Objective To study and compare the oral microbiota and metabolites of children with Henoch Schonlein purpura(HSP)to identify specific microbiota and metabolites related to this disease and elucidate the pathogenesis of HSP.Methods Three groups of qualified subjects were included,including 20 in the HSP group,20 in the HSP nephritis(HSPN)group,and 20 in the control group.Perform high-throughput 16S rRNA sequencing and metabolic profiling of saliva from each group to analyze the correlation between differential microbiota and differ-ential metabolites.Results(1)Compared with the control group,there was a significant difference in richness and diversity in the HSPN group(P＜0.05).At the same time,there was no significant difference in richness and diver-sity in the HSP group(P＞0.05).Compared with the HSP group,the abundance,and diversity of the HSPN group were significantly increased(P＜0.05).At the genus level,the proportion of Streptococcus in each group is the high-est.Compared with the control group,there was no significant correlation between the HSP group and the genus of bacteria.In contrast,the HSPN group showed a significant increase in the genera of Pseudomonas and Parabacteroi-des(P＜0.05).Compared with the HSP group,the abundance of Pseudomonas and Parabacteroides in the HSPN group was significantly increased(P＜0.05).(2)Compared with the control group,the HSPN group had 12 differen-tial metabolites involving nine metabolic pathways,such as phenylalanine metabolism;There was no significant dif-ference in metabolites and no metabolic pathway in the HSP group.Compared with the HSP group,the HSPN group has 15 differential metabolites involving nine metabolic pathways,such as phenylalanine metabolism.(3)In the HSPN and control groups,Pseudomonas and Parabacteroides negatively correlated with Phenylalanine metabolic pathway products.In the HSPN and HSP groups,Pseudomonas,Parabacteroides,and Phenylalanine metabolic path-way products were negatively correlated.The metabolites involved in phenylalanine metabolism in the oral cavity are 2-hydroxycinnamic acid,Phenylpyruvic acid,and N-acetyl-L-phenylalanine.Conclusion There is a significant dif-ference between HSPN and HSP children and healthy children.Streptococcus,Pseudomonas,and Parabacteroides may be one of the trigger factors of HSPN,and Phenylalanine metabolism may be one of the pathways in the patho-genesis of HSPN.Children with HSPN have a more pronounced imbalance in oral microbiota and greater differences in metabolic products than children with HSP.

Objective To standardize nursing management on postoperative patients with a left ventricular assist-ant device(LVAD).Methods The first draft of the Consensus was formed on the basis of literature review.2 rounds of expert consultations and a round of online meeting discussion were held for adjustments and modifications the draft of the Consensus.Results The recovery rate of the inquiry questionnaire was 93.75%.The authority coefficients of the 2 rounds of inquiry experts were 0.927 and 0.920.The concentration degree of expert opinions for each indicator was greater than 3.5 score,and the coefficient of variation was less than 0.25.The Kendall harmony coefficients for 2 rounds of correspondence were 0.402 and 0.407(P<0.01).The final Consensus formed through expert consultations and meetings includes 7 themes:hemodynamic monitoring,LVAD function monitoring,coagulation function monitoring,percutaneous cable and wound care,exercise rehabilitation care,health education and guidance,and pre-discharge assessment.Conclusion The Consensus is scientific,rigorous,and authoritative.The Consensus covers all aspects of postoperative care for patients with LVAD,and it will benefit to clinical practice.

Color is an important indicator for evaluating the ornamental traits of horticultural plants, and plant pigments is a key factor affecting the color phenotype of plants. Plant pigments and their metabolites play important roles in color formation of ornamental organs, regulation of plant growth and development, and response to adversity stress. It has therefore became a hot topic in the field of plant research. Virus-induced gene silencing (VIGS) is a vital genomics tool that specifically reduces host endogenous gene expression utilizing plant homology-dependent defense mechanisms. In addition, VIGS enables characterization of gene function by rapidly inducing the gene-silencing phenotypes in plants. It provides an efficient and feasible alternative for verifying gene function in plant species lacking genetic transformation systems. This paper reviews the current status of the application of VIGS technology in the biosynthesis, degradation and regulatory mechanisms of plant pigments. Moreover, this review discusses the potential and future prospects of VIGS technology in exploring the regulatory mechanisms of plant pigments, with the aim to further our understandings of the metabolic processes and regulatory mechanisms of different plant pigments as well as improving plant color traits.
Plant Viruses/genetics* ; Plants/genetics* ; Gene Silencing ; Plant Development ; Gene Expression Regulation, Plant ; Genetic Vectors

Objective:To optimize the clinical nursing pathway, service program and evaluation parameters of percutaneous coronary intervention(PCI), for references for the cost accounting and compensation mechanism of nursing program in public hospitals.Methods:After literature analysis and group discussion, the initial templates were constructed for the PCI clinical nursing pathway, nursing service projects, and their evaluation parameters. 15 experts were consulted by two rounds of Delphi method to optimize PCI nursing path, nursing service items and their evaluation parameters (basic labor consumption, basic time consumption, technical difficulty and risk degree).Results:Two rounds of Delphi method finally determined the PCI clinical nursing path and 27 nursing service items, and adjusted the evaluation parameters of 10 nursing service items. The new projects for PCI clinical nursing services included adjustment and review of dual antiplatelet therapy plans, postoperative rehabilitation nursing, and key project verification. The three nursing service projects with the highest level of technical difficulty and risk were intravenous blood transfusion, gastric catheterization, and gastrointestinal decompression. The two items with the highest importance assigned were high pump assisted arterial/venous infusion (blood) and invasive continuous arterial blood pressure monitoring.Conclusions:The PCI clinical nursing pathway and nursing service project constructed in this study could closely integrate with clinical practice, highlight the integrated nursing service model, and reflect the labor value of nurses.

Objective:To improve the early graded rehabilitation nursing model suitable for postoperative children with congenital heart disease, providing reference for related research and clinical practice.Methods:Searched databases like JBI, PubMed, Medline, CINAHL, CNKI, Wanfang Data and related websites for information on postoperative rehabilitation of children with congenital heart disease. We improved the first draft model with qualitative interview results and used Delphi method to conduct two rounds of consultation for 16 experts from 6 provinces and cities to further test the scientific and feasibility of the model.Results:The early graded rehabilitation nursing model for postoperative children with congenital heart disease includes 4 first-level items, 15 second-level items and 48 third-level items. The 4 first-level items are the evaluation of the early graded rehabilitation nursing model, the grading standard of the early graded rehabilitation nursing model, the implementation of the early graded rehabilitation nursing model, the effect evaluation and health education. Experts′ response rates were 100% in the 2 rounds, experts′ authority coefficient were 0.82 and 0.84 respectively, and the Kendall′ s W rank-order correlation coefficients of all levels of indicators were 0.188-0.246, 0.223-0.287 (all P<0.01). Conclusions:The improved early graded rehabilitation nursing model for postoperative children with congenital heart disease is scientific, pertinence and safe, which can provide guidance for clinical rehabilitation nursing practice.

Cardiac rehabilitation can effectively prevent and control the occurrence and evolution of cardiovascular diseases. Nurses play an important role as direct caregivers for patients and executors of specific tasks. Cardiac rehabilitation nursing led by specialized nurses can provide patients with professional guidance and improve rehabilitation outcomes. This article summarizes the research progress of cardiac rehabilitation nursing led by specialized nurses within and outside the hospital, as well as in the new form. Based on the current situation in China, this article also proposes thoughts and suggestions to provide reference for the development of cardiac rehabilitation nursing in China.

Children with congenital heart disease often face pain after surgery. Uncontrollable pain can cause a series of physiological and psychological reactions in children, affecting their prognosis. Therefore, timely adoption of scientific and standardized pain management is particularly important for children. This article summarizes pain assessment methods, pain relief measures, and pain management models for children with congenital heart disease, so as to provide reference for standardized pain management after surgery for children with congenital heart disease in China.

ObjectiveTo observe the clinical effect of Tianma Goutengyin combined with acupuncture on adult patients with tic disorder of ascendant liver-yang hyperactivity. MethodThe 64 adult patients with tic disorder of ascendant liver-yang hyperactivity who were treated in the outpatients and inpatients of the 989^th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army from January 2019 to December 2020 were selected and classified into the control group （32 cases） and the treatment group （32 cases） with the random number table method. The control group took oral western medicine Duloxetine Hydrochloride Enteric Capsules， and the treatment group was treated by Tianma Goutengyin and acupuncture. The treatment lasted 8 weeks for both groups and the efficacy was observed. ResultThe traditional Chinese medicine （TCM） syndrome score， Yale Global Tic Severity Scale （YGTSS） score， total bile acid （TBA）， triglyceride （TG）， and partial pressure of carbon dioxide （PaCO₂） were significantly lower， and the Na⁺ concentration and partial pressure of oxygen （PaO₂） were significantly higher than those before treatment in both groups （P<0.05）. After treatment， the TCM syndrome score， YGTSS score， and TBA were significantly lower， and and the Na⁺ concentration and PaO₂ were significantly higher than in the treatment group than in the control group （P<0.05）. The efficacy on TG and PaCO₂ was similar in the two groups. The total clinical effective rate of the treatment group was 90.6% （29/32）， as compared with the 64.5% （20/31） of the control group （χ²=6.210， P<0.05）. The incidence of clinical adverse reactions in the treatment group was 12.5% （4/32） in comparison with the 35.5% （11/31） in the control group （χ²=4.585， P<0.05）. The recurrence rate in the treatment group （9.4%， 3/32） was lower than that （32.3%， 10/31） in the control group （χ²=5.035， P<0.05）. ConclusionThe combination of Tianma Goutengyin and acupuncture can remarkably alleviate the clinical symptoms of adult tic disorder patients with ascendant hyperactivity of liver yang， reduce TBA， TG and PaCO₂， and increase the concentration of Na⁺ and PaO₂. Patients treated with the combination show small adverse reactions and low recurrence rate.

Objective To investigate the feasibility of using a sitting aldosterone renin ratio (ARR) instead of a standing ARR to screen for primary aldosteronism (PA) in hypertensive patients. Methods From April to July 2017, 63 hypertensive patients admitted to the Department of Hypertension of Fuwai Hospital of Chinese Academy of Medical Sciences were selected as subjects by convenient sampling. During the hospitalization, the patients performed two position tests on different days. On the first day, the patients were asked to keep the standing and walking position for 2 hours, and the standing blood samples were taken. The next day, the patients were asked to keep the sitting position for 2 hours and then take the blood samples. Comparing ARR results for both body position measurements. Results A total of 62 patients successfully completed the study. There were no significant differences in peripheral blood ARR results between the sitting and standing position of patients with same age groups and genders (P＞ 0.05). Conclusions The sitting position ARR does not affect the PA test results. It is recommended to use the sitting position ARR for PA screening in clinical practice,which can help reduce the incidence of adverse reactions and interruption events.

Objective To investigate the effect of atorvastatin on blood lipid and carotid atherosclerosis in patients with hyperlipidemia and cerebral infarction.Methods 98 patients with hyperlipidemia and cerebral in-farction in our hospital from September 2014 to February 2017 were selected and divided into observation group(49 cases)and control group(49 cases)according to the random number table method.The control group was treated with conventional treatment,the observation group was treated with atorvastatin on the ba-sis of the control group.The two groups were treated for 4 weeks.The ADL score,NIHSS score,blood lipid index,carotid IM T and carotid plaque area were compared between the two groups before and after treatment. Results ADL score of the patients in the observation group after treatment higher was than the control group (t=12.7783,P<0.05),NIHSS score was lower than the control group(t=10.941,P<0.05);LDL-C,TC and TG levels after treatment in the observation group were than in the control group(t= 17.239,9.216, 9.800,P<0.05),while the HDL-C level was higher than the control group(t=7.683.P<0.05);the patients in the observation group were treated by carotid artery IM T and carotid plaque area was higher than that of the control group(t=8.919,6.344,P<0.05).Conclusion Atorvastatin can improve hyperlipidemia in pa-tients with cerebral infarction and carotid atherosclerosis,dyslipidemia,has the important research signifi-cance.