Objective To investigate the current situation of fertility intention of nurses of childbearing age in Chongqing,and to explore the related factors affecting their fertility intention.Methods A total of 509 in-service nurses(39 males and 470 females)from 12 hospitals in Chongqing were selected as the research ob-jects,and a questionnaire survey was conducted on their fertility intention.Univariate analysis and logistic re-gression analysis were performed on the relevant variables.Results Only nine people(1.77％)had the will-ingness to have a third child.Among them,the single influencing factors of the willingness to have a third child were parents living together(P=0.043),hospital category(P=0.013),and gender(P=0.025).The respondents who clearly stated that they did not want to have three children believed that fertility pressure came from children's education investment,children's medical expenses,elderly medical expenses and insur-ance investment,in 65.80％of the families,the investment in children's education accounts for ≥10％of the annual family income.The log-binomial regression analysis of the fertility behavior of the second child showed that the fertility behavior of the second child of the intermediate title was 2.41 times that of the primary title(P＜0.01),the non-parental living was 0.41 times that of the parents living together(P＜0.01),the contract system was 0.40 times that of the formal establishment(P＜0.01),and the annual household income of＞500 000 yuan was 6.09 times that of 0 to 100 000 yuan(P＜0.05).Conclusion The fertility intention of the three-child childbearing age group of licensed nurses in Chongqing is weak,and economic factors and educa-tional pressure are the main factors affecting the fertility intention.

Objective To explore and construct an indicator system for performance appraisal in operating room nurses of cancer hospitals,in order to provide references for performance management.Methods From May to December 2022,the four-dimensional model of individual performance was used as the theoretical framework,and the research was carried out by literature research method,focus group interview method,Delphi expert consultation method and analytic hierarchy process,to establish the content of the performance appraisal index system of operating room nurses in cancer hospitals and the weight of each index.Results After 2 rounds of expert correspondence,29 questionnaires were sent out and 27 returned in the first round,with an expert positive coefficient of 93.10％;27 questionnaires were sent out and 27 returned in the second round,with an expert positive coefficient of 100％;expert authority coefficient of the first round was 0.941 and the second round was 0.937.In the first round,the variation coefficient of each index was 0-0.249,and the coordination coefficient was 0.201-0.352;in the second round,the variation coefficient of each index was 0-0.204,and the coordination coefficient was 0.275-0.407.Finally,a performance appraisal index system of operating room nurses in cancer hospitals was formed,which consisted of 4 first-level indicators,including task performance,interpersonal performance,adaptive performance and effort performance,11 second-level indicators and 54 third-level indicators.Conclusion The indicator system for performance appraisal in operating room nurses of cancer hospitals constructed in this study is reliable and scientific,specialized and applicable,and it can provide references for performance management.

Objective:To evaluate radiofrequency ablation-assisted liver resection on early recurrence of hepatocellular carcinoma(HCC) with microvascular invasion (MVI).Methods:A total of 82 HCC patients from Jun 2015 to Jun 2020 were divided into assisted group ( n=41) and control group ( n=41) after local hepatectomy.And by pathology,both groups were further substratified into with or without MVI subgroups. Results:There was no statistically significant difference in the baseline data between two groups,nor there was difference in recurrence-free survival rate between the two groups ( χ 2=0.177, P=0.674). However, by subgroup analysis, the recurrence-free survival rate of ablation assisted group was higher than that of the simple local hepatectomy group among MVI positive patients ( χ 2=5.096, P = 0.024).Multivariate analysis showed that only tumor diameter ( HR=1.32, 95% CI: 1.02-1.72, P=0.036) was an independent risk factor for local recurrence at the incisal margin, while mode of operation ( HR=0.15 ,95% CI: 0.04-0.52 ,P=0.003) and MVI ( HR=8.65 ,95% CI: 2.19-34.19 ,P=0.002) were independent risk factors for intrahepatic distant metastasis. Conclusion:Local hepatectomy assisted by intraoperative radiofrequency ablation on hepatic cross section could effectively reduce the postoperative early recurrence rate for hepatocellular carcinoma patients with MVI.

Objective:To analyze the clinical and gene mutation characteristics of congenital disorder of glycosylation (CDG) caused by compound heterozygous mutation of the COG6 gene ( COG6-CDG). Methods:This study retrospectively analyzed the clinical data and genetic test results of a patient with COG6-CDG in Bayi Children's Hospital, the Seventh Affiliated Medical Center of Chinese PLA General Hospital, in August 2019. Literature was retrieved with keywords including COG6, COG6-CDG, congenital disorders of glycosylation typeⅡL and congenital disorders of glycosylationⅡL in China National Knowledge Infrastructure, Wanfang Database, VIP Database, PubMed, and Web of Science Database from the establishment to July 2020, to summarize the clinical and genetic characteristics of COG6-CDG. Results:(1) Case report: The 59-day-old baby boy, with a gestational age of 27 +5 weeks and birth weight of 1 180 g, presented with multi-system involvement on admission, including unidentified progressive hepatosplenomegaly with jaundice and ascites, persistent thrombocytopenia, microcephaly, hypotonia, hypohidrosis, hyperkeratosis, and recurrent hyperthermia, infection, and hypoglycemia, as well as dysfunctions of the heart, gastrointestinal tract, lungs, kidneys, ocular fundus, and the coagulation system. Despite given ventilator-assisted ventilation, anti-infection therapy, abdominal puncture and drainage, and blood transfusion, the patient still had an aggravated condition and eventually died of multiple organ failures 192 d after birth. Genetic analysis showed that the nuclear family carried compound heterozygous mutations in the COG6 gene (NM_020751.2), including missense mutations of c.662C>T(p.T221M) in exon 7 and c.443T>C(p.I148T) in exon 5, which were both novel mutations and originated from the mother and father, respectively. (2) Literature review: Eight related papers were retrieved, including 20 cases. The main manifestations were various degrees of nervous system abnormalities and growth retardation, complicated by abnormalities of the liver, heart, gastrointestinal tract, blood, immunity, teeth, and bones. All the reported cases suffered from mental and growth retardation, and nine deaths were reported. A total of 11 COG6 gene mutations were identified, and most of them were c.1167-24A>G splicing mutations in a deep intron (seven cases), followed by c.1646G>T (four cases) and c.511C>T (three cases). Conclusions:COG6-CDG commonly manifests as multi-system and multi-organ dysfunctions with poor prognosis. Gene detection is conducive to the accurate diagnosis of COG6-CDG. Our case carries compound heterozygous mutations of c.662C>T(p.T221M) and c.443T>C(p.I148T), which are unreported novel mutations.

Purpose: Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. Materials and Methods: In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. Results: The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. Conclusion Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.

Objective:To explore the influencing factors of satisfaction with thyroid nodule fine needle biopsy (FNA).Methods:The ultrasonic images of 88 patients with thyroid nodules (95 nodules) in Dongguan Binhai Bay Central Hospital from September 2016 to January 2019 were analyzed retrospectively. According to Bethesda cytological classification standard, Bethesda class Ⅰ cytological specimens were defined as unsatisfactory, and Bethesda class Ⅱ-Ⅵ cytological specimens were defined as satisfactory. The thyroid nodule location, composition, internal echo, morphology, aspect ratio, calcification, blood supply mode and American College of Radiology Thyroid Imaging Reporting and Data System(ACR TI-RADS) classification on the satisfaction rate of thyroid nodule FNA samples were evaluated, and then the meaningful influencing factors were analyzed by logistic regression.Results:The overall satisfaction of the sample in this study was 84.2% (80/95). The results of univariate analysis showed that the location, aspect ratio and ACR TI-RADS classification of thyroid nodules were the related factors affecting the satisfaction of FNA specimens of thyroid nodules, with statistically significant difference ( P<0.05). Logistic regression analysis showed that the aspect ratio of thyroid nodules was an independent factor affecting the satisfaction of FNA specimens of thyroid nodules ( OR=13.5, P=0.02). Conclusions:The main factor affecting the satisfaction rate of FNA specimens of thyroid nodules may lie in the characteristics of the aspect ratio of the nodules themselves. Optimizing the puncture route may help to reduce the dissatisfaction rate of FNA specimens of thyroid nodules.

Objective:To explore the application effect of online teaching supervision in nursing teaching quality of undergraduate nursing students.Methods:Using the convenient sampling method, the 2017 (September 2019 to January 2020) and 2018 (March to July 2020) undergraduate nursing students from the School of Nursing of Harbin Medical University were selected as the research objects. The 2018 undergraduate nursing students were included in the online live teaching group ( n=80) , and the 2017 undergraduate nursing students were included in the offline teaching group ( n=76) . A total of 10 supervisors were selected to evaluate the teaching quality of teachers' online and offline classes through the Multiple Comprehensive Evaluation Tables. The results of the final examination of nursing students in the two groups were used to evaluate the effect of the online live teaching form. The teaching satisfaction with the online live teaching method of nursing students in the two groups was compared. Results:The online teaching scored (95.05±2.14) on the Multiple Comprehensive Evaluation Tables, which was higher than that of the offline teaching (90.05±0.96) , and the difference was statistically significant ( t=6.745, P<0.01) . There were no statistically significant differences in the final exam scores of nursing students in the two groups and their satisfaction with online live teaching methods ( P>0.05) . Conclusions:The practice form of online classroom teaching supervision can effectively ensure the teaching quality and teaching effect of teachers and ensure the teaching satisfaction of nursing students with online live teaching.

Objective: To analyze the epidemiological characteristics and associated factors of delayed voiding among middle school students, and to provide the basis for health education. Methods: A total of 1 260 middle school students were selected from Sanmen country in Taizhou by using multi-stage stratified sampling method. A cross-sectional survey was conducted to investigate the demographic characteristics, epidemiological characteristics and associated factors of delayed voiding by questionnaire. Results: The proportion of frequent delayed voiding among middle school students was 14.37%(181). The proportion among senior high school students was 15.51%(90), and among junior high school students was 13.67%(91)，no significant differences were found by grade(χ2=0.57, P=0.45). The multivariate Logistic regression analysis showed that residential school, homework per day >2 hours, games and reduce the frequency of drinking ≥7 times/week were risk factors for delayed voiding frequently in junior high school students(OR=3.71, 1.70, 3.02,6.52，P<0.05). Higher than 2 hours of daily homework, a supportive or indifferent attitude to delayed voiding, staying in bed after waking up, drinking <0.5 L/d or ≥2.0 L/d, and reduce the frequency of drinking ≥7 times/week were risk factors for delayed voiding behavior in high school students(OR=2.23, 1.81, 2.22, 3.48, 2.69, 3.00, P<0.05). Conclusion The prevalence of frequent delayed voiding was high among middle school students. Attitude, environment and the quality of drinking water were the primary factors of frequent delayed voiding.

Objective:To analyze the literature on stigma of foreign patients with epilepsy, to understand the research hotspots and research frontiers in this field, and to provide reference for the research in this field in China.Methods:The data was analyzed by HistCite software by searching the "Web of Science TM Core Collection" database. Results:A total of 740 related articles were retrieved, and the number of publications was on the rise. Jacoby A was a high-yielding author. The EPILEPSY & BEHAVIOR journals had the largest number of publications. The United States and the United Kingdom were leading in this field. The research institutes were mainly universities. The research hotspots focus on four aspects: research population, quality of life, knowledge attitude, and influencing factors.Conclusion:The study of stigma in patients with epilepsy is receiving increasing attention from the international community, but there is still a large gap between China and the leading countries in this field. Domestic scholars can learn from foreign research directions and methods to explore the level of stigma, influencing factors and effective intervention programs to provide patients with better treatment results and improve patient quality of life.

Objective: To explore the degree of social support in patients after Coronary Artery Bypass Grafting(CABG) and its influence on Acute Stress Disorder (ASD), in order to provide theory evidence for nursing intervention. Methods: From June 2017 to Mar 2018, 359 patients after CABG in Tangshan Workers Hospital were recruited for the study. Their general information were collected, SASRQ, SSRS and PSS-Fa were used for professional evaluation. Results: The degree of social support in patients after CABG was at medium level, the total score of social support was (41.69±0.28), higher than the normal domestic value[(34.56±3.73)score, n=128]; character (F=29.652), employment (t=6.526)and the type of health insurance (F=20.547)impacted on the degree of social support (P<0.05). Total score of social support had negative correlation with SASRQ total score (r=-0.528, P<0.05); character (β=0.262), family support (β=-0.281) and social support (β=-0.267) were picked in the regression equation (P<0.05), the results showed that introversion was a risk factor for ASD in patients after CABG, high degree of family support and social support were protective factors for ASD in patients after CABG. Conclusions Social support of patients after CABG needs to be improved, so as to reduce the incidence of acute stress disorder.