Regional odontodysplasia (ROD) is a rare localized dental developmental anomaly. The typical clinical manifestations of ROD are abnormal tooth eruption, abnormal development of enamel and dentin. The radiographic characteristic is "ghost teeth". Its etiology still remains unknown. The care and treatment of a patient with ROD needs a multidisciplinary approach. And the treatment should be taken after the assessment of each individual case of ROD. This paper reviews the definition, etiology, epidemiological features, clinical manifestations, imaging features, dental microstructure and treatment strategies of ROD to provide reference for clinical diagnosis and treatment.

Objective:To analysis the mid-stage prognosis of transapical aortic valve implantation(TA-TAVI) using J-Valve? system for the treatment of high-risk aortic regurgitation(AR) patients.Methods:Data of 25 patients with aortic regurgitation who had underwent transapical aortic valve implantation using J-Valve? system were collected in the Second Affiliated Hospital of Medical College of ZheJiang University from September 2016 to June 2020 . Analysis and summarize their postoperative all-cause mortality, the incidence of adverse events and the improvement in cardiac function.Results:There were 25 patients, including 19 males, the age rage from 59-83 years, the average age was(72.3±27.11) years. The levels of aortic regurgitation was evaluated by transthoracic echocardiography preoperatively, showed that severe AR accounted for 88%. The New York Heart Association(NYHA) of grade 3 or above was 92%. The most common comorbidity was hypertension, accounted for 68%. Coronary heart disease and history of cardiac surgery was 5 and 3 relatively in this study. The Society of Thoracic Surgeons score before surgery was 1.511%-27.674%, the average of STS score was 4.27(2.914-6.033)%. Successful J-Valve implantation was obtained in all 25 cases, no conversion to thoracotomy. After surgery, 2 patients required permanent pacemaker implantation, 1 patient needed continuous renal replacement therapy(CRRT) due to acute kidney injury, 1 occurred moderate or above paravalvular leak. The results showed good therapeutic effects in early-stage, low incidences of adverse events. The continued improvement of cardiac function and ventricular reverse remodeling could be observed in mid-stage.Conclusion:In this study, we can summarize that high-risk patients with aortic regurgitation treated with transapical aortic valve implantation using J-Valve? system can acquire great perioperative safety and mid-stage prognosis.

Objective:To investigate the clinical effect of the bell-bottom flap in the treatment of multiple little toes with syndactyly deformity of the 4th and 5th toes.Methods:Forty cases (22 males and 18 females) of multiple little toes with syndactyly deformity of the 4th and 5th toes were treated in the Plastic Surgery Department of Fujian Maternal and Child Health Hospital from June 2014 to May 2020. The age of patients ranged from 6 months to 36 months, with an average of 11 months. There were 46 feet, among which 19 cases had a complete fusion, and 27 cases had an incomplete fusion. During the operation, the polydactyly was removed, the fibula-deviation deformity of little toes was corrected with the articular surface formation and joint capsule repaired. The bell-bottom flap was used to reconstruct the webbed of the toe, and the wound surface on the side was closed with three flaps made up of the residual skin soft tissue of multiple little toes. Meanwhile, the soft tissue length at the distal end of the little toe was extended, and the toenail fold and palmar flexion transverse lines were reconstructed to form a similar appearance to the normal.Results:All the flaps in all 46 feet survived with no flap necrosis, skin grafting, or infection. After 6 to 36 months of follow-up (averagely, 20 months), the width, depth, and slope of the reconstructed toe webbed were normal or close to normal, the fibula deviation of the small toe was corrected entirely, the deformity of the short toe was improved in varying degrees, no toe scar contracture, and the appearance of nail fold was consistent with that of the normal small toe.Conclusions:Bell-bottom flap is a good method to correct the deformities of small toe polysyndactyly, which can reconstruct the well-formed webbed, no skin grafting, with the shape of small toenail fold and small toe naturally, and the radians of palmar flexion line naturally.

Objective:To investigate the clinical effect of the bell-bottom flap in the treatment of multiple little toes with syndactyly deformity of the 4th and 5th toes.Methods:Forty cases (22 males and 18 females) of multiple little toes with syndactyly deformity of the 4th and 5th toes were treated in the Plastic Surgery Department of Fujian Maternal and Child Health Hospital from June 2014 to May 2020. The age of patients ranged from 6 months to 36 months, with an average of 11 months. There were 46 feet, among which 19 cases had a complete fusion, and 27 cases had an incomplete fusion. During the operation, the polydactyly was removed, the fibula-deviation deformity of little toes was corrected with the articular surface formation and joint capsule repaired. The bell-bottom flap was used to reconstruct the webbed of the toe, and the wound surface on the side was closed with three flaps made up of the residual skin soft tissue of multiple little toes. Meanwhile, the soft tissue length at the distal end of the little toe was extended, and the toenail fold and palmar flexion transverse lines were reconstructed to form a similar appearance to the normal.Results:All the flaps in all 46 feet survived with no flap necrosis, skin grafting, or infection. After 6 to 36 months of follow-up (averagely, 20 months), the width, depth, and slope of the reconstructed toe webbed were normal or close to normal, the fibula deviation of the small toe was corrected entirely, the deformity of the short toe was improved in varying degrees, no toe scar contracture, and the appearance of nail fold was consistent with that of the normal small toe.Conclusions:Bell-bottom flap is a good method to correct the deformities of small toe polysyndactyly, which can reconstruct the well-formed webbed, no skin grafting, with the shape of small toenail fold and small toe naturally, and the radians of palmar flexion line naturally.

Objective:To explore the evaluation of transesophageal echocardiography(TEE) in patients with massive regurgitation of posterior mitral valve prolapse undergoing transapical off-pump NeoChord repair.Methods:Eight patients from April to July 2019 in the Second Affilliated Hospital of Zhejiang Univerity with massive regurgitation of posterior mitral valve prolapse underwent NeoChord repair mitral valve morphology, prolapse position and regurgitation degree were evaluated before NeoChord implantation by TEE. Under TEE guidance, the puncture site was identificated, the position and length of artificial chordae were adjusted during implantation. NeoChord′s function and positon after implantation were observed. The complications were monitored during the operation.Results:Mitral valve repair by NeoChord system was successfully performed with implantation of 2 to 4 artificial chordaes in eight patients respectively. Intraoperative TEE and pre-discharge transthoracic echocardiography(TTE) showed moderate MR in two patients, mild to moderate MR in one patient, mild MR in the remaining five patients. Reexaminations with TTE at 1 month after operation showed moderate MR in six patients, and mild to moderate MR in two patients. And no postoperative complications were noted.Conclusions:NeoChord system is a safe, effective and feasible treatment method for patient with mitral valve prolapse, TEE plays an important role during NeoChord implantation.

Objective: The purpose of this study was to explore the relationship between maternal tea consumption and birth outcomes. Methods: From January 2005 to December 2010, pregnant women were recruited from Nanjing Maternity and Child Health Hospital. The basic information and the situation of tea consumption during pregnancy were investigated using questionnaire and the birth outcomes of newborns were followed up. Finally, 500 pairs of mothers and infants with complete and standard-compliant data were included in the analysis. The differences of birth outcomes between the tea consumption group and the non tea consumption group were compared and the associations between tea consumption and birth outcomes were analyzed using multivariate logistic regression. Results: Mother's tea consumption rate was 32.8% (164 cases) during pregnancy. The rate of low birth weight in the tea consumption group was (5.5%, 9 cases) and higher than that in the non-tea consumption group (2.1%, 7 cases) (P=0.042). There was no significant difference in the rate of premature birth, small for gestational age, fetal distress, and macrosomia between the two groups After the adjustment of maternal age, education level, family income, weight gain during pregnancy, pre-pregnancy BMI, parity, gestational hypertension, gestational diabetes, neonatal gender and gestational age, compared with non-tea consumption group, there was a positive effect on low birth weight, OR(95%CI) was 4.76 (1.06-21.48). The OR (95%CI) value of the low birth weight risk of the tea group was 5.30 (1.04-26.92) compared with the non-tea consumption group after the adjustment of additional factors such as passive smoking, coffee consumption, folic acid supplement, mineral supplement, carbonated beverage consumption. Simultaneously, compared with non-tea consumption group, there was no statistically significant association between tea consumption during pregnancy and premature birth, small for gestational age, fetal distress and macrosomia (P>0.05). Conclusion Tea consumption during pregnancy was a risk factor for low birth weight in offspring.

Arginine kinase (AK) is a key enzyme in energy metabolism of invertebrates and plays an important regulatory role in the life activities such as growth and development, nutrition utilization, immune resistance and stress response. Arginine kinase of Bombyx mori (BmAK) is related to the energy balance and anti-NPV process, but there is little research on its molecular structure and enzymatic properties. We cloned the ORF sequence of BmAK gene, and analyzed chromosomal localization, genomic structure, mRNA structure, secondary and tertiary structure. Phylogenetic analysis indicated that AK was highly conserved in evolution. Soluble recombinant BmAK was obtained by prokaryotic expression, and purified by Ni-NTA affinity chromatography. The circular dichroism spectroscopy showed that BmAK contained α-helix structures, and its α-helix structures were relatively stable in the pH range between 5 and 10. Enzyme activity analysis showed that the optimum temperature of BmAK was 30 ℃ and the optimum pH of BmAK was 7.5. The optimal temperature of BmAK was 25 ℃. Between 15 ℃ and 30 ℃, the structure and activity of BmAK was relatively stable. The structure of BmAK was relatively stable at pH 7.0. Our findings reveal the structure and function of BmAK to develop novel green safe and environmentally friendly insecticides.

Objective To analyze the correlation of family function with parenting self-efficacy (PSE) in autism spectrum disorder (ASD) family. Methods Cohort study was carried out. From August, 2015 to February, 2016, caregivers of ASD children (n=142) and healthy children (n=131) were enrolled into investigation group (n=173) and control group (n=131). They were assessed with Family Assessment Device (FAD) and Tool to Measure Parenting Self-efficacy (TOPSE). Results There were significant differences in the scores of FAD and TOPSE between two groups (P<0.05). The scores of FAD were lower in the high income, urban ASD families than in the low income, rural ASD families (P<0.05). The scores of FAD negatively correlated with the scores of TOPSE in the ASD families (r=-0.152 to -0.257, P<0.05). Conclusion The family function and level of PSE of the ASD families was obviously inferior to the healthy families, and the worse the family function is, the lower the level of PSE will be. Individualized and family-centered intervention strategies should be developed to provide a good family environment for the rehabilitation of children with ASD.

Objective To evaluate clinical efficacy and safety of fluvoxamine in the treatment of trichotillomania.Methods 24 outpatients who met the DSM-Ⅳ diagnosis criteria for trichotillomania received fluvoxamine for 12 weeks and 17 tricholillomania outpatients weren't treated with fluvoxamine.Clinical efficacies and adverse reactions were assessed with the spence children's anxiety scale(SCAS),Kovacs children's depression inventory (CDI),clinical global impression(CGI) and treatnent emergent symptom scale(TESS) before treatment and the end of the 4th and 12th week.Results After 4 weeks treatment,the scores of the SCAS (59.75 ± 12.35),CDI (24.95±7.68) and CGI(SI) (3.79±0.93) were lower than those before treatment(71.50±20.65,31.60± 10.40,5.88±0.68 respectively) (P＜0.05).After 12 weeks treatment the scores of the SCAS,CDI and CGI (SI) were lower than those after 4 weeks treatment (P＜0.05).After 12 weeks treatment the scores of the CGI(GI) (1.54±0.59) were lower than that at the end of 4 weeks treatment (2.96± 0.69) (P＜ 0.05).Conclusion Fluvoxamine has a good efficacy and higher safety in the treatment of trichotillomania.

Aortic Valve Insufficiency ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; Humans ; Transcatheter Aortic Valve Replacement