Objective: Recent advances in epigenetic studies continue to reveal novel mechanisms of gene regulation and control, however little is known on the role of epigenetics in sensorineural hearing loss (SNHL) in humans. We aimed to investigate the methylation patterns of two regions, one in RB1 and another in GJB2 in Filipino patients with SNHL compared to hearing control individuals. Methods: We investigated an RB1 promoter region that was previously identified as differentially methylated in children with SNHL and lead exposure. Additionally, we investigated a sequence in an enhancer-like region within GJB2 that contains four CpGs in close proximity. Bisulfite conversion was performed on salivary DNA samples from 15 children with SNHL and 45 unrelated ethnically-matched individuals. We then performed methylation-specific real-time PCR analysis (qMSP) using TaqMan® probes to determine percentage methylation of the two regions. Results: Using qMSP, both our cases and controls had zero methylation at the targeted GJB2 and RB1 regions. Conclusion Our study showed no changes in methylation at the selected CpG regions in RB1 and GJB2 in the two comparison groups with or without SNHL. This may be due to a lack of environmental exposures to these target regions. Other epigenetic marks may be present around these regions as well as those of other HL-associated genes.
Hearing Loss ; Methylation

Objective. The purpose of this study is to identify the incidence rate of 'refer' result in neonates born to diabetic mothers and to determine the association of maternal diabetes and the initial 'refer' result. Methods. This was a retrospective cross-sectional study which included neonates who had hearing screening test using transient-evoked otoacoustic emissions test (TEOAE) on both ears at the Philippine General Hospital Ear unit during three weeks. We obtained the demographic characteristics, presence/absence of maternal diabetes, and OAE results. Results. Among the 150 neonates, ten were born to diabetic mothers, with an age range of 2-8 days old. Forty percent of neonates of diabetic mothers had an initial 'refer' result compared with 7.9% of nondiabetic mothers' neonates. After logistic regression analysis, there is a significant association between maternal diabetes and initial 'refer' result in OAE with a p-value <0.05. If the mother is diagnosed with diabetes (gestational/pre-gestational), the odds of having an initial 'refer' result in the hearing screening is 2x higher. The odds can range from 2-43 times. Conclusion. The incidence rate of an initial 'refer' result in neonates of diabetic mothers is 40%. There is a significant association between maternal diabetes and the initial 'refer' result in the OAE test.
Infant, Newborn ; Humans ; Mothers ; Diabetes, Gestational ; Hearing Loss ; Mass Screening ; Risk Factors ; Hearing

Objective: The objective of this study is to evaluate the newborn hearing screening program in The Medical City based on the Joint Committee on Infant Hearing (JCIH) 2007 Position Statement Quality Indicators. Methods: Study Design: Cross - Sectional Survey. Setting: Tertiary Private Hospital. Participants: All newborns who underwent newborn hearing screening in The Medical City for the year 2015. Results: Of 2,010 patients delivered in the hospital in year 2015, 1,986 (98.8%) were screened. Among the 59 babies with initial “refer” results, 15 (25.42%) “referred” a second time while 24 (40.68%) “passed” the rescreening. Twenty (33.89%) did not undergo rescreening (10 were classified as dropouts, while another 10 did not undergo rescreening for various reasons. Of those who “referred” during rescreening, only 9 (60%) had further evaluation done with ABR/ASSR. Among these, 4 (26.66%) had hearing loss and proceeded with the appropriate monitoring and management while 5 (33.33%) had normal hearing. Conclusion The current newborn hearing screening program in the Medical City was able to reach JCIH 2007 quality indicators for screening but not for confirmation of hearing Loss. All patients with hearing loss were managed with early rehabilitation.
Otoacoustic Emissions, Spontaneous ; Evoked Potentials, Auditory, Brain Stem

OBJECTIVES: This study aims to determine the maternal reactions and emotions towards an initial "refer" result in the newborn hearing screening test and to determine the proportion of mothers who fully comprehended the test results of the newborn hearing screening test.
METHODS: In this study, mothers of newborns with a "refer" result in the Universal Newborn Hearing Screening (UNHS) are given questionnaires which cover their understanding of the hearing screening test, the emotions they felt and attitude towards the results.
RESULTS: Our findings showed that the UNHS program protocol was properly understood by almost all the respondent mothers. Of the emotions listed from the questionnaire, being "worried" was the most persistently felt emotion.
CONCLUSION: It is important that the results of the newborn hearing screening test be properly understood by the mothers. Mothers who experienced negative emotions brought about by the hearing screening test still believed that the newborn hearing screening test was important, were glad that their child underwent the newborn hearing screening test and will request it for their future offspring.

Anxiety

OBJECTIVES: This study determined the initial otoacoustic (OAE) hearing screening results of newborns with collapsed ear canals and vernix caseosa in the ear canal and compared these to ears that were patent.
METHODS: Two hundred term newborns (400 ears) with normal APGAR scores, birth weight, maternal and gestational history, who were born between August 2013 to October 2013 and who had OAE hearing screening test done by trained midwives were included in this study. All of them underwent otoscopy after the OAE hearing screening test was done to determine patency of the ear canal and presence of vernix caseosa. The examining physician was blinded to the OAE results. Comparison between the OAE results and the otoscopic findings were done.
RESULTS: Four hundred ears were included in the study. Two hundred and fifty one ears (62.8%) had vernix caseosa and 42 ears (10.5%) had collapsed ear canal. The overall initial OAE hearing screening test pass rate of the newborns tested was 69.5%. The initial OAE hearing screening test pass rate of newborns those with ear canal vernix caseosa or collapsed ear canal, were 72.1% and 47.6%, respectively. Patent ears were found in 107 (26.7%) with a pass rate of 71.9%.
CONCLUSION:The pass rates of ears with vernix caseosa and collapsed ear canal were 72.1% and 47.6%, respectively. There was no significant difference between the OAE hearing screening test pass rates of ears with patent canal and ears that were collapsed and/ or had vernix caseosa. However, there was a statistically significant difference in pass rates between patent ear canals and collapsed ear canal

Otoscopy ; Infant, Newborn ; Vernix Caseosa

BACKGROUND: In 2000, the World Health Organization recommended implementation of universal hearing screening. The Philippines enacted this policy into law in 2009 as it was found to be cost-effective for the Philippines. The model at the time used a hospital-based approached to screening. This paper examines the cost-effectiveness and budget impact of implementing a community-based universal hearing screening program.
METHODS: A model was developed following a community-based hearing screening program. Parameters were obtained through literature review, secondary data analysis, and consultation with experts. Cost-effectiveness was assessed for a single birth cohort from a public payer and societal perspective.
RESULTS: A community-based universal hearing screening program was found to be cost-saving. One-way sensitivity analysis showed that results were sensitive only to treatment rate and follow-up rate. The program is also a high budget impact program.
CONCLUSION: A community-based hearing screening program is cost-saving for the Philippines. Ensuring treatment and good follow-up in testing will ensure cost-effectiveness.

Hearing Loss ; Cost-Effectiveness Analysis

OBJECTIVES: This paper aims to determine the length of time before a cochlear implantee would reach aided hearing threshold of < 45 dBHL at 5 frequencies (target threshold) and whether there is a difference depending on age of implantation and sex.
METHODS: From January 2009 to February 2011, the aided earing thresholds of 41 prelingually deaf children with cochlear implants after switch on were reviewed.
RESULTS: There were 21 males and 20 females. Majority of children 7 years old reached it at < 1 month. The probability that a female would reach the target threshold on or before 6 months was 90% but this was not statistically significant.
CONCLUSION: Sixty-six percent of the children with cochlear nplants reached the target threshold within the first 3 months post fitting and 80% within the first 6 months post fitting. More of the older children reached target threshold earlier than the younger children. There was a trend that more females reached the target threshold earlier than the males but this was not statistically significant.

Human ; Male ; Female ; Child ; Child Preschool ; Infant ; Infant Newborn ; Cochlear Implants ; Cochlear Implantation ; Hearing Aids ; Cochlea

OBJECTIVE: To explore the effects of pesticide exposure on the auditory system, specifically on hearing status based on auditory brainstem responses.

METHODS: A cohort of pregnant women was identified in several communities in a rural area from April 2002 to February 2003 and followed up until delivery. Mother-infant dyads were assessed for exposure to pesticides. Maternal and fetal exposures to environmental toxic products were determined by measuring levels in maternal hair and blood, and infant cord blood, hair, and meconium, respectively. Hearing status was measured using otoacoustic emissions (OAE) and confirmed by diagnostic auditory brainstem responses (ABR) measured at 80, 60, and 40 decibels. Waves I, III, V were identified and absolute latencies measured, including inter-peak latencies from waves 11III, I-V, and III-V. Pesticide exposure was then correlated with latencies of Waves I, III, V, and interpeak latencies of waves I-III, IIV, and III-V. Hearing loss and pesticide exposures were correlated with Griffiths Mental Development Scores (GMDS).

RESULTS: Significant delays in the ABR wave latencies were noted in the group with exposure to pesticides. Propoxur was the most common toxic product detected in infants and meconium the best substrate for its detection. There was a 1.4% risk of hearing loss with exposure to propoxur (RR=0.52 (0.12-2.30), p = 0.06), a 6.25% risk with cypermethrin exposure (RR= 4.53 (0.61133.64), P = 0.10) and 6.25% risk with pretilachlor exposure (3.13 (0.44-22.30), p = 0.07). Griffith's Mental Developmental Scale scores (GMDS- hearing and speech subscale and general quotient scores) were not significantly different between exposed and unexposed groups. However, three infants with positive exposures and hearing loss had below average, or low to average scores using this scale.

CONCLUSION: Maternal exposure to environmental toxic products may affect the auditory pathway in infants at birth. Pregnant women should limit their exposure to such toxic products in order to avoid neurodevelopmental effects particularly on hearing because this is very important in the critical stage of language and speech development.

Human ; Male ; Female ; Infant Newborn ; Auditory Pathways ; Maternal Exposure ; Meconium ; Speech ; Otoacoustic Emissions, Spontaneous ; Hearing Loss ; Deafness ; Hearing Tests ; Acetanilides ; Pesticides ; Hair

Objective:To report a case of fractured tracheotomy tube ingestion in a pediatric patient, discussing the clinical presentation, complications and management of tracheobronchial tree versus upper digestive tract foreign bodies. Methods: Design: Case Report Setting: Tertiary Private Hospital Patient: One Result: A 4-year-old male child with unusual hypersensitivity to routine tracheotomy suctioning was discovered to have a fractured tracheotomy tube. Emergency radiographs localized the cannula in the abdomen and bronchoscopy was deferred. The foreign body was eventually passed out after four days. Conclusion: Due diligence in diagnostics prior to bronchoscopy led to the avoidance of an unnecessary and sometimes complicated procedure. In developing countries with poor access to health care, the importance of regular tracheotomy follow-ups and periodic replacement cannot be overemphasized. A search of the English literature using Pubmed and Ovid search engines with keywords tracheostomy, foreign bodies and pediatrics confirms that this is the first reported accidental ingestion of a fractured tracheotomy tube in a pediatric patient.