【Objective】 To compare the clinical value of magnetic-controlled capsule endoscopy （MCE） and traditional capsule endoscopy （CE） in the diagnosis of intestinal diseases in hospitalized patients. 【Methods】 A single-center retrospective study was conducted in 263 inpatients who underwent MCE and CE in The First Affiliated Hospital of Xi’an Jiaotong University from March 2016 to March 2020. The information included the patients’ general data， chief complaints， and results of capsule endoscopic examination. 【Results】 ① The overall detection rate in small intestinal diseases was 74.45% in MCE group and 73.81% in CE group， respectively （P=0.905）. The three most common diseases in the two groups were erosive/ulcerative lesions， vascular lesions， and lymphangiectasia. ② The endoscopic auxiliary rate was significantly lower in MCE group than in CE group （0% vs. 9.49%， P<0.001）. ③ There was no significant difference in the rate of intestinal incompletion between the two groups （7.94% vs. 13.87%， P=0.185）. 【Conclusion】 MCE is similar to CE in the diagnostic value for intestinal diseases. Currently， it can be used as one of the methods of small intestinal examination， but this needs to be supported by more multicenter and sizable simple studies.

【Objective】 To explore the application value of preoperative gastroscopic carbon nanoparticles labeling in patients undergoing laparoscopic radical gastric cancer surgery. 【Methods】 We included cases of laparoscopic radical gastric cancer surgery at The First Affiliated Hospital of Xi’an Jiaotong University from January 2017 to December 2019. Some cases received submucosal injection of carbon nanoparticles under the gastroscope before surgery. The effects of carbon nanoparticles labeling on the number of lymph nodes detected， operation duration and surgical complications were compared and analyzed. 【Results】 A total of 397 patients undergoing laparoscopic radical gastric cancer surgery were enrolled. Among them， 78 cases underwent gastroscopic carbon nanoparticles tracer labeling before operation. No complications were observed. The total number of lymph nodes detected by pathology after surgery in the carbon nanoparticles group significantly increased ［（22.0（4.0） vs. 22.0（3.0）， P=0.033）］ while the operation time significantly reduced ［（185.0±37.48）min vs. （213.4±23.66）min， P<0.001］ compared with those in the control group. New gastric cancer lesions were revealed by gastroscopy in three cases （3.8%） of carbon nanoparticles labeling， and the original planned operation method was changed in two cases （2.6%）. 【Conclusion】 Preoperative endoscopic carbon nanoparticles tracer labeling can not only help shorten the time of laparoscopic radical surgery for gastric cancer and increase the number of total lymph nodes detected for more accurate TNM staging， but also provide an opportunity for the discovery of synchronous multiple gastric cancer.

According to the training requirement of the biological-psycho-social medical model to the clinical medical students,in order to promote the comprehensive improvement of medical students' professional knowledge,hands-on ability and human qualities,we design the course of introduction to clinical medicine.Through six major functional modules such as basic professional quality,clinical diagnosis basic technology,basic skills related to the operation,the new progress in clinical medicine and technology,clinical nursing and medical relationship,and medical information management,we build up the core content of integrated course of introduction to clinical medical.In this way,the clinical and related basic knowledge and skills are integrated,the clinical course is closely connected with the basic curriculum,the medical and the humanities exchanges.Through this design,the foundation is laid out for the collaborative efforts of the organ-system of integrated curriculum reform.

Objective To observe the change of circulating microRNAs (miRNAs),regulatory mechanism in patients with atrial fibrillation (AF) before and after radiofrequency ablation (RFA).Methods From Jauuary 2011 to December 2013,peripheral blood samples were taken from 30 AF patients (10 paroxysmal,10 persistent and 10 permanent AF) before and 3 months after RFA.The total RNA was extracted and hybridized with the miRNA chips,and the differential expression of miRNA and clustering analysis in whole genome were made with Volcano Plot and tMEV software respectively,and validated by real-time PCR.The target gene analysis of miRNAs was predicted through the Mirbase,Miranda and Targetscan databases.Results were compared with those from 10 heahhy subjeets (control group).Results Compared with control group,the expressions of 25 miRNAs were down-regulated before RFA and up-regulated after RFA in AF group,while other 40 miRNAs expression ehauged in the opposite way;among them,the expressions of 7 miRNAs including miR-199a-3p/miR-199b-3p were down-regulated ＞ 1.5-fold before RFA and up-regulated ＞ 100-fold after RFA;oppositely,6 miRNAs including miR-BART8-3p were up-regulated ＞ 1.5-fold before RFA and down-regulated ＞ 10-fold after RFA.Interestingly,6 miRNAs including miR-30b-5p,which were involved in AF-related electrical aud structural remodeling,were down-regulated ＞5-fold before RFA,but up-regulated ＞ 50-fold after RFA.Four miRNAs including miR-377-5p,which were involved in the regulation of CACNA1C ICa L channel protein,were different before and after RFA.Conclusion miRNAs regulate the occurrence and development of AF.RFA can change the expression of miRNAs in AF patients,which may be important for reversing the electrical and structural remodeling and maintaining sinus rhythm after RFA.miRNAs,such as miR-30b-5p,miR-377-5p and miR-199a-3p/miR-199b-3p etc.,might become the target markers for early diagnosis and intervention of AF in future.

BACKGROUND:How to reduce the incidence and mortality of cardiovascular diseases is an urgent concern in the field of public health.
OBJECTIVE:To explore the influence of adenovirus-mediated NSF-siRNA release from vesoactive substance on the cardiac function of a rat model of myocardial infarction.
METHODS:A total of 36 adult Sprague-Dawley rats were applied to establish acute myocardial infarction models by ligating the anterior descending branch of the left coronary artery. After the model was determined by electrocardiogram successful y, NSF-siRNA adenovirus (experimental group), negative adenovirus (control group) and normal saline (normal saline group) were injected near the infarct area of the left ventricle of rats respectively. After 2 weeks, the left ventricular ejection fraction (LVEF) was tested with noninvasive ultrasonic cardiogram. Meanwhile, the left ventricular end-diastolic pressure (LVEDP) and maximum pressure rising speed of left ventricular (dp/dt max) were detected by connecting the right external carotid artery place pipe to the BL-420 biological function experiment system, to evaluate the cardiac function. Subsequently, the rat heart was harvested for serial sections to observe the infarcts range.

Objective To investigate the correlation between plasma brain natriuretic peptide (BNP) ,central aortic systolic pres-sure with the degree of coronary artery lesion .Methods One hundred and fifty patients with coronary artery disease ,positive coro-nary angiographic results and without heart failure in the cardiological department of this hospital from March to June 2011 were selected and divided into the hypertension group (n=90) and the non-hypertension group(n=60) according to the blood pressure . The plasma BNP before angiography was detected by ELISA .The coronary lesion vessels and clinical scores were assessed after an-giography .The central aortic pressure before angiography was measured by the noninvasive measurement method and the diastolic blood pressure(DBP) ,systolic blood pressure(SBP) and pulse pressure(PP)were recorded .The correlation between PP and BNP was analyzed by Logistic regression .Results The plasma BNP concentration in the hypertension group was significantly higher than that in the non-hypertension group(P<0 .05) .The SBP level in 2 vessels ,3 vessels was significantly higher than that in the momal coronary group(P<0 .05) ,the PP in 3 vessels was significantly higher than that in the momal coronary group (P<0 .05) . The BNP level in 3 vessels ,2 vessels and single vessel of coronary artery lesion was significantly higher than that in the normal cor-onary artery group(P<0 .05) .The Logistic regression analysis on the PP influencing factors found that PP was closely related with the number of coronary artery lesion vessels ,lesion score ,LVEF and BNP ;the multiple correlation coefficient between PP with the number of coronary artery lesion vessels ,lesion score and BPN was 0 .91 ,its linear model was PP=0 .543 lesion vessels number +0 .656 lesion score + 0 .864 BNP .Conclusion PP of the central aortic pressure is a risk factor for the development and progress of coronary artery stenosis occurrence .BNP may be used as a plasma marker of the degree of coronary artery stenosis .

Objective To compare the predicting values for Prognosis among Global Risk Classification (GRS),Synergy Between Percutaneous Coronary Intervention With TAXUS and Cardiac Surgery (SYNTAX) score,the European System for Cardiac Operative Risk Evaluation (EuroSCORE) in patients who received stenting because of unprotected left main coronary artery (ULMCA) lesion.Methods Totally 105 successive elderly patients with ULMCA lesion who received stenting were divided into 2 groups:with and without main adverse cardiac events (MACE).The clinical and angiographic characteristics were analyzed and then compared among GRC,SYNTAX score and EuroSCORE.Results As compared with none MACE group,MACE group had higher EuroSCORE score (2.0±2.3 vs.6.5±2.9,t=8.18,P=0.002),and more trivessel disease and left main bifurcation lesion (x2 =8.96,6.96,P =0.011,P =0.008).High risk GRC showed more MACE than medium or low risk GRC [55.9％ (19/34) vs.20.5％(9/44),7.4％ (2/27),x2 =19.77,P=0.001].AUC(95％CI )of GRC,SYNTAX score and EuroSCORE were [0.821 (0.730-0.912),0.586(0.462-0.709) and 0.631 (0506-0.757)],respectively.Compared with SYNTAX score and EuroSCORE,GRC was superior in the MACE predicting value (Z=3.29,2.63,P＜0.01 or P＜0.05).

BACKGROUND: Umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) have been shown to lead to new tissue formation after homing and engrafting to the heart. But the safety of UCB-MSCs engrafting remains to be further investigated. OBJECTIVE: To study the safety and apoptosis inhibition of the UCB-MSCs under co-culture conditions on human cardiomyocytes. METHODS: UCB was collected at delivery with informed consent obtained from 10 donors. The UCB-MSCs were treated with 5-azaserine to induce differentiation into cardiomyocytes. The in vitro cultured cells of the 3rd-5~(th) passages and dividing cells were taken to detect telomerase activity, tumor-related gene expression, G-banding patterns of chromosomal karyotupes, cell surface antigen expression, tumor formation in nude mice, and inhibited apoptosis under co-culture conditions. RESULTS AND CONCLUSION: Prior to and after 5-azaserine induction, telomerase activity and tumor-related gene expression (p53, cyclin A, cdk2, β-actin, C-fos, h-TERT, c-myc) of UCB-MSCs were similar, no abnormal chromosomal karyotupes were observed, immunophenotype exhibited no change, CD34 was negative, but CD44 and CD90 (Thy-1) were positive. At 10 weeks after inoculation of UCB-MSCs, nude mice still survived healthily and no formed tumor in vivo was observed. Hematoxylin-eosin staining suggested normal subcutaneous tissue. Compared with simple cardiomyocytes, UCB-MSCs could significantly inhibit cardiomyocyte apoptosis under co-culture conditions (P < 0.05), indicating that human UCB-MSCs are a valuable, safe, and effective source of cell transplantation treatment.

Objective To study the safety and effect of the umbilical cord blood(UCB)-derived mesenchymal stem cells(MSCs)on apoptosis of human cardiomyocytes(HCM). MethodsUCB was collected at the time of delivery with informed consent obtained from 10donors.The UCB-derived MSCs were treated with 5-azaserube(5-AZA)and were further induced to differentiate into cardiomyocytes.Telomerase activity,G-banding patterns of chromosomal karyotypes,tumor formation in nude mice,RT-PCR,and the effect of inhibiting apoptosis of HCM were investigated. ResultsMSCs derived from UCB were differentiated into cardiomyocytes in vitro,which possessed telomerase activity after 5-AZA induction,and no abnormal chromosomal karyotypes were observed.Expression of p53,cyclin A,cdk2,β-actin,C-fos,h-TERT and c-myc were similar in MSCs before and after 5-AZA treatment.There was no tumor formation in nude mice after injection of UCB-derived MSCs.UCB-derived MSCs significantly inhibited apoptosis of HCM. ConclusionUCB-derived MSCs are a valuable,safe and effective source of cell-transplantation treatment.

Six patients with ST segment elevated acute myocardial infarction (AMI), who were 52.5 years old in average, were enrolled and performed the treatment at Tongren Hospital from November 2003 to June 2004. Following percutanecus transluminal coronary angioplasty and stent revascularization, autologous bone marrow stem cell (BMSC) transplantation was performed after informed consent was obtained. Patients were subcutaneously injected with granulocyte colony-stimulating factor (G-CSF) at 1 week before transplantation. When CD34+ cells going up to 1%-3% in peripheral blood, mononuclear cells in peripheral blood were harvested,purified, and further infused into the infarcted related coronary artery with an over-the-wire balloon catheter. Following up was performed every half a year. Four years later, the infarcted area of these patients was further decreased by 8.03%, in the basic descent of 42.7% at 3 months averagely; total infracted area descent was 50.73%, but ejection fraction increased by 4.6% from 50.8%. There was no serious coronary artery restenosis and/or stenosis formation which need revascularization upon angiography.