Objective:To evaluate the efficacy and safety of endoscopic submucosal dissection (ESD) for circular superficial esophageal cancer.Methods:A retrospective analysis was conducted on 74 consecutive cases of circular superficial esophageal squamous cell carcinoma treated with ESD at Nanjing Drum Tower Hospital from January 2015 to December 2019. The success rate of ESD, curative resection rate, incidence of complications, and additional treatment were mainly observed.Results:One case was transferred to surgery, and the remaining 73 cases successfully completed ESD treatment. The success rate of ESD was 98.6%. Postoperative pathology of ESD revealed that 39 cases achieved curative resection, with a curative resection rate of 53.4% (39/73). Intraoperative muscle layer injury occurred in 15 cases (20.5%), and intraoperative perforation occurred in 1 case (1.4%). Two cases (2.7%) experienced delayed bleeding, and one case (1.4%) experienced delayed perforation. Eleven cases were lost to follow-up, and the remaining 62 cases received follow-up for 36.4±19.0 months. Among the follow-up cases, 12 underwent additional surgery and 5 cases additional chemotherapy and radiotherapy. Among the 57 patients with follow-up data who did not underwent surgery, 49 developed esophageal stenosis after ESD, with an incidence rate of 86.0%.Conclusion:ESD for circular superficial esophageal cancer is generally safe, but it is prone to muscle layer injury during the operation, with a low curative resection rate, a high incidence of postoperative esophageal stenosis, and a high proportion of additional surgical procedures.

There are still multiple challenges in China during the malaria post-elimination phase, including a large number of imported malaria cases with widespread distribution, low awareness of timely healthcare seeking, insufficient malaria diagnosis and treatment capacity of medical institutions and insufficient malaria surveillance and response capability of disease control and prevention institutions. As the core technical institutions for preventing the re-establishment of malaria transmission, both medical institutions and disease control and prevention institutions are required to enhance the collaboration between clinical and public health services, improve the malaria diagnosis and quality management system, intensify case identification and epidemiological investigations, and improve the management mechanism of antimalarial drug reserves. In addition, doctors are encouraged to become the main force in the health education and promotion of malaria prevention to improve the public health literacy. These approaches are recommended to improve the overall capability of timely identification, standardized treatment and effective response of imported malaria cases, so as to continuously consolidate the malaria elimination achievements in China.

Objective　To analyze the characteristics of the malaria epidemic and the indicators for "1-3-7" from 2021 to 2023 in China, understand the effectiveness and challenges in preventing re-establishment malaria in China, propose response strategies, and provide references for consolidating the achievements of malaria elimination. Methods　The individual malaria case data and focus data from 2021 to 2023 in the "Information System for Infectious Disease Surveillance" and the "Information System for Parasitic Diseases Prevention and Control" were collected, and epidemiological characteristics and indicators for “1-3-7” were analyzed. Results　From 2021 to 2023, a total of 4 132 malaria cases were reported in 31 provinces and Xinjiang Production and Construction Corps, with mainly falciparum malaria (59.2%, 2 445/4 132). The national malaria epidemic reached a historical low of 799 cases in 2021, before rebounding significantly to 2 488 cases in 2023. The top five provinces for malaria cases were Yunnan, Guangdong, Henan, Sichuan, and Shandong, accounting for 49.4% of the total (2 043/4 132) cases. Except for two long incubation cases infected with P. malariae and one non-mosquito-transmitted case, the remaining cases were imported from abroad, mainly from African countries (81.7%, 3 374/4 129), with P. vivax malaria mainly coming from Myanmar (63.7%, 638/1 001). Malaria cases mainly occur in middle-aged men and migrant overseas workers. 142 severe cases and 21 deaths of malaria were reported. 81.8% (3 378/4 132) of malaria cases sought medical attention within 3 days of symptom onset, and the initial diagnosis institutions were mainly county-level, municipal, and provincial hospitals(77.2%), with an accuracy malaria diagnosis rate of above 80.0%. The completion rates of the malaria indicators for "1-3-7" were all above 90.0%. Conclusions　Since the malaria elimination in China, there has been no re-establishment of malaria, and the surveillance response capability has been maintained at a high level. However, the epidemic of imported malaria continued to rise, severely endangering public health in China, especially in areas such as the China-Myanmar border where the risk of re-establishment was high. Currently, it is necessary to further enhance the awareness of the key populations about timely medical consultation for malaria, as well as healthcare workers' vigilance, diagnostic capabilities, and awareness of timely referrals. Efforts should be maintained to investigate and manage epidemics, strengthen prevention and control in key areas such as the China-Myanmar border in Yunnan, and continuously consolidate elimination achievements.

Humans ; Abdomen ; Dantrolene ; East Asian People ; Malignant Hyperthermia

Objective:To explore the curative effects of Nice knot fixation on tuberosity healing in hemiarthroplasty for complex proximal humeral fractures.Methods:A retrospective analysis was conducted of the eligible 32 complex proximal humeral fractures which had been treated at Department of Trauma and Orthopedics, Peking University People's Hospital between May 1, 2016 and May 1, 2019. Nice knot fixation was used to repair greater and lesser tuberosities in hemiarthroplasty for all the patients. There were 6 males and 26 females, aged from 60 to 90 years (mean, 74.9 years). By the Neer classification, there were 4 three-part fractures combined with dislocation, 20 four-part fractures, and 8 four-part fractures combined with dislocation. Shoulder joint X-rays were taken at postoperative 1, 2, 3, 6, and 12 months at the outpatient clinic to evaluate the patients' shoulder joint mobility, visual analog scale (VAS) pain score and Constant-Murley shoulder score. Tuberosity healing was assessed based on the X-rays and related complications were recorded.Results:The 32 patients received complete follow-up for 12 to 25 months (average, 17.82 months). At the 12-month follow-up, their shoulder flexion averaged 131.3° (from 80° to 155°), abduction 126.9° (from 80° to 155°), external rotation 48.4° (from 30° to 60°), internal rotation the L2 level, VAS pain score 0.9 (from 0 to 5), and Constant-Murley score 83.4 (from 58 to 96). The rate of patient satisfaction was 87.5%(28/32). Tuberosity-related complications were observed in 6 cases with an incidence of 18.8%. Complications like infection and prosthetic loosening were found in none of the patients.Conclusion:In hemiarthroplasty for complex proximal humeral fractures, application of Nice knot to fixate greater and lesser tuberosities can lead to rigid fixation, definite curative effects and a low incidence of tuberosity-related complications.

Objective:To investigate the clinical effect of Banxia Baizhu Tianma decoction combined with bean embedding in ear points on patients with cervical vertigo.Methods:From May 2018 to October 2020, 160 cases of cervical vertigo patients with phlegm turbidity and moderate resistance in Jinhua Central Hospital were selected as the research objects. According to the random number table method, the research objects were divided into ear acupoint buried beans group (A group, n=53), Banxia Baizhu Tianma decoction group (B group, n=54), Banxia Baizhu Tianma decoction combined with ear-buried beans group (C group, n=53). The three groups were treated with ear point burying bean, Banxia Baizhu Tianma decoction and Banxia Baizhu Tianma decoction combined with ear point burying bean for 10 consecutive days. The clinical effect, traditional Chinese medicine (TCM) syndrome score, cerebral blood flow velocity and hemorheology of the three groups were compared. Results:There was no significant difference in the total clinical effective rate between group B and group C ( P>0.05). The total clinical effective rate of group C was significantly higher than that of group A, the difference was statistically significant ( P<0.05). After treatment, scores of vertigo, neck and shoulder pain, headache and symptoms of daily life and work in 3 groups were significantly higher than before treatment, the differences were statistically significant (all P<0.05); The scores of TCM syndrome in group C were significantly higher than those in group A and group B, with statistical significance (all P<0.05). After treatment, the cerebral blood flow velocity of left vertebral artery (LVA), right vertebral artery (PVA), and basilar artery (BA) in the three groups were significantly increased compared with that before treatment, and the cerebral blood flow velocity of LVA, PVA, and BA in group C was significantly faster than that in A, B group, with statistical significance ( P<0.05). After treatment, the plasma viscosity, whole blood high shear viscosity and hematocrit of the patients in the three groups were significantly lower than those before treatment, and the cerebral blood flow velocity of group C was significantly lower than that of group A and group B ( P<0.05). Conclusions:Banxia Baizhu Tianma decoction combined with ear acupoint buried beans in the treatment of cervical vertigo with middle resistance of phlegm and turbidity can improve the symptoms of cervical vertigo, cerebral blood flow velocity and reduce blood viscosity, which is worthy of clinical promotion.

Objective:To investigate the metabolic disorder of gut microbiota and short-chain fatty acids (SCFAs) in patients with hypertensive intracerebral hemorrhage and their correlations with the poor outcomes.Methods:Thirty-eight patients with hypertensive intracerebral hemorrhage within 7 d of onset and 32 healthy controls were enrolled prospectively. Fecal samples were collected for 16S rRNA sequencing and SCFAs levels detection. The outcome was evaluated by the modified Rankin Scale at 90 d after the onset, and >2 points were defined as a poor outcome. Multivariate logistic regression model was used to determine the correlations between the gut microbiota and the fecal SCFAs levels and outcomes. Results:The gut microbiota of patients with hypertensive intracerebral hemorrhage was significantly different from that of healthy control group. It is manifested as a decrease in α diversity, a difference in β diversity, an increase in the abundance of potential undesirable bacteria, a decrease in the abundance of common SCFA-producing bacteria and a decrease in the fecal SCFAs levels. In patients with hypertensive intracerebral hemorrhage, compared with the good outcome group, the α diversity of the gut microbiota, the abundance of SCFA-producing bacteria such as Lacetospirillum and Bacteroides, and the total SCFAs, acetic acid and propionic acid levels decreased in the poor outcome group. Multivariate logistic regression analysis showed that after adjusting for potential confounding factors, the decrease of fecal SCFAs levels after log2 conversion was significantly and independently correlated with the poor outcomes. Conclusion:Patients with hypertensive intracerebral hemorrhage have gut microbiota and SCFAs metabolic disorder, the latter is significantly correlated with the poor outcomes. Gut microbiota and SCFAs may become an outcome marker and treatment target for patients with hypertensive intracerebral hemorrhage

Objective:To explore the application and choice of trunk perforator flaps in chest wound repair.Methods:From May 2015 to December 2019, clinical data of 14 patients using trunk perforator flap for chest soft tissue defect repair were reviewed. They were 8 males and 6 females, aged from 17 to 74 years. 5 patients had wound after scar surgery. 6 patients had benign lesions, and 3 patients had malignant tumors. The size of tissue defects ranged from 6.0 cm×8.0 cm-20.0 cm×21.0 cm. The perforator flaps were designed to repair different wounds. Preoperatively, the perforators are identified by ultrasound or CTA, and the flap scope and surgical approach are designed according to the perforator alignment and the perforated muscle point. Postoperatively, the survival of the flap was observed and followed up for at least 6 months.Results:All 17 flaps of 14 patients survived. The flap area was 7.0 cm × 8.0 cm-22.0 cm × 22.0 cm, and the length of the pedicle was 2.0-6.0 cm. All patients were followed up for 6 to 26 months with satisfied result . Recurrence occurred in one of the patients with thoracic dermatofibrosarcoma protuberans.Conclusions:The perforator artery of the trunk is densely and easy to obtain. The combined flaps could be used to repair large chest defects. The first-stage suture of the donor area, so it meets the organization transplantation principle of "the repair is perfect, the damage is small" .

Objective:To explore the application and choice of trunk perforator flaps in chest wound repair.Methods:From May 2015 to December 2019, clinical data of 14 patients using trunk perforator flap for chest soft tissue defect repair were reviewed. They were 8 males and 6 females, aged from 17 to 74 years. 5 patients had wound after scar surgery. 6 patients had benign lesions, and 3 patients had malignant tumors. The size of tissue defects ranged from 6.0 cm×8.0 cm-20.0 cm×21.0 cm. The perforator flaps were designed to repair different wounds. Preoperatively, the perforators are identified by ultrasound or CTA, and the flap scope and surgical approach are designed according to the perforator alignment and the perforated muscle point. Postoperatively, the survival of the flap was observed and followed up for at least 6 months.Results:All 17 flaps of 14 patients survived. The flap area was 7.0 cm × 8.0 cm-22.0 cm × 22.0 cm, and the length of the pedicle was 2.0-6.0 cm. All patients were followed up for 6 to 26 months with satisfied result . Recurrence occurred in one of the patients with thoracic dermatofibrosarcoma protuberans.Conclusions:The perforator artery of the trunk is densely and easy to obtain. The combined flaps could be used to repair large chest defects. The first-stage suture of the donor area, so it meets the organization transplantation principle of "the repair is perfect, the damage is small" .

Objective: To investigate whether N6-methyladenine DNA(6-mA DNA) modification is related to the occurrence of infantile hemangiomas (IH) at the epigenetic level. Methods: The genomic 6-mA DNA data were obtained by MeDIP and high-throughput sequencing. The 6-mA DNA methylation levels in 3 proliferative hemangioma specimens and adjacent skin tissues were compared by u-test. The functional differences of 6-mA modified genes were analyzed by GO analysis. Results: The level of 6-mA DNA modification in IH tissue was higher. The coverage of 6-mA Peaks in the genome was 0.037% in the tumor tissue, and the coverage of 6-mA Peaks in the surrounding skin tissue was 0.013% in the genome. The difference between the two groups was statistically significant (u=5999.87, P=0.00). The gene functions of differentially 6-mA modified genes in tumors were enriched in mesoderm development, stem cell differentiation, mesenchymal development, and cell cycle. These gene functions were closely related to the pathogenesis of IH. Conclusion Abnormal 6-mA DNA modification may be one of the pathogenesis of infantile hemangioma.