Introduction: The ability of a person to self-regulate and practise healthy lifestyle behaviours determine one’s weight status. The objective of this study was to determine the relationship of self-regulation, dietary practices, and physical activity with overweight status among male and female adolescents. Methods: This crosssectional study used multistage cluster sampling involving government secondary school students aged between 13 and 14 years old in Hulu Langat, Selangor. Students answered a validated self-administered questionnaire comprising socio-demography, dietary practices, physical activity, and self-regulation items. Their body mass index (BMI) was calculated, and weight status was determined using the Centers for Disease Control and Prevention (CDC) BMI chart. Results: Among 636 students, 27.0% were “overweight”, affecting more male than female students (p=0.032). Majority of them (96.7%) were motivated to maintain healthy body weight, but only a third of the students took vegetables, fruits and grains (32.4%), and performed vigorous physical activity (31.1%) regularly. Regardless of the “overweight” status, there were no significant differences in dietary practices, physical activity, and autonomous regulation for both genders. Female students showed a higher level of controlled regulation than male students in non-overweight (p=0.005) and overweight (p<0.001) groups. Higher controlled regulation increased the odds of being overweight among female students (AOR=1.04, 95% CI=1.04-1.08, p=0.010). Conclusion: Thus, health authorities need to develop programmes to assist, particularly female students, in practising higher autonomous regulation to combat overweight and obesity, as this group exhibits a high level of controlled regulation, which increases the likelihood of being overweight.