type Ⅰ),doubtful defect (Type Ⅱ) or complete (Type Ⅲ),respectively.Results There were 71 ears (71/220,32.27％) of Type Ⅰ,diameters of bone fissure ranged from 0.3-1.3 mm (average diameters [0.64±0.26]mm),86 ears (86/220,39.09％) of Type Ⅱ and 63 ears (63/220,28.64％) of Type Ⅲ,with bony septum thickness ranged from 0.3-1.0 mm (average thickness [0.68±0.15]mm).No statistical difference of rates of the above three types was found between different genders,among age groups and between both side of ears (all P＞0.05).Conclusion HRCT is a reliable method to show the anatomic relationship between labyrinth segment of facial canal and cochlea.

60 curved root canals of permanent teeth with angles of curvature ranging from 15°to 40°(Schneider's methodology)were prepared using the instruments of Hyflex CM(HC) and ProTaper(PT) Universal respectively(n=30).Using standardized pre-and post-instrumentation paralleling periapical radiographs,canal curvature was determined by image analysis software and the clinical shaping effect of Hyflex CM and ProTaper rotary NiTi files were compared.The canal curvature in group HC and PT decreased by 4.54°±3.25° and 5.63°±3.84° respectively(between pre-and post-treatment in both groups,P<0.05;between groups,P>0.05).Hyflex CM can meet the clinical necessity for the instrumentation of curved root canals.

Hematogenous metastasis is common in lung cancer, and except for small cell lung cancer and melanoma, tumors with extensive metastasis seldom involve the prostate. The treatment of lung adenocarcinoma with prostate metastasis relies mainly on systemic chemotherapy with rigorous treatment of the primary lesions and metastatic palliative radiotherapy. Comprehensive treatment integrating traditional Chinese medicine may improve the quality of life of the patients.
Adenocarcinoma ; pathology ; Aged ; Humans ; Lung Neoplasms ; pathology ; Male ; Neoplasm Metastasis ; Prostatic Neoplasms ; secondary

Hematogenous metastasis is common in lung cancer, and except for small cell lung cancer and melanoma, tumors with extensive metastasis seldom involve the prostate. The treatment of lung adenocarcinoma with prostate metastasis relies mainly on systemic chemotherapy with rigorous treatment of the primary lesions and metastatic palliative radiotherapy. Comprehensive treatment integrating traditional Chinese medicine may improve the quality of life of the patients.

Hematogenous metastasis is common in lung cancer, and except for small cell lung cancer and melanoma, tumors with extensive metastasis seldom involve the prostate. The treatment of lung adenocarcinoma with prostate metastasis relies mainly on systemic chemotherapy with rigorous treatment of the primary lesions and metastatic palliative radiotherapy. Comprehensive treatment integrating traditional Chinese medicine may improve the quality of life of the patients.

OBJECTIVE: To improve the possibility of diagnosis of the Mondini dysplasia. METHOD: The clinical manifestation and the examination of CT and MRI and surgical treatment of the Mondini dysplasia were discussed. RESULT: Because Mondini dysplasia with cerebrospinal fluid leak would occur recurrent meningitis, the patients were often difficult to be diagnosed. Especially. if the defect was unilateral, it was frequently unrecognized. CONCLUSION The patients with recurrently unclear cerebrospinal fluid leak and meningitis would be suspected. The diagnosis of the disease is based on the examination of the temporal bone CT and MRI. To the patients with cerebrospinal fluid leak, a transtympanic closure is one of very effective management.
Adolescent ; Cerebrospinal Fluid Otorrhea ; diagnosis ; etiology ; surgery ; Ear, Inner ; abnormalities ; Humans ; Male ; Tympanoplasty ; methods

OBJECTIVE To investigate the distribution and drug resistance status to pathogens from urinary tract and offer scientific evidence for reasonable usage of antibiotics.METHODS Totally 389 isolates derived from infected urinary tract in mountain area of western Hubei Province from 2002 to 2005 were identified and antibiotic susceptibility test was performed.RESULTS Escherichia coli accounted for 66.1% ranking the first.The isolates were all susceptible to imipenem and vancomycin.The ratio of ESBLs in E.coli and Klebsiella pneumoniae was 23.7% and 25.9%,respectively.CONCLUSIONS The drug resistance rate in isolates derived from infected urinary tract of patients in mountain area was lower than in plain region of Hubei Province.

To observe the glial reactions surrounding facial motor neurons following facial nerve anastomosis. At 1,7,21 and 60 d following facial nerve anastomosis, the recovery process of facial movement was observed, the glial fibrillary acidic protein (GFAP) immunoreactivitywas analyzed by a combined method of fluorescent retrograde tracing and immunofluorescent histochemical stai ning, and the ultrastructure of astrocytes were observed under a transmission electron microscope (TEM), respectively. Postoperatively the function of facial muscles could not return to normal, often accompanied with hyperkinetic syndromes such as synkinesis at the late stage. Motor neurons in every facial subnucleus could be retrogradely labeled by fluoro gold (FG), and displayed an evident somatotopic organization. Normally there was a considerable number of GFAP-positive cells in nonnucleus regions but few inside the facial nucleus region. Postoperatively the GFAP immunoreactivity in the anastomotic side increased significantly, but gradually decreased at the late stage. The ultrastructure of astrocytes in our experiment showed that the sheet-like process of astrocytes invested and protected the injured facial motor neurons. The present study shows that reactive astrocytes undergo some characteristic changes during the process of facial nerve injury and regeneration. The plastic change at the late stage may be involved in the mechanism of synkinesis.