1.Antifungal properties of Mimusops elengiseeds against paddy seed-borne fungi and selected pathogenic fungi 152-158
Sze-Chi LEE ; Syahidah Akmal MUHAMMAD ; Mahamad Hakimi IBRAHIM ; Nik Mohd Izham Mohamed Nor
Malaysian Journal of Microbiology 2018;14(2):152-158
		                        		
		                        			
		                        			Aims:The  ever-revolving  fungi  strains  and  environmental  and  health  concerns  due  to  current  practice  of  synthetic pesticide in agricultural fields have encourages more ventures into bio-pesticides research. Mimusops elengi, a widely available endogenous plant intropical countries and most parts of this plant have been proven to possess medicinal and antimicrobial  potential.  In  this  study, M.elengiseeds  crude  extracts  are  tested  for  their  antifungal  activities  on  paddy seed-borne and pathogenic fungi.Methodology  and  results:The  dried  and  grinded M.elengiseeds  are  macerated  separately  using  water,  methanol, ethyl acetate, dichloromethane and petroleum ether as extraction medium. Crude extract of each solvent wasused on paddy  seed  surface  treatment  to  determine  their  antifungal  inhibition  potential  on  seed-borne  fungi  and  paddy  grain germination.  Synthetic  fungicide  mancozeb  and  thiram  are  also  tested  as  comparisons  to  the  performance  of  plant extracts. Water andmethanol extracts exerted the best fungal inhibiting and grain germination results from the five crude extracts  tested   and   qualitative   phytochemical  screening   reveals   both   extracts  contained   the   most   number   of phytoconstituents including saponin, flavonoids,  alkaloids, tannins and phenolic.  Water extract, methanol extracts  and synthetic  fungicides  are  then  subjected  to  in-vitro  bioassay  to  observe  their  effect  on  mycelial  growth  of  several  fungi strains  pathogenic  to  paddy  namely, Fusarium  fujikuroi,Curvularia  aeria,C.lunata andC.eragrostidis.Water  and methanol  extracts  showed  a  very  similar  trend  of  inhibition  on  all  four  fungi  strains  tested  with  best  percentage  of inhibition on mycelia growth of C.eragrostidisfollowed by C. aeria, C. lunataand least effective on F.fujikuroi. Further separation  of  crude  extract  need  to  be  done  to  isolate  the  specific  acting  compounds  contributing  to  fungal  growth inhibition.Conclusion,  significance  and  impact  of study:Both  water  and  methanol  extracts  of  the  seeds  contain  promising antifungal properties on seed borne fungi which is as good as the synthetic fungicides compared in this study. A broad range of active phytochemical properties it possesses may be the contributing factor for the fungal growth inhibition. This preliminary screening could narrow down the potential of this seed extracts as natural antifungal agents and the acting active compounds.
		                        		
		                        		
		                        		
		                        	
            
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