1.Effect of siRNAs on HSV-1 Plaque Formation and Relative Expression Levels of RR mRNA
Zhe REN ; Shen LI ; Qiaoli WANG ; Yangfei XIANG ; Yunxia CUI ; Yifei WANG ; Renbin QI ; Daxiang LU ; Shumin ZHANG ; Peizhuo ZHANG
Virologica Sinica 2011;26(1):40-46
RNA interference(RNAi)is a process by which introduced small interfering RNA(siRNA)can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1(HSV-1)RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
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