Objective To study the protective effect of lipoxin A4(LXA4)against sepsis-induced acute kidney in-jury(SAKI)in rats and its effect on the expression of toll-like receptor 4(TLR4),myeloid differentiation factor88(MyD88),nuclear factor kappa B(NF-κB)in the kidney.Methods Forty male specific pathogen-free C57BL/6J mice were randomly divided into SAKI group,SAKI+LXA4 group,sham procedure group,sham procedure+LXA4 group.The mice in SAKI group and SAKI+LXA4 group were given cecum ligation and puncture(CLP)to establish SAKI animal models.The mice in SAKI+LXA4 group and sham procedure+LXA4 group were given LXA4(40 ng/kg)with intraperitoneal injection 30 mins after CLP.All mice were sacrificed at the 24th hour after CLP to collect serum,urine and kidney tissues.The enzyme linked immunosorbent assay(ELISA)was used to test the serum creat-inine(Scr),blood urea nitrogen(Bun),interleukin-1 β(IL-1β),IL-6,tumor necrosis factor-α(TNF-α)and urine neutrophil gelatinase-associated lipocalin(NGAL),kidney injury molecule 1(KIM-1)levels of mice.The pathologi-cal changes were examined through hematoxylin-eosin(HE)staining and Periodic Acid-Schiff(PAS)staining.And the mRNA levels of TLR4,MyD88,NF-κB p65 were detected through real-time PCR(RT-PCR);the expression lev-els of TLR4,MyD88,NF-κB p65,phospho-NF-KB p65(p-NF-KB p65)were detected through immunohistochemistry(IHC)and Western blot assay.Results ELISA showed that the values of Scr,Bun,IL-1 β,IL-6,TNF-α,NGAL,KIM-1 in SAKI group were higher than those in SAKI+LXA4 group(P＜0.05),and there were no significant differences in Scr,Bun,IL-1 β,IL-6,TNF-α,NGAL,KIM-1 between sham procedure group and sham procedure+LXA4 group.HE and PAS staining showed that SAKI group had severer pathological changes than SAKI+LXA4 group in the kidney structure(P＜0.05),while pathological structures of the kidney were normal in sham proce-dure group and sham procedure+LXA4 group.RT-PCR showed that the mRNA levels of TLR4,MyD88,N F-κB p65 in SAKI group,SAKI+LXA4 group were higher than sham procedure group and sham procedure+LXA4 group(P＜0.05);the mRNA levels of TLR4,MyD88,NF-κB p65 in SAKI group were higher than SAKI+LXA4 group(P＜0.05);The results of IHC,Western blot assay were as follows:The expression levels of TLR4,MyD88,NF-κB p65,p-NF-κB p65 in SAKI group,SAKI+LXA4 group were higher than sham procedure group and sham procedure+LXA4 group(P＜0.05);the expression levels of TLR4,MyD88,NF-κB p65,p-NF-κB p65 in SAKI group were higher than SAKI+LXA4 group(P＜0.05).Conclusion TLR4/MyD88/NF-KB pathway plays an important role in the occurrence and development of SAKI,and LXA4 may reduce SAKI by inhibiting TLR4/MyD88/NF-κB sig-naling pathway.

Objective To explore the distributions of dose differences and their correlations with tumor types in independent three-dimensional dose verification for intensity-modulated radiotherapy(IMRT)plan,and to establish prediction models.Methods The fixed field IMRT plans of 180 patients with head and neck,chest,and abdominal tumors were collected.Independent sample t-test was used to analyze the consistency between the initial dose calculated in treatment planning system and the verified dose for evaluating the feasibility of independent validation.The distributions of planning target volume(PTV)dose differences among different tumor types were analyzed.The correlations of PTV dose differences with conformity index(CI)and homogeneity index were analyzed using correlation analysis and multiple linear regression method.Results The PTV dose differences for head and neck,chest,and abdominal tumors were within±1.2%,and the average 3%/3 mm gamma passing rate between the initial dose and the verified dose was higher than 99.5%,indicating good consistency in dose distribution between independent validation and treatment planning system.The distributions of PTV dose differences were different in head and neck,chest,and abdominal plans,and were significantly correlated with CI which could be used to preliminarily determine the PTV dose differences in independent validation.The multiple regression equation based on CI and homogeneity index could be used to estimate the dose verification differences of IMRT plan.Conclusion Independent verification can quickly achieve pre-treatment validation for radiotherapy plans,improving the efficiency of quality control of radiotherapy plans.The estimation based on dose differences provides guidance for optimizing radiotherapy plans.

Purpose To investigate the expression status of GNL3 in gastric cancer,and to explore the role of GNL3 in tumor proliferation,invasion and migration.Methods The ex-pression of GNL3 mRNA in gastric cancer tissues was analyzed by searching database.The expression of GNL3 protein in 51 gastric cancer tissues and 51 adjacent non-tumor tissues was de-tected by immunohistochemistry(IHC)SP method.The correla-tion between GNL3 protein expression and gastric cancer clinical pathological features was analyzed by x2 test.The expression of GNL3 in gastric cancer cells was silenced by transfection of sh-RNA,and the silencing efficiency was verified by Western blot.The effect of silencing GNL3 on the proliferation of gastric cancer cells was examined by CCK-8,colony formation and EdU stai-ning.In addition,wound-healing assay and Transwell assay were performed to detect the effect of GNL3 silencing on cell invasion and migration.Results SangerBox and UALCAN database re-trieval showed that the expression of GNL3 mRNA was signifi-cantly increased in gastric cancer tissues(P＜0.01).IHC stai-ning showed that the positive expression rate of GNL3 protein in gastric cancer tissues was 96.1％,and the high expression rate was 78.4％,which was significantly higher than that in adjacent non-tumor tissues(74.5％,51.0％,P＜0.01).Moreover,the high expression of GNL3 was significantly correlated with lymph node metastasis in gastric cancer patients(x2=4.933,P=0.026).CCK-8,colony formation and EdU staining showed that GNL3 silencing inhibited the proliferation of gastric cancer cell SGC-7901.The wound-healing and Transwell assay showed that GNL3 silencing inhibited the migration and invasion of gastric cancer cell.Conclusion The GNL3 protein is highly expressed in gastric cancer tissues,and closely related to the proliferation,migration and invasion of gastric cancer cells.

Objective:To explore the relationship between social exclusion, rumination, executive function and mobile phone addiction among college students.Methods:From November to December 2023, a total of 516 college students were investigated by social exclusion questionnaire for undergraduate, ruminative responses scale, the Geurten-questionnaire of executive functioning in Chinese college students and mobile phone addiction tendency scale. SPSS 26.0 statistical software was used for common method bias test, descriptive statistics, correlation analysis, and PROCESS 3.5 macro program was used to test the mediation effect.Results:Social exclusion (31.21±12.69), rumination (42.85±12.38), executive function (71.46±9.41), and college students' mobile phone addiction tendency (43.53±11.74) were all significantly and positively correlated with each other ( r=0.299-0.500, all P<0.01). The direct effect of social exclusion on mobile phone addiction was significant (effect size=0.138, 95% CI=0.048-0.228), accounting for 37.91%(0.138/0.364) of the total effect. Rumination had a mediating effect between social exclusion and mobile phone addiction (effect size=0.053, 95% CI=0.001-0.112), accounting for 14.56%(0.053/0.364) of the total effect. Executive function mediates the relationship between social exclusion and mobile phone addiction (effect size=0.137, 95% CI=0.091-0.188), accounting for 37.64%(0.137/0.364) of the total effect.Rumination and executive function has a chain mediating effect between social exclusion and mobile phone addiction (effect size=0.036, 95% CI=0.016-0.061), accounting for 9.89%(0.036/0.364) of the total effect. Conclusion:Social exclusion can directly affect mobile phone addiction in college students and can also influence mobile phone addiction in college students through the independent mediating effects of rumination and executive function, as well as the chain mediating effect of rumination and executive function.

Objective To establish a multi-component quantitative control method for Shenhuang Yangyin Capsules;To explore the application of chemical pattern recognition and grey relational analysis(GRA)model in the quality evaluation of Shenhuang Yangyin Capsules.Methods The contents of ginsenoside Rg1,ginsenoside Rb1,ginsenoside Rb3,ginsenoside Rd,calycosin 7-O-β-D-glucopyranoside,ononin,calycosin,medicarpin,sodium danshensu,salvianolic acid B,tanshinone ⅡA,methylophiopogonanone A and methylophiopogonanone B in Shenhuang Yangyin Capsules were determined by HPLC.Agilent Eclipse Plus C18 column was adopted with acetonitrile as mobile phase A and 0.5％phosphate acid solution as mobile phase B,gradient elution.The detection wavelengths were 203,280 and 296 nm.Through chemical pattern recognition analysis and GRA,the results of multi-component content were analyzed,and the comprehensive quality evaluation system of Shenhuang Yangyin Capsules was constructed.Results The results of methodological verification of external standard method met the requirements.Chemometric analysis showed that salvianolic acid B,ononin,tanshinone ⅡA,calycosin,ginsenoside Rb3 and ginsenoside Rb1 were the main factors affecting the quality of Shenhuang Yangyin Capsules.The relative correlation degree of GRA were 0.319 8-0.642 1,and it was helpful for enterprises to analyze the differences between products.Conclusion Multi-component quantitative combined chemical mode recognition and GRA can be used for quality evaluation of Shenhuang Yangyin Capsules.

Objective:To explore the mediating roles of rumination and avoidance coping between information anxiety and suicidal ideation.Methods:In March 2022, a total of 896 college students were surveyed by the information anxiety scale(IAS), ruminative responses scale(RRS), coping style questionnaire(CSQ)and self-rating idea of suicidal ideation scale(SIOSS). The ANOVA analysis, Pearson correlation analysis and mediating effect analysis were performed by the SPSS 22.0 software.Results:The detection rate of suicidal ideation among college students was 9.15%(82/896). The information anxiety score was(73.84±17.29), the rumination score was(47.73±12.16), the avoidance coping score was(3.76±2.52), and the suicidal ideation score was(5.41±4.09). Information anxiety was significantly positively correlated with rumination, avoidance coping and suicidal ideation( r=0.49, 0.36, 0.37, all P<0.05). Rumination was significantly positively correlated with avoidance coping and suicidal ideation( r=0.42, 0.59, both P<0.05). There was a significantly positive correlation between avoidance coping and suicidal ideation( r=0.45, P<0.05). Information anxiety affected suicidal ideation of college students through four paths.The direct effect value of information anxiety on suicidal ideation was 0.06, accounting for 16.67% of the total effect. The effect values of the separate mediating effect of rumination and avoid coping were 0.22 and 0.04, and accounting for 61.11% and 11.11% of the total effect respectively. The chain mediating effect value of rumination and avoid coping was 0.04, accounting for 11.11% of the total effect. Conclusion:Information anxiety can directly affect suicidal ideation of college students and indirectly affect suicidal ideation through rumination and avoidance coping.

Objective To explore the current situation,research hotspots and future development direction of medical community in our country.This paper puts forward some suggestions for the future development of medical community in our country,and provides the research reference.Methods A total of 1 329 articles on medical community published by CNKI from February 2016 to July 2016 were 2023 by NoteExpress and CiteSpace,from the point of view of medical service homogeneity,this paper analyzes the general situation,research hotspots and research trends of the medical community domain literature,this paper reviews the research from the perspectives of medical expense payment,medical personnel training and health file management.Results The research topics of the medical community are gradually diversified and characterized,the research methods of the medical community are diversified,and the research perspectives of the medical community are differentiated.Conclusion Fu-ture research should focus on the following aspects:the scope of medical community research topics,health records management,government support system,medical integration in the post-epidemic era.

Traditional Chinese Medicine (TCM) external therapy for sleep disorder of chronic fatigue syndrome (CFS) has good anti-fatigue effect and can improve sleep quality of patients. The treatment for sleep disorders of CFS with TCM external treatment mainly adopts acupuncture, moxibustion, massage, TCM bath, transcutaneous acupoint electrical stimulation and auricular point sticking, etc., or alone, or comprehensive application, or combined with oral Chinese materia medica. The appropriate treatment method can be selected according to the patients' condition and compliance, which reflects the unique advantages of TCM syndrome differentiation and treatment and the treatment according to people and time. The existing research still needs to further form a standardized and recognized diagnosis and treatment system, so as to better guide clinical popularization and application.

Abstract In recent years, the overall incidence of scoliosis in children and adolescents in China is on the rise, and it has become the third largest "killer" affecting the health of children and adolescents in China after obesity and myopia. The dysfunction caused by spinal deformity has no obvious pathological manifestations or clinical symptoms in children and adolescents (except for severe scoliosis). It is easy to be ignored in the early stage, but if it is not intervened in time, this will cause serious damage to the long term prognosis and quality of life of the patients. Therefore, through early scoliosis screening, we can effectively monitor the spinal health status of children and adolescents in China, ensure timely diagnosis of the disease, timely intervention (brace and other conservative treatment), and play the role of early prevention. Avoid further deterioration of scoliosis and surgical intervention. This article will focus on the research progress of screening and conservative treatment of scoliosis.

Objective:To study the association between preoperative hemoglobin amount and incidence of lower limb deep vein thrombosis (DVT) in patients with lower limb fracture.Methods:A retrospective study was performed of the 2, 482 patients with lower limb fracture who had been treated at Department of Orthopaedics Trauma, Honghui Hospital Affiliated to Xi'an Jiaotong University from July 2014 to August 2019. They were 1, 174 males and 1, 308 females with an age of (60.6±19.3) years. Recorded were the patients' age, gender, injury time, hemoglobin amount, D-dimer measurement, combined medical conditions, time and results of ultrasound vein examination on both lower extremities. According to the ultrasound results, the patients were divided into a thrombosis group and a thrombosis-free group. The 2 groups were compared in hemoglobin amount. Logistic regression was used to analyze the relationship between preoperative hemoglobin amount and incidence of lower limb DVT. The patients were divided into 5 groups according to the quintile of hemoglobin amount; the incidences of thrombosis were compared between the 5 groups.Results:The total incidence of DVT in this cohort was 29.53%(733/2, 482). The hemoglobin amount in the thrombosis group was (116.57±19.24) g/L, significantly lower than that in the thrombosis-free group (124.76±19.79) g/L ( P<0.05). The preoperative hemoglobin amount was a risk factor for incidence of DVT after a lower limb fracture ( OR=0.985, 95% CI: 0.980 to 0.990, P<0.001). As the quintile level of hemoglobin increased, the incidence of DVT showed a downward trend. In comparison of the group with the highest DVT incidence (40.58%) and the group with the lowest DVT incidence (17.27%), the risk increased by 2.386 times (95% CI: 1.718 to 3.315). Conclusions:The preoperative hemoglobin amount can affect the DVT incidence after a lower limb fracture, and a low hemoglobin amount may more likely lead to lower limb DVT.