Objective To study the expression and roles of miR-31-5p in acute myeloid leukemia (AML).Methods miR-31-5p in AML patients were evaluated by real-time PCR;THP-1 cells were transfected with the miR-31-5p mimic and control, respectively.The effects of over-expression of miR-31-5p were examined by CCK-8 and FACS analysis;Dual-luciferase and Western blot were performed to detect target gene HuR expression.The effects of knock-down of HUR were also examined by CCK-8 and FACS analysis.Results miR-31-5p was down-regulated in AML patients compared to the normal control.Over-expression of miR-31-5p in THP-1 cells reduces cell proliferation and accelerates monocytic differentiation.miR-31-5p could target HuR, and knock-down of HuR inhibits cell proliferation and attenuates monocytic differentiation in THP-1 cells.Conclusions miR-31-5p may regulate AML cell proliferation and monocytic differentiation by targeting HuR.

Objective To study the developmental patterns and aging characteristics of anterior intervertebral disc and spinal canal based on anatomy CT scan and three dimensional reconstruction imaging measurement in the adolescents ages from 12 ~18 years old,and to provide the anatomical basis for the treatment and prevention of disc herniation diseases.Methods A total of 30 adolescents,who were with normal spines and without any the problems of nervous system,were selected to do CT scan of lumbosacral vertebrae through dual-source 64-slice spiral CT.CT original data of lumbosacral,which were translated into DICOMformat,were measured and analyzed statistically with Mim-ics software.Results There was no significant difference among the intervertebral disc and the superior sagittal diameter of the spinal canal, which were not influenced by gender (P >0.05).The intervertebral disc and the superior sagittal diameter of the spinal canal would increase with age with wavy trends,and there were some significant differences(P <0.05).Conclusion The anatomy parameters of lumbosacral disc and spinal changed regularly.In order to achieve satisfactory results of the lumbosacral disease treatment,it must be combined with individual radiographic findings,biomechanics and materials science,etc.

Objective To study the developmental patterns and aging characteristics of anterior sacral foramina and posterior sacral forami-na in adolescents aging from 12 to 18 years old through CT imaging scanning and three-dimensional reconstruction measurement,and to provide the anatomical basis for the diagnosis and treatment of sacral vertebra diseases.Methods The 30 adolescents without any the problems of nerv-ous system were selected to conduct 64-slice spiral CT in the pelvic cavity.The original data were input into three-dimensional reconstruction software Mimics with the form of DICOM,and the relevant indicators were measured and analyzed.Results There was no significant difference in the height,width and distance of inner edge of anterior sacral foramina and the posterior sacral foramina in different gender and sides (P >0.05).The physical development of human increases gradually with age and there were significant differences.Conclusion There is a regular change process in the relevant indicators of anterior sacral foramina and posterior sacral foramina in adolescents.But the clinical appli-cation must be combined with the results of individual imaging,the technology of reverse engineering and the support of rapid prototype manu-facturing so as to meet the satisfactory results of individualized treatment.

Objective To study the developmental patterns and aging characteristics of lumbar pedicle morphology in the adolescents aging from 12~18 years old,and to provide the anatomical basis for image and three-dimensional analysis of the treatment and prevention of spinal diseases. Methods the 30 adolescents with normal spines and without any the problems of nervous system were selected to do thin spiral CT scan that the range was lumbar. The original data imported into three-dimensional reconstruction software with the form of DICOM were to measure and analyze statistically the relevant indicators according to gender and age. Results There was no significant difference in the pedicle height,pedicle width and length of form screw entry point to vertebral anterior cotrex,which were not influenced by age and gender (P>0. 05). The physical development of human increases gradually with age and has some significant differences. Conclusion A regular change process is displayed to provide references for clinic. But it must be combined with the results of individual imaging and the technology of reverse engineering and the support of rapid prototype manufacturing. Only in this way can it meet the satisfaction of individualized treatment.

Objective To study the pedicle of lumbosacral developmental patterns and aging characteristics by means of studying the ado-lescents aging from 12~18 years old,and provide the basis for image and three-dimensional analysis of the treatment of spinal diseases. Meth-ods Select the 30 normal spines of adolescents without any the problems of nervous system,who did thin spiral CT scan of lumbosacral verte-brae. The original data in the form of DICOM were put into three-dimensional software to do the relevant measurement and analyzed according to gender and age. Results There were no significant differences between the pedicle of lumbosacral E/F angle and DSP /DNP and these differ-ences were not influenced by age and gender(P>0. 05). The physical development of human increases gradually with age and has some signifi-cant differences. Conclusion A regular change process and operation in the region value in patients with lumbarsacrum is displayed. But it must be combined with the results of individual imagine and the technology of reverse engineering and the support of rapid prototype manufactur-ing. Only in this way can it meet the satisfaction of individualized treatment.

Objective To examine the expression of neuron-specific enolase (NSE) in patients with remission diffuse large B-cell lymphoma (DLBCL),and to explone its prognostic value.Methods Serum NSE values were mesured by electmchemilumineseence immunoassay in 20 health donors and 40 DLBCL patients reached remission after 6-8 cycles of chemotherapy after initial diagnosis.Results Positive expressions rate of NSE in remission DLBCL patients was 50 ％,and was 10 ％ in health donors (P ＜ 0.01).In remission DLBCL patients,there was significant difference in the 2-year PFS rate between in NSE-positive group and negative group (6.6 ％ vs 20.9％,P ＜ 0.001).Serum NSE was an independent poor prognostic factor in remission DLBCL patients.Conclusion Serum NSE expressions is an independent poor prognostic factor of remission DLBCL and has significant clinical value.

Objective: To evaluate the knowledge, attitude and behavior on blood lipid among people in Changsha and to provide evidences for prevention and control of blood lipid abnormality. Methods: A total of 400 cases were randomly selected on the questionnaire of the knowledge, attitude and behavior on blood lipid in ordinary adults who participate in health examination in Xiangya Hospital. Blood lipid related physical examination was conducted at the same time. hTe health examination participants were divided into several groups according to their sex, age, degree of education, marriage and family income. The influential factors for knowledge, attitude and behavior were analyzed. Results: hTe knowledge score of blood lipid for health examination participants was 18.33±8.67 (total score 37), the attitude score was 6.63±2.45 (total score 9) and the behavior score was 8.32±2.65 (total score 16). hTe scores of female was higher than that of male in the terms of knowledge and behavior (bothP<0.05); the scores in the 40–49 age group were lower than those in the other age groups (all P<0.05); the scores in the junior high school group were lower than those in the other education groups (allP<0.05); the scores in the family group with less than 2 000 yuan income were lower than those in other family groups with different income (allP<0.05). The multiple stepwise regression analysis showed that: 1) the knowledge on blood lipid score was inlfuenced by ages and education background (bothP<0.05); 2) while the attitude of blood lipid was inlfuenced by four factors such as education background, systemic blood pressure, blood sugar and triglyceride(allP<0.05); 3) the behavior on blood lipid was inlfuenced by ifve factors such education background, triglyceride, systemic blood pressure, blood sugar and ages (allP<0.05). Conclusion: The knowledge, attitude and behavior on blood lipid among health examination participants were mostly influenced by education background, gender and ages. Thus, clinical medical staff should prevent the blood lipid abnormality through the health education and improve the knowledge in normal people. hTe group of 40–49 age male should be thought as the primary intervention subjects. hTe knowledge, attitude and behavior on blood lipid among the general population is also related to individual’s blood pressure, blood sugar and triglyceride. So the clinical medical staff should also improve the knowledge of blood lipid, blood pressure and blood sugar in general population for improving their attitude and unhealthy habits. In addition, the active control of blood sugar and blood pressure can enhance the overall health status of the general population.

Neuron specific enolase (NSE) is a glycolytic enzyne,which is localized specifically to the neuronal cytoplasm and has been proposed as a biochemical marker of neuronal cell damage.This article summarizes research on NSE in blood diseases from the present literatures.

OBJECTIVE: To determine the clinical effect of standardized training and management of peripherally inserted central catheter (PICC) and catheter-related complications. METHODS: A total of 610 patients were divided into a control group and an observation group, the control group (n=300) were catheterized by trainees who received "short-term intensive training", the observation group (n=310) by "system standardized training and management". The clinical efficacy of catheterization and the rate of catheter-related complications were compared. RESULTS: There was significant difference in the one-time puncture success rate, one-time cannulation success rate, the time for operation and the pain score between the 2 groups (all P<0.01), and there was also significant difference in the occurrence of catheter extrusion, plug, arrhythmia, catheter-related thrombosis, phlebitis, puncture point effusion and catheter-related infection between the 2 groups (all P<0.05). CONCLUSION Standardized PICC training and management can improve the effect of catheterization and reduce the incidence of PICC-related complication.
Catheter-Related Infections ; prevention & control ; Catheterization, Peripheral ; methods ; Humans ; Incidence ; Inservice Training ; Thrombosis

The peripherally inserted central catheter occlusion is not rare in adults.Its occurence is related with the catheter's tip position,catheter type and the skills of medical staff.Personnel training is particularly important in the prevention of occlusion.Urokinase and heparin can be used for thrombolysis.