Objective To observe the changes in serum a proliferation inducing ligand(a proliferation inducing ligand,APRIL)and N-myc downstream regulated gene 1(N-myc downstream regulated gene 1,NDRG1)levels,and analyze their diagnostic value for ovarian endometrioma(OEM).Methods From July 2021 to July 2022,132 patients with OEM who visited Zigong First People's Hospital were regarded as the observation group,and regular follow-up was conducted.According to the prognosis of these patients,they were grouped into the recurrence group(n=50)and the non recurrence group(n=82).Meanwhile,78 healthy individuals who had their medical checkups at the hospital during the same period were the control group.Enzyme linked immunosorbent assay(ELISA)was applied to detect serum APRIL and NDRG1 levels,and the general data of the recurrent and non recurrent groups were compared.Logistic regression analysis was applied to analyze the relevant factors affecting the prognosis of OEM.Pearson analysis was applied to explore the correlation between serum APRIL and NDRG1 levels in patients with OEM.Receiver operating characteristic(ROC)curve was applied to evaluate the diagnostic value of serum APRIL,NDRG1 levels and their combination for OEM.Results Compared with the control group,APRIL level(35.28±6.81ng/ml vs 26.37±3.19ng/ml)and NDRG1 level(124.39±15.67μg/L vs 9.67±10.82μg/L)in observation group were increased,and the differences were significant(t=10.864,17.278,all P<0.05).Compared with the non recurrence group,the serum levels of APRIL(40.38±7.88ng/ml vs 32.16±6.18ng/ml)and NDRG1(132.04±19.83μg/L vs 119.73±13.16μg/L)in the recurrence group were increased,and the differences were significant(t=6.668,4.287,all P<0.05).Logistic regression analysis showed that serum APRIL and NDRG1 levels were risk factors for the prognosis of patients with OEM(Waldχ2=11.839,28.437,all P＜0.001).Pearson method analysis results showed a positive correlation between serum APRIL level and NDRG1 level in patients with OEM(r=0.439,P<0.001).The area under the curve(AUC)of combined diagnosis of serum APRIL and NDRG1 levels in patients with OEM was 0.849,with a sensitivity and specificity of 73.95%and 85.37%,respectively,which was better than the single prediction of APRIL and NDRG1(Z =2.644,2.094,P=0.008,0.036).Conclusion The serum levels of APRIL and NDRG1 were increased in patients with OEM.The combination of the two has high clinical value in the diagnosis of OEM,which may be closely related to the prognosis of patients with OEM.

Chondroma/surgery* ; Humans ; Liver ; Soft Tissue Neoplasms/surgery*

The emergence of SARS-CoV-2 variants of concern and repeated outbreaks of coronavirus epidemics in the past two decades emphasize the need for next-generation pan-coronaviral therapeutics. Drugging the multi-functional papain-like protease (PLpro) domain of the viral nsp3 holds promise. However, none of the known coronavirus PLpro inhibitors has been shown to be in vivo active. Herein, we screened a structurally diverse library of 50,080 compounds for potential coronavirus PLpro inhibitors and identified a noncovalent lead inhibitor F0213 that has broad-spectrum anti-coronaviral activity, including against the Sarbecoviruses (SARS-CoV-1 and SARS-CoV-2), Merbecovirus (MERS-CoV), as well as the Alphacoronavirus (hCoV-229E and hCoV-OC43). Importantly, F0213 confers protection in both SARS-CoV-2-infected hamsters and MERS-CoV-infected human DPP4-knockin mice. F0213 possesses a dual therapeutic functionality that suppresses coronavirus replication via blocking viral polyprotein cleavage, as well as promoting antiviral immunity by antagonizing the PLpro deubiquitinase activity. Despite the significant difference of substrate recognition, mode of inhibition studies suggest that F0213 is a competitive inhibitor against SARS2-PLpro via binding with the 157K amino acid residue, whereas an allosteric inhibitor of MERS-PLpro interacting with its 271E position. Our proof-of-concept findings demonstrated that PLpro is a valid target for the development of broad-spectrum anti-coronavirus agents. The orally administered F0213 may serve as a promising lead compound for combating the ongoing COVID-19 pandemic and future coronavirus outbreaks.
Animals ; Coronavirus Papain-Like Proteases/antagonists & inhibitors* ; Cricetinae ; Humans ; Mice ; Pandemics ; SARS-CoV-2/enzymology* ; COVID-19 Drug Treatment

Objective:To explore the knowledge of pediatric nurses about intravenous infusion therapy, find the problems, and provide a reference for managers to provide targeted training.Methods:In this multicenter cross-sectional study, the nurses on duty were selected from 43 hospitals under the medical alliance of Beijing Children's Hospital by convenient sampling from July 24th to 31st, 2018. The self-made questionnaires were used in this study, and data were collected online through wjx.cn. The questionnaires were developed by 4 veteran venous infusion therapists according to the Infusion Therapy Standards of Practice (2016 ) as well as the Technical Standards for Intravenous Therapy Nursing Issued by the National Health Commission of the People's Republic of China in 2014. The questionnaire mainly included three parts. Part I was the basic information of the respondent subjects and hospitals; Part II was the knowledge of the subjects about domestic standards for infusion therapy and Infusion Therapy Standards of Practice; and Part III the knowledge of the subjects about infusion therapy operations and maintenance as well as the current status of infusion therapy in their departments. SPSS 17.0 was used for statistical analysis. Results:A total of 8 405 pediatric nurses were investigated in this study. The average knowledge of infusion therapy was (11.72±2.41), and the pass rate was 54.8%. The survey showed that nurses performed well in selecting infusion tools and puncture sites according to the nature of the drug, evaluating the patient and treatment plan before infusion therapy, the sealing method of peripheral vein indwelling needles, and treatment methods when resistance was encountered or no blood was returned in suction. The univariate analysis revealed that the level of the hospital, whether the hospital had a dosing center and an infusion therapy group, the nurse's education, job title, working years, position, and whether to teach young nurses were the main influencing factors to nurses' knowledge about infusion therapy ( P<0.05) . The results of Logistic regression analysis showed that whether the hospital had a dosing center and a infusion therapy group, and the nurse's education background, title, working years, position, and teaching were the main influencing factors for the nurse's knowledge of infusion therapy ( P<0.05) . Conclusions:Pediatric nurses have insufficient knowledge about infusion therapy. They need to be further trained in the basics of infusion therapy, especially in the correct use of infusion tools, the proper selection of puncture sites, and catheter maintenance.

Objective:To understand the current situation of aggression and violence management attitudes of pediatric nurses in Class Ⅲ Grade A hospitals, and analyze its influencing factors.Methods:This research was a survey research. From September to December 2019, convenience sampling was used to select 110 pediatric nurses from three ClassⅢ Grade A hospitals in Wenzhou for investigation with the Management of Aggression and Violence Attitude Scale (MAVAS) and the Self -Transcendence Scale (STS) . A total of 110 questionnaires were distributed and 104 valid questionnaires were collected, with the effective recovery rate of 96.36%. Results:Among 104 pediatric nurses, the total scores of STS and MAVAS were (40.47±9.83) and (57.67±8.24) respectively. Univariate analysis showed that there were statistically significant differences in the MAVAS total score of pediatric nurses with different years of nursing work, whether they participated in relevant training, and whether they experienced violence ( P<0.05) . Regression analysis showed that years of nursing work, whether they experienced violence, and the level of self -transcendence were the influencing factors of pediatric nurses' aggression and violence management attitudes ( P<0.05) . Conclusions:The aggression and violence management attitudes of pediatric nurses in Class Ⅲ Grade A hospitals need to be improved. Years of nursing work, whether they experienced violence and the level of self-transcendence were the main influencing factors. Nursing managers can pay more attention to junior nurses who have not experienced violence so as to improve their aggression and violence management attitudes by improving the self -transcendence of this group.

Objective: To investigate the genetic characteristics of drug resistance and homology of extended-spectrum β-lactamase (ESBLs)-producing Klebsiella pneumoniae (Kpn) in hospitalized children in the pediatric intensive care unit (PICU) in Guiyang area. Methods: The Kpn strains were collected from hospitalized children at 3 PICU from the Affiliated Hospital of Guizhou Medical University, Guizhou Provincial People′s Hospital and Guiyang Children′s Hospital from September 2014 to December 2016.Automatic bacteria identification instrument and Kirby-Bauer method were used to identify Kpn strains and confirm ESBLs phenotype respectively.The drug resistance genes were detected by using polymerase chain reaction (PCR), and their homology was analyzed by means of pulsed field gel electrophoresis (PFGE) and cluster analysis. Results: (1) A total of 207 non-repetitive Kpn strains were isolated, of which 128 strains produced ESBLs (61.8%). There was no significant difference in the ESBLs detection rates among the different hospitals, years and types of samples(χ²=0.40, 5.19, 4.68, all P>0.05). (2) Among those 128 ESBLs-producing Kpn strains, 123 strains (96.1%) were found to have drug-resistant genes, of which the detection rate of SHV type was the highest, accounting for 87.5%.These 3 drug resistance genotypes were distributed in 7 modes, of which 85.2% were carrying more than 2 genotypes.(3) PFGE genotypic assay showed that there were 109 genotypes and 5 dominant clones.The PICU at 3 hospitals were all prevalent with type A strain, accounting for 14.8%.In 2015, there was a short-term outbreak of type A strain in Guiyang Children′s Hospital.While, the resistance phenotypes of Kpn and the genotype of PFGE could both be consistent or different. Conclusions The ESBLs-producing Kpn at PICU in Guiyang area has the characteristics of multi-drug resistance, its drug-resistant genotypes have diversified, and there is a small-scale short-term outbreak epidemic, which poses a serious threat to clinical anti-infection treatment.It is necessary to strengthen clinical drug resistance monitoring and adopt effective infection control measures to prevent and control the spread of nosocomial infections.

Objective? To investigate the status of pediatric nurses' social skills and perceived professional benefits and the relationship in between. Methods? A total of 400 pediatric nurses from four ClassⅢGrade A hospitals in Zhejiang province were recruited in this study from February to July 2018. Self-designed general information questionnaire, Scale of Social Skill for Nursing, and Nurses' Perceived Professional Benefits Scale were used to investigate the pediatric nurses. A total of 400 questionnaires were distributed and 385 valid questionnaires were returned, with an effective recovery rate of 96.25%. Results? The average score of Scale of Social Skill for Nursing was (162.89±22.57); the average score of Nurses' Perceived Professional Benefits Scale was (127.12±15.74); the total Scale of Social Skill for Nursing and it's dimensional scores were positively correlated with the total scores of Perceived Professional Benefits Scale and its dimensions (P＜ 0.05). Hierarchical regression analysis showed that job title, monthly income, social skills, physical contact and respect for patients were influencing factors of professional benefit of pediatric nurses (P＜0.01). Conclusions? The social skill level of pediatric nurses has an impact on their sense of occupational benefit. Nursing administrators can improve nurses' social skills to increase perceived professional benefits, thereby reducing the willingness to leave and stabilizing the nursing work teams.

Objective To evaluate the safety and efficacy of percutaneous balloon pulmonary valvuloplasty (PBPV)in the treatment of the children with pulmonary stenosis (PS),and to observe the long - term prognosis and analyze the influencing factors. Methods The total of 230 children were collected,who had been diagnosed with pul-monary valve stenosis and had undergone percutaneous balloon pulmonary valvuloplasty between November 1987 and November 2015 in Shandong Provincial Hospital Affiliated to Shandong University. Their ages ranged from 4 months to 17 years,and the follow - up duration lasted from 1 month to 29 years. The data included clinical data and long - term follow - up data of hospitalized children,and the echocardiography data from the healthy peers in the same period. Then the data were analyzed statistically. Results In this study,228 cases of children were successfully performed PBPV, and the success rate was 99％(228 / 230 cases). The pulmonary transvalvular gradient (△P)of preoperation,24 hours postoperatively,half a year postoperatively,2 years postoperatively,5 years postoperatively,and 10 years postope-ratively was (63. 5 ± 23. 8)mmHg (1 mmHg = 0. 133 kPa),(26. 2 ± 11. 1)mmHg,(24. 8 ± 9. 8)mmHg,(20. 9 ± 8. 9)mmHg,(18. 1 ± 8. 7)mmHg,(15. 3 ± 7. 3)mmHg and (15. 3 ± 7. 3)mmHg,respectively. The immediate post-operative △P was significantly lower than that of preoperation (P < 0. 01),and the △P of the most children decreased in the long - term follow - up. The results of Logistic regression analysis showed that valve dysplasia with right ventricu-lar outflow tract stenosis and the immediate postoperative residual transvalvular gradient degree were the risk factors for long term curative effect of PBPV in children who could not reach the best standard. The restenosis rate was 4. 6％(3 /65 cases)with children followed up for more than 10 years. The incidence of long - term follow - up pulmonary valve regurgitation (83％)was significantly higher than that before operation (58％)and short term (68％)after operation, and the degree of regurgitation also increased (P < 0. 05),while the degree of regurgitation of the tricuspid regurgitation decreased gradually during the follow - up (P < 0. 05);the right ventricular diastolic diameter of the patients at 10 years or more after the operation was measured as (19. 27 ± 3. 03)mm,which was significantly higher than that (15. 24 ± 2. 89)mm of the healthy children of at the same term healthy age (P < 0. 05). Conclusions The PBPV has a high success rate in the treatment of children with PS,and it has good medium - long - term curative effect,less com-plications and lower restenosis rate. Therefore,PBPV can be used as the first choice for PS. However,the incidence and degree of pulmonary regurgitation has an increasing trend after PBPV and the right ventricular diastolic diameter is still larger than that of the healthy children. Therefore,the long - term follow - up is necessary out of the hospital.

Objective To explore the application and effects of the cognitive evaluation theory-based family communication pattern in follow-up management in young stroke patients. Methods Totally 88 young patients with initial stroke admitted in the 2nd Affiliated Hospital of Wenzhou Medical University between June 2016 and June 2017 were selected, divided into the control group (n=44) and the intervention group (n=44) by governor-controlled study. Patients in the control group received conventional follow-up after discharge, while patients in the intervention group received follow-up management from the aspects of self-actualization needs, ability needs and belongingness needs based on the family communication pattern. The Self-management Behavior Scale for young stroke patients and Caregiver Burden Inventory (CBI) were used to compare the self-management behavior and caregiver burden between the two groups before and after the intervention. Results Six months after discharge, the intervention group's self-management behavior score was (73.69±11.65), while that of the control group was (66.37±16.78),the difference was statistically significant (t=2.377, P＜0.05). The caregiver burden score of the intervention group was (35.97±7.49), lower than that of the control group,the difference was statistically significant (t=3.866,P＜0.01). Conclusions The cognitive evaluation theory-based family communication pattern can effectively enhance the sense of coherence and reduce the caregiver burden in young stroke patients.

Objective To explore the status quo of effort-reward imbalance (ERI) and career success in clinical nurses and to analyze the correlation between the two.Methods Totally 737 clinical nurses from 2 ClassⅢ Grade A hospitals in Wenzhou of Zhejiang Province were selected by convenient sampling and investigated with the Chinese ERI scale and Career Success Scale (CSS) from December 2017 to February 2018.The relationship between ERI and career success in the clinical nurses was analyzed.A total of 737 questionnaires were distributed and 671 valid questionnaires were collected.Results The ERI ratio of the 671 clinical nurses was (1.09±0.31),and their total score of CSS was (33.92±7.64).ERI was found in 423 nurses (63.0%),and 457 nurses were overburdened (68.1%).There were statistical differences in CSS scores between the balanced nurses and the imbalanced as well as between the overburdened and the non-overburdened (P＜0.05).According to the Pearson correlation analysis,ERI was negatively correlated with career success in clinical nurses (P＜0.05).Conclusions Severe ERI is found in clinical nurses of Class Ⅲ Grade A hospitals,which affects their career success.It is advised that nursing managers take targeted measures to improve the status quo of ERI,complete career development planning for nurses,and enhance the working enthusiasm and career success in nurses.